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The map label for this gene is gsiB [H]

Identifier: 50084697

GI number: 50084697

Start: 1529494

End: 1531077

Strand: Reverse

Name: gsiB [H]

Synonym: ACIAD1531

Alternate gene names: 50084697

Gene position: 1531077-1529494 (Counterclockwise)

Preceding gene: 50084698

Following gene: 50084692

Centisome position: 42.55

GC content: 41.6

Gene sequence:

>1584_bases
ATGACAAATCAAACATTTCAGTTATCACGTCGAAAATTTTTAACACAAAGTCTCACGGCGCTAGGTGGCGTCTCTTTGAT
GGGCAGTTTATCAGGTTGTGGCCCCTCTCCAGATAATAGTAACGAATCAAAAAGAGCTTCTTCAGCAACACCTAAGCATG
GAGGAGTTATACGCTTGGGACTGATTGGAGGACAACAATCAGGCAGTCTAGATCCTCATCTTTCAGCATCTAGTAGTGGT
ATTACACGAGGTTTCGCTATTTATAATAAGTTATGGGAATGGGATGAAAATATGCTGCCCCGACTTGCTTTAGCGGAGTT
TGCCGAGCCTAATCACAATGCAAGCGAATGGACCATCCGTTTACGCAAAGGCTTAGAGTTTCATCACGGTAAAACCATTA
CCGCTGATGATGTGATCTTTTCAGTGAAACGCCTAACAGACCCTAAACTCGCTTCACCATTTCGTAATCTGGTGCAGTGG
ATTGATCGAGATCGTATTCAAAAACTCGATGAATACACAGTTCGTATTCCTTTTATTCGAACGATTCTCGGCTTTGTGGC
ACTGCCCGAAACTTGGGTAAACTTTGGAGGAATTGTACCGACCGACTTCGACCCTATTCATAATCCAGTCGGGGCTGGTC
CATATAAAGTTCAGGAGTTTATTCCAGGGCAGCGTACTGTTTTTACCCGTTTTAATAACTATTTTAAAGCTGACAAACCT
TATGCAGATCGTTTTGAAGTGATTGATTTTAAAGATCAGGTCTCTCGATTAAATGCTTTATTGGCTGGGCAAATTGATGT
CGCAAATGCGATTTCTCCTGAGTATATTAAAATTTTGGAGCAAGCAAAACACATTCAAACCATACGCTCTGAGACCAATA
CACATAATAGTTTTGACTTTAATACTCAACAAGCGCCGTTTAATGACCCTAGAGTGCGTCAGGCATTTCGTTTGATTGCA
AACCGGGAGGAGTTGGTACAACGAGGCTTAAATGGACAAGGTCGAATCGCCAACGATCTTTATTCGCCGCAAGATCCTGC
CTTTCTCAATTTGCCACAGCGCCAACAAAATATTGCCGAGGCAAAAAATCTATTAGCGCAAGCAGGTTTTAAAAATGGTT
TAAGTGTAGAGCTTGTTACTCCAGTCAGTAGTGCTCAATCTGCACTAATTTTTGCCGAGCAAGCGAAACAAGCCAATGTA
AATATTCGAGTGAAACAAGTTGATTTTGCAACGTTTAATGGTCCTAATCGCAGTAAATGGCAACTATCGAGTAATGCGAC
TAATGTTGGCACACCCTATTTATCAACTGCCGTTGTTAACGATGCCCCAATTTCCACGACGAATCGTGTGAACTTTAAAG
ACCCTGAATACAGTGAATTGTTTTTTAAAGCAATAGCTGAGCCAGATTTAGCCAAACGCAAAATCTATTTAGCTCAAGCA
CAAAGAATTCAGCATGAACGTGGTGGCATGCTCATTTGGGGGTTTAGTCATACCATTGACGCTGCAGCTAAAAATATTGG
TGGTTTAGCTTCCGAACATACGATTTTTCCAACTTGGCGATTTGAGAAACTCTGGAAAGCTTAA

Upstream 100 bases:

>100_bases
TAATTATATAAGTTGTTGATAAATATATTAAAATTATATAAAAATATTGGCATTTATTCTGCAATACCTCAGAAGATGAA
GATCTAATCAGGTATTTATA

Downstream 100 bases:

>100_bases
TACTCATCTGTAAACTCACTATATAAAAAAAGCTGCCGAAGCAGCTTTTTTTATGGAGACATAATTATTTAGGCACTGTA
ATCACAGAGAGTTTATCTAC

Product: oligopeptide binding protein ABC transporter periplasmic substrate-binding protein OppA

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MTNQTFQLSRRKFLTQSLTALGGVSLMGSLSGCGPSPDNSNESKRASSATPKHGGVIRLGLIGGQQSGSLDPHLSASSSG
ITRGFAIYNKLWEWDENMLPRLALAEFAEPNHNASEWTIRLRKGLEFHHGKTITADDVIFSVKRLTDPKLASPFRNLVQW
IDRDRIQKLDEYTVRIPFIRTILGFVALPETWVNFGGIVPTDFDPIHNPVGAGPYKVQEFIPGQRTVFTRFNNYFKADKP
YADRFEVIDFKDQVSRLNALLAGQIDVANAISPEYIKILEQAKHIQTIRSETNTHNSFDFNTQQAPFNDPRVRQAFRLIA
NREELVQRGLNGQGRIANDLYSPQDPAFLNLPQRQQNIAEAKNLLAQAGFKNGLSVELVTPVSSAQSALIFAEQAKQANV
NIRVKQVDFATFNGPNRSKWQLSSNATNVGTPYLSTAVVNDAPISTTNRVNFKDPEYSELFFKAIAEPDLAKRKIYLAQA
QRIQHERGGMLIWGFSHTIDAAAKNIGGLASEHTIFPTWRFEKLWKA

Sequences:

>Translated_527_residues
MTNQTFQLSRRKFLTQSLTALGGVSLMGSLSGCGPSPDNSNESKRASSATPKHGGVIRLGLIGGQQSGSLDPHLSASSSG
ITRGFAIYNKLWEWDENMLPRLALAEFAEPNHNASEWTIRLRKGLEFHHGKTITADDVIFSVKRLTDPKLASPFRNLVQW
IDRDRIQKLDEYTVRIPFIRTILGFVALPETWVNFGGIVPTDFDPIHNPVGAGPYKVQEFIPGQRTVFTRFNNYFKADKP
YADRFEVIDFKDQVSRLNALLAGQIDVANAISPEYIKILEQAKHIQTIRSETNTHNSFDFNTQQAPFNDPRVRQAFRLIA
NREELVQRGLNGQGRIANDLYSPQDPAFLNLPQRQQNIAEAKNLLAQAGFKNGLSVELVTPVSSAQSALIFAEQAKQANV
NIRVKQVDFATFNGPNRSKWQLSSNATNVGTPYLSTAVVNDAPISTTNRVNFKDPEYSELFFKAIAEPDLAKRKIYLAQA
QRIQHERGGMLIWGFSHTIDAAAKNIGGLASEHTIFPTWRFEKLWKA
>Mature_526_residues
TNQTFQLSRRKFLTQSLTALGGVSLMGSLSGCGPSPDNSNESKRASSATPKHGGVIRLGLIGGQQSGSLDPHLSASSSGI
TRGFAIYNKLWEWDENMLPRLALAEFAEPNHNASEWTIRLRKGLEFHHGKTITADDVIFSVKRLTDPKLASPFRNLVQWI
DRDRIQKLDEYTVRIPFIRTILGFVALPETWVNFGGIVPTDFDPIHNPVGAGPYKVQEFIPGQRTVFTRFNNYFKADKPY
ADRFEVIDFKDQVSRLNALLAGQIDVANAISPEYIKILEQAKHIQTIRSETNTHNSFDFNTQQAPFNDPRVRQAFRLIAN
REELVQRGLNGQGRIANDLYSPQDPAFLNLPQRQQNIAEAKNLLAQAGFKNGLSVELVTPVSSAQSALIFAEQAKQANVN
IRVKQVDFATFNGPNRSKWQLSSNATNVGTPYLSTAVVNDAPISTTNRVNFKDPEYSELFFKAIAEPDLAKRKIYLAQAQ
RIQHERGGMLIWGFSHTIDAAAKNIGGLASEHTIFPTWRFEKLWKA

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=478, Percent_Identity=24.0585774058577, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1787052, Length=356, Percent_Identity=24.7191011235955, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789966, Length=520, Percent_Identity=23.8461538461538, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1787551, Length=518, Percent_Identity=23.9382239382239, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081878, Length=422, Percent_Identity=22.5118483412322, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1789887, Length=321, Percent_Identity=25.8566978193146, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI1789397, Length=481, Percent_Identity=21.8295218295218, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58923; Mature: 58792

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNQTFQLSRRKFLTQSLTALGGVSLMGSLSGCGPSPDNSNESKRASSATPKHGGVIRLG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCEEEEE
LIGGQQSGSLDPHLSASSSGITRGFAIYNKLWEWDENMLPRLALAEFAEPNHNASEWTIR
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHEEEE
LRKGLEFHHGKTITADDVIFSVKRLTDPKLASPFRNLVQWIDRDRIQKLDEYTVRIPFIR
EHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHH
TILGFVALPETWVNFGGIVPTDFDPIHNPVGAGPYKVQEFIPGQRTVFTRFNNYFKADKP
HHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCC
YADRFEVIDFKDQVSRLNALLAGQIDVANAISPEYIKILEQAKHIQTIRSETNTHNSFDF
CCCCEEEECHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NTQQAPFNDPRVRQAFRLIANREELVQRGLNGQGRIANDLYSPQDPAFLNLPQRQQNIAE
CCCCCCCCCHHHHHHHHHHHCHHHHHHCCCCCCCCCHHHCCCCCCCCEECCCHHHHHHHH
AKNLLAQAGFKNGLSVELVTPVSSAQSALIFAEQAKQANVNIRVKQVDFATFNGPNRSKW
HHHHHHHHCCCCCCEEEEECCCCCCHHHHEEHHHHHCCCCEEEEEEEEEEEECCCCCCEE
QLSSNATNVGTPYLSTAVVNDAPISTTNRVNFKDPEYSELFFKAIAEPDLAKRKIYLAQA
EECCCCCCCCCCCHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
QRIQHERGGMLIWGFSHTIDAAAKNIGGLASEHTIFPTWRFEKLWKA
HHHHHHCCCEEEEECHHHHHHHHHHHCCCCCCCEECCCHHHHHHHCC
>Mature Secondary Structure 
TNQTFQLSRRKFLTQSLTALGGVSLMGSLSGCGPSPDNSNESKRASSATPKHGGVIRLG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCEEEEE
LIGGQQSGSLDPHLSASSSGITRGFAIYNKLWEWDENMLPRLALAEFAEPNHNASEWTIR
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHEEEE
LRKGLEFHHGKTITADDVIFSVKRLTDPKLASPFRNLVQWIDRDRIQKLDEYTVRIPFIR
EHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHH
TILGFVALPETWVNFGGIVPTDFDPIHNPVGAGPYKVQEFIPGQRTVFTRFNNYFKADKP
HHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCC
YADRFEVIDFKDQVSRLNALLAGQIDVANAISPEYIKILEQAKHIQTIRSETNTHNSFDF
CCCCEEEECHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NTQQAPFNDPRVRQAFRLIANREELVQRGLNGQGRIANDLYSPQDPAFLNLPQRQQNIAE
CCCCCCCCCHHHHHHHHHHHCHHHHHHCCCCCCCCCHHHCCCCCCCCEECCCHHHHHHHH
AKNLLAQAGFKNGLSVELVTPVSSAQSALIFAEQAKQANVNIRVKQVDFATFNGPNRSKW
HHHHHHHHCCCCCCEEEEECCCCCCHHHHEEHHHHHCCCCEEEEEEEEEEEECCCCCCEE
QLSSNATNVGTPYLSTAVVNDAPISTTNRVNFKDPEYSELFFKAIAEPDLAKRKIYLAQA
EECCCCCCCCCCCHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
QRIQHERGGMLIWGFSHTIDAAAKNIGGLASEHTIFPTWRFEKLWKA
HHHHHHCCCEEEEECHHHHHHHHHHHCCCCCCCEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA