Click here to switch to the map view.

The map label for this gene is catJ

Identifier: 50084624

GI number: 50084624

Start: 1446922

End: 1447575

Strand: Direct

Name: catJ

Synonym: ACIAD1449

Alternate gene names: 50084624

Gene position: 1446922-1447575 (Clockwise)

Preceding gene: 50084623

Following gene: 50084625

Centisome position: 40.21

GC content: 53.36

Gene sequence:

>654_bases
ATGAGTTATCACAAACTGACCCGTGACCAGATCGCCCAGCGCGTTGCCCAAGACATTCCGGAAGGCTCCTATGTCAATCT
TGGCATTGGCCTGCCGACCAAGATTGCCAGCTATCTGCCTGCCGACAAAGACGTATTTCTACATTCAGAAAACGGACTGC
TGGCCTTTGGCCCACCACCAGCGGCCGGCGAAGAAGATCCGGAACTGATCAACGCAGGCAAAGAATACGTAACCATGCTC
GAAGGCGGTTGCTTCTTTCACCATGGCGACTCCTTCGCCATGATGCGCGGTGGACATCTGGATATCTGCGTATTAGGCGC
ATTCCAGATCGCCGCCAATGGAGACCTGGCCAACTGGCACACCGGTGCACCGGATGCCATACCGTCGGTCGGTGGAGCCA
TGGATCTTGCGGTTGGGGCAAAAAAAGTTTTTGTAACCACCGATCATGTCACCAAAAAAGGTGAGCCGAAGATTGTAGCT
GAACTGACGTATCCAGCCACGGGTCAGAAATGTGTCGACCGGATCTACACCGACCTGTGCATCATCGATGTGGTGCCAGA
AGGACTGAAAGTGATCGAGAAAGTCGAAGGCTTAAGCTTTGAAGAACTACAACGCCTGACCGGTGCAACACTGATCGATG
CGACACAAGGCTAA

Upstream 100 bases:

>100_bases
GAACTCGACCCGGAAAACGTGGTGACCCCAGGCATCTTTGTTCAACACGTTGTACCAGTCCAATCTACCCCAGCAAGCGC
TGCACCATAAGGAGATCGAG

Downstream 100 bases:

>100_bases
GGATAACCGAATATGAAACATGCATACATCGTCGATGCGATCCGTACCCCATTTGGCCGCTATGCAGGCGGATTGGCAGC
AGTACGTGCGGATGATCTCG

Product: 3-oxoadipate CoA-transferase subunit B

Products: NA

Alternate protein names: Beta-ketoadipate:succinyl-CoA transferase subunit B

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSYHKLTRDQIAQRVAQDIPEGSYVNLGIGLPTKIASYLPADKDVFLHSENGLLAFGPPPAAGEEDPELINAGKEYVTML
EGGCFFHHGDSFAMMRGGHLDICVLGAFQIAANGDLANWHTGAPDAIPSVGGAMDLAVGAKKVFVTTDHVTKKGEPKIVA
ELTYPATGQKCVDRIYTDLCIIDVVPEGLKVIEKVEGLSFEELQRLTGATLIDATQG

Sequences:

>Translated_217_residues
MSYHKLTRDQIAQRVAQDIPEGSYVNLGIGLPTKIASYLPADKDVFLHSENGLLAFGPPPAAGEEDPELINAGKEYVTML
EGGCFFHHGDSFAMMRGGHLDICVLGAFQIAANGDLANWHTGAPDAIPSVGGAMDLAVGAKKVFVTTDHVTKKGEPKIVA
ELTYPATGQKCVDRIYTDLCIIDVVPEGLKVIEKVEGLSFEELQRLTGATLIDATQG
>Mature_216_residues
SYHKLTRDQIAQRVAQDIPEGSYVNLGIGLPTKIASYLPADKDVFLHSENGLLAFGPPPAAGEEDPELINAGKEYVTMLE
GGCFFHHGDSFAMMRGGHLDICVLGAFQIAANGDLANWHTGAPDAIPSVGGAMDLAVGAKKVFVTTDHVTKKGEPKIVAE
LTYPATGQKCVDRIYTDLCIIDVVPEGLKVIEKVEGLSFEELQRLTGATLIDATQG

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family

Homologues:

Organism=Homo sapiens, GI11545841, Length=206, Percent_Identity=45.1456310679612, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI4557817, Length=203, Percent_Identity=45.8128078817734, Blast_Score=175, Evalue=3e-44,
Organism=Escherichia coli, GI1788552, Length=202, Percent_Identity=39.6039603960396, Blast_Score=152, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17531517, Length=204, Percent_Identity=45.5882352941176, Blast_Score=169, Evalue=9e-43,
Organism=Drosophila melanogaster, GI21358325, Length=209, Percent_Identity=43.0622009569378, Blast_Score=171, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24655794, Length=209, Percent_Identity=43.0622009569378, Blast_Score=171, Evalue=3e-43,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PCAJ_ACIAD (Q59091)

Other databases:

- EMBL:   L05770
- EMBL:   AF009224
- EMBL:   CR543861
- EMBL:   CR543861
- PIR:   B44570
- RefSeq:   YP_046134.1
- RefSeq:   YP_046369.1
- ProteinModelPortal:   Q59091
- SMR:   Q59091
- STRING:   Q59091
- GeneID:   2879439
- GeneID:   2879925
- GenomeReviews:   CR543861_GR
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1449
- KEGG:   aci:ACIAD1705
- eggNOG:   COG2057
- HOGENOM:   HBG353770
- OMA:   TWVMMDL
- PhylomeDB:   Q59091
- ProtClustDB:   CLSK707318
- BioCyc:   ASP62977:ACIAD1449-MONOMER
- BioCyc:   ASP62977:ACIAD1705-MONOMER
- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164
- PANTHER:   PTHR13707
- SMART:   SM00882
- TIGRFAMs:   TIGR02428

Pfam domain/function: PF01144 CoA_trans

EC number: =2.8.3.6

Molecular weight: Translated: 23166; Mature: 23034

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS01274 COA_TRANSF_2

Important sites: ACT_SITE 50-50

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYHKLTRDQIAQRVAQDIPEGSYVNLGIGLPTKIASYLPADKDVFLHSENGLLAFGPPP
CCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCCCCEEEECCCCEEEECCCC
AAGEEDPELINAGKEYVTMLEGGCFFHHGDSFAMMRGGHLDICVLGAFQIAANGDLANWH
CCCCCCHHHHHCCHHHHHHHCCCEEEEECCCEEEEECCCEEEEEEEEEEEEECCCCCCCC
TGAPDAIPSVGGAMDLAVGAKKVFVTTDHVTKKGEPKIVAELTYPATGQKCVDRIYTDLC
CCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
IIDVVPEGLKVIEKVEGLSFEELQRLTGATLIDATQG
HEECCCHHHHHHHHHCCCCHHHHHHHHCCEEEECCCC
>Mature Secondary Structure 
SYHKLTRDQIAQRVAQDIPEGSYVNLGIGLPTKIASYLPADKDVFLHSENGLLAFGPPP
CCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCCCCEEEECCCCEEEECCCC
AAGEEDPELINAGKEYVTMLEGGCFFHHGDSFAMMRGGHLDICVLGAFQIAANGDLANWH
CCCCCCHHHHHCCHHHHHHHCCCEEEEECCCEEEEECCCEEEEEEEEEEEEECCCCCCCC
TGAPDAIPSVGGAMDLAVGAKKVFVTTDHVTKKGEPKIVAELTYPATGQKCVDRIYTDLC
CCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
IIDVVPEGLKVIEKVEGLSFEELQRLTGATLIDATQG
HEECCCHHHHHHHHHCCCCHHHHHHHHCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8063101; 8125318