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The map label for this gene is nlpE [H]

Identifier: 50084283

GI number: 50084283

Start: 1081561

End: 1082037

Strand: Reverse

Name: nlpE [H]

Synonym: ACIAD1085

Alternate gene names: 50084283

Gene position: 1082037-1081561 (Counterclockwise)

Preceding gene: 50084284

Following gene: 50084280

Centisome position: 30.07

GC content: 38.57

Gene sequence:

>477_bases
ATGAAAAAATCATTATTAGTCGTTGCTCTGACAAGCGTTGCTGTGGTCGCCTGTTCTAAATCTGAAAATAAAACCGAACA
AACACCTACCGCAGAACATACCATAGTCGCTTCCTCAGCTACACATTCAGAAACTCAACATGATATGCACACTGCAGAGT
ATTCCTTAGATTGGGCAGGAAAATATACTGGTAAATTACCTTGTGCAGACTGCGAAGGTATTCAAACTGCACTTGAGCTC
AAAAATGATAAAACCTATGAACTCAAAGAAACCTATATGGGTGGCAAAAGTGATGGAAAACCTTTTGAAACACAAGGTAC
GTTCACTTTTGATCAGAAAAATCCTTCAATGATTGTGTTAGATCAAAAGTCCGAAAACCGCAAGTTTTTCGTTGCTGAAA
ATACGCTGACCGCACTTGATATGGAAGGCAATAAAATCGAAGGTGCCATGGCCGATCTTTATATCTTGAAAAAATAA

Upstream 100 bases:

>100_bases
TTGTTAAAAAAATGGGCTTTCTTACATTTTTCTTACTTTAGTTTGAAGTTATATTTATTAGTCTTGTCAGCTTCTCAAAT
AAAAAAGATTGGATTACATA

Downstream 100 bases:

>100_bases
AATCATGTTTTTAACTCTAAAATAAAAAACCCTGCTTATGCAGGGTTTTTTAAACCATACGCCTTAGTGAGCGAACTGGT
TCATAGTGTTTTTGCTTGCA

Product: lipoprotein

Products: NA

Alternate protein names: Copper homeostasis protein CutF [H]

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MKKSLLVVALTSVAVVACSKSENKTEQTPTAEHTIVASSATHSETQHDMHTAEYSLDWAGKYTGKLPCADCEGIQTALEL
KNDKTYELKETYMGGKSDGKPFETQGTFTFDQKNPSMIVLDQKSENRKFFVAENTLTALDMEGNKIEGAMADLYILKK

Sequences:

>Translated_158_residues
MKKSLLVVALTSVAVVACSKSENKTEQTPTAEHTIVASSATHSETQHDMHTAEYSLDWAGKYTGKLPCADCEGIQTALEL
KNDKTYELKETYMGGKSDGKPFETQGTFTFDQKNPSMIVLDQKSENRKFFVAENTLTALDMEGNKIEGAMADLYILKK
>Mature_158_residues
MKKSLLVVALTSVAVVACSKSENKTEQTPTAEHTIVASSATHSETQHDMHTAEYSLDWAGKYTGKLPCADCEGIQTALEL
KNDKTYELKETYMGGKSDGKPFETQGTFTFDQKNPSMIVLDQKSENRKFFVAENTLTALDMEGNKIEGAMADLYILKK

Specific function: Involved in copper homeostasis. Could be involved in both copper efflux and the delivery of copper to copper-dependent enzymes. When overproduced induces degP through the activation of the two-component system CpxA/CpxR [H]

COG id: COG3015

COG function: function code MP; Uncharacterized lipoprotein NlpE involved in copper resistance

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786390, Length=86, Percent_Identity=37.2093023255814, Blast_Score=66, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007298 [H]

Pfam domain/function: PF04170 NlpE [H]

EC number: NA

Molecular weight: Translated: 17437; Mature: 17437

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSLLVVALTSVAVVACSKSENKTEQTPTAEHTIVASSATHSETQHDMHTAEYSLDWAG
CCCCEEEEEECCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCHHCCHHHCEEEEEECC
KYTGKLPCADCEGIQTALELKNDKTYELKETYMGGKSDGKPFETQGTFTFDQKNPSMIVL
CCCCCCCCCCCCHHHHHEECCCCCCEEEHHHHCCCCCCCCCCCCCCCEEECCCCCCEEEE
DQKSENRKFFVAENTLTALDMEGNKIEGAMADLYILKK
ECCCCCCEEEEECCCEEEEECCCCEECCCEEEEEEECC
>Mature Secondary Structure
MKKSLLVVALTSVAVVACSKSENKTEQTPTAEHTIVASSATHSETQHDMHTAEYSLDWAG
CCCCEEEEEECCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCHHCCHHHCEEEEEECC
KYTGKLPCADCEGIQTALELKNDKTYELKETYMGGKSDGKPFETQGTFTFDQKNPSMIVL
CCCCCCCCCCCCHHHHHEECCCCCCEEEHHHHCCCCCCCCCCCCCCCEEECCCCCCEEEE
DQKSENRKFFVAENTLTALDMEGNKIEGAMADLYILKK
ECCCCCCEEEEECCCEEEEECCCCEECCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7635808; 7635807; 9278503 [H]