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The map label for this gene is vanB

Identifier: 50084183

GI number: 50084183

Start: 965539

End: 966495

Strand: Reverse

Name: vanB

Synonym: ACIAD0979

Alternate gene names: 50084183

Gene position: 966495-965539 (Counterclockwise)

Preceding gene: 50084184

Following gene: 50084178

Centisome position: 26.86

GC content: 40.65

Gene sequence:

>957_bases
ATGATAAACATGGACGTGATCATTCACAAAATTCACCAGCTTACTCCAAGTATTCGTGCATTTGAGTTGATCTCTGCCAA
TGGCAGCGACTTACCAGCTTTTGACGCAGGCTCACATATTGACGTACATCTCAAAAATGGATTGACTCGACAATATTCAC
TTTCTAACTGTTGTAGTGAACAACATCGCTATGTGATTGGTGTATTGCACGATGAAAATTCAAGAGGTGGTTCACGTTGT
ATTCATCAGGATTATCGTGAAGGCGATCATTTAAATATTGGTACACCGCGCAATTTATTTGAAATTCATTCTAAAACTCA
GAAAGCAGTTTTATTTGCAGGCGGTATTGGGATTACGCCTATTCTCTCCATGGCTTATCGTCTAAAACATCAGCAAATTC
CATTTGAACTACATTATTTTGTACGTAGTCATGAAATGATTGCATTTTATGGCAACCTGACTGAACACTTCCCAGAGCAA
ATTCATTTTCACATTCAGAATCAGTCTGAAACACAATGTGAAATGTCGAAAGTGCTTGAAGAAGTCGCTCCAGATCGACA
TTTATATGTCTGTGGTCCAGCTGGTTTTATGCAATTTGTGATGGATTCTGCACAGCAGGCAGGTTGGTCAGATGAACAGT
TGCATCAAGAACATTTTGTCGCACCGCAAATCGACCAAAGTCAGAATGAAGCTTTTACCATTGAAGTGCTTGGCTCAGAT
CGTAAAATTGAAGTTTCTGCCCATCAGACGGCGACTCAAGCCTTGCTCGAACATGGCTTTGACGTGCCTGTTTCATGTGA
ACAAGGCATTTGCGGCACCTGTATTACGCGCGTGGTTTCTGGAACACCAGATCATCGTGATGTATTTATGACAGATGAGG
AACATGCTTTAAATGATCAGTTTACACCGTGTTGTTCGCGTGCGAAGTCTAAAATTTTAGTCATTGATCTTGCTTAA

Upstream 100 bases:

>100_bases
TTCAATCACGCAAGATTATTGATCGGTTACTGGCAAAAGAAAATAGCCCGTCACCTCAAGATACACAACGAAAATTTCCC
AATATTCGTGTAATTTAAGG

Downstream 100 bases:

>100_bases
AAGTTCAGTTTTTCTCCTATACATTAAGCCCATGTATAGGAGTTTTTTACTCGGCATCCGCTAAAATCTGACGTACAGTC
TCTCCGAGTACGACAACACT

Product: vanillate O-demethylase oxidoreductase

Products: 3,4-dihydroxybenzoate; NAD+; H2O; formaldehyde

Alternate protein names: Vanillate degradation ferredoxin-like protein

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MINMDVIIHKIHQLTPSIRAFELISANGSDLPAFDAGSHIDVHLKNGLTRQYSLSNCCSEQHRYVIGVLHDENSRGGSRC
IHQDYREGDHLNIGTPRNLFEIHSKTQKAVLFAGGIGITPILSMAYRLKHQQIPFELHYFVRSHEMIAFYGNLTEHFPEQ
IHFHIQNQSETQCEMSKVLEEVAPDRHLYVCGPAGFMQFVMDSAQQAGWSDEQLHQEHFVAPQIDQSQNEAFTIEVLGSD
RKIEVSAHQTATQALLEHGFDVPVSCEQGICGTCITRVVSGTPDHRDVFMTDEEHALNDQFTPCCSRAKSKILVIDLA

Sequences:

>Translated_318_residues
MINMDVIIHKIHQLTPSIRAFELISANGSDLPAFDAGSHIDVHLKNGLTRQYSLSNCCSEQHRYVIGVLHDENSRGGSRC
IHQDYREGDHLNIGTPRNLFEIHSKTQKAVLFAGGIGITPILSMAYRLKHQQIPFELHYFVRSHEMIAFYGNLTEHFPEQ
IHFHIQNQSETQCEMSKVLEEVAPDRHLYVCGPAGFMQFVMDSAQQAGWSDEQLHQEHFVAPQIDQSQNEAFTIEVLGSD
RKIEVSAHQTATQALLEHGFDVPVSCEQGICGTCITRVVSGTPDHRDVFMTDEEHALNDQFTPCCSRAKSKILVIDLA
>Mature_318_residues
MINMDVIIHKIHQLTPSIRAFELISANGSDLPAFDAGSHIDVHLKNGLTRQYSLSNCCSEQHRYVIGVLHDENSRGGSRC
IHQDYREGDHLNIGTPRNLFEIHSKTQKAVLFAGGIGITPILSMAYRLKHQQIPFELHYFVRSHEMIAFYGNLTEHFPEQ
IHFHIQNQSETQCEMSKVLEEVAPDRHLYVCGPAGFMQFVMDSAQQAGWSDEQLHQEHFVAPQIDQSQNEAFTIEVLGSD
RKIEVSAHQTATQALLEHGFDVPVSCEQGICGTCITRVVSGTPDHRDVFMTDEEHALNDQFTPCCSRAKSKILVIDLA

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain

Homologues:

Organism=Escherichia coli, GI1788104, Length=319, Percent_Identity=36.3636363636364, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1787098, Length=282, Percent_Identity=24.822695035461, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1787658, Length=234, Percent_Identity=25.6410256410256, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): VANB_ACIAD (O24840)

Other databases:

- EMBL:   AF009672
- EMBL:   CR543861
- RefSeq:   YP_045693.1
- ProteinModelPortal:   O24840
- SMR:   O24840
- STRING:   O24840
- GeneID:   2879214
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0979
- NMPDR:   fig|62977.3.peg.1000
- eggNOG:   COG1018
- HOGENOM:   HBG483999
- OMA:   HYCARSS
- ProtClustDB:   CLSK707148
- BioCyc:   ASP62977:ACIAD0979-MONOMER
- GO:   GO:0006810
- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR00409

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1; SSF54292 Ferredoxin; SSF63380 Riboflavin_synthase_like_b-brl

EC number: 1.14.13.82

Molecular weight: Translated: 35817; Mature: 35817

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00197 2FE2S_FER_1; PS51085 2FE2S_FER_2; PS51384 FAD_FR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINMDVIIHKIHQLTPSIRAFELISANGSDLPAFDAGSHIDVHLKNGLTRQYSLSNCCSE
CCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
QHRYVIGVLHDENSRGGSRCIHQDYREGDHLNIGTPRNLFEIHSKTQKAVLFAGGIGITP
CCCEEEEEEECCCCCCCHHHHHHHHCCCCCEECCCCHHHHHHHHCCCEEEEEECCCCHHH
ILSMAYRLKHQQIPFELHYFVRSHEMIAFYGNLTEHFPEQIHFHIQNQSETQCEMSKVLE
HHHHHHHHHHHCCCHHEEEEHHCCCEEEEECCHHHHCCHHEEEEECCCCHHHHHHHHHHH
EVAPDRHLYVCGPAGFMQFVMDSAQQAGWSDEQLHQEHFVAPQIDQSQNEAFTIEVLGSD
HHCCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCC
RKIEVSAHQTATQALLEHGFDVPVSCEQGICGTCITRVVSGTPDHRDVFMTDEEHALNDQ
CEEEEHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCHHHCCCC
FTPCCSRAKSKILVIDLA
CCHHHHHCCCEEEEEEEC
>Mature Secondary Structure
MINMDVIIHKIHQLTPSIRAFELISANGSDLPAFDAGSHIDVHLKNGLTRQYSLSNCCSE
CCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
QHRYVIGVLHDENSRGGSRCIHQDYREGDHLNIGTPRNLFEIHSKTQKAVLFAGGIGITP
CCCEEEEEEECCCCCCCHHHHHHHHCCCCCEECCCCHHHHHHHHCCCEEEEEECCCCHHH
ILSMAYRLKHQQIPFELHYFVRSHEMIAFYGNLTEHFPEQIHFHIQNQSETQCEMSKVLE
HHHHHHHHHHHCCCHHEEEEHHCCCEEEEECCHHHHCCHHEEEEECCCCHHHHHHHHHHH
EVAPDRHLYVCGPAGFMQFVMDSAQQAGWSDEQLHQEHFVAPQIDQSQNEAFTIEVLGSD
HHCCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCC
RKIEVSAHQTATQALLEHGFDVPVSCEQGICGTCITRVVSGTPDHRDVFMTDEEHALNDQ
CEEEEHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCHHHCCCC
FTPCCSRAKSKILVIDLA
CCHHHHHCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vanillate; O2; NADH; H+

Specific reaction: vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10348863