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The map label for this gene is iciA [H]

Identifier: 50084172

GI number: 50084172

Start: 954585

End: 955478

Strand: Direct

Name: iciA [H]

Synonym: ACIAD0967

Alternate gene names: 50084172

Gene position: 954585-955478 (Clockwise)

Preceding gene: 50084170

Following gene: 50084173

Centisome position: 26.53

GC content: 41.61

Gene sequence:

>894_bases
ATGCTTGATAATAAACAATGTGATGCCTTTTTTGCTGTTGCAGAACAGGGAAGCTTTGAGCAAGCAGCCATTCAATTGTG
CATTACTGCTTCGGCTGTGACATTGCGTGTACAAGCCCTTGAAAAAGAGCTGGGGCAGTTATTACTGATTCGAGCAAGAC
CGTGTACTTTGACACATGCAGGTCAGCAATTATTTGAACATTTACAGCATACTAGACGCCTTGAACAAAATTTTCTGCAC
AATTTGACTGACGCAACGCCATCACAATTTTTTAAAGTTATGATGGCAGCAAATGCCGATTCATTGGCCACTTGGCTTTT
ACCTGCTTTAAATGAAGTGGCTTCTCAACAAAAGTTGATGTTAGAAATCTTACTAGATGATCAGTCACATACTCATGCTT
TAATGGAAAAGGGAACGGTGAGTGCTTGCATATCCATCGAACCCAAAGCTATGAAAGGATGCAAGTCGGTTTATTTGGGA
AGTATGCGTTATAAGATGCTGGCGACGCAGGCATTTCAAGGTACATGGTTTCGTCATGGCGTACATCGTGAAGCGCTACG
GGTTGCACCCGCTATTATTTTTAATCATAAAGATCAGATGCATTTTGATCTGCTCGAAAAACAATATGGACTTATTAAGG
GGGCTTATCCCTATCACTATATTCCATCTTCAGCGTGTTTTTTTACAGCAGTGCAGTTGGGTTTAGGGTATGGTTTGGTG
CCTGAATTACAATTAAAACAAGCAGCAGATCCCGATCAATTACGAGATATTATGCCTGAGATGCAGCTGGATATACCATT
GTACTGGCATCATTGGCAGCAGCAATCTCGACCACTGCAATCTCTCACAGAACATTTAACCCAGCATGCAGCTAAATATC
TGAGCCCATTATGA

Upstream 100 bases:

>100_bases
TAAATGAGTTCAAATCAATCACAATGATTATCTACTTTTCATTATTGAAATCAGTAAAATTAAGAATATATAAGCTGAAC
TAAATTTTTTTAAGGAAACT

Downstream 100 bases:

>100_bases
TTATGTTGGGTTTACATCTATTTGACTGCGATGAAAGGTTTGTAAATCTTAAAAACGCACTATAATGCAGGCTTATATTT
ATCGTATTTGATTATACTTA

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MLDNKQCDAFFAVAEQGSFEQAAIQLCITASAVTLRVQALEKELGQLLLIRARPCTLTHAGQQLFEHLQHTRRLEQNFLH
NLTDATPSQFFKVMMAANADSLATWLLPALNEVASQQKLMLEILLDDQSHTHALMEKGTVSACISIEPKAMKGCKSVYLG
SMRYKMLATQAFQGTWFRHGVHREALRVAPAIIFNHKDQMHFDLLEKQYGLIKGAYPYHYIPSSACFFTAVQLGLGYGLV
PELQLKQAADPDQLRDIMPEMQLDIPLYWHHWQQQSRPLQSLTEHLTQHAAKYLSPL

Sequences:

>Translated_297_residues
MLDNKQCDAFFAVAEQGSFEQAAIQLCITASAVTLRVQALEKELGQLLLIRARPCTLTHAGQQLFEHLQHTRRLEQNFLH
NLTDATPSQFFKVMMAANADSLATWLLPALNEVASQQKLMLEILLDDQSHTHALMEKGTVSACISIEPKAMKGCKSVYLG
SMRYKMLATQAFQGTWFRHGVHREALRVAPAIIFNHKDQMHFDLLEKQYGLIKGAYPYHYIPSSACFFTAVQLGLGYGLV
PELQLKQAADPDQLRDIMPEMQLDIPLYWHHWQQQSRPLQSLTEHLTQHAAKYLSPL
>Mature_297_residues
MLDNKQCDAFFAVAEQGSFEQAAIQLCITASAVTLRVQALEKELGQLLLIRARPCTLTHAGQQLFEHLQHTRRLEQNFLH
NLTDATPSQFFKVMMAANADSLATWLLPALNEVASQQKLMLEILLDDQSHTHALMEKGTVSACISIEPKAMKGCKSVYLG
SMRYKMLATQAFQGTWFRHGVHREALRVAPAIIFNHKDQMHFDLLEKQYGLIKGAYPYHYIPSSACFFTAVQLGLGYGLV
PELQLKQAADPDQLRDIMPEMQLDIPLYWHHWQQQSRPLQSLTEHLTQHAAKYLSPL

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=292, Percent_Identity=34.2465753424658, Blast_Score=175, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33669; Mature: 33669

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDNKQCDAFFAVAEQGSFEQAAIQLCITASAVTLRVQALEKELGQLLLIRARPCTLTHA
CCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHH
GQQLFEHLQHTRRLEQNFLHNLTDATPSQFFKVMMAANADSLATWLLPALNEVASQQKLM
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LEILLDDQSHTHALMEKGTVSACISIEPKAMKGCKSVYLGSMRYKMLATQAFQGTWFRHG
HHHHHCCCCHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
VHREALRVAPAIIFNHKDQMHFDLLEKQYGLIKGAYPYHYIPSSACFFTAVQLGLGYGLV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCC
PELQLKQAADPDQLRDIMPEMQLDIPLYWHHWQQQSRPLQSLTEHLTQHAAKYLSPL
CCHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDNKQCDAFFAVAEQGSFEQAAIQLCITASAVTLRVQALEKELGQLLLIRARPCTLTHA
CCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHH
GQQLFEHLQHTRRLEQNFLHNLTDATPSQFFKVMMAANADSLATWLLPALNEVASQQKLM
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LEILLDDQSHTHALMEKGTVSACISIEPKAMKGCKSVYLGSMRYKMLATQAFQGTWFRHG
HHHHHCCCCHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
VHREALRVAPAIIFNHKDQMHFDLLEKQYGLIKGAYPYHYIPSSACFFTAVQLGLGYGLV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCC
PELQLKQAADPDQLRDIMPEMQLDIPLYWHHWQQQSRPLQSLTEHLTQHAAKYLSPL
CCHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA