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The map label for this gene is insF1 [H]

Identifier: 50084435

GI number: 50084435

Start: 1248198

End: 1249049

Strand: Reverse

Name: insF1 [H]

Synonym: ACIAD1249

Alternate gene names: 50084435

Gene position: 1249049-1248198 (Counterclockwise)

Preceding gene: 50084436

Following gene: 50084432

Centisome position: 34.71

GC content: 43.19

Gene sequence:

>852_bases
GTGAATGAAGTCACTGTATCTTCTGCCTGTAAATGCCTGGGTGTCAGCACTTCAGGCTATTATGCCTGGCGAAAACGCCA
GACCAATCTAGCGCAGAAATATAATGATTTAAAAGCCGTGTATTGGCAGCATCATGCACGATTGGGTGCACCTTCATTGG
TACATGACATGCATGATTTAGGTTACAGCATGAGCGAACGAACCGTTGGAAGAATGCTTAAAAAACTTGGTTTACGGAGC
AAAATTGCACGTAAATACAAGCATACGACTGATTCAAACCATCGTTTGCCTACAGCGCCTAACTTGTTGGATCGTCAATT
TACGGTCAATGAACCCAATAAGATCTGGACAACGGATATTACCTATATCCGTACTAAGCAAGGTTGGCTGTATTTATGTG
TGATGCTGGATCTATTCAGCCGTCGTATTGTCGGTTGGCAAACCAGCCATCGAATAGACCGTCAATTGGTGTGTGATGCG
TTTCATTATGCAATGGCTCGTCAGGGGTATCCAATGGGTGTCATGGTACATTCGGACCAAGGCTCACAGTACTGTAGTCG
TGATTTTAGGGCGCTATTATTGACGAATAACTGCGTTCAAAGTATGTCACGCAGGGGAAACTGTTGGGATAATGCAGTGA
CCGAAAGCTTCTTTCATACATTGAAAGGTCATATGGTCCATGGCAGTGTGTTTGCCACTCGAAAAGAAGCGAATGCTGTC
TTGTTTGACTATATTGAGATTTATTACAATCGAATCAGACGACATTCTACAAACGGCTGGTTAAGCCCAGAAGCCTTTGA
ACAGAAATATTTCAAGAATTTAGAGGGATTTGTTGTCCACGATACTGTCTAG

Upstream 100 bases:

>100_bases
TCAAACAGCTCAGGGAAGCCAATGACATCTTAAAAAAAGCGCATGTGTACTTTCTGACAGATCATGCCAAGAAAAGTACA
CGGTAATTCAAGATCTGGAT

Downstream 100 bases:

>100_bases
GATCAATGAACATCTTCAACATTTAGATCAAGATTATCAGCCTTCTTTTGAAGATGATAACTACTCACAAAATAATTTTA
ACCGTTAAAGTTATTTTTTA

Product: IS1236 transposase protein 2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MNEVTVSSACKCLGVSTSGYYAWRKRQTNLAQKYNDLKAVYWQHHARLGAPSLVHDMHDLGYSMSERTVGRMLKKLGLRS
KIARKYKHTTDSNHRLPTAPNLLDRQFTVNEPNKIWTTDITYIRTKQGWLYLCVMLDLFSRRIVGWQTSHRIDRQLVCDA
FHYAMARQGYPMGVMVHSDQGSQYCSRDFRALLLTNNCVQSMSRRGNCWDNAVTESFFHTLKGHMVHGSVFATRKEANAV
LFDYIEIYYNRIRRHSTNGWLSPEAFEQKYFKNLEGFVVHDTV

Sequences:

>Translated_283_residues
MNEVTVSSACKCLGVSTSGYYAWRKRQTNLAQKYNDLKAVYWQHHARLGAPSLVHDMHDLGYSMSERTVGRMLKKLGLRS
KIARKYKHTTDSNHRLPTAPNLLDRQFTVNEPNKIWTTDITYIRTKQGWLYLCVMLDLFSRRIVGWQTSHRIDRQLVCDA
FHYAMARQGYPMGVMVHSDQGSQYCSRDFRALLLTNNCVQSMSRRGNCWDNAVTESFFHTLKGHMVHGSVFATRKEANAV
LFDYIEIYYNRIRRHSTNGWLSPEAFEQKYFKNLEGFVVHDTV
>Mature_283_residues
MNEVTVSSACKCLGVSTSGYYAWRKRQTNLAQKYNDLKAVYWQHHARLGAPSLVHDMHDLGYSMSERTVGRMLKKLGLRS
KIARKYKHTTDSNHRLPTAPNLLDRQFTVNEPNKIWTTDITYIRTKQGWLYLCVMLDLFSRRIVGWQTSHRIDRQLVCDA
FHYAMARQGYPMGVMVHSDQGSQYCSRDFRALLLTNNCVQSMSRRGNCWDNAVTESFFHTLKGHMVHGSVFATRKEANAV
LFDYIEIYYNRIRRHSTNGWLSPEAFEQKYFKNLEGFVVHDTV

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=232, Percent_Identity=30.6034482758621, Blast_Score=125, Evalue=3e-29,
Organism=Escherichia coli, GI1788405, Length=274, Percent_Identity=43.0656934306569, Blast_Score=237, Evalue=6e-64,
Organism=Escherichia coli, GI1787263, Length=274, Percent_Identity=43.0656934306569, Blast_Score=237, Evalue=6e-64,
Organism=Escherichia coli, GI1786753, Length=274, Percent_Identity=43.0656934306569, Blast_Score=237, Evalue=6e-64,
Organism=Escherichia coli, GI1786570, Length=274, Percent_Identity=43.0656934306569, Blast_Score=237, Evalue=6e-64,
Organism=Escherichia coli, GI1786490, Length=274, Percent_Identity=43.0656934306569, Blast_Score=237, Evalue=6e-64,
Organism=Escherichia coli, GI1789981, Length=223, Percent_Identity=32.2869955156951, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32963; Mature: 32963

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEVTVSSACKCLGVSTSGYYAWRKRQTNLAQKYNDLKAVYWQHHARLGAPSLVHDMHDL
CCCCCHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
GYSMSERTVGRMLKKLGLRSKIARKYKHTTDSNHRLPTAPNLLDRQFTVNEPNKIWTTDI
CCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCHHCCCEECCCCCEEEEECE
TYIRTKQGWLYLCVMLDLFSRRIVGWQTSHRIDRQLVCDAFHYAMARQGYPMGVMVHSDQ
EEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
GSQYCSRDFRALLLTNNCVQSMSRRGNCWDNAVTESFFHTLKGHMVHGSVFATRKEANAV
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEECHHEEEHHHCCHH
LFDYIEIYYNRIRRHSTNGWLSPEAFEQKYFKNLEGFVVHDTV
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEECCC
>Mature Secondary Structure
MNEVTVSSACKCLGVSTSGYYAWRKRQTNLAQKYNDLKAVYWQHHARLGAPSLVHDMHDL
CCCCCHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
GYSMSERTVGRMLKKLGLRSKIARKYKHTTDSNHRLPTAPNLLDRQFTVNEPNKIWTTDI
CCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCHHCCCEECCCCCEEEEECE
TYIRTKQGWLYLCVMLDLFSRRIVGWQTSHRIDRQLVCDAFHYAMARQGYPMGVMVHSDQ
EEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
GSQYCSRDFRALLLTNNCVQSMSRRGNCWDNAVTESFFHTLKGHMVHGSVFATRKEANAV
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEECHHEEEHHHCCHH
LFDYIEIYYNRIRRHSTNGWLSPEAFEQKYFKNLEGFVVHDTV
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]