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The map label for this gene is nac [H]

Identifier: 50084704

GI number: 50084704

Start: 1540516

End: 1541445

Strand: Reverse

Name: nac [H]

Synonym: ACIAD1539

Alternate gene names: 50084704

Gene position: 1541445-1540516 (Counterclockwise)

Preceding gene: 50084705

Following gene: 50084703

Centisome position: 42.83

GC content: 43.12

Gene sequence:

>930_bases
GTGGAACTCAAACAATTAAAGTATTTTATTGCCGTGATCGAGTCTGGAAGTTTGGGTAAAGCTGCAAAAAATCTCGATAT
TGGCACTTCTGCATTAAGTCAGCAAATCTCAAAATTAGAAAGCGAGCTTTCTATTCGCCTTTTACAACGTACTACTTTGG
GTACAGTCCCCACCCCTGCGGGTTTAGCATTTTTTCAACAAGTACAACTGGCGCTTAGGCACCTAGAGCATGCCGTAGAT
TCTGCGCATTCTTCACGCTTAAGTGGTCATGTGACCGTCGGTTTTTCACCAAGTGTGGCGGCTGTGATTGGCACACATTT
TTTAAAACTCATGAGAAACCGTTACCCCGACATTAAAGTCCGTTTAATAGAAGGATTATCGGGTGATTTAAAAGCTTTAG
TTAATGCAAGGCAGCTCGATGTGGCTATCATCTTTCGTGACGATGTCGATCCACAATGGGCTATACAGCCTTTGGTCAAA
GAGCAAATGTTTTTAATCTCGCCTAAAGAAGTGCTGAGTCAGTATCACCTTGAAAGTTGTATCGACACGCAAACCATTAC
GCATACACAGCTTAAAGCACTTCCCCTGATTTTACCGAGCCAACGACATGGCCTGCGCTTGTTGCTCGAACAAAAAATTC
ATCAACTTAAAGTGGCCTATGAGATTGATGGACTGCATTTGCTGATGGAAAGTATCAGCCAACTCGAAATGGCGACTATT
CAGCCTGCGGGTGCCGCATTAAACTCACGCCAAGATTTATGTTTACTACGCGTCGTTGAACCTGCCATTGAGCGTATTAA
CTATTTAATTAGTTTATCTGAAGAAGAACTCTCGCCTGCCACTTTAGCCACTAAAGTGGTGATTCGTGCATGTGTAACCA
ACTTGATTCAAGAAAAGCGCTGGCCCAGTGCGAAGCTTATAAACACTTAA

Upstream 100 bases:

>100_bases
TTGCCTTTATTCTTTCGGCGGTTTACGCAGCATAATAGAACAAAATGCACAGCGATAAACCCGTGTAGAATAATAAACAA
AGGACAATGTAGTATTTGCC

Downstream 100 bases:

>100_bases
GCCATTTGTATTTATTTTTACTAAACACCCATTCAAGCTGAGCCTCTCACTGCATATTGCCTTTTAAAGCTACATTAGCC
TGAGCTAAAAAAGGAAGAAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MELKQLKYFIAVIESGSLGKAAKNLDIGTSALSQQISKLESELSIRLLQRTTLGTVPTPAGLAFFQQVQLALRHLEHAVD
SAHSSRLSGHVTVGFSPSVAAVIGTHFLKLMRNRYPDIKVRLIEGLSGDLKALVNARQLDVAIIFRDDVDPQWAIQPLVK
EQMFLISPKEVLSQYHLESCIDTQTITHTQLKALPLILPSQRHGLRLLLEQKIHQLKVAYEIDGLHLLMESISQLEMATI
QPAGAALNSRQDLCLLRVVEPAIERINYLISLSEEELSPATLATKVVIRACVTNLIQEKRWPSAKLINT

Sequences:

>Translated_309_residues
MELKQLKYFIAVIESGSLGKAAKNLDIGTSALSQQISKLESELSIRLLQRTTLGTVPTPAGLAFFQQVQLALRHLEHAVD
SAHSSRLSGHVTVGFSPSVAAVIGTHFLKLMRNRYPDIKVRLIEGLSGDLKALVNARQLDVAIIFRDDVDPQWAIQPLVK
EQMFLISPKEVLSQYHLESCIDTQTITHTQLKALPLILPSQRHGLRLLLEQKIHQLKVAYEIDGLHLLMESISQLEMATI
QPAGAALNSRQDLCLLRVVEPAIERINYLISLSEEELSPATLATKVVIRACVTNLIQEKRWPSAKLINT
>Mature_309_residues
MELKQLKYFIAVIESGSLGKAAKNLDIGTSALSQQISKLESELSIRLLQRTTLGTVPTPAGLAFFQQVQLALRHLEHAVD
SAHSSRLSGHVTVGFSPSVAAVIGTHFLKLMRNRYPDIKVRLIEGLSGDLKALVNARQLDVAIIFRDDVDPQWAIQPLVK
EQMFLISPKEVLSQYHLESCIDTQTITHTQLKALPLILPSQRHGLRLLLEQKIHQLKVAYEIDGLHLLMESISQLEMATI
QPAGAALNSRQDLCLLRVVEPAIERINYLISLSEEELSPATLATKVVIRACVTNLIQEKRWPSAKLINT

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=314, Percent_Identity=29.9363057324841, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1786401, Length=185, Percent_Identity=29.1891891891892, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790399, Length=167, Percent_Identity=27.5449101796407, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787879, Length=131, Percent_Identity=36.6412213740458, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI157672245, Length=273, Percent_Identity=24.9084249084249, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787601, Length=170, Percent_Identity=27.0588235294118, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI145693105, Length=299, Percent_Identity=25.752508361204, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789639, Length=182, Percent_Identity=30.2197802197802, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788887, Length=172, Percent_Identity=28.4883720930233, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34336; Mature: 34336

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKQLKYFIAVIESGSLGKAAKNLDIGTSALSQQISKLESELSIRLLQRTTLGTVPTPA
CCHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
GLAFFQQVQLALRHLEHAVDSAHSSRLSGHVTVGFSPSVAAVIGTHFLKLMRNRYPDIKV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCEEE
RLIEGLSGDLKALVNARQLDVAIIFRDDVDPQWAIQPLVKEQMFLISPKEVLSQYHLESC
EEECCCCHHHHHHHCHHHEEEEEEEECCCCCHHHHHHHHHHHHHEECHHHHHHHHHHHHH
IDTQTITHTQLKALPLILPSQRHGLRLLLEQKIHQLKVAYEIDGLHLLMESISQLEMATI
HCCHHHHHHHHHHHCEECCCCCCHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHC
QPAGAALNSRQDLCLLRVVEPAIERINYLISLSEEELSPATLATKVVIRACVTNLIQEKR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHCC
WPSAKLINT
CCCCCCCCC
>Mature Secondary Structure
MELKQLKYFIAVIESGSLGKAAKNLDIGTSALSQQISKLESELSIRLLQRTTLGTVPTPA
CCHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
GLAFFQQVQLALRHLEHAVDSAHSSRLSGHVTVGFSPSVAAVIGTHFLKLMRNRYPDIKV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCEEE
RLIEGLSGDLKALVNARQLDVAIIFRDDVDPQWAIQPLVKEQMFLISPKEVLSQYHLESC
EEECCCCHHHHHHHCHHHEEEEEEEECCCCCHHHHHHHHHHHHHEECHHHHHHHHHHHHH
IDTQTITHTQLKALPLILPSQRHGLRLLLEQKIHQLKVAYEIDGLHLLMESISQLEMATI
HCCHHHHHHHHHHHCEECCCCCCHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHC
QPAGAALNSRQDLCLLRVVEPAIERINYLISLSEEELSPATLATKVVIRACVTNLIQEKR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHCC
WPSAKLINT
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9495755; 9097040; 9278503; 8529872 [H]