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The map label for this gene is trmB

Identifier: 50084485

GI number: 50084485

Start: 1304887

End: 1305624

Strand: Reverse

Name: trmB

Synonym: ACIAD1302

Alternate gene names: 50084485

Gene position: 1305624-1304887 (Counterclockwise)

Preceding gene: 50084487

Following gene: 50084477

Centisome position: 36.28

GC content: 42.55

Gene sequence:

>738_bases
TTGAGTCATACCAAAATTATGTCGACTGATCAGTTAGAAACGCAGATTACTGAACTGGATAATTTGCCTGAACACCGCGA
AATTGTGACATTTATGCGTCGCTCTGCGCCGCTTAATACCTCGCAGCGTTCCGCTTTAGAGCAATATCAAGATTTAATTT
TGGAATATCCCGTTGGTGATTTACGCCAGCACTTTGAACACCCTGAACACCCATTAACGGTTGAAATCGGTTTTGGTATG
GGCCGTTCTTTAGTTTTGATGGCCAAAGCTCATCCTGAACGTAATTTTGTAGGAATTGAAGTACATGTTCCTGGAATTGC
ACAATGTGTTTATGAAGCAGGCATGGCTGGCCTAAAAAATTTACGTGTACTGGATGCCGACGCTATTCAGGTACTTCGTG
AAATGCCAGACAACAGCATCAACTGCGTACAACTTTATTTTCCAGATCCGTGGCAGAAAAAACGTCATTTTAAACGCCGT
TTCGTTATTCATGAACGTATGCAACTGGTCGAGCAAAAACTAGAACTTGGCGGAACTTTTCACGCAGCGACCGACTGGGA
ACCTTATGCAGAATGGATGCTTGATGTCCTAGATAATCGCCCTGCTTTAGAAAACCTGGCAGGTAAAGGCAATAGTTACC
CTCGCCCAGAATGGCGTCCACAAACTAAATTCGAACGTCGTGGTATCGAATCTGGTCACAAGATCAATGATTTTATTTTT
AAGAAAATTGGATCATAA

Upstream 100 bases:

>100_bases
AGTTGATCTATATGACTGCATCAGAAAAGATGGTCTAAATGATTATTCAGAATAGGTTTGACATCATTTCTCAAGTATTT
ACAATACGGCATCTGGATTT

Downstream 100 bases:

>100_bases
GCTGATTAACACCTAGCTGATACCATATTTAATCGCATTAAATATTAACTGTTTAATGCGATTTTTATTTTTTTACAGCG
TGGTGATTTTCTACGCTTAA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MSHTKIMSTDQLETQITELDNLPEHREIVTFMRRSAPLNTSQRSALEQYQDLILEYPVGDLRQHFEHPEHPLTVEIGFGM
GRSLVLMAKAHPERNFVGIEVHVPGIAQCVYEAGMAGLKNLRVLDADAIQVLREMPDNSINCVQLYFPDPWQKKRHFKRR
FVIHERMQLVEQKLELGGTFHAATDWEPYAEWMLDVLDNRPALENLAGKGNSYPRPEWRPQTKFERRGIESGHKINDFIF
KKIGS

Sequences:

>Translated_245_residues
MSHTKIMSTDQLETQITELDNLPEHREIVTFMRRSAPLNTSQRSALEQYQDLILEYPVGDLRQHFEHPEHPLTVEIGFGM
GRSLVLMAKAHPERNFVGIEVHVPGIAQCVYEAGMAGLKNLRVLDADAIQVLREMPDNSINCVQLYFPDPWQKKRHFKRR
FVIHERMQLVEQKLELGGTFHAATDWEPYAEWMLDVLDNRPALENLAGKGNSYPRPEWRPQTKFERRGIESGHKINDFIF
KKIGS
>Mature_244_residues
SHTKIMSTDQLETQITELDNLPEHREIVTFMRRSAPLNTSQRSALEQYQDLILEYPVGDLRQHFEHPEHPLTVEIGFGMG
RSLVLMAKAHPERNFVGIEVHVPGIAQCVYEAGMAGLKNLRVLDADAIQVLREMPDNSINCVQLYFPDPWQKKRHFKRRF
VIHERMQLVEQKLELGGTFHAATDWEPYAEWMLDVLDNRPALENLAGKGNSYPRPEWRPQTKFERRGIESGHKINDFIFK
KIGS

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=227, Percent_Identity=48.4581497797357, Blast_Score=205, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_ACIAD (Q6FCN6)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045995.1
- ProteinModelPortal:   Q6FCN6
- SMR:   Q6FCN6
- STRING:   Q6FCN6
- GeneID:   2880729
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1302
- NMPDR:   fig|62977.3.peg.477
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   RERANAK
- PhylomeDB:   Q6FCN6
- ProtClustDB:   PRK00121
- BioCyc:   ASP62977:ACIAD1302-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 28399; Mature: 28268

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: BINDING 75-75 BINDING 100-100 BINDING 127-127 BINDING 149-149 BINDING 153-153 BINDING 185-185

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHTKIMSTDQLETQITELDNLPEHREIVTFMRRSAPLNTSQRSALEQYQDLILEYPVGD
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHH
LRQHFEHPEHPLTVEIGFGMGRSLVLMAKAHPERNFVGIEVHVPGIAQCVYEAGMAGLKN
HHHHHCCCCCCEEEEECCCCCCEEEEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHC
LRVLDADAIQVLREMPDNSINCVQLYFPDPWQKKRHFKRRFVIHERMQLVEQKLELGGTF
CEEECHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
HAATDWEPYAEWMLDVLDNRPALENLAGKGNSYPRPEWRPQTKFERRGIESGHKINDFIF
ECCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHH
KKIGS
HHHCC
>Mature Secondary Structure 
SHTKIMSTDQLETQITELDNLPEHREIVTFMRRSAPLNTSQRSALEQYQDLILEYPVGD
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHH
LRQHFEHPEHPLTVEIGFGMGRSLVLMAKAHPERNFVGIEVHVPGIAQCVYEAGMAGLKN
HHHHHCCCCCCEEEEECCCCCCEEEEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHC
LRVLDADAIQVLREMPDNSINCVQLYFPDPWQKKRHFKRRFVIHERMQLVEQKLELGGTF
CEEECHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
HAATDWEPYAEWMLDVLDNRPALENLAGKGNSYPRPEWRPQTKFERRGIESGHKINDFIF
ECCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHH
KKIGS
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA