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The map label for this gene is gdhA [H]

Identifier: 50084304

GI number: 50084304

Start: 1097660

End: 1099003

Strand: Direct

Name: gdhA [H]

Synonym: ACIAD1110

Alternate gene names: 50084304

Gene position: 1097660-1099003 (Clockwise)

Preceding gene: 50084301

Following gene: 50084316

Centisome position: 30.5

GC content: 39.73

Gene sequence:

>1344_bases
TTGAAATACAGCAAAATGAGTGATTTTTTGGATTACGTTAAAACACGTGATCAGTACCAACCTGAATTTTTGCAGGCTGT
TGAAGAGGTGATGATAAGTTTATGGCCGTTTATTGAAAAGCACCCAGAATATGCAGATTACGGATTATTAGAAAGACTGA
TTGAACCTGAACGAGTCATTCAATTTCGTGTGGCATGGATGGATGACCAAGGTCATACCCAAGTTAATCGTGCATTTCGT
GTGCAATACAATTCTGCGATTGGTCCATATAAAGGCGGAATGCGTTTTCATCCTTCAGTAAATTTATCTATTTTAAAATT
TTTGGGTTTTGAACAGACATTTAAGAATGCATTAACTACTTTGCCAATGGGGGGAGGTAAGGGCGGTTCTGATTTTAATC
CGAAAAATAAATCAGAAGCAGAAATCATGCGTTTTTGTCAGGCATTAATGCTTGAACTTCATCGTCATTTAGGTGCAGAT
ACGGATATTCCAGCTGGAGATATTGGGGTCGGCGCTCGTGAAGTTGGTTATATGGCGGGAATGATGAAAAAGCTGAGCAA
TGATACGGCAGCTGTTTTTACAGGAAAAGGTGTCTCATTTGGTGGAAGTCTGATGCGTCCTGAAGCTACAGGATATGGAA
CTGTATATTTTGCCGAAGAAATGCTCAAAACACGTGGTCAAAATTTTCTAGGAAAAACAGTTTCAATCTCTGGCTCTGGT
AATGTGGCCCAATATGCAGCCGAAAAGGCGATGTTTTTGGGCGCAAAAGTGGTTACATTGTCCGACTCAAATGGTACTGT
ATATTTAGAAAATGGTTTCACCGAAGTGCTTCTAGCCGAATTAATGGAATTAAAAAATAAAAAGCGTGGACGTATTTCAG
AATTCGCCTCTAAACACGGATTTGAATATTTTGAAGGTCAAACACCTTGGCATATTCCAGTTGATATTGCTTTGCCATGC
GCAACCCAAAATGAGTTGAATGGAGATGATGCTGAAACTTTGATTAAAAACGGAGTGATTTGTGTGGCAGAAGGTGCGAA
TATGCCATCTACACTCGAAGCAGTAGAAAAATTTCTGCATGCAAAAATCTTGTATGCACCTGGTAAGGCATCAAATGCTG
GAGGGGTTGCAACCTCTGGACTTGAAATGTCTCAAAACTCTATCCGACTCAACTGGTCTCATGCCGAAGTAGATGAAAGA
TTACATGCGATTATGAAAGATATTCATGCCAATTGTGTCAAGTACGGTACCAAACAAGATGGTTCAATTAACTATGTTGA
TGGGGCAAATATTGCAGGTTTTGTCAAAGTTGCAGATGCCATGTTGGCACAAGGGATTTACTAA

Upstream 100 bases:

>100_bases
GAGAAAAATTTATAAAAAGTATTTAATTTAGTGCTTTAAAAGTTTTCATTTTTTTAAAAATTGCACAATATTGATTCACT
TTATGAGAGAGGATGAAGCG

Downstream 100 bases:

>100_bases
TCTTATTGTAATGAAATGAAAAAATCCGATGCTGTTGCACCGGATTTAAAAAATAATGCGGATTATGGTTTTTGAACAAT
TTCCTGTTCAACAATCATAT

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MKYSKMSDFLDYVKTRDQYQPEFLQAVEEVMISLWPFIEKHPEYADYGLLERLIEPERVIQFRVAWMDDQGHTQVNRAFR
VQYNSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFNPKNKSEAEIMRFCQALMLELHRHLGAD
TDIPAGDIGVGAREVGYMAGMMKKLSNDTAAVFTGKGVSFGGSLMRPEATGYGTVYFAEEMLKTRGQNFLGKTVSISGSG
NVAQYAAEKAMFLGAKVVTLSDSNGTVYLENGFTEVLLAELMELKNKKRGRISEFASKHGFEYFEGQTPWHIPVDIALPC
ATQNELNGDDAETLIKNGVICVAEGANMPSTLEAVEKFLHAKILYAPGKASNAGGVATSGLEMSQNSIRLNWSHAEVDER
LHAIMKDIHANCVKYGTKQDGSINYVDGANIAGFVKVADAMLAQGIY

Sequences:

>Translated_447_residues
MKYSKMSDFLDYVKTRDQYQPEFLQAVEEVMISLWPFIEKHPEYADYGLLERLIEPERVIQFRVAWMDDQGHTQVNRAFR
VQYNSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFNPKNKSEAEIMRFCQALMLELHRHLGAD
TDIPAGDIGVGAREVGYMAGMMKKLSNDTAAVFTGKGVSFGGSLMRPEATGYGTVYFAEEMLKTRGQNFLGKTVSISGSG
NVAQYAAEKAMFLGAKVVTLSDSNGTVYLENGFTEVLLAELMELKNKKRGRISEFASKHGFEYFEGQTPWHIPVDIALPC
ATQNELNGDDAETLIKNGVICVAEGANMPSTLEAVEKFLHAKILYAPGKASNAGGVATSGLEMSQNSIRLNWSHAEVDER
LHAIMKDIHANCVKYGTKQDGSINYVDGANIAGFVKVADAMLAQGIY
>Mature_447_residues
MKYSKMSDFLDYVKTRDQYQPEFLQAVEEVMISLWPFIEKHPEYADYGLLERLIEPERVIQFRVAWMDDQGHTQVNRAFR
VQYNSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFNPKNKSEAEIMRFCQALMLELHRHLGAD
TDIPAGDIGVGAREVGYMAGMMKKLSNDTAAVFTGKGVSFGGSLMRPEATGYGTVYFAEEMLKTRGQNFLGKTVSISGSG
NVAQYAAEKAMFLGAKVVTLSDSNGTVYLENGFTEVLLAELMELKNKKRGRISEFASKHGFEYFEGQTPWHIPVDIALPC
ATQNELNGDDAETLIKNGVICVAEGANMPSTLEAVEKFLHAKILYAPGKASNAGGVATSGLEMSQNSIRLNWSHAEVDER
LHAIMKDIHANCVKYGTKQDGSINYVDGANIAGFVKVADAMLAQGIY

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=401, Percent_Identity=29.426433915212, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI4885281, Length=401, Percent_Identity=28.927680798005, Blast_Score=132, Evalue=5e-31,
Organism=Escherichia coli, GI1788059, Length=441, Percent_Identity=70.2947845804989, Blast_Score=649, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544676, Length=355, Percent_Identity=29.2957746478873, Blast_Score=121, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6324951, Length=451, Percent_Identity=51.6629711751663, Blast_Score=415, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6319256, Length=453, Percent_Identity=50.551876379691, Blast_Score=401, Evalue=1e-113,
Organism=Drosophila melanogaster, GI45553475, Length=351, Percent_Identity=28.7749287749288, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI45549226, Length=351, Percent_Identity=28.7749287749288, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24649283, Length=330, Percent_Identity=30.6060606060606, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24649460, Length=290, Percent_Identity=29.3103448275862, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 49147; Mature: 49147

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYSKMSDFLDYVKTRDQYQPEFLQAVEEVMISLWPFIEKHPEYADYGLLERLIEPERVI
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHEE
QFRVAWMDDQGHTQVNRAFRVQYNSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALTT
EEEEEEECCCCCHHHCCEEEEEECCCCCCCCCCEEECCCCCHHHHHHHCHHHHHHHHHHH
LPMGGGKGGSDFNPKNKSEAEIMRFCQALMLELHRHLGADTDIPAGDIGVGAREVGYMAG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
MMKKLSNDTAAVFTGKGVSFGGSLMRPEATGYGTVYFAEEMLKTRGQNFLGKTVSISGSG
HHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCC
NVAQYAAEKAMFLGAKVVTLSDSNGTVYLENGFTEVLLAELMELKNKKRGRISEFASKHG
CHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHCC
FEYFEGQTPWHIPVDIALPCATQNELNGDDAETLIKNGVICVAEGANMPSTLEAVEKFLH
CHHHCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHCCCEEEECCCCCCHHHHHHHHHHH
AKILYAPGKASNAGGVATSGLEMSQNSIRLNWSHAEVDERLHAIMKDIHANCVKYGTKQD
HEEEECCCCCCCCCCCEECCCEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCC
GSINYVDGANIAGFVKVADAMLAQGIY
CCEEEECCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKYSKMSDFLDYVKTRDQYQPEFLQAVEEVMISLWPFIEKHPEYADYGLLERLIEPERVI
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHEE
QFRVAWMDDQGHTQVNRAFRVQYNSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALTT
EEEEEEECCCCCHHHCCEEEEEECCCCCCCCCCEEECCCCCHHHHHHHCHHHHHHHHHHH
LPMGGGKGGSDFNPKNKSEAEIMRFCQALMLELHRHLGADTDIPAGDIGVGAREVGYMAG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
MMKKLSNDTAAVFTGKGVSFGGSLMRPEATGYGTVYFAEEMLKTRGQNFLGKTVSISGSG
HHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCC
NVAQYAAEKAMFLGAKVVTLSDSNGTVYLENGFTEVLLAELMELKNKKRGRISEFASKHG
CHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHCC
FEYFEGQTPWHIPVDIALPCATQNELNGDDAETLIKNGVICVAEGANMPSTLEAVEKFLH
CHHHCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHCCCEEEECCCCCCHHHHHHHHHHH
AKILYAPGKASNAGGVATSGLEMSQNSIRLNWSHAEVDERLHAIMKDIHANCVKYGTKQD
HEEEECCCCCCCCCCCEECCCEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCC
GSINYVDGANIAGFVKVADAMLAQGIY
CCEEEECCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]