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The map label for this gene is purF [H]

Identifier: 50084505

GI number: 50084505

Start: 1320632

End: 1322170

Strand: Direct

Name: purF [H]

Synonym: ACIAD1323

Alternate gene names: 50084505

Gene position: 1320632-1322170 (Clockwise)

Preceding gene: 50084504

Following gene: 50084506

Centisome position: 36.7

GC content: 42.3

Gene sequence:

>1539_bases
ATGTGTGGAGTAGTTGGTATAGCCGGTAAAGCGCCAGTTAACCAAATGTTGTTTGATGCATTAACGATGTTGCAGCATCG
GGGGCAAGATGCAGCTGGGATTGTAACCTGCCATGATGGCCGTTTATTTCTCAGAAAAGACAATGGAATGGTGCGTGATG
TATTCCATACTCGTCATATGCGTGCTTTGATCGGTAATTACGGTATTGGTCATGTGCGCTATCCAACTGCGGGTTCCTCA
AGTAGTGCTGAGGCACAACCTTTTTATGTGAATTCACCCTATGGAATTACGCTTGCACACAATGGTAATCTGACCAACGC
TGAAGAAATTCACGATGATCTGTTTAAAACAGATTTGCGTCATATGAATACAGATTCCGATTCCGAAGTTTTGCTTAACG
TGTTTGCGCACGAATTGCAAAAGAGCGGCAAGTTATCGCCTACATCAGAAGATGTATTCCATGCGGTTTCTCGTGTGCAT
GAGCGCTGCAAGGGTGCTTATGGCGTAGTTGCGATGATCACTGGACATGGTCTTGTTGGTTTCCGTGATCCAAATGGGAT
TCGACCATTAATTTATGGTTCGCGTGAAACTGAAAAGGGCGTTGAATATATTATTGCCTCTGAGTCTGTTGCAATTACAG
CACTCGGATTCAAAATTGAACGCGATATTCAGCCAGGCGAAGCAATTTTCATCAATGATCAGGGTGAATTGTTTACAAAG
CAATGTGCTACAAATCCACAGCATCGCCCATGTATCTTTGAATATGTTTATTTTGCGCGTCCAGATGCCATTATTGATGG
TATTTCTGTGTATAAAGCCCGTTTAAAGATGGGTGAGAAGCTGGCAAATAAAATCTTGCGCGAGTGGGGTGAAGATCACG
GAATTGATGTCGTAATTCCAATTCCAGATACAAGTCGTACATCAGCACTTGAACTGGCGAATATTCTAGGTGTGAAATTC
CGTGAAGGTTTTATGAAAAACCGTTATATCGGACGTACCTTTATTATGCCAGGGCAGCAACAACGTAAAAAATCGGTACG
TCAAAAGCTGAACCCAGTTGAGCTTGAATTTAAAGGTAAAAATGTATTGCTTGTAGACGATTCAATCGTTCGCGGTACCA
CCTGTAATGAAATTATTCAAATGGCGCGTGATTCTGGTGCTAAAAAGGTATATTTTGCCTCTGCTGCACCTATGGTGAAG
TATCCAAATGTCTATGGTATTGATATGCCTGCCAAGACAGAACTGATTGCTTCGCAACGTAGTGTTGAAGAGATTCGTGA
AATTATCGGTGCAGACCGTTTGGTTTTCCAAGACCTGGATGATCTAAAAAGTGCTGTACGTACCACCAAAGTACCTGATC
TGACAGAGTTTGATAGTTCTGTTTTTGATGGAGTGTATGTGGCAGGTGGGATTGATAAGGCTTATCTTGAAAACCTTGAG
AAGAAGCGCAACGATACTGCCAAAAATGCAGCGGATAGTTATATTGATGTGAATATCGATGCTGCTTCTGTGGATTTGAC
TGGAATTCGAGAGCTATAA

Upstream 100 bases:

>100_bases
ATCCTCAAATGAAGTGGCCGAATCTGATCATGAAAAAGATCATTCGGATCACTCGACGCAAAACCCTTTTTATTAATCCT
AGCTTGTCTGCGAGGTTGCT

Downstream 100 bases:

>100_bases
GATTTGTTGATGAAAAAGCGGGTTTTTCCCGCTTTTTTATTTTATGATTGGACAAAACAATATCACTAGGATTGTTTGAC
TTTGAGAGAAGTTCGTCATT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MCGVVGIAGKAPVNQMLFDALTMLQHRGQDAAGIVTCHDGRLFLRKDNGMVRDVFHTRHMRALIGNYGIGHVRYPTAGSS
SSAEAQPFYVNSPYGITLAHNGNLTNAEEIHDDLFKTDLRHMNTDSDSEVLLNVFAHELQKSGKLSPTSEDVFHAVSRVH
ERCKGAYGVVAMITGHGLVGFRDPNGIRPLIYGSRETEKGVEYIIASESVAITALGFKIERDIQPGEAIFINDQGELFTK
QCATNPQHRPCIFEYVYFARPDAIIDGISVYKARLKMGEKLANKILREWGEDHGIDVVIPIPDTSRTSALELANILGVKF
REGFMKNRYIGRTFIMPGQQQRKKSVRQKLNPVELEFKGKNVLLVDDSIVRGTTCNEIIQMARDSGAKKVYFASAAPMVK
YPNVYGIDMPAKTELIASQRSVEEIREIIGADRLVFQDLDDLKSAVRTTKVPDLTEFDSSVFDGVYVAGGIDKAYLENLE
KKRNDTAKNAADSYIDVNIDAASVDLTGIREL

Sequences:

>Translated_512_residues
MCGVVGIAGKAPVNQMLFDALTMLQHRGQDAAGIVTCHDGRLFLRKDNGMVRDVFHTRHMRALIGNYGIGHVRYPTAGSS
SSAEAQPFYVNSPYGITLAHNGNLTNAEEIHDDLFKTDLRHMNTDSDSEVLLNVFAHELQKSGKLSPTSEDVFHAVSRVH
ERCKGAYGVVAMITGHGLVGFRDPNGIRPLIYGSRETEKGVEYIIASESVAITALGFKIERDIQPGEAIFINDQGELFTK
QCATNPQHRPCIFEYVYFARPDAIIDGISVYKARLKMGEKLANKILREWGEDHGIDVVIPIPDTSRTSALELANILGVKF
REGFMKNRYIGRTFIMPGQQQRKKSVRQKLNPVELEFKGKNVLLVDDSIVRGTTCNEIIQMARDSGAKKVYFASAAPMVK
YPNVYGIDMPAKTELIASQRSVEEIREIIGADRLVFQDLDDLKSAVRTTKVPDLTEFDSSVFDGVYVAGGIDKAYLENLE
KKRNDTAKNAADSYIDVNIDAASVDLTGIREL
>Mature_512_residues
MCGVVGIAGKAPVNQMLFDALTMLQHRGQDAAGIVTCHDGRLFLRKDNGMVRDVFHTRHMRALIGNYGIGHVRYPTAGSS
SSAEAQPFYVNSPYGITLAHNGNLTNAEEIHDDLFKTDLRHMNTDSDSEVLLNVFAHELQKSGKLSPTSEDVFHAVSRVH
ERCKGAYGVVAMITGHGLVGFRDPNGIRPLIYGSRETEKGVEYIIASESVAITALGFKIERDIQPGEAIFINDQGELFTK
QCATNPQHRPCIFEYVYFARPDAIIDGISVYKARLKMGEKLANKILREWGEDHGIDVVIPIPDTSRTSALELANILGVKF
REGFMKNRYIGRTFIMPGQQQRKKSVRQKLNPVELEFKGKNVLLVDDSIVRGTTCNEIIQMARDSGAKKVYFASAAPMVK
YPNVYGIDMPAKTELIASQRSVEEIREIIGADRLVFQDLDDLKSAVRTTKVPDLTEFDSSVFDGVYVAGGIDKAYLENLE
KKRNDTAKNAADSYIDVNIDAASVDLTGIREL

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=478, Percent_Identity=34.9372384937238, Blast_Score=251, Evalue=8e-67,
Organism=Escherichia coli, GI1788651, Length=490, Percent_Identity=63.8775510204082, Blast_Score=642, Evalue=0.0,
Organism=Escherichia coli, GI1790167, Length=177, Percent_Identity=30.5084745762712, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=462, Percent_Identity=32.9004329004329, Blast_Score=231, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6323958, Length=507, Percent_Identity=53.8461538461538, Blast_Score=541, Evalue=1e-155,
Organism=Drosophila melanogaster, GI24659598, Length=481, Percent_Identity=33.0561330561331, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24659604, Length=478, Percent_Identity=33.0543933054393, Blast_Score=241, Evalue=8e-64,
Organism=Drosophila melanogaster, GI28573187, Length=474, Percent_Identity=32.4894514767932, Blast_Score=234, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 56735; Mature: 56735

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGVVGIAGKAPVNQMLFDALTMLQHRGQDAAGIVTCHDGRLFLRKDNGMVRDVFHTRHM
CCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEECCCCCHHHHHHHHHH
RALIGNYGIGHVRYPTAGSSSSAEAQPFYVNSPYGITLAHNGNLTNAEEIHDDLFKTDLR
HHHHCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHH
HMNTDSDSEVLLNVFAHELQKSGKLSPTSEDVFHAVSRVHERCKGAYGVVAMITGHGLVG
HCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEE
FRDPNGIRPLIYGSRETEKGVEYIIASESVAITALGFKIERDIQPGEAIFINDQGELFTK
ECCCCCCCCEEECCCCCCCCCEEEEECCCEEEEEEEEEEECCCCCCCEEEECCCCCHHHH
QCATNPQHRPCIFEYVYFARPDAIIDGISVYKARLKMGEKLANKILREWGEDHGIDVVIP
HHCCCCCCCCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
IPDTSRTSALELANILGVKFREGFMKNRYIGRTFIMPGQQQRKKSVRQKLNPVELEFKGK
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCEEEEECCC
NVLLVDDSIVRGTTCNEIIQMARDSGAKKVYFASAAPMVKYPNVYGIDMPAKTELIASQR
EEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCEECCCEEECCCCCHHHHHHHHH
SVEEIREIIGADRLVFQDLDDLKSAVRTTKVPDLTEFDSSVFDGVYVAGGIDKAYLENLE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEECCCCHHHHHHHH
KKRNDTAKNAADSYIDVNIDAASVDLTGIREL
HHCCCHHHHCCCCEEEEEEEEEEEECCCCCCC
>Mature Secondary Structure
MCGVVGIAGKAPVNQMLFDALTMLQHRGQDAAGIVTCHDGRLFLRKDNGMVRDVFHTRHM
CCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEECCCCCHHHHHHHHHH
RALIGNYGIGHVRYPTAGSSSSAEAQPFYVNSPYGITLAHNGNLTNAEEIHDDLFKTDLR
HHHHCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHH
HMNTDSDSEVLLNVFAHELQKSGKLSPTSEDVFHAVSRVHERCKGAYGVVAMITGHGLVG
HCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEE
FRDPNGIRPLIYGSRETEKGVEYIIASESVAITALGFKIERDIQPGEAIFINDQGELFTK
ECCCCCCCCEEECCCCCCCCCEEEEECCCEEEEEEEEEEECCCCCCCEEEECCCCCHHHH
QCATNPQHRPCIFEYVYFARPDAIIDGISVYKARLKMGEKLANKILREWGEDHGIDVVIP
HHCCCCCCCCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
IPDTSRTSALELANILGVKFREGFMKNRYIGRTFIMPGQQQRKKSVRQKLNPVELEFKGK
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCEEEEECCC
NVLLVDDSIVRGTTCNEIIQMARDSGAKKVYFASAAPMVKYPNVYGIDMPAKTELIASQR
EEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCEECCCEEECCCCCHHHHHHHHH
SVEEIREIIGADRLVFQDLDDLKSAVRTTKVPDLTEFDSSVFDGVYVAGGIDKAYLENLE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEECCCCHHHHHHHH
KKRNDTAKNAADSYIDVNIDAASVDLTGIREL
HHCCCHHHHCCCCEEEEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]