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The map label for this gene is fadH [C]

Identifier: 50084337

GI number: 50084337

Start: 1134569

End: 1135813

Strand: Direct

Name: fadH [C]

Synonym: ACIAD1143

Alternate gene names: 50084337

Gene position: 1134569-1135813 (Clockwise)

Preceding gene: 50084336

Following gene: 50084345

Centisome position: 31.53

GC content: 43.37

Gene sequence:

>1245_bases
ATGAGTCAGATCGCTGAGCCAATCACAATACGAAATGTCACATTCAAAAATCGCATTATTAAAGGGGCAATGAGTGAAGC
GCTTGCCAATCGTGCAGGTCAACCTAATGATTTGCATTTTGGCTTGTATCAGGCTTGGGCGCAAGGAGGGCTGGGTTGTG
CCATCACAGGCAACGTGATGGTTGATTTGCGTGCCAAAAATGAACCTGGTGTAGTGGCGATTGAAAGTGAGCGTGATCTG
GAACGCTTAAAAAAATGGGCGGATATTGGGAAAAAATATGGCATGGTGCAGTTGATTCAATTGTCACATCCAGGTCGTCA
ATGTCCGAAAGGCCTAAATCAGGAAACAGTTGCTCCCTCTGCTGTGCCATTTAGCCCCATGCTAGCGACCATGTTTGCGA
CTCCGCGCGAATTACATCATGAAGAAATATTAGATATTATTCAGCGCTTTGCCGATGCTGCGCGTGTATGTGAAAAGGCT
GGTTTTGAAGGTGTACAGCTTCATGGTGCACATGGCTATTTAATCAGCCAGTTTTTATCACCACTGACCAATAAGCGCCA
AGATCAATGGGGTGGTTCAATTGAAAATAGAATGCGTTTTCTGCTCGAAATTTATCATGCTGTACGCGCAGCGACTTCAG
AGAATTTTATTATTTCGGTAAAACTGAATTCTGCCGATTTTCAGCGTGGTGGCATTACCGAAGAAGATGTCATTGCGGTA
TTCAAAGCAATTGATCAGGCAGGAATTGACTTGATTGAAGTCTCTGGAGGCACTTATGAAGCTCCTGCAATGGCAGGTGT
CAAAGCAGAAAAGCGTAAAGCGAGTACGATTGCACGGGAGGCTTACTTTTTAGAATTTGCCGAGAAAATTCGAGATTCTG
TGCAATGTAAACTGATGGTGACCGGAGGTTTTCGTAGTGTAGAGGGGATGAATGCAGCATTACAAAGCGGTGCATGTGAT
TTTATTGGAATTGCACGTCCTTTTGCGGTAGAAACAGATTTAACCCATCGGCTGATTGCGGGGCAAGACGTTAAATATGC
AGTGAAACCCATTAAAACTGGAGTGCCGTTTGTAGACAAAATGGCAGTTATGGAAATTATTTGGTATGCGGCGCAGTTTA
AAGCGATTGGTAAGGGGAAAAAGCCAAATCCAAAACTATCACCATTATTGGTATTTTTAAACTATGCCAAAGGTAATGTG
AAGGCGTTGATACAAGGTCGAATTAATTCTAGAAAATCGGCATAA

Upstream 100 bases:

>100_bases
TTGACAACTTGTATTGTCAATTTGTCGGTTTGCGCTTGACAGTCATTTCTATTGATTTAGATTGAAATAAATAACCTAAT
TATTTCTGTCGGATATCTTT

Downstream 100 bases:

>100_bases
GCCATAAAGTACAGTTTTGTATTGGGTAGGATTTAAAACTACAAACAAATAAGCCTCACAATTGTGAGGCTTACTGATGA
TCATAGTGATCGAATTTGGC

Product: FMN oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MSQIAEPITIRNVTFKNRIIKGAMSEALANRAGQPNDLHFGLYQAWAQGGLGCAITGNVMVDLRAKNEPGVVAIESERDL
ERLKKWADIGKKYGMVQLIQLSHPGRQCPKGLNQETVAPSAVPFSPMLATMFATPRELHHEEILDIIQRFADAARVCEKA
GFEGVQLHGAHGYLISQFLSPLTNKRQDQWGGSIENRMRFLLEIYHAVRAATSENFIISVKLNSADFQRGGITEEDVIAV
FKAIDQAGIDLIEVSGGTYEAPAMAGVKAEKRKASTIAREAYFLEFAEKIRDSVQCKLMVTGGFRSVEGMNAALQSGACD
FIGIARPFAVETDLTHRLIAGQDVKYAVKPIKTGVPFVDKMAVMEIIWYAAQFKAIGKGKKPNPKLSPLLVFLNYAKGNV
KALIQGRINSRKSA

Sequences:

>Translated_414_residues
MSQIAEPITIRNVTFKNRIIKGAMSEALANRAGQPNDLHFGLYQAWAQGGLGCAITGNVMVDLRAKNEPGVVAIESERDL
ERLKKWADIGKKYGMVQLIQLSHPGRQCPKGLNQETVAPSAVPFSPMLATMFATPRELHHEEILDIIQRFADAARVCEKA
GFEGVQLHGAHGYLISQFLSPLTNKRQDQWGGSIENRMRFLLEIYHAVRAATSENFIISVKLNSADFQRGGITEEDVIAV
FKAIDQAGIDLIEVSGGTYEAPAMAGVKAEKRKASTIAREAYFLEFAEKIRDSVQCKLMVTGGFRSVEGMNAALQSGACD
FIGIARPFAVETDLTHRLIAGQDVKYAVKPIKTGVPFVDKMAVMEIIWYAAQFKAIGKGKKPNPKLSPLLVFLNYAKGNV
KALIQGRINSRKSA
>Mature_413_residues
SQIAEPITIRNVTFKNRIIKGAMSEALANRAGQPNDLHFGLYQAWAQGGLGCAITGNVMVDLRAKNEPGVVAIESERDLE
RLKKWADIGKKYGMVQLIQLSHPGRQCPKGLNQETVAPSAVPFSPMLATMFATPRELHHEEILDIIQRFADAARVCEKAG
FEGVQLHGAHGYLISQFLSPLTNKRQDQWGGSIENRMRFLLEIYHAVRAATSENFIISVKLNSADFQRGGITEEDVIAVF
KAIDQAGIDLIEVSGGTYEAPAMAGVKAEKRKASTIAREAYFLEFAEKIRDSVQCKLMVTGGFRSVEGMNAALQSGACDF
IGIARPFAVETDLTHRLIAGQDVKYAVKPIKTGVPFVDKMAVMEIIWYAAQFKAIGKGKKPNPKLSPLLVFLNYAKGNVK
ALIQGRINSRKSA

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=340, Percent_Identity=29.4117647058824, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1787939, Length=354, Percent_Identity=28.5310734463277, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17559802, Length=355, Percent_Identity=40.5633802816901, Blast_Score=244, Evalue=8e-65,
Organism=Caenorhabditis elegans, GI17565138, Length=340, Percent_Identity=40.5882352941176, Blast_Score=240, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI17564188, Length=343, Percent_Identity=37.9008746355685, Blast_Score=228, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI17559804, Length=345, Percent_Identity=36.8115942028986, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI72001454, Length=350, Percent_Identity=38.2857142857143, Blast_Score=225, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI17540738, Length=353, Percent_Identity=37.6770538243626, Blast_Score=217, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17566914, Length=365, Percent_Identity=34.7945205479452, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17566916, Length=358, Percent_Identity=31.2849162011173, Blast_Score=155, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6325086, Length=225, Percent_Identity=29.7777777777778, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321973, Length=218, Percent_Identity=28.8990825688073, Blast_Score=77, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 45419; Mature: 45288

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQIAEPITIRNVTFKNRIIKGAMSEALANRAGQPNDLHFGLYQAWAQGGLGCAITGNVM
CCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEEEECCEE
VDLRAKNEPGVVAIESERDLERLKKWADIGKKYGMVQLIQLSHPGRQCPKGLNQETVAPS
EEEEECCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHCCCCCCCCCCCCC
AVPFSPMLATMFATPRELHHEEILDIIQRFADAARVCEKAGFEGVQLHGAHGYLISQFLS
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHH
PLTNKRQDQWGGSIENRMRFLLEIYHAVRAATSENFIISVKLNSADFQRGGITEEDVIAV
HHHCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHH
FKAIDQAGIDLIEVSGGTYEAPAMAGVKAEKRKASTIAREAYFLEFAEKIRDSVQCKLMV
HHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
TGGFRSVEGMNAALQSGACDFIGIARPFAVETDLTHRLIAGQDVKYAVKPIKTGVPFVDK
ECCCCCHHHHHHHHHCCCCCEEECCCCCEECCCHHHHHHCCCCCHHHHHHHHCCCCHHHH
MAVMEIIWYAAQFKAIGKGKKPNPKLSPLLVFLNYAKGNVKALIQGRINSRKSA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
SQIAEPITIRNVTFKNRIIKGAMSEALANRAGQPNDLHFGLYQAWAQGGLGCAITGNVM
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEEEECCEE
VDLRAKNEPGVVAIESERDLERLKKWADIGKKYGMVQLIQLSHPGRQCPKGLNQETVAPS
EEEEECCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHCCCCCCCCCCCCC
AVPFSPMLATMFATPRELHHEEILDIIQRFADAARVCEKAGFEGVQLHGAHGYLISQFLS
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHH
PLTNKRQDQWGGSIENRMRFLLEIYHAVRAATSENFIISVKLNSADFQRGGITEEDVIAV
HHHCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHH
FKAIDQAGIDLIEVSGGTYEAPAMAGVKAEKRKASTIAREAYFLEFAEKIRDSVQCKLMV
HHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
TGGFRSVEGMNAALQSGACDFIGIARPFAVETDLTHRLIAGQDVKYAVKPIKTGVPFVDK
ECCCCCHHHHHHHHHCCCCCEEECCCCCEECCCHHHHHHCCCCCHHHHHHHHCCCCHHHH
MAVMEIIWYAAQFKAIGKGKKPNPKLSPLLVFLNYAKGNVKALIQGRINSRKSA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]