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The map label for this gene is ubiF [H]

Identifier: 50084322

GI number: 50084322

Start: 1116049

End: 1117281

Strand: Direct

Name: ubiF [H]

Synonym: ACIAD1128

Alternate gene names: 50084322

Gene position: 1116049-1117281 (Clockwise)

Preceding gene: 50084321

Following gene: 50084323

Centisome position: 31.01

GC content: 45.66

Gene sequence:

>1233_bases
ATGACACAAATCGCAGATGTGGTAATTGTAGGTGGTGGATTGGTTGGTGGCCTAACGGCATTGCTGCTTGCCCAAGGTGG
TGTTCAAGCGACTGTACTGGATGCTGCGCCCATACTTGATACTGCAAAAGTTGAAAGTGTGATGAACCCGCGTGTGCTTG
CACTGAGTCAGGCAACAATTCATCTGCTTAAAACCGTTGAGGTATGGGAGCTTATTTCACGGCAAATGCCTTATACAGGG
ATGCAGGTGTGGAATAAAAATGGCTATGGTGATATTCAATTTGGACATCACAGCGCCTCAACGCCTATGATTGATCAGGC
ACTGGGTTCTATGGTCGAACCTAGTTTATTGAATCTTGCGATTCAGCAAAAAATGAGAGCCGTTATTCAGGATTATCGAA
CCGAGGTTCAGGTTGTTCAGATTGAACAACTGCCAAATCTTTGGCAGATCCATCTGGCTGATGGTCATTGTATCCAGACA
CGATTATTAATTGGTGCCGATGGCGCAAATTCGTTCGTACGCGAAAAGGCCATGATTGATCTGGATGTACTTGACTATAA
ACAAGCAGCAATAAGCTGTGCGATAAAAACAGCGCTCTCGCATGAGCATGTCGCTCGTCAAATTTTTCTGCCAACAGGGC
CTTTGGCCTTTTTGCCTATGGCAAGTCTCGTTCCAGCCGAGCAGGGTTTGTGGCAATCGATTGTTTGGACCTTACCAGAT
GATTATGCAGATGAGTATTCAGCGCTGTCGGATCATGAATTTAAAGCGTTGCTAACTCGTGAGAGTCAGCACATGCTAGG
TGAGGTTGTTGAAGTCCGATCAAGAGCTCAATTTCCATTAAAAGCTCGTGCAGCAAAACAATATGTGAAAGAGGGTCTGG
CATTGATTGGTGATGCCGCGCATGTGATCCATCCTCTTGCTGGACAAGGCGTAAACATTGGCTGTCTGGATGCTGCGGTT
TTGTGTGATGCATTACTGCATGATCGTGCGCGTGGTGTCTGGGCTCATGAGCAAACTTTGAGACGCTATGAACATGAGCG
TAAAGGTCAAAATGATGCCATGATGCATGGCATGTCGATCATTGGCTGGCTAGAAAGTCAGGAGTTTTTCCCGCTCATTG
CACTACGCAACACAGGGTTACGATGGGTCGAAAACAGTGAAATTCTTAAAAATTCATTTTTGCAACAAGCGACGGGATTA
AGCATTTTAAATAAGACTCGGTATGTGTCTTGA

Upstream 100 bases:

>100_bases
ATCCTATTCTTAAGTTATTGCGTAATACTGGTTTGATTGCTTTTGATGTGATTCCTGGAATCAAGCCGTTGGTTGCCAAT
TACGCTATGGGATTAAAAGC

Downstream 100 bases:

>100_bases
TCCATCTTCGCTATTTGTGAATCAAACATTGATCTAATCTTACAAATTAGATTAAAAAAATACGAAATTGTTGTAAAAGC
CTCTTTGCGAAAGCGCAAGA

Product: FAD-dependent monooxygenase

Products: 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol [C]

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MTQIADVVIVGGGLVGGLTALLLAQGGVQATVLDAAPILDTAKVESVMNPRVLALSQATIHLLKTVEVWELISRQMPYTG
MQVWNKNGYGDIQFGHHSASTPMIDQALGSMVEPSLLNLAIQQKMRAVIQDYRTEVQVVQIEQLPNLWQIHLADGHCIQT
RLLIGADGANSFVREKAMIDLDVLDYKQAAISCAIKTALSHEHVARQIFLPTGPLAFLPMASLVPAEQGLWQSIVWTLPD
DYADEYSALSDHEFKALLTRESQHMLGEVVEVRSRAQFPLKARAAKQYVKEGLALIGDAAHVIHPLAGQGVNIGCLDAAV
LCDALLHDRARGVWAHEQTLRRYEHERKGQNDAMMHGMSIIGWLESQEFFPLIALRNTGLRWVENSEILKNSFLQQATGL
SILNKTRYVS

Sequences:

>Translated_410_residues
MTQIADVVIVGGGLVGGLTALLLAQGGVQATVLDAAPILDTAKVESVMNPRVLALSQATIHLLKTVEVWELISRQMPYTG
MQVWNKNGYGDIQFGHHSASTPMIDQALGSMVEPSLLNLAIQQKMRAVIQDYRTEVQVVQIEQLPNLWQIHLADGHCIQT
RLLIGADGANSFVREKAMIDLDVLDYKQAAISCAIKTALSHEHVARQIFLPTGPLAFLPMASLVPAEQGLWQSIVWTLPD
DYADEYSALSDHEFKALLTRESQHMLGEVVEVRSRAQFPLKARAAKQYVKEGLALIGDAAHVIHPLAGQGVNIGCLDAAV
LCDALLHDRARGVWAHEQTLRRYEHERKGQNDAMMHGMSIIGWLESQEFFPLIALRNTGLRWVENSEILKNSFLQQATGL
SILNKTRYVS
>Mature_409_residues
TQIADVVIVGGGLVGGLTALLLAQGGVQATVLDAAPILDTAKVESVMNPRVLALSQATIHLLKTVEVWELISRQMPYTGM
QVWNKNGYGDIQFGHHSASTPMIDQALGSMVEPSLLNLAIQQKMRAVIQDYRTEVQVVQIEQLPNLWQIHLADGHCIQTR
LLIGADGANSFVREKAMIDLDVLDYKQAAISCAIKTALSHEHVARQIFLPTGPLAFLPMASLVPAEQGLWQSIVWTLPDD
YADEYSALSDHEFKALLTRESQHMLGEVVEVRSRAQFPLKARAAKQYVKEGLALIGDAAHVIHPLAGQGVNIGCLDAAVL
CDALLHDRARGVWAHEQTLRRYEHERKGQNDAMMHGMSIIGWLESQEFFPLIALRNTGLRWVENSEILKNSFLQQATGLS
ILNKTRYVS

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=417, Percent_Identity=29.7362110311751, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI32967307, Length=288, Percent_Identity=32.9861111111111, Blast_Score=119, Evalue=6e-27,
Organism=Escherichia coli, GI1786883, Length=406, Percent_Identity=34.4827586206897, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1789273, Length=406, Percent_Identity=36.2068965517241, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1789274, Length=405, Percent_Identity=26.4197530864197, Blast_Score=114, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI25146631, Length=439, Percent_Identity=31.2072892938497, Blast_Score=132, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6321694, Length=464, Percent_Identity=27.1551724137931, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI19920760, Length=430, Percent_Identity=29.7674418604651, Blast_Score=158, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 45229; Mature: 45098

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQIADVVIVGGGLVGGLTALLLAQGGVQATVLDAAPILDTAKVESVMNPRVLALSQATI
CCCEEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCEEEHHHHHH
HLLKTVEVWELISRQMPYTGMQVWNKNGYGDIQFGHHSASTPMIDQALGSMVEPSLLNLA
HHHHHHHHHHHHHHCCCCCCHHEECCCCCCCEEECCCCCCCCHHHHHHHHHCCHHHHHHH
IQQKMRAVIQDYRTEVQVVQIEQLPNLWQIHLADGHCIQTRLLIGADGANSFVREKAMID
HHHHHHHHHHHHHHHEEEEECCCCCCEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHEE
LDVLDYKQAAISCAIKTALSHEHVARQIFLPTGPLAFLPMASLVPAEQGLWQSIVWTLPD
EHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCHHCHHHHHHCCCCC
DYADEYSALSDHEFKALLTRESQHMLGEVVEVRSRAQFPLKARAAKQYVKEGLALIGDAA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHH
HVIHPLAGQGVNIGCLDAAVLCDALLHDRARGVWAHEQTLRRYEHERKGQNDAMMHGMSI
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
IGWLESQEFFPLIALRNTGLRWVENSEILKNSFLQQATGLSILNKTRYVS
HHHHCCCCCCEEEEEECCCCEEECCHHHHHHHHHHHHHCHHHHHHHCCCC
>Mature Secondary Structure 
TQIADVVIVGGGLVGGLTALLLAQGGVQATVLDAAPILDTAKVESVMNPRVLALSQATI
CCEEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCEEEHHHHHH
HLLKTVEVWELISRQMPYTGMQVWNKNGYGDIQFGHHSASTPMIDQALGSMVEPSLLNLA
HHHHHHHHHHHHHHCCCCCCHHEECCCCCCCEEECCCCCCCCHHHHHHHHHCCHHHHHHH
IQQKMRAVIQDYRTEVQVVQIEQLPNLWQIHLADGHCIQTRLLIGADGANSFVREKAMID
HHHHHHHHHHHHHHHEEEEECCCCCCEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHEE
LDVLDYKQAAISCAIKTALSHEHVARQIFLPTGPLAFLPMASLVPAEQGLWQSIVWTLPD
EHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCHHCHHHHHHCCCCC
DYADEYSALSDHEFKALLTRESQHMLGEVVEVRSRAQFPLKARAAKQYVKEGLALIGDAA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHH
HVIHPLAGQGVNIGCLDAAVLCDALLHDRARGVWAHEQTLRRYEHERKGQNDAMMHGMSI
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
IGWLESQEFFPLIALRNTGLRWVENSEILKNSFLQQATGLSILNKTRYVS
HHHHCCCCCCEEEEEECCCCEEECCHHHHHHHHHHHHHCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: @OCTA02.txt*2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol! [C]

Specific reaction: @OCTA02.txt*2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol! = 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol [C]

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]