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The map label for this gene is hsaD [H]

Identifier: 50084198

GI number: 50084198

Start: 980194

End: 981033

Strand: Direct

Name: hsaD [H]

Synonym: ACIAD0994

Alternate gene names: 50084198

Gene position: 980194-981033 (Clockwise)

Preceding gene: 50084197

Following gene: 50084199

Centisome position: 27.24

GC content: 42.62

Gene sequence:

>840_bases
ATGATGATGACACTTGTTGAACGCTTACAGAAATTTTCGGTGAAGTCTGTCGATATTGATGGGCAGACCCAAACCTACCG
TGAAGCAGGTACAGGAGATCAGTATCTGGTGTTGTTACATGGTATTAGCTCTGGTTCTGGTTCTTGGGTCAATCAACTCG
AAGCATTAAGTCATCATTTTCATGTAATTGCATGGGATGCACCTGGTTATGGCGCATCTGATGCTTTAACAACTTTAACG
CCAAATGCAGAGCATTATGCACAGCGTCTGGCTGGACTATTTCAGCAATTAGGCATTCAAAAAGCCATTGTGGTTGGGCA
TTCATTAGGGGCACTGCAAGCCAGTGCCTTTGCCCGAAATTATCCAGAGCATGTTGAGATATTAATGATTGCAAATGTTG
CACAGGGTTATCAGCGTTTTCCTGCTGTAAAGCAAGCGGAAATTTATCAAAAAAGACCAAAAATGCTGGCCGAACTTGGG
CATCAGGGATTAGCACAGTCCCGAGGCCCACATTTAACTTATTTAAAAAAACCAGAAACCTTAGAGCTGATTGAACAGGT
CATGCAAAACCTCAATCTGGGAGGTTTTACTCAAGCCTCTTATTTACTGGCTCATGATGAGATTCGAAATTATTTAACAG
ATTTGGCTGTGCCGTGCTTCGTCATTGCAGGCTTGTGCGACAGCATTACACCACCACAAGGGATTGCAGATTTGGTTCGG
GAAATTGATTGCCAGAACTATATCGAGATTAGTGAGGCAGGACATCTAAGTTATGTCGATCAAGCTGAATTATTTAACGA
CATTATCGTTTCTGCCAAGCAGCAACAAAACGAGAGATAA

Upstream 100 bases:

>100_bases
CCACCTGAGCATCCACTGTCTCAGATTAAACGTAACTAATTGATCATTAAGTAGAACGATTAAGACGGATTGATGTCTTA
ATGCTCAGCACGATAAGGAT

Downstream 100 bases:

>100_bases
AGTATGAGCTTCCAAGTAGAAGGAAAAGTAGCAGTGGTCACAGGTGGCTCTTCTGGCATCGGATTGGCTGCAGTTGAGAT
TTTAGTGGCTGAAGGTGCAA

Product: hydrolase

Products: NA

Alternate protein names: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase; HOPDA hydrolase; Meta-cleavage product hydrolase; MCP hydrolase [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MMMTLVERLQKFSVKSVDIDGQTQTYREAGTGDQYLVLLHGISSGSGSWVNQLEALSHHFHVIAWDAPGYGASDALTTLT
PNAEHYAQRLAGLFQQLGIQKAIVVGHSLGALQASAFARNYPEHVEILMIANVAQGYQRFPAVKQAEIYQKRPKMLAELG
HQGLAQSRGPHLTYLKKPETLELIEQVMQNLNLGGFTQASYLLAHDEIRNYLTDLAVPCFVIAGLCDSITPPQGIADLVR
EIDCQNYIEISEAGHLSYVDQAELFNDIIVSAKQQQNER

Sequences:

>Translated_279_residues
MMMTLVERLQKFSVKSVDIDGQTQTYREAGTGDQYLVLLHGISSGSGSWVNQLEALSHHFHVIAWDAPGYGASDALTTLT
PNAEHYAQRLAGLFQQLGIQKAIVVGHSLGALQASAFARNYPEHVEILMIANVAQGYQRFPAVKQAEIYQKRPKMLAELG
HQGLAQSRGPHLTYLKKPETLELIEQVMQNLNLGGFTQASYLLAHDEIRNYLTDLAVPCFVIAGLCDSITPPQGIADLVR
EIDCQNYIEISEAGHLSYVDQAELFNDIIVSAKQQQNER
>Mature_279_residues
MMMTLVERLQKFSVKSVDIDGQTQTYREAGTGDQYLVLLHGISSGSGSWVNQLEALSHHFHVIAWDAPGYGASDALTTLT
PNAEHYAQRLAGLFQQLGIQKAIVVGHSLGALQASAFARNYPEHVEILMIANVAQGYQRFPAVKQAEIYQKRPKMLAELG
HQGLAQSRGPHLTYLKKPETLELIEQVMQNLNLGGFTQASYLLAHDEIRNYLTDLAVPCFVIAGLCDSITPPQGIADLVR
EIDCQNYIEISEAGHLSYVDQAELFNDIIVSAKQQQNER

Specific function: Catalyzes the hydrolysis of a carbon-carbon bond in 4,5- 9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid (4,9-DSHA) to yield 9,17-dioxo-1,2,3,4,10,19- hexanorandrostan-5-oic acid (DOHNAA) and 2-hydroxy-hexa-2,4- dienoic acid (HHD). Is

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. HsaD family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=115, Percent_Identity=31.304347826087, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.7.1.8 [H]

Molecular weight: Translated: 30876; Mature: 30876

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMMTLVERLQKFSVKSVDIDGQTQTYREAGTGDQYLVLLHGISSGSGSWVNQLEALSHHF
CHHHHHHHHHHCCCCEECCCCCHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCE
HVIAWDAPGYGASDALTTLTPNAEHYAQRLAGLFQQLGIQKAIVVGHSLGALQASAFARN
EEEEECCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHCC
YPEHVEILMIANVAQGYQRFPAVKQAEIYQKRPKMLAELGHQGLAQSRGPHLTYLKKPET
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCH
LELIEQVMQNLNLGGFTQASYLLAHDEIRNYLTDLAVPCFVIAGLCDSITPPQGIADLVR
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
EIDCQNYIEISEAGHLSYVDQAELFNDIIVSAKQQQNER
HHCCCCCEEHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMMTLVERLQKFSVKSVDIDGQTQTYREAGTGDQYLVLLHGISSGSGSWVNQLEALSHHF
CHHHHHHHHHHCCCCEECCCCCHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCE
HVIAWDAPGYGASDALTTLTPNAEHYAQRLAGLFQQLGIQKAIVVGHSLGALQASAFARN
EEEEECCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHCC
YPEHVEILMIANVAQGYQRFPAVKQAEIYQKRPKMLAELGHQGLAQSRGPHLTYLKKPET
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCH
LELIEQVMQNLNLGGFTQASYLLAHDEIRNYLTDLAVPCFVIAGLCDSITPPQGIADLVR
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
EIDCQNYIEISEAGHLSYVDQAELFNDIIVSAKQQQNER
HHCCCCCEEHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]