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The map label for this gene is catB

Identifier: 50084621

GI number: 50084621

Start: 1444715

End: 1445827

Strand: Direct

Name: catB

Synonym: ACIAD1446

Alternate gene names: 50084621

Gene position: 1444715-1445827 (Clockwise)

Preceding gene: 50084619

Following gene: 50084622

Centisome position: 40.15

GC content: 44.65

Gene sequence:

>1113_bases
ATGTATAAATCAGTGGAAACTATTTTAGTCGATATTCCAACTATTCGTCCGCACAAGCTTTCGGTCACCACGATGCAGAC
GCAGACATTAGTTTTGATCAAAATCATCACGGAAGATGGCATTGTAGGCTGGGGCGAAGCAACCACGATTGGTGGATTAA
ACTATGGCGAAGAAAGTCCAGAGAGTGTTAAAGCCAATATCGACACCTATTTCAAGCCATTGCTGTTGTCCATCAAGGCG
CCTTTAAATGTGGCTCAGACCCTAAAATTAATTCGTAAAAGTATCAATGGCAACCGCTTTGCCAAATGCGCAATTCAAAC
TGCGCTTTTAGAGATACAGGCTAAACGACTGAATGTACCTGTGAGCGAATTGCTGGGTGGACGTATCCGTGATCGATTAC
CTGTGCTGTGGACACTGGCTTCAGGTGATACCGATAAAGATATTGCCGAAGCCAAAAAAATGATCGAATTAAAGCGCCAC
AATACCTTTAAATTAAAAATTGGTTCAAATCCGCTACAGCATGATGTCGATCATGTAATTGCCATTAAAAAAGCACTTGG
CCCGGAAATCAGTGTGCGTGTAGATGTGAACCGTGCATGGTCTGAGCTTGAATGTGTTAAAGGGATTCAACAGCTTCAAG
ATGGTGGGATCGATCTGATTGAGCAGCCCTGTGCGATTGAAAATACAGATGCTTTAGCACGTCTGACCGCACGTTTTGAT
GTGGCCATTATGGCAGATGAGGTCTTGACTGGCCCAGACAGTGCCTATCGAATTGCCAAAAAATCGGGTGCCGATGTGTT
TGCAGTAAAAGTCGAACAATCGGGTGGTCTGATTGAAGCCTGTGAAGTGGCAAAAATTGCGCGTCTTGCTGGAATCAGTC
TATATGGTGGCACCATGCTTGAAGGGCCTGTCGGCAGTATTGCATCGGCACATGCCTTTAGTACCTTTGAAACACTGGAG
TTTGGAACTGAACTGTTCGGGCCTTTATTACTAACACAAAGCATTTTGAAAACACCTTTGCAATATGAAAACTTCGAGCT
GGTGGTTCCCAATACGCCAGGTCTGGGAATTGAGGTCGACGAAGACAAACTCGAACAGTTACGCCGTCATTAA

Upstream 100 bases:

>100_bases
ATACCTTTTTAGTATGCAAAAATACCAAATTGTTTATCTTTTTTATTATTACATTAATTTAAGGTATGTAAATAGTATTT
ATTGAAAAGAAGATGGACCG

Downstream 100 bases:

>100_bases
GGAAGAGGAAATTTACTATGTTGTTTCAAGTTCGTATGGATGTGCATTTACCTGTTAGCATGCCTACTGATCAGGCCAAT
CAGATTAAATCGGTCGAGAA

Product: muconate cycloisomerase I

Products: NA

Alternate protein names: Cis,cis-muconate lactonizing enzyme I; MLE; Muconate cycloisomerase I

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MYKSVETILVDIPTIRPHKLSVTTMQTQTLVLIKIITEDGIVGWGEATTIGGLNYGEESPESVKANIDTYFKPLLLSIKA
PLNVAQTLKLIRKSINGNRFAKCAIQTALLEIQAKRLNVPVSELLGGRIRDRLPVLWTLASGDTDKDIAEAKKMIELKRH
NTFKLKIGSNPLQHDVDHVIAIKKALGPEISVRVDVNRAWSELECVKGIQQLQDGGIDLIEQPCAIENTDALARLTARFD
VAIMADEVLTGPDSAYRIAKKSGADVFAVKVEQSGGLIEACEVAKIARLAGISLYGGTMLEGPVGSIASAHAFSTFETLE
FGTELFGPLLLTQSILKTPLQYENFELVVPNTPGLGIEVDEDKLEQLRRH

Sequences:

>Translated_370_residues
MYKSVETILVDIPTIRPHKLSVTTMQTQTLVLIKIITEDGIVGWGEATTIGGLNYGEESPESVKANIDTYFKPLLLSIKA
PLNVAQTLKLIRKSINGNRFAKCAIQTALLEIQAKRLNVPVSELLGGRIRDRLPVLWTLASGDTDKDIAEAKKMIELKRH
NTFKLKIGSNPLQHDVDHVIAIKKALGPEISVRVDVNRAWSELECVKGIQQLQDGGIDLIEQPCAIENTDALARLTARFD
VAIMADEVLTGPDSAYRIAKKSGADVFAVKVEQSGGLIEACEVAKIARLAGISLYGGTMLEGPVGSIASAHAFSTFETLE
FGTELFGPLLLTQSILKTPLQYENFELVVPNTPGLGIEVDEDKLEQLRRH
>Mature_370_residues
MYKSVETILVDIPTIRPHKLSVTTMQTQTLVLIKIITEDGIVGWGEATTIGGLNYGEESPESVKANIDTYFKPLLLSIKA
PLNVAQTLKLIRKSINGNRFAKCAIQTALLEIQAKRLNVPVSELLGGRIRDRLPVLWTLASGDTDKDIAEAKKMIELKRH
NTFKLKIGSNPLQHDVDHVIAIKKALGPEISVRVDVNRAWSELECVKGIQQLQDGGIDLIEQPCAIENTDALARLTARFD
VAIMADEVLTGPDSAYRIAKKSGADVFAVKVEQSGGLIEACEVAKIARLAGISLYGGTMLEGPVGSIASAHAFSTFETLE
FGTELFGPLLLTQSILKTPLQYENFELVVPNTPGLGIEVDEDKLEQLRRH

Specific function: Catalyzes a syn cycloisomerization

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family

Homologues:

Organism=Escherichia coli, GI48994953, Length=372, Percent_Identity=23.9247311827957, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CATB_ACIAD (Q43931)

Other databases:

- EMBL:   AF009224
- EMBL:   CR543861
- RefSeq:   YP_046131.1
- ProteinModelPortal:   Q43931
- SMR:   Q43931
- STRING:   Q43931
- GeneID:   2880528
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1446
- NMPDR:   fig|62977.3.peg.803
- eggNOG:   COG4948
- HOGENOM:   HBG450298
- OMA:   AHVIAIK
- ProtClustDB:   CLSK707317
- BioCyc:   ASP62977:ACIAD1446-MONOMER
- InterPro:   IPR013370
- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354
- PANTHER:   PTHR13794
- SMART:   SM00922
- TIGRFAMs:   TIGR02534

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N

EC number: =5.5.1.1

Molecular weight: Translated: 40415; Mature: 40415

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00908 MR_MLE_1; PS00909 MR_MLE_2

Important sites: ACT_SITE 166-166

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKSVETILVDIPTIRPHKLSVTTMQTQTLVLIKIITEDGIVGWGEATTIGGLNYGEESP
CCCCEEEEEEECCCCCCCEEEEEEEECCEEEEEEEEECCCEEECCCCCEECCCCCCCCCC
ESVKANIDTYFKPLLLSIKAPLNVAQTLKLIRKSINGNRFAKCAIQTALLEIQAKRLNVP
HHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
VSELLGGRIRDRLPVLWTLASGDTDKDIAEAKKMIELKRHNTFKLKIGSNPLQHDVDHVI
HHHHHCCHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
AIKKALGPEISVRVDVNRAWSELECVKGIQQLQDGGIDLIEQPCAIENTDALARLTARFD
HHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHE
VAIMADEVLTGPDSAYRIAKKSGADVFAVKVEQSGGLIEACEVAKIARLAGISLYGGTML
EEEEECHHHCCCCHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCEEEECCEEE
EGPVGSIASAHAFSTFETLEFGTELFGPLLLTQSILKTPLQYENFELVVPNTPGLGIEVD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEC
EDKLEQLRRH
HHHHHHHHCC
>Mature Secondary Structure
MYKSVETILVDIPTIRPHKLSVTTMQTQTLVLIKIITEDGIVGWGEATTIGGLNYGEESP
CCCCEEEEEEECCCCCCCEEEEEEEECCEEEEEEEEECCCEEECCCCCEECCCCCCCCCC
ESVKANIDTYFKPLLLSIKAPLNVAQTLKLIRKSINGNRFAKCAIQTALLEIQAKRLNVP
HHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
VSELLGGRIRDRLPVLWTLASGDTDKDIAEAKKMIELKRHNTFKLKIGSNPLQHDVDHVI
HHHHHCCHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
AIKKALGPEISVRVDVNRAWSELECVKGIQQLQDGGIDLIEQPCAIENTDALARLTARFD
HHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHE
VAIMADEVLTGPDSAYRIAKKSGADVFAVKVEQSGGLIEACEVAKIARLAGISLYGGTML
EEEEECHHHCCCCHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCEEEECCEEE
EGPVGSIASAHAFSTFETLEFGTELFGPLLLTQSILKTPLQYENFELVVPNTPGLGIEVD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEC
EDKLEQLRRH
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8125318