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The map label for this gene is pcaR [H]

Identifier: 50084189

GI number: 50084189

Start: 972542

End: 973354

Strand: Reverse

Name: pcaR [H]

Synonym: ACIAD0985

Alternate gene names: 50084189

Gene position: 973354-972542 (Counterclockwise)

Preceding gene: 50084205

Following gene: 50084188

Centisome position: 27.05

GC content: 39.98

Gene sequence:

>813_bases
ATGAGTGTAGTCATTGAAGAAGATGTACAAGAGGCTGAAAGTACTAAAAACGATGATCGCTACTTGGTTCCAGGGCTGGT
TCGAGGCCTCGCGATTTTGCAAGCCTTTAATCAAGAATCTCAAGAAATGACCATTTCTGAAATTGCAGAAATTTTGGATG
TGAACCGTTCTAGTGCATTTCGTTTGATTTATACGCTGGAATCTTGCGGATATTTGCGTAAGGCAACTCAAAAAACCTAT
GCCCTAGATTCAAAAGTCATGGAGCTTGGTTTTAACAGTTTATCCAAACTGTCCTTACTTGATTTATCTACCCCCTTAAT
GAAAGAACTACGCGATCAAACCAAGATTGCAGTCCATCTTTCAATTTTGGAAGGTACACATATTGTCTTTGTAAACAATA
TTCAATCGAGTGGCCCTTTTACCAGTACGATTAGTTTAGGAACGCGCTGGCCTGCACATGCGACTGTGATTGGTCAGCTA
TTGCTATCCAATTTATCAGAAACTGAAATTCGTCAGCGTTATCGTAAATTTGATGAATGGGATACCTTCTCGGAACTCAC
GCCAAAAAACTTAAAAGAATTATTATTGCGTCTGAATTATGTCAAAACTCAGCCGTCCATGGTCAGTTGGGGACATTACA
ATAATGATATGGCTGCCTGTGCAGCACCCATTTATCGTCAATCCTCCAAAGATATGGTGGCAGTGTTGTCTGTAAGTTGC
CCTATCACCACTTACGATGAATATACGTTTAAACATAGTGTCGCGGCTCAGGTCATCGAGACAGCAGATAAAATCTCTAA
ATTTATCTATTGA

Upstream 100 bases:

>100_bases
ATAAACATTGTTTTATCACTTCGATATGATTAGAATAGTTCCAAAATAAAGCACAACGCACCAACATCACTCCCTTTTTA
CTTTTAGGAATTTTTATAGA

Downstream 100 bases:

>100_bases
TGTATATGGCAAAGGATTGCCATCACTTAAAATATTCAGCATGAAATAAAGTCTAATTAAAAATGCGCCGTATATCCCCT
TACGATGCCTCTCTTTCTAT

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MSVVIEEDVQEAESTKNDDRYLVPGLVRGLAILQAFNQESQEMTISEIAEILDVNRSSAFRLIYTLESCGYLRKATQKTY
ALDSKVMELGFNSLSKLSLLDLSTPLMKELRDQTKIAVHLSILEGTHIVFVNNIQSSGPFTSTISLGTRWPAHATVIGQL
LLSNLSETEIRQRYRKFDEWDTFSELTPKNLKELLLRLNYVKTQPSMVSWGHYNNDMAACAAPIYRQSSKDMVAVLSVSC
PITTYDEYTFKHSVAAQVIETADKISKFIY

Sequences:

>Translated_270_residues
MSVVIEEDVQEAESTKNDDRYLVPGLVRGLAILQAFNQESQEMTISEIAEILDVNRSSAFRLIYTLESCGYLRKATQKTY
ALDSKVMELGFNSLSKLSLLDLSTPLMKELRDQTKIAVHLSILEGTHIVFVNNIQSSGPFTSTISLGTRWPAHATVIGQL
LLSNLSETEIRQRYRKFDEWDTFSELTPKNLKELLLRLNYVKTQPSMVSWGHYNNDMAACAAPIYRQSSKDMVAVLSVSC
PITTYDEYTFKHSVAAQVIETADKISKFIY
>Mature_269_residues
SVVIEEDVQEAESTKNDDRYLVPGLVRGLAILQAFNQESQEMTISEIAEILDVNRSSAFRLIYTLESCGYLRKATQKTYA
LDSKVMELGFNSLSKLSLLDLSTPLMKELRDQTKIAVHLSILEGTHIVFVNNIQSSGPFTSTISLGTRWPAHATVIGQLL
LSNLSETEIRQRYRKFDEWDTFSELTPKNLKELLLRLNYVKTQPSMVSWGHYNNDMAACAAPIYRQSSKDMVAVLSVSCP
ITTYDEYTFKHSVAAQVIETADKISKFIY

Specific function: Positive regulator of all genes within the pca regulon, pcaBDC, pcaIJ and pcaF. Also required for the chemotactic response to aromatic compounds [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1788131, Length=238, Percent_Identity=26.890756302521, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1786468, Length=221, Percent_Identity=25.3393665158371, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788581, Length=250, Percent_Identity=23.6, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87082359, Length=252, Percent_Identity=22.6190476190476, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1786715, Length=242, Percent_Identity=25.2066115702479, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1789999, Length=140, Percent_Identity=22.8571428571429, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012794
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 30508; Mature: 30377

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVIEEDVQEAESTKNDDRYLVPGLVRGLAILQAFNQESQEMTISEIAEILDVNRSSAF
CCEEEHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHCCCCCCE
RLIYTLESCGYLRKATQKTYALDSKVMELGFNSLSKLSLLDLSTPLMKELRDQTKIAVHL
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHEEEEEE
SILEGTHIVFVNNIQSSGPFTSTISLGTRWPAHATVIGQLLLSNLSETEIRQRYRKFDEW
EEECCCEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
DTFSELTPKNLKELLLRLNYVKTQPSMVSWGHYNNDMAACAAPIYRQSSKDMVAVLSVSC
CCHHHCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEECC
PITTYDEYTFKHSVAAQVIETADKISKFIY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVVIEEDVQEAESTKNDDRYLVPGLVRGLAILQAFNQESQEMTISEIAEILDVNRSSAF
CEEEHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHCCCCCCE
RLIYTLESCGYLRKATQKTYALDSKVMELGFNSLSKLSLLDLSTPLMKELRDQTKIAVHL
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHEEEEEE
SILEGTHIVFVNNIQSSGPFTSTISLGTRWPAHATVIGQLLLSNLSETEIRQRYRKFDEW
EEECCCEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
DTFSELTPKNLKELLLRLNYVKTQPSMVSWGHYNNDMAACAAPIYRQSSKDMVAVLSVSC
CCHHHCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEECC
PITTYDEYTFKHSVAAQVIETADKISKFIY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8083169 [H]