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The map label for this gene is purA

Identifier: 50084444

GI number: 50084444

Start: 1256743

End: 1258062

Strand: Direct

Name: purA

Synonym: ACIAD1258

Alternate gene names: 50084444

Gene position: 1256743-1258062 (Clockwise)

Preceding gene: 50084443

Following gene: 50084445

Centisome position: 34.92

GC content: 44.62

Gene sequence:

>1320_bases
ATGGGCAAAAATGTTGTGGTACTTGGTACCCAATGGGGCGACGAAGGTAAAGGTAAGATCGTCGACCTGCTCACAGATCA
GGCGGCTGCCGTTGTACGTTATCAAGGCGGGCACAATGCAGGGCATACTCTTGTCGTAGGTGGAAAAAAGACTGTTTTAC
ACCTCATTCCATCAGGCATTTTACGTGAAAACGTGTTGTGCTTAATTGGAAATGGCGTTGTACTTTCACCTGCTGCATTA
ATTAAAGAAATGGGTATTTTGGAAGAAGAAGGCGTGCCTGTGAAGGAACGTTTACGTATCTCTCCAAACTGTCCACTTAT
TTTGCCAAACCACATTGCACTTGACCAAGCGCGTGAGAAAAAACGTGGCAATGCGAAAATTGGTACAACAGGTCGTGGTA
TTGGCCCAGCGTACGAAGATAAAGTTGCTCGTCGTGCAGTACGTGTAGCCGATTTGGTTCGTGGCGGTGCTGCTTTAGAA
GAAAAACTTCAAGAGATGCTTGAACTACACAACTTCCAGCTCACTCAATTCTACGGTGTAGAAGCAGTGAAGTTTGAAGA
TGTACTTGCTCTATGTAATGAGTGGCGCGAAGTATTGGCACCATTGGTGATTGATGTGACCAAAGTTCTACATGATTACC
GTAAAGAAGGTAAAGCAATCATGTTTGAAGGTGCGCAAGGTTCATTACTTGACATCGATCATGGTACTTATCCGTATGTG
ACCAGTTCAAATACTACAGCGGGTGGCGTAAGCTCTGGCTCGGGTATGGGACCATTGCATTTAGATTATGTTTTGGGGAT
TACTAAAGCCTATACCACACGTGTTGGTGCGGGTCCATTCCCAACTGAATTGCATTATGATGCTGCCACTGATACAGGTG
ATGCGATTGGCCGTCATTTGGGTACAGTCGGCCATGAGTTTGGTGCATCTACGGGTCGTCAACGTCGTTGTGGCTGGTTC
GATGCAGAGATCTTGCGTCGTTCAGTTGAAGTGAATTCGTTATCTGGTATCTGCCTAACCAAACTTGATGTTCTGGATGG
TTTGGACGAGATTAAGATTTGTGTAGGCTATGAAGATGTTGATTCAGGCTGTGCAGGTTCTTCTGATGCGGTTTCATTTG
AAAGCTTAAAACCAATTTATGAAACTATGCCAGGCTGGAGTGAATCAACGGTTGGTTTGACCAGCATTGATCAGCTTCCT
GCAAATGCTTTGGCTTATGTGAAACGCATTGAACAATTGATCGAATGCCCAATTGATATTATTTCAACGGGTCCAGATCG
TGCTGAAACAATGATATTGCGTCATCCATTTTCTGCTTAA

Upstream 100 bases:

>100_bases
TAGCACAAGATCAGTGGCAAATTAACAAGATTTAATGGTTTGTTTGTTCAATAAAGCAAACAAATGTTCACTTTTAACAG
CATGATGTAATGAGGCTATT

Downstream 100 bases:

>100_bases
GATCTTGTATTGAAATACGACTAAAGAGAAGCTCCTGATCAGGAGCTTTTCTTTTTTAGATAGGTACAATAAAAAATAAT
CAATATTTCATAATGAGAAA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MGKNVVVLGTQWGDEGKGKIVDLLTDQAAAVVRYQGGHNAGHTLVVGGKKTVLHLIPSGILRENVLCLIGNGVVLSPAAL
IKEMGILEEEGVPVKERLRISPNCPLILPNHIALDQAREKKRGNAKIGTTGRGIGPAYEDKVARRAVRVADLVRGGAALE
EKLQEMLELHNFQLTQFYGVEAVKFEDVLALCNEWREVLAPLVIDVTKVLHDYRKEGKAIMFEGAQGSLLDIDHGTYPYV
TSSNTTAGGVSSGSGMGPLHLDYVLGITKAYTTRVGAGPFPTELHYDAATDTGDAIGRHLGTVGHEFGASTGRQRRCGWF
DAEILRRSVEVNSLSGICLTKLDVLDGLDEIKICVGYEDVDSGCAGSSDAVSFESLKPIYETMPGWSESTVGLTSIDQLP
ANALAYVKRIEQLIECPIDIISTGPDRAETMILRHPFSA

Sequences:

>Translated_439_residues
MGKNVVVLGTQWGDEGKGKIVDLLTDQAAAVVRYQGGHNAGHTLVVGGKKTVLHLIPSGILRENVLCLIGNGVVLSPAAL
IKEMGILEEEGVPVKERLRISPNCPLILPNHIALDQAREKKRGNAKIGTTGRGIGPAYEDKVARRAVRVADLVRGGAALE
EKLQEMLELHNFQLTQFYGVEAVKFEDVLALCNEWREVLAPLVIDVTKVLHDYRKEGKAIMFEGAQGSLLDIDHGTYPYV
TSSNTTAGGVSSGSGMGPLHLDYVLGITKAYTTRVGAGPFPTELHYDAATDTGDAIGRHLGTVGHEFGASTGRQRRCGWF
DAEILRRSVEVNSLSGICLTKLDVLDGLDEIKICVGYEDVDSGCAGSSDAVSFESLKPIYETMPGWSESTVGLTSIDQLP
ANALAYVKRIEQLIECPIDIISTGPDRAETMILRHPFSA
>Mature_438_residues
GKNVVVLGTQWGDEGKGKIVDLLTDQAAAVVRYQGGHNAGHTLVVGGKKTVLHLIPSGILRENVLCLIGNGVVLSPAALI
KEMGILEEEGVPVKERLRISPNCPLILPNHIALDQAREKKRGNAKIGTTGRGIGPAYEDKVARRAVRVADLVRGGAALEE
KLQEMLELHNFQLTQFYGVEAVKFEDVLALCNEWREVLAPLVIDVTKVLHDYRKEGKAIMFEGAQGSLLDIDHGTYPYVT
SSNTTAGGVSSGSGMGPLHLDYVLGITKAYTTRVGAGPFPTELHYDAATDTGDAIGRHLGTVGHEFGASTGRQRRCGWFD
AEILRRSVEVNSLSGICLTKLDVLDGLDEIKICVGYEDVDSGCAGSSDAVSFESLKPIYETMPGWSESTVGLTSIDQLPA
NALAYVKRIEQLIECPIDIISTGPDRAETMILRHPFSA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI34577063, Length=435, Percent_Identity=40.2298850574713, Blast_Score=291, Evalue=8e-79,
Organism=Homo sapiens, GI22748717, Length=435, Percent_Identity=40.6896551724138, Blast_Score=288, Evalue=5e-78,
Organism=Homo sapiens, GI40316944, Length=406, Percent_Identity=38.6699507389163, Blast_Score=248, Evalue=6e-66,
Organism=Escherichia coli, GI1790620, Length=441, Percent_Identity=64.172335600907, Blast_Score=563, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI25146553, Length=439, Percent_Identity=43.2801822323462, Blast_Score=327, Evalue=9e-90,
Organism=Caenorhabditis elegans, GI25146555, Length=439, Percent_Identity=43.2801822323462, Blast_Score=326, Evalue=1e-89,
Organism=Saccharomyces cerevisiae, GI6324109, Length=443, Percent_Identity=41.3092550790068, Blast_Score=313, Evalue=4e-86,
Organism=Drosophila melanogaster, GI21356033, Length=433, Percent_Identity=40.4157043879908, Blast_Score=305, Evalue=4e-83,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_ACIAD (Q6FCS7)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045954.1
- ProteinModelPortal:   Q6FCS7
- SMR:   Q6FCS7
- STRING:   Q6FCS7
- GeneID:   2879345
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1258
- NMPDR:   fig|62977.3.peg.327
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- PhylomeDB:   Q6FCS7
- ProtClustDB:   PRK01117
- BioCyc:   ASP62977:ACIAD1258-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47239; Mature: 47107

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2; PS00041 HTH_ARAC_FAMILY_1

Important sites: ACT_SITE 14-14 ACT_SITE 42-42 BINDING 130-130 BINDING 144-144 BINDING 226-226 BINDING 241-241 BINDING 313-313 BINDING 315-315

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKNVVVLGTQWGDEGKGKIVDLLTDQAAAVVRYQGGHNAGHTLVVGGKKTVLHLIPSGI
CCCCEEEEEECCCCCCCCCEEEEHHCCCEEEEEECCCCCCCCEEEECCCHHEEHHHCCHH
LRENVLCLIGNGVVLSPAALIKEMGILEEEGVPVKERLRISPNCPLILPNHIALDQAREK
HHCCCEEEECCCEEECHHHHHHHHCCCCCCCCCHHHHEEECCCCCEEECCCHHHHHHHHH
KRGNAKIGTTGRGIGPAYEDKVARRAVRVADLVRGGAALEEKLQEMLELHNFQLTQFYGV
HCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEECC
EAVKFEDVLALCNEWREVLAPLVIDVTKVLHDYRKEGKAIMFEGAQGSLLDIDHGTYPYV
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEE
TSSNTTAGGVSSGSGMGPLHLDYVLGITKAYTTRVGAGPFPTELHYDAATDTGDAIGRHL
ECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHH
GTVGHEFGASTGRQRRCGWFDAEILRRSVEVNSLSGICLTKLDVLDGLDEIKICVGYEDV
HHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCC
DSGCAGSSDAVSFESLKPIYETMPGWSESTVGLTSIDQLPANALAYVKRIEQLIECPIDI
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCHHE
ISTGPDRAETMILRHPFSA
ECCCCCHHHEEEEECCCCC
>Mature Secondary Structure 
GKNVVVLGTQWGDEGKGKIVDLLTDQAAAVVRYQGGHNAGHTLVVGGKKTVLHLIPSGI
CCCEEEEEECCCCCCCCCEEEEHHCCCEEEEEECCCCCCCCEEEECCCHHEEHHHCCHH
LRENVLCLIGNGVVLSPAALIKEMGILEEEGVPVKERLRISPNCPLILPNHIALDQAREK
HHCCCEEEECCCEEECHHHHHHHHCCCCCCCCCHHHHEEECCCCCEEECCCHHHHHHHHH
KRGNAKIGTTGRGIGPAYEDKVARRAVRVADLVRGGAALEEKLQEMLELHNFQLTQFYGV
HCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEECC
EAVKFEDVLALCNEWREVLAPLVIDVTKVLHDYRKEGKAIMFEGAQGSLLDIDHGTYPYV
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEE
TSSNTTAGGVSSGSGMGPLHLDYVLGITKAYTTRVGAGPFPTELHYDAATDTGDAIGRHL
ECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHH
GTVGHEFGASTGRQRRCGWFDAEILRRSVEVNSLSGICLTKLDVLDGLDEIKICVGYEDV
HHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCC
DSGCAGSSDAVSFESLKPIYETMPGWSESTVGLTSIDQLPANALAYVKRIEQLIECPIDI
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCHHE
ISTGPDRAETMILRHPFSA
ECCCCCHHHEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA