Click here to switch to the map view.

The map label for this gene is iscS [H]

Identifier: 50084581

GI number: 50084581

Start: 1398025

End: 1399242

Strand: Reverse

Name: iscS [H]

Synonym: ACIAD1404

Alternate gene names: 50084581

Gene position: 1399242-1398025 (Counterclockwise)

Preceding gene: 50084582

Following gene: 50084580

Centisome position: 38.88

GC content: 41.46

Gene sequence:

>1218_bases
ATGAAACGTCCGATTTATCTTGATTATGCAGCAACTACTCCCGTTCTACCAGAAGTTGCAGAACGCATGATGGAATGTCT
AACTTTTGAAGGGACATTTGGTAATCCTGCATCACGTTCACATGCATATGGCTGGCAAGCTGAAGAAAAAGTTGAATATG
CACGCGAACAAGTTGCCAATCTTATTAAAGCAGACCCTCGTGAGATTGTGTGGACTTCAGGCGCAACTGAATCTGATAAT
TTAGCACTTAAAGGTATTGCACAGTTTTATGGTTCAAAAGGTAAACATATCATTACCAGTAAAATTGAACATAAAGCTGT
TCTAGATCCATGCCGTGAACTTGAAGAAGCTGGCTTTGAAATTACTTATTTAGAACCAGAACCACGCACAGGCCTGATTA
CACCAGAGATGGTTGCTGCTGCATTACGTCCAGATACCATTTTGGTTTCTCTCATGATGGTTAATAATGAAATTGGGACG
ATCACTGATGTTGCTGCAATTGGTGAATTAACTCGTGCCAATAAAACCTTTTTCCATGTCGATGCCGCACAGGCAGCGGG
TAAAGTTGAAATTGATCTTTCAACCATGAAAATTGACTTGATGAGTTTCTCAGCTCATAAAGCTTATGGTCCAAAAGGTA
TTGGTGCCTTGTATGTCCGTCGTACTCCCCGTGTTCGTTTGAAAGCTCAAATACATGGCGGTGGCCATGAACGTGGTATG
CGCTCAGGTACATTAGCAACGCATCAAATCGTGGGTATGGGTGAAGCATTCGAGATTGCAGGTAAAACATTGCATTCTGA
ACAACAGCGTTTACGTAAATTGCGTGACAAGCTTTGGAACGGTCTGCAAGGCTTAGAGCAAGTATTTTTAAATGGTCATC
CAACTCAGAACGTTGCCAATTATTTAAATGTTAGCTTCAACTTTGTTGAAGGTGAATCTTTGATGATGGCACTTAAAGAT
GCAGCAGTATCAAGTGGTTCTGCATGTACATCTGCGACCCTTGAACCCTCTTATGTGCTCCGCGCATTAGGATTATCTGA
TGAACTGGCACACAGTTCTATCCGCTTCAGTTTTGGTAAATACACCACTGAAGAAGATATTGATCATGTACTTGAAGTAA
CCAAAGCTGCTGTAGAGAAATTACGCGAACTTTCTCCGCTTTGGGATATGTATAAAGAAGGTATCGACCTTTCTACAGTT
GAATGGGCTGAACACTAA

Upstream 100 bases:

>100_bases
CTTGCTGACCTGATTGCTCGCGATAATGTGCAAACAGTGGCACTACGCCAAAATACAGTAAATTACGATTCAGCTCTTCT
TTCGGTTACAGGTATTTGAC

Downstream 100 bases:

>100_bases
TTTTTATTTGAAATCGGTGTTTTTAACCTCATATTTAATATTAGGCTGACCCCGAGGTTTGGAGAAGCAACATGGCTTAT
AGCGAAAAGGTAATTGACCA

Product: tRNA 4-thiouridine sulfurtransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MKRPIYLDYAATTPVLPEVAERMMECLTFEGTFGNPASRSHAYGWQAEEKVEYAREQVANLIKADPREIVWTSGATESDN
LALKGIAQFYGSKGKHIITSKIEHKAVLDPCRELEEAGFEITYLEPEPRTGLITPEMVAAALRPDTILVSLMMVNNEIGT
ITDVAAIGELTRANKTFFHVDAAQAAGKVEIDLSTMKIDLMSFSAHKAYGPKGIGALYVRRTPRVRLKAQIHGGGHERGM
RSGTLATHQIVGMGEAFEIAGKTLHSEQQRLRKLRDKLWNGLQGLEQVFLNGHPTQNVANYLNVSFNFVEGESLMMALKD
AAVSSGSACTSATLEPSYVLRALGLSDELAHSSIRFSFGKYTTEEDIDHVLEVTKAAVEKLRELSPLWDMYKEGIDLSTV
EWAEH

Sequences:

>Translated_405_residues
MKRPIYLDYAATTPVLPEVAERMMECLTFEGTFGNPASRSHAYGWQAEEKVEYAREQVANLIKADPREIVWTSGATESDN
LALKGIAQFYGSKGKHIITSKIEHKAVLDPCRELEEAGFEITYLEPEPRTGLITPEMVAAALRPDTILVSLMMVNNEIGT
ITDVAAIGELTRANKTFFHVDAAQAAGKVEIDLSTMKIDLMSFSAHKAYGPKGIGALYVRRTPRVRLKAQIHGGGHERGM
RSGTLATHQIVGMGEAFEIAGKTLHSEQQRLRKLRDKLWNGLQGLEQVFLNGHPTQNVANYLNVSFNFVEGESLMMALKD
AAVSSGSACTSATLEPSYVLRALGLSDELAHSSIRFSFGKYTTEEDIDHVLEVTKAAVEKLRELSPLWDMYKEGIDLSTV
EWAEH
>Mature_405_residues
MKRPIYLDYAATTPVLPEVAERMMECLTFEGTFGNPASRSHAYGWQAEEKVEYAREQVANLIKADPREIVWTSGATESDN
LALKGIAQFYGSKGKHIITSKIEHKAVLDPCRELEEAGFEITYLEPEPRTGLITPEMVAAALRPDTILVSLMMVNNEIGT
ITDVAAIGELTRANKTFFHVDAAQAAGKVEIDLSTMKIDLMSFSAHKAYGPKGIGALYVRRTPRVRLKAQIHGGGHERGM
RSGTLATHQIVGMGEAFEIAGKTLHSEQQRLRKLRDKLWNGLQGLEQVFLNGHPTQNVANYLNVSFNFVEGESLMMALKD
AAVSSGSACTSATLEPSYVLRALGLSDELAHSSIRFSFGKYTTEEDIDHVLEVTKAAVEKLRELSPLWDMYKEGIDLSTV
EWAEH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=404, Percent_Identity=54.4554455445545, Blast_Score=472, Evalue=1e-133,
Organism=Homo sapiens, GI156713448, Length=422, Percent_Identity=32.7014218009479, Blast_Score=198, Evalue=7e-51,
Organism=Escherichia coli, GI48994898, Length=405, Percent_Identity=68.1481481481482, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI1789175, Length=396, Percent_Identity=26.2626262626263, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787970, Length=182, Percent_Identity=31.8681318681319, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25143064, Length=405, Percent_Identity=54.5679012345679, Blast_Score=456, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17533177, Length=325, Percent_Identity=33.2307692307692, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6319831, Length=403, Percent_Identity=54.3424317617866, Blast_Score=455, Evalue=1e-129,
Organism=Drosophila melanogaster, GI20129463, Length=404, Percent_Identity=54.2079207920792, Blast_Score=447, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 44744; Mature: 44744

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRPIYLDYAATTPVLPEVAERMMECLTFEGTFGNPASRSHAYGWQAEEKVEYAREQVAN
CCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LIKADPREIVWTSGATESDNLALKGIAQFYGSKGKHIITSKIEHKAVLDPCRELEEAGFE
HHHCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCE
ITYLEPEPRTGLITPEMVAAALRPDTILVSLMMVNNEIGTITDVAAIGELTRANKTFFHV
EEEECCCCCCCCCCHHHHHHHHCCCHHEEEEHHHCCCCCCHHHHHHHHHHHHCCCEEEEE
DAAQAAGKVEIDLSTMKIDLMSFSAHKAYGPKGIGALYVRRTPRVRLKAQIHGGGHERGM
EHHHCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCC
RSGTLATHQIVGMGEAFEIAGKTLHSEQQRLRKLRDKLWNGLQGLEQVFLNGHPTQNVAN
CCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
YLNVSFNFVEGESLMMALKDAAVSSGSACTSATLEPSYVLRALGLSDELAHSSIRFSFGK
HHCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHEEECCC
YTTEEDIDHVLEVTKAAVEKLRELSPLWDMYKEGIDLSTVEWAEH
CCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKRPIYLDYAATTPVLPEVAERMMECLTFEGTFGNPASRSHAYGWQAEEKVEYAREQVAN
CCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LIKADPREIVWTSGATESDNLALKGIAQFYGSKGKHIITSKIEHKAVLDPCRELEEAGFE
HHHCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCE
ITYLEPEPRTGLITPEMVAAALRPDTILVSLMMVNNEIGTITDVAAIGELTRANKTFFHV
EEEECCCCCCCCCCHHHHHHHHCCCHHEEEEHHHCCCCCCHHHHHHHHHHHHCCCEEEEE
DAAQAAGKVEIDLSTMKIDLMSFSAHKAYGPKGIGALYVRRTPRVRLKAQIHGGGHERGM
EHHHCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCC
RSGTLATHQIVGMGEAFEIAGKTLHSEQQRLRKLRDKLWNGLQGLEQVFLNGHPTQNVAN
CCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
YLNVSFNFVEGESLMMALKDAAVSSGSACTSATLEPSYVLRALGLSDELAHSSIRFSFGK
HHCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHEEECCC
YTTEEDIDHVLEVTKAAVEKLRELSPLWDMYKEGIDLSTVEWAEH
CCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA