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The map label for this gene is vanK [H]

Identifier: 50084186

GI number: 50084186

Start: 968383

End: 969729

Strand: Direct

Name: vanK [H]

Synonym: ACIAD0982

Alternate gene names: 50084186

Gene position: 968383-969729 (Clockwise)

Preceding gene: 50084182

Following gene: 50084187

Centisome position: 26.91

GC content: 42.17

Gene sequence:

>1347_bases
ATGCAAAGAGACATTTTAAGCGAAATTAATCAGAATAATATGAGCCGTTATCAATGGTTTGTGATCGTTGTTTGCGTGTG
TCTCAATATTATTGATGGCTTTGATGTGATGGTCATGGCATTTACAGCTCCATCTGTTTCAGCTGAGTGGTCACTTTCAG
GTGCACAAATCGGGCTTCTACTTAGTGCTGGTTTGTTTGGCATGGCTGCGGGTTCTATTTTTCTGGCACCTCTGGCAGAC
AAAATTGGTCGCCGTTTACTGATTTTAATTTGTTTAGCTATTGCAGGCATCAGCATGCTCGCCTGTGGTTTGGTGCATAG
CCACAGTATGTTGGCAGCATTACGCTTTGTGACAGGTATTGGGGTGGGGGGTATTTTAGCCAGTAGCAACGTTTTGGCCA
GTGAATATGCCAATTTACGCTGGCGTAGTCTTGCTGTTAGTCTCATGTCAACGGGGTATGGGATTGGCGCAACATTAGGC
GGCATATTGTCATTACTATTGATTGAGCATTTAGGCTGGCGTTCGATCTTTCTGGCGGGTGGTGTGACGACAGTTGTAAT
GTTATTGGTGAGCTTATGGTTATTACCAGAATCACTGGATTATTTATTGGCGAAGAAACCGAAGCAAGCACTAGAAAAAA
TCAATCTGACCGTGCAGCGTATCGGACTTGTTCGCTTAAATGCCTTACCACAGCATGAAAAAAATACACAACAGCACAGC
AGTGATATTGCCAAGCTTTTTCAAGGCAAGCAGGGATTTCAAACATTATGTTTGTGGTTTGCTTTCTTTCTGGTCATGTT
CGGTTTTTATTTTGTGATGAGCTGGACACCAAAAATTCTGATTGCAATGGGCATGTCGCCAAGTCAGGGCGTAACGACAG
GAATCTTAATTAGTATTGGTGGTGTTTTTGGTGCTGCAATTATTGGTCTTTTGGCATCACGTATTAAAATCTTTCATGCC
TTAAGTCTCTTTTTGGGATTAACCGCTGTGTGTGTATGGTTGTTCGTGGCCGTATCTTCGCAAGTGAGTATTGCACTCAT
GGTCGGTTTATTGCTTGGTACACTCATTAATGGCTGTGTGGCGGGTTTATATTCTATTTCTCCCACTATTTATGACGCAG
ATATACGTAGTCGTGGTGTAGGTTATGCCATTGGTTTTGGCCGTATTGGTGCAATTTTATCACCAACAGTGGCAGGTATT
TTTCTCGATAAAGGCTTAGCTCCGTCAACCTTGTATGCCTATTACGGTATTGTTTTTATTTTAGCGATTTTCCTGATTCT
GAGCTTGAGTAAGGCATTTTATCGCCAGAAAGAAGGTCTAGGAAGTTCTATAAAAACAGTTGCTTAA

Upstream 100 bases:

>100_bases
AATTCCTTTTAGGCAATTATGGATATGGATATGCATAATCACCCAGCATTCAAGCTTTGTAATGTCGTGAGTATCTCCTG
TCCAGATTGGGAGATATATA

Downstream 100 bases:

>100_bases
ATCAATATAATAAAACAGTAGAGTTTAAAAAATGAAAAAGACATCCTTATGGGTGGGGGGCTGCTTATGCCTATTATTTA
CCCCTGTAGTCCATGCCGAA

Product: MFS superfamily vanillate transporter

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MQRDILSEINQNNMSRYQWFVIVVCVCLNIIDGFDVMVMAFTAPSVSAEWSLSGAQIGLLLSAGLFGMAAGSIFLAPLAD
KIGRRLLILICLAIAGISMLACGLVHSHSMLAALRFVTGIGVGGILASSNVLASEYANLRWRSLAVSLMSTGYGIGATLG
GILSLLLIEHLGWRSIFLAGGVTTVVMLLVSLWLLPESLDYLLAKKPKQALEKINLTVQRIGLVRLNALPQHEKNTQQHS
SDIAKLFQGKQGFQTLCLWFAFFLVMFGFYFVMSWTPKILIAMGMSPSQGVTTGILISIGGVFGAAIIGLLASRIKIFHA
LSLFLGLTAVCVWLFVAVSSQVSIALMVGLLLGTLINGCVAGLYSISPTIYDADIRSRGVGYAIGFGRIGAILSPTVAGI
FLDKGLAPSTLYAYYGIVFILAIFLILSLSKAFYRQKEGLGSSIKTVA

Sequences:

>Translated_448_residues
MQRDILSEINQNNMSRYQWFVIVVCVCLNIIDGFDVMVMAFTAPSVSAEWSLSGAQIGLLLSAGLFGMAAGSIFLAPLAD
KIGRRLLILICLAIAGISMLACGLVHSHSMLAALRFVTGIGVGGILASSNVLASEYANLRWRSLAVSLMSTGYGIGATLG
GILSLLLIEHLGWRSIFLAGGVTTVVMLLVSLWLLPESLDYLLAKKPKQALEKINLTVQRIGLVRLNALPQHEKNTQQHS
SDIAKLFQGKQGFQTLCLWFAFFLVMFGFYFVMSWTPKILIAMGMSPSQGVTTGILISIGGVFGAAIIGLLASRIKIFHA
LSLFLGLTAVCVWLFVAVSSQVSIALMVGLLLGTLINGCVAGLYSISPTIYDADIRSRGVGYAIGFGRIGAILSPTVAGI
FLDKGLAPSTLYAYYGIVFILAIFLILSLSKAFYRQKEGLGSSIKTVA
>Mature_448_residues
MQRDILSEINQNNMSRYQWFVIVVCVCLNIIDGFDVMVMAFTAPSVSAEWSLSGAQIGLLLSAGLFGMAAGSIFLAPLAD
KIGRRLLILICLAIAGISMLACGLVHSHSMLAALRFVTGIGVGGILASSNVLASEYANLRWRSLAVSLMSTGYGIGATLG
GILSLLLIEHLGWRSIFLAGGVTTVVMLLVSLWLLPESLDYLLAKKPKQALEKINLTVQRIGLVRLNALPQHEKNTQQHS
SDIAKLFQGKQGFQTLCLWFAFFLVMFGFYFVMSWTPKILIAMGMSPSQGVTTGILISIGGVFGAAIIGLLASRIKIFHA
LSLFLGLTAVCVWLFVAVSSQVSIALMVGLLLGTLINGCVAGLYSISPTIYDADIRSRGVGYAIGFGRIGAILSPTVAGI
FLDKGLAPSTLYAYYGIVFILAIFLILSLSKAFYRQKEGLGSSIKTVA

Specific function: Could Be A Transporter For 3-Phenylpropionate (Hydrocinnamic Acid). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=431, Percent_Identity=23.2018561484919, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI213021148, Length=412, Percent_Identity=21.6019417475728, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI7662270, Length=292, Percent_Identity=23.6301369863014, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI87081723, Length=385, Percent_Identity=27.012987012987, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI87082231, Length=213, Percent_Identity=26.7605633802817, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786229, Length=391, Percent_Identity=22.2506393861893, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788068, Length=342, Percent_Identity=23.6842105263158, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI87082404, Length=200, Percent_Identity=24, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788074, Length=352, Percent_Identity=23.5795454545455, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790146, Length=173, Percent_Identity=27.1676300578035, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87082159, Length=392, Percent_Identity=23.2142857142857, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI32564663, Length=418, Percent_Identity=24.1626794258373, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI19922874, Length=389, Percent_Identity=24.6786632390745, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24668456, Length=379, Percent_Identity=24.0105540897098, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47920; Mature: 47920

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRDILSEINQNNMSRYQWFVIVVCVCLNIIDGFDVMVMAFTAPSVSAEWSLSGAQIGLL
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHEEECCCCCCCCCCCCHHHHHH
LSAGLFGMAAGSIFLAPLADKIGRRLLILICLAIAGISMLACGLVHSHSMLAALRFVTGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVGGILASSNVLASEYANLRWRSLAVSLMSTGYGIGATLGGILSLLLIEHLGWRSIFLAG
CHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHC
GVTTVVMLLVSLWLLPESLDYLLAKKPKQALEKINLTVQRIGLVRLNALPQHEKNTQQHS
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
SDIAKLFQGKQGFQTLCLWFAFFLVMFGFYFVMSWTPKILIAMGMSPSQGVTTGILISIG
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCHHHHHHHHH
GVFGAAIIGLLASRIKIFHALSLFLGLTAVCVWLFVAVSSQVSIALMVGLLLGTLINGCV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLYSISPTIYDADIRSRGVGYAIGFGRIGAILSPTVAGIFLDKGLAPSTLYAYYGIVFI
HHHHHCCCCEEECCHHHCCCCEEECHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LAIFLILSLSKAFYRQKEGLGSSIKTVA
HHHHHHHHHHHHHHHHHHCCCCCHHCCC
>Mature Secondary Structure
MQRDILSEINQNNMSRYQWFVIVVCVCLNIIDGFDVMVMAFTAPSVSAEWSLSGAQIGLL
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHEEECCCCCCCCCCCCHHHHHH
LSAGLFGMAAGSIFLAPLADKIGRRLLILICLAIAGISMLACGLVHSHSMLAALRFVTGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVGGILASSNVLASEYANLRWRSLAVSLMSTGYGIGATLGGILSLLLIEHLGWRSIFLAG
CHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHC
GVTTVVMLLVSLWLLPESLDYLLAKKPKQALEKINLTVQRIGLVRLNALPQHEKNTQQHS
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
SDIAKLFQGKQGFQTLCLWFAFFLVMFGFYFVMSWTPKILIAMGMSPSQGVTTGILISIG
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCHHHHHHHHH
GVFGAAIIGLLASRIKIFHALSLFLGLTAVCVWLFVAVSSQVSIALMVGLLLGTLINGCV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLYSISPTIYDADIRSRGVGYAIGFGRIGAILSPTVAGIFLDKGLAPSTLYAYYGIVFI
HHHHHCCCCEEECCHHHCCCCEEECHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LAIFLILSLSKAFYRQKEGLGSSIKTVA
HHHHHHHHHHHHHHHHHHCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8063101 [H]