Click here to switch to the map view.

The map label for this gene is ahpF [H]

Identifier: 50084421

GI number: 50084421

Start: 1234047

End: 1235606

Strand: Reverse

Name: ahpF [H]

Synonym: ACIAD1234

Alternate gene names: 50084421

Gene position: 1235606-1234047 (Counterclockwise)

Preceding gene: 50084423

Following gene: 50084420

Centisome position: 34.34

GC content: 45.06

Gene sequence:

>1560_bases
ATGTTAGACCAAAATATCAAAACTCAACTTAAAGCTTATTTAGAACGTTTGGAAAGCCCAATTGAATTGGTCGCTGCACT
CGATGACTCAGACAAAGCTGCCAAAATCAAAGAACTGGTCACTGAAATTGCAGAACTCTCCAACCAGGTGACAGCACGTT
TTGATGGTCAGAATACACGCCGTCCAAGTTTCGGTATTGCCAAAGCCGGTGAACAACCTCGTGTGTTCTTTGCAGGCTTG
CCGATGGGTCATGAGTTTACCTCACTCATTCTGGCATTGTTACAAACCTCAGGTTATGCACCTAAAGTGTCTGATGAAAT
CTTAAACCAGATTAAAGGCCTTAACTTAAAAGCTGATTTTGATGTGTTTGTTTCTTTAAGCTGTCATAACTGTCCAGATG
TGGTCCAGGCGTTAAACTTGATTGCGATCTATAATCCAAACAGTACTGCGACCATGATTGATGGGGCTTTCTTTCAGGAT
GAAGTTGAAGAACGCAAAATCATGGCCGTACCAATGGTGTTCCAGAATGGCAATCACATCGGTCAGGGTCGTATGACGCT
AGAGGAAATTGTCGCGAAACTGGACAGCAATGCTGCTGAGAAAGATGCCGCTGCCATTAATGCAAAAGATGTCTTTGATG
TATTGGTCGTAGGTGCTGGTCCTGCTGGTGGTACTGCCGCGATCTATGCAGCACGTAAAGGTATTCGTACTGGTATGATT
GCCGAGCGCTTTGGTGGTCAGGTGATGGACACCATGGACATTGAAAATTTCACCTCTGTACAAAAAACGGTGGGGCCAAA
GTTTGCTGCGGATATGGAAGCACATGTTCGTGAATATGATGTTGATATCATGAATTTGCAACGTGTCAGTAAGATTGTGG
GTGCAGATCAAACTGCTTCAGGTCTGGTTGAAGTTGAACTGGAAAATGGGGCTAAGGTTGAATCGAAAACCGTGATTTTA
TCGACGGGTGCGCGCTGGAGAAAAATGGGGGTTCCTGGTGAGCAGGAATATGCCACTCGTGGTGTCGCATATTGCCCACA
CTGTGATGGTCCATTGTTTAAAGGCAAGAAAGTTGCGGTGATTGGTGGAGGTAACTCGGGTGTCGAAGCTGCAATTGATT
TAGCAGGAATTGTTGAGCATGTAACGCTGGTGGAATTTGATACCAAACTTCGTGCAGACCAGGTATTGCAGGACAAGCTA
AATAGTTTGCCAAATGCGACCATTATTACCAATGCACTTTCTACAGAGGTGATCGGTGATGGTGCTCAAGTGACGGGTTT
GAAGTATAAAGACCGTGCGACAGATACAGAACACACGATTGAGCTGGCAGGGATTTTTGTACAGATTGGCTTGTTACCAA
ACACTGACTTTCTTAAAGGTTCGGTAGAGTTAAGCCCACGAGGTGAGATCATCATCAATGAGCGCAATGAAACCAATGTG
AAGGGTGTGTTTGCAGCAGGTGACTGTACCACAGTGCCGTACAAGCAGATCATCATTGCCACAGGTGAAGGTGCCAAAGC
GTCTCTGTCTGCTTTTGATTATATTATTCGTCATGGCTGA

Upstream 100 bases:

>100_bases
AAAACCGATTAAAACAATAAAAACATACTGTTTCACCTATTCTAACTTTTATCGTATGATCCTCATCAAGTTAAAGAATT
TACTCCTTTGGAGCTTCAAC

Downstream 100 bases:

>100_bases
TTAATCTCTATTTATAAGCTCACTTTTTATAAGGCTTATCTCCCTCGATAGATAAGCCTTTTTTTTTATTGCATTTTTTA
ACATTTTATTTACAAATATA

Product: alkyl hydroperoxide reductase subunit F

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MLDQNIKTQLKAYLERLESPIELVAALDDSDKAAKIKELVTEIAELSNQVTARFDGQNTRRPSFGIAKAGEQPRVFFAGL
PMGHEFTSLILALLQTSGYAPKVSDEILNQIKGLNLKADFDVFVSLSCHNCPDVVQALNLIAIYNPNSTATMIDGAFFQD
EVEERKIMAVPMVFQNGNHIGQGRMTLEEIVAKLDSNAAEKDAAAINAKDVFDVLVVGAGPAGGTAAIYAARKGIRTGMI
AERFGGQVMDTMDIENFTSVQKTVGPKFAADMEAHVREYDVDIMNLQRVSKIVGADQTASGLVEVELENGAKVESKTVIL
STGARWRKMGVPGEQEYATRGVAYCPHCDGPLFKGKKVAVIGGGNSGVEAAIDLAGIVEHVTLVEFDTKLRADQVLQDKL
NSLPNATIITNALSTEVIGDGAQVTGLKYKDRATDTEHTIELAGIFVQIGLLPNTDFLKGSVELSPRGEIIINERNETNV
KGVFAAGDCTTVPYKQIIIATGEGAKASLSAFDYIIRHG

Sequences:

>Translated_519_residues
MLDQNIKTQLKAYLERLESPIELVAALDDSDKAAKIKELVTEIAELSNQVTARFDGQNTRRPSFGIAKAGEQPRVFFAGL
PMGHEFTSLILALLQTSGYAPKVSDEILNQIKGLNLKADFDVFVSLSCHNCPDVVQALNLIAIYNPNSTATMIDGAFFQD
EVEERKIMAVPMVFQNGNHIGQGRMTLEEIVAKLDSNAAEKDAAAINAKDVFDVLVVGAGPAGGTAAIYAARKGIRTGMI
AERFGGQVMDTMDIENFTSVQKTVGPKFAADMEAHVREYDVDIMNLQRVSKIVGADQTASGLVEVELENGAKVESKTVIL
STGARWRKMGVPGEQEYATRGVAYCPHCDGPLFKGKKVAVIGGGNSGVEAAIDLAGIVEHVTLVEFDTKLRADQVLQDKL
NSLPNATIITNALSTEVIGDGAQVTGLKYKDRATDTEHTIELAGIFVQIGLLPNTDFLKGSVELSPRGEIIINERNETNV
KGVFAAGDCTTVPYKQIIIATGEGAKASLSAFDYIIRHG
>Mature_519_residues
MLDQNIKTQLKAYLERLESPIELVAALDDSDKAAKIKELVTEIAELSNQVTARFDGQNTRRPSFGIAKAGEQPRVFFAGL
PMGHEFTSLILALLQTSGYAPKVSDEILNQIKGLNLKADFDVFVSLSCHNCPDVVQALNLIAIYNPNSTATMIDGAFFQD
EVEERKIMAVPMVFQNGNHIGQGRMTLEEIVAKLDSNAAEKDAAAINAKDVFDVLVVGAGPAGGTAAIYAARKGIRTGMI
AERFGGQVMDTMDIENFTSVQKTVGPKFAADMEAHVREYDVDIMNLQRVSKIVGADQTASGLVEVELENGAKVESKTVIL
STGARWRKMGVPGEQEYATRGVAYCPHCDGPLFKGKKVAVIGGGNSGVEAAIDLAGIVEHVTLVEFDTKLRADQVLQDKL
NSLPNATIITNALSTEVIGDGAQVTGLKYKDRATDTEHTIELAGIFVQIGLLPNTDFLKGSVELSPRGEIIINERNETNV
KGVFAAGDCTTVPYKQIIIATGEGAKASLSAFDYIIRHG

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=518, Percent_Identity=61.969111969112, Blast_Score=645, Evalue=0.0,
Organism=Escherichia coli, GI1787114, Length=316, Percent_Identity=35.126582278481, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6321898, Length=339, Percent_Identity=31.5634218289086, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6320560, Length=312, Percent_Identity=32.6923076923077, Blast_Score=148, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 55804; Mature: 55804

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDQNIKTQLKAYLERLESPIELVAALDDSDKAAKIKELVTEIAELSNQVTARFDGQNTR
CCCCHHHHHHHHHHHHHCCHHHHHEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCC
RPSFGIAKAGEQPRVFFAGLPMGHEFTSLILALLQTSGYAPKVSDEILNQIKGLNLKADF
CCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEECC
DVFVSLSCHNCPDVVQALNLIAIYNPNSTATMIDGAFFQDEVEERKIMAVPMVFQNGNHI
EEEEEEECCCCHHHHHHCCEEEEECCCCCEEEECCHHHHHHHHCCEEEEEEEEEECCCEE
GQGRMTLEEIVAKLDSNAAEKDAAAINAKDVFDVLVVGAGPAGGTAAIYAARKGIRTGMI
CCCCHHHHHHHHHHCCCCCHHHHHHCCHHHHEEEEEEECCCCCCHHHHHHHHHCCHHCHH
AERFGGQVMDTMDIENFTSVQKTVGPKFAADMEAHVREYDVDIMNLQRVSKIVGADQTAS
HHHHCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCC
GLVEVELENGAKVESKTVILSTGARWRKMGVPGEQEYATRGVAYCPHCDGPLFKGKKVAV
CEEEEEECCCCEEECCEEEEECCCCEEECCCCCCHHHHHCCCEECCCCCCCCCCCCEEEE
IGGGNSGVEAAIDLAGIVEHVTLVEFDTKLRADQVLQDKLNSLPNATIITNALSTEVIGD
EECCCCCHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHCCC
GAQVTGLKYKDRATDTEHTIELAGIFVQIGLLPNTDFLKGSVELSPRGEIIINERNETNV
CCEEECEEECCCCCCCHHHHEEEEEEEEEECCCCCCCCCCEEEECCCCCEEEECCCCCCC
KGVFAAGDCTTVPYKQIIIATGEGAKASLSAFDYIIRHG
EEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLDQNIKTQLKAYLERLESPIELVAALDDSDKAAKIKELVTEIAELSNQVTARFDGQNTR
CCCCHHHHHHHHHHHHHCCHHHHHEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCC
RPSFGIAKAGEQPRVFFAGLPMGHEFTSLILALLQTSGYAPKVSDEILNQIKGLNLKADF
CCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEECC
DVFVSLSCHNCPDVVQALNLIAIYNPNSTATMIDGAFFQDEVEERKIMAVPMVFQNGNHI
EEEEEEECCCCHHHHHHCCEEEEECCCCCEEEECCHHHHHHHHCCEEEEEEEEEECCCEE
GQGRMTLEEIVAKLDSNAAEKDAAAINAKDVFDVLVVGAGPAGGTAAIYAARKGIRTGMI
CCCCHHHHHHHHHHCCCCCHHHHHHCCHHHHEEEEEEECCCCCCHHHHHHHHHCCHHCHH
AERFGGQVMDTMDIENFTSVQKTVGPKFAADMEAHVREYDVDIMNLQRVSKIVGADQTAS
HHHHCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCC
GLVEVELENGAKVESKTVILSTGARWRKMGVPGEQEYATRGVAYCPHCDGPLFKGKKVAV
CEEEEEECCCCEEECCEEEEECCCCEEECCCCCCHHHHHCCCEECCCCCCCCCCCCEEEE
IGGGNSGVEAAIDLAGIVEHVTLVEFDTKLRADQVLQDKLNSLPNATIITNALSTEVIGD
EECCCCCHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHCCC
GAQVTGLKYKDRATDTEHTIELAGIFVQIGLLPNTDFLKGSVELSPRGEIIINERNETNV
CCEEECEEECCCCCCCHHHHEEEEEEEEEECCCCCCCCCCEEEECCCCCEEEECCCCCCC
KGVFAAGDCTTVPYKQIIIATGEGAKASLSAFDYIIRHG
EEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]