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The map label for this gene is cysS

Identifier: 50084652

GI number: 50084652

Start: 1479012

End: 1480433

Strand: Reverse

Name: cysS

Synonym: ACIAD1481

Alternate gene names: 50084652

Gene position: 1480433-1479012 (Counterclockwise)

Preceding gene: 50084663

Following gene: 50084642

Centisome position: 41.14

GC content: 41.42

Gene sequence:

>1422_bases
ATGCAACCATTTGTATTATATAACTCCGAGCAACGTAAGAAAGTTGAATTTGTCCCTCGTAAACCTGGGCATATCGATAT
GTATGTCTGCGGTATGACAGTATATGACTATTGTCATATTGGGCACGCTCGGGTAATGGTTGCATTTGACTATATTATTC
GTTTCTTACGTAGCCAAGGCTGGGATGTCCGTTATATCCGTAACATTACCGATATTGACGATAAAATCATCAAACGTGCT
AATGAAAACAATGAAACCATTCAGCAGTTAACCAGTCGATTTATCGATGCAATGAACGAAGACGCAGCTAAACTGGGCTG
TGCCGAACCTGATGAAGCGCCTAAAGCGACTGAATATATCGGCGAAATGCAAAATATGATTAGCACGCTCGTTGACAAAG
GAAGTGCTTATCCAGCTTCAAATGGTGACGTTTATTTTGAAGTCAGTAAGTTTGAAAAGTATGGTCGTTTGTCAGGTCGC
AAGCTCGAAGACATGCAAGCGGGTGCCAGCGAGCGTGTAGATATCGAGGTAGAGAAAAAACATCCATTTGACTTTGTACT
TTGGAAAGGCGCCAAGCCAAATGAACCTTCTTGGGTATCACCTTGGGGAAATGGCCGCCCAGGCTGGCATATTGAATGTT
CCGCAATGTCAACTTGCTGCTTAGGTAATCATTTTGATATCCATGGCGGTGGATCTGATTTGACCTTCCCGCATCATGAA
AATGAAATTGCGCAAAGTGAGGCTTCTACTGGCGAGCAATATGTCAACTACTGGATGCATGTTGGTTTTATCAATGTTGA
TGGTGAGAAAATGTCTAAGTCCTTAGGCAACTTTTTCACCATTCGTGATGTGATGGAGAAGTTCCATCCTGAGGTCATTC
GCTACTTTATTGTATCTTCACATTATCGTAGTCCAGTAAACTTCTCTGACGTCGCTCTAAAAGAAGCCAAAACTGCTCTA
ACACGTTTTTACCATGCATTTAAAGCATATGAGCAGGTATATGGACAAAATGACAGTACACAAAAAGACGAACAATTCAT
CGAGCGCTTTAATATCGCAATGCGTGATGATTTCAATACGCCTGAGGCTATAGCGGTATTATTTGAACTCAACCGTGAAC
TGAATCGTGCTGTAAAAGAGCAACAAGCTGAACAAGCAGCTATATATTATGTAACTTTACGTCATCTCACTCAAATTTTG
GGTCTAGTGCAACACGACGTTGATGAATTTTTAAAATCGGATATTGGACAAGAAGTGTTAAGCCTCTCTGATGATGAAAT
TCAGGCAATGATTCAACAGCGTGCAGATGCTAAAAAAGCGAAAGATTTTTCAGGGGCTGATGCAATTCGTCAAGCACTTT
TGGATCAAGGTGTTGTCCTTGAAGATACCCGTCAAGGTACATTATGGCGACGTGCTGATTAA

Upstream 100 bases:

>100_bases
ACTTTTCACAAGCTTATAAAATTCGGCGATAAATCTTTGTTAAAAATGTAGCTTACGTTATGATGTAACACGATTTTTGC
TGAGTATTTTGAAGAACTTT

Downstream 100 bases:

>100_bases
TTCGGCAGTCGGTATAATTGTATATTGACACTCAAAGCAAAATGTCTATAATGCATCACACATTGCGGGAATAGCTCAGT
TGGTAGAGCACAACCTTGCC

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MQPFVLYNSEQRKKVEFVPRKPGHIDMYVCGMTVYDYCHIGHARVMVAFDYIIRFLRSQGWDVRYIRNITDIDDKIIKRA
NENNETIQQLTSRFIDAMNEDAAKLGCAEPDEAPKATEYIGEMQNMISTLVDKGSAYPASNGDVYFEVSKFEKYGRLSGR
KLEDMQAGASERVDIEVEKKHPFDFVLWKGAKPNEPSWVSPWGNGRPGWHIECSAMSTCCLGNHFDIHGGGSDLTFPHHE
NEIAQSEASTGEQYVNYWMHVGFINVDGEKMSKSLGNFFTIRDVMEKFHPEVIRYFIVSSHYRSPVNFSDVALKEAKTAL
TRFYHAFKAYEQVYGQNDSTQKDEQFIERFNIAMRDDFNTPEAIAVLFELNRELNRAVKEQQAEQAAIYYVTLRHLTQIL
GLVQHDVDEFLKSDIGQEVLSLSDDEIQAMIQQRADAKKAKDFSGADAIRQALLDQGVVLEDTRQGTLWRRAD

Sequences:

>Translated_473_residues
MQPFVLYNSEQRKKVEFVPRKPGHIDMYVCGMTVYDYCHIGHARVMVAFDYIIRFLRSQGWDVRYIRNITDIDDKIIKRA
NENNETIQQLTSRFIDAMNEDAAKLGCAEPDEAPKATEYIGEMQNMISTLVDKGSAYPASNGDVYFEVSKFEKYGRLSGR
KLEDMQAGASERVDIEVEKKHPFDFVLWKGAKPNEPSWVSPWGNGRPGWHIECSAMSTCCLGNHFDIHGGGSDLTFPHHE
NEIAQSEASTGEQYVNYWMHVGFINVDGEKMSKSLGNFFTIRDVMEKFHPEVIRYFIVSSHYRSPVNFSDVALKEAKTAL
TRFYHAFKAYEQVYGQNDSTQKDEQFIERFNIAMRDDFNTPEAIAVLFELNRELNRAVKEQQAEQAAIYYVTLRHLTQIL
GLVQHDVDEFLKSDIGQEVLSLSDDEIQAMIQQRADAKKAKDFSGADAIRQALLDQGVVLEDTRQGTLWRRAD
>Mature_473_residues
MQPFVLYNSEQRKKVEFVPRKPGHIDMYVCGMTVYDYCHIGHARVMVAFDYIIRFLRSQGWDVRYIRNITDIDDKIIKRA
NENNETIQQLTSRFIDAMNEDAAKLGCAEPDEAPKATEYIGEMQNMISTLVDKGSAYPASNGDVYFEVSKFEKYGRLSGR
KLEDMQAGASERVDIEVEKKHPFDFVLWKGAKPNEPSWVSPWGNGRPGWHIECSAMSTCCLGNHFDIHGGGSDLTFPHHE
NEIAQSEASTGEQYVNYWMHVGFINVDGEKMSKSLGNFFTIRDVMEKFHPEVIRYFIVSSHYRSPVNFSDVALKEAKTAL
TRFYHAFKAYEQVYGQNDSTQKDEQFIERFNIAMRDDFNTPEAIAVLFELNRELNRAVKEQQAEQAAIYYVTLRHLTQIL
GLVQHDVDEFLKSDIGQEVLSLSDDEIQAMIQQRADAKKAKDFSGADAIRQALLDQGVVLEDTRQGTLWRRAD

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=479, Percent_Identity=36.3256784968685, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI303304987, Length=441, Percent_Identity=30.8390022675737, Blast_Score=195, Evalue=9e-50,
Organism=Homo sapiens, GI62240992, Length=372, Percent_Identity=32.258064516129, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI21269877, Length=441, Percent_Identity=30.8390022675737, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI10835051, Length=372, Percent_Identity=32.258064516129, Blast_Score=194, Evalue=1e-49,
Organism=Escherichia coli, GI1786737, Length=466, Percent_Identity=53.0042918454936, Blast_Score=474, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI17509797, Length=233, Percent_Identity=39.4849785407725, Blast_Score=181, Evalue=9e-46,
Organism=Caenorhabditis elegans, GI17509795, Length=233, Percent_Identity=39.4849785407725, Blast_Score=181, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6324082, Length=356, Percent_Identity=32.3033707865169, Blast_Score=204, Evalue=2e-53,
Organism=Drosophila melanogaster, GI19922218, Length=499, Percent_Identity=33.2665330661323, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI19922364, Length=271, Percent_Identity=36.5313653136531, Blast_Score=183, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_ACIAD (Q6FC71)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_046162.1
- ProteinModelPortal:   Q6FC71
- SMR:   Q6FC71
- STRING:   Q6FC71
- GeneID:   2878449
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1481
- NMPDR:   fig|62977.3.peg.1514
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- PhylomeDB:   Q6FC71
- ProtClustDB:   PRK00260
- BioCyc:   ASP62977:ACIAD1481-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 54232; Mature: 54232

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 273-273

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPFVLYNSEQRKKVEFVPRKPGHIDMYVCGMTVYDYCHIGHARVMVAFDYIIRFLRSQG
CCCEEEECCCCCCHHCCCCCCCCCEEEEEECHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
WDVRYIRNITDIDDKIIKRANENNETIQQLTSRFIDAMNEDAAKLGCAEPDEAPKATEYI
CCEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHH
GEMQNMISTLVDKGSAYPASNGDVYFEVSKFEKYGRLSGRKLEDMQAGASERVDIEVEKK
HHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHCCCCCCHHHHHCCCCCCEEEEEECC
HPFDFVLWKGAKPNEPSWVSPWGNGRPGWHIECSAMSTCCLGNHFDIHGGGSDLTFPHHE
CCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCEEECCCCCCCCCCCCC
NEIAQSEASTGEQYVNYWMHVGFINVDGEKMSKSLGNFFTIRDVMEKFHPEVIRYFIVSS
HHHHHHHHCCHHHHHHHHHHEEEEEECHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
HYRSPVNFSDVALKEAKTALTRFYHAFKAYEQVYGQNDSTQKDEQFIERFNIAMRDDFNT
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHCCCCCC
PEAIAVLFELNRELNRAVKEQQAEQAAIYYVTLRHLTQILGLVQHDVDEFLKSDIGQEVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLSDDEIQAMIQQRADAKKAKDFSGADAIRQALLDQGVVLEDTRQGTLWRRAD
HCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCEECCC
>Mature Secondary Structure
MQPFVLYNSEQRKKVEFVPRKPGHIDMYVCGMTVYDYCHIGHARVMVAFDYIIRFLRSQG
CCCEEEECCCCCCHHCCCCCCCCCEEEEEECHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
WDVRYIRNITDIDDKIIKRANENNETIQQLTSRFIDAMNEDAAKLGCAEPDEAPKATEYI
CCEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHH
GEMQNMISTLVDKGSAYPASNGDVYFEVSKFEKYGRLSGRKLEDMQAGASERVDIEVEKK
HHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHCCCCCCHHHHHCCCCCCEEEEEECC
HPFDFVLWKGAKPNEPSWVSPWGNGRPGWHIECSAMSTCCLGNHFDIHGGGSDLTFPHHE
CCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCEEECCCCCCCCCCCCC
NEIAQSEASTGEQYVNYWMHVGFINVDGEKMSKSLGNFFTIRDVMEKFHPEVIRYFIVSS
HHHHHHHHCCHHHHHHHHHHEEEEEECHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
HYRSPVNFSDVALKEAKTALTRFYHAFKAYEQVYGQNDSTQKDEQFIERFNIAMRDDFNT
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHCCCCCC
PEAIAVLFELNRELNRAVKEQQAEQAAIYYVTLRHLTQILGLVQHDVDEFLKSDIGQEVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLSDDEIQAMIQQRADAKKAKDFSGADAIRQALLDQGVVLEDTRQGTLWRRAD
HCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA