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The map label for this gene is glpK

Identifier: 50084137

GI number: 50084137

Start: 913230

End: 914717

Strand: Reverse

Name: glpK

Synonym: ACIAD0930

Alternate gene names: 50084137

Gene position: 914717-913230 (Counterclockwise)

Preceding gene: 50084138

Following gene: 50084133

Centisome position: 25.42

GC content: 44.62

Gene sequence:

>1488_bases
ATGAGCTACTTACTTGCCCTCGATCAAGGAACGACATCTAGCCGCGCCATTATCTTCGATGAAAAAGGCCAGATCCATGC
AACTGCACAACGTGAAACACGTATCAAAACCCCAAATTCTGGTTGGGTAGAACAAGATGCCAATGAAATATGGTCAAGCC
AGATTGCTGTTATTCAGCAAGCCTTGGCTTCAGCTCATATTCTGGCAAAGGATATCAAGGCTTTAGGACTGACCAATCAA
CGTGAAACAACAGTCGTTTGGGATAAACGCACAGGTAAAGCACTTGCGCCCGCAATTATCTGGCAAGATCGACGTGCAGC
ACAGTGGTGTAATACCTTAATTGAAAATGGCATGCTGGTACAGGTTCAGCAAAAAACAGGTTTAAGAATTGACCCCTATT
TTAGTGCAGGAAAATTGGTCTGGTTACTTGAAAATATTGCGGGATTTCGAATACTTGCCGAACAGGGACATGCCGCGTTT
GGTACCATTGATAGTTGGCTGGTCTGGAATTTAACTCAAGGTGCAGAGCATATCATTGAGGCCAGTAATGCGTCTCGTAC
CATGCTCATGAACCTTTCAACACAAATGTGGGATGAAGATTTACTTAATAAATTTAATATTCCAGCAGCAATTCTACCTA
AAATCATTAGCTCAGATGCATATGTGGCCGATACTGCACAAGGTCTTTTGGGAAGTACAATTCCGATTACTGGAATATTG
GGTGATCAACAAGCAGCGTTATTTGGTCAGTCATGTTTTGAGGTAGGTTCTGCCAAAAACACCTATGGCACAGGATGTTT
TATGCTGTTCAATACAGGAGATCAACTTCAATTTAGTCAAAATCAGTTGCTCACTACCCTTGCATGGCAATGTCAAAATC
AAACCCGTTATGCACTCGAAGGCAGTGTATTTATGGCAGGCGCCATTGTGCAATGGTTACGTGATGGTCTTGGACTCATT
CAGCATAGTGCCCAAGTTGAGCAACTTGCCAGTCAGGTGCAAAGTACAGAAGGTGTCGTTTTGGTGCCCGCATTTACTGG
GCTTGGTGCGCCGCACTGGGACAGTGAAGCGCGTGCTTTGCTTTGTGGAATGTCGCGCGGTACCACAAAAGCACATATTG
CTCGTGCAGCACTTGAAGCGATTGCCTTCCAAGTCTCTGATGTGTTGTGTGCCATGCAGTCTGATTTATCGCGGCCATTA
AAAGAACTTCGTGTCGATGGGGGTGCCAGCCAAAACGATATGCTCATGCAATTTCAGGCCGATATTTTAAATGTACCTGT
TCTACGGCCTAAAATGCTTGAATCAACCGCTTGGGGTGCAGCAGCCATGGCCGGCTTAAAAGCAGGTGTTTTTACCAATC
TGGATGAAATTGCAGCGTCATGGCAACTTGATCGTACTTTTGAGCCAAAGATGAAAAATGACGAACGTGAATCACGTTTG
TGTGAATGGAGTCAGGCACTCAAAAGAGCGAAGTCCAATTTGATATAA

Upstream 100 bases:

>100_bases
AAAATTGCTATGCAATTTATAACCAAATGAACTGCGCCAATGTATTTAACATGATAATTGAGCTACTATTTTATTTTTCA
AGGATGCCTAATAAGAAAAC

Downstream 100 bases:

>100_bases
ATAAAAAAGCTGCAATAACTTGCAGCTTTTTTATTGATCTAAATTAAATGCCTGAAACTATAAAGCACCATTACCCAGTA
CAATACCTTCACGTCGAGGG

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSYLLALDQGTTSSRAIIFDEKGQIHATAQRETRIKTPNSGWVEQDANEIWSSQIAVIQQALASAHILAKDIKALGLTNQ
RETTVVWDKRTGKALAPAIIWQDRRAAQWCNTLIENGMLVQVQQKTGLRIDPYFSAGKLVWLLENIAGFRILAEQGHAAF
GTIDSWLVWNLTQGAEHIIEASNASRTMLMNLSTQMWDEDLLNKFNIPAAILPKIISSDAYVADTAQGLLGSTIPITGIL
GDQQAALFGQSCFEVGSAKNTYGTGCFMLFNTGDQLQFSQNQLLTTLAWQCQNQTRYALEGSVFMAGAIVQWLRDGLGLI
QHSAQVEQLASQVQSTEGVVLVPAFTGLGAPHWDSEARALLCGMSRGTTKAHIARAALEAIAFQVSDVLCAMQSDLSRPL
KELRVDGGASQNDMLMQFQADILNVPVLRPKMLESTAWGAAAMAGLKAGVFTNLDEIAASWQLDRTFEPKMKNDERESRL
CEWSQALKRAKSNLI

Sequences:

>Translated_495_residues
MSYLLALDQGTTSSRAIIFDEKGQIHATAQRETRIKTPNSGWVEQDANEIWSSQIAVIQQALASAHILAKDIKALGLTNQ
RETTVVWDKRTGKALAPAIIWQDRRAAQWCNTLIENGMLVQVQQKTGLRIDPYFSAGKLVWLLENIAGFRILAEQGHAAF
GTIDSWLVWNLTQGAEHIIEASNASRTMLMNLSTQMWDEDLLNKFNIPAAILPKIISSDAYVADTAQGLLGSTIPITGIL
GDQQAALFGQSCFEVGSAKNTYGTGCFMLFNTGDQLQFSQNQLLTTLAWQCQNQTRYALEGSVFMAGAIVQWLRDGLGLI
QHSAQVEQLASQVQSTEGVVLVPAFTGLGAPHWDSEARALLCGMSRGTTKAHIARAALEAIAFQVSDVLCAMQSDLSRPL
KELRVDGGASQNDMLMQFQADILNVPVLRPKMLESTAWGAAAMAGLKAGVFTNLDEIAASWQLDRTFEPKMKNDERESRL
CEWSQALKRAKSNLI
>Mature_494_residues
SYLLALDQGTTSSRAIIFDEKGQIHATAQRETRIKTPNSGWVEQDANEIWSSQIAVIQQALASAHILAKDIKALGLTNQR
ETTVVWDKRTGKALAPAIIWQDRRAAQWCNTLIENGMLVQVQQKTGLRIDPYFSAGKLVWLLENIAGFRILAEQGHAAFG
TIDSWLVWNLTQGAEHIIEASNASRTMLMNLSTQMWDEDLLNKFNIPAAILPKIISSDAYVADTAQGLLGSTIPITGILG
DQQAALFGQSCFEVGSAKNTYGTGCFMLFNTGDQLQFSQNQLLTTLAWQCQNQTRYALEGSVFMAGAIVQWLRDGLGLIQ
HSAQVEQLASQVQSTEGVVLVPAFTGLGAPHWDSEARALLCGMSRGTTKAHIARAALEAIAFQVSDVLCAMQSDLSRPLK
ELRVDGGASQNDMLMQFQADILNVPVLRPKMLESTAWGAAAMAGLKAGVFTNLDEIAASWQLDRTFEPKMKNDERESRLC
EWSQALKRAKSNLI

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=498, Percent_Identity=44.9799196787149, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI4504007, Length=498, Percent_Identity=44.9799196787149, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI42794763, Length=503, Percent_Identity=44.3339960238569, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI41393575, Length=497, Percent_Identity=45.4728370221328, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI88196792, Length=506, Percent_Identity=31.0276679841897, Blast_Score=291, Evalue=9e-79,
Organism=Escherichia coli, GI1790361, Length=490, Percent_Identity=50.4081632653061, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI1789987, Length=477, Percent_Identity=24.1090146750524, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1790005, Length=488, Percent_Identity=23.9754098360656, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787789, Length=513, Percent_Identity=27.2904483430799, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789168, Length=490, Percent_Identity=24.2857142857143, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17535599, Length=501, Percent_Identity=39.9201596806387, Blast_Score=347, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI71986140, Length=523, Percent_Identity=26.0038240917782, Blast_Score=177, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI71986147, Length=265, Percent_Identity=31.3207547169811, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321755, Length=497, Percent_Identity=37.625754527163, Blast_Score=298, Evalue=2e-81,
Organism=Drosophila melanogaster, GI17864214, Length=506, Percent_Identity=43.4782608695652, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24654573, Length=432, Percent_Identity=45.3703703703704, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655555, Length=501, Percent_Identity=39.9201596806387, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24655560, Length=501, Percent_Identity=39.9201596806387, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24655570, Length=501, Percent_Identity=39.9201596806387, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24655574, Length=501, Percent_Identity=39.9201596806387, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24655565, Length=501, Percent_Identity=39.9201596806387, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI19922058, Length=524, Percent_Identity=31.6793893129771, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI21357867, Length=522, Percent_Identity=31.0344827586207, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24656367, Length=510, Percent_Identity=31.3725490196078, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24652871, Length=491, Percent_Identity=32.1792260692464, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24656372, Length=386, Percent_Identity=31.8652849740933, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI45552909, Length=331, Percent_Identity=29.3051359516616, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_ACIAD (Q6FDN3)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045647.1
- HSSP:   P0A6F3
- ProteinModelPortal:   Q6FDN3
- SMR:   Q6FDN3
- STRING:   Q6FDN3
- GeneID:   2879886
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0930
- NMPDR:   fig|62977.3.peg.929
- eggNOG:   COG0554
- HOGENOM:   HBG511469
- OMA:   KPSSEVY
- PhylomeDB:   Q6FDN3
- BioCyc:   ASP62977:ACIAD0930-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 54272; Mature: 54140

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 11-11 BINDING 15-15 BINDING 81-81 BINDING 133-133 BINDING 242-242 BINDING 264-264 BINDING 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYLLALDQGTTSSRAIIFDEKGQIHATAQRETRIKTPNSGWVEQDANEIWSSQIAVIQQ
CCEEEEECCCCCCCEEEEEECCCCEEEEECCCCEECCCCCCCCCCHHHHHHHHHHHHHHH
ALASAHILAKDIKALGLTNQRETTVVWDKRTGKALAPAIIWQDRRAAQWCNTLIENGMLV
HHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHCHHHHCCCCHHHHHHHHHHCCCEEE
QVQQKTGLRIDPYFSAGKLVWLLENIAGFRILAEQGHAAFGTIDSWLVWNLTQGAEHIIE
EEECCCCCEECCCCCCCHHHHHHHHHCCEEEEECCCCCEECCCCCHHEEEEHHHHHHHHH
ASNASRTMLMNLSTQMWDEDLLNKFNIPAAILPKIISSDAYVADTAQGLLGSTIPITGIL
CCCCCCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHCCCCEEHHHHHHHCCCCCCEEEEE
GDQQAALFGQSCFEVGSAKNTYGTGCFMLFNTGDQLQFSQNQLLTTLAWQCQNQTRYALE
CCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEECHHHHHHHHHHHHCCCCEEEEC
GSVFMAGAIVQWLRDGLGLIQHSAQVEQLASQVQSTEGVVLVPAFTGLGAPHWDSEARAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHEE
LCGMSRGTTKAHIARAALEAIAFQVSDVLCAMQSDLSRPLKELRVDGGASQNDMLMQFQA
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
DILNVPVLRPKMLESTAWGAAAMAGLKAGVFTNLDEIAASWQLDRTFEPKMKNDERESRL
HHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCHHHHHH
CEWSQALKRAKSNLI
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
SYLLALDQGTTSSRAIIFDEKGQIHATAQRETRIKTPNSGWVEQDANEIWSSQIAVIQQ
CEEEEECCCCCCCEEEEEECCCCEEEEECCCCEECCCCCCCCCCHHHHHHHHHHHHHHH
ALASAHILAKDIKALGLTNQRETTVVWDKRTGKALAPAIIWQDRRAAQWCNTLIENGMLV
HHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHCHHHHCCCCHHHHHHHHHHCCCEEE
QVQQKTGLRIDPYFSAGKLVWLLENIAGFRILAEQGHAAFGTIDSWLVWNLTQGAEHIIE
EEECCCCCEECCCCCCCHHHHHHHHHCCEEEEECCCCCEECCCCCHHEEEEHHHHHHHHH
ASNASRTMLMNLSTQMWDEDLLNKFNIPAAILPKIISSDAYVADTAQGLLGSTIPITGIL
CCCCCCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHCCCCEEHHHHHHHCCCCCCEEEEE
GDQQAALFGQSCFEVGSAKNTYGTGCFMLFNTGDQLQFSQNQLLTTLAWQCQNQTRYALE
CCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEECHHHHHHHHHHHHCCCCEEEEC
GSVFMAGAIVQWLRDGLGLIQHSAQVEQLASQVQSTEGVVLVPAFTGLGAPHWDSEARAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHEE
LCGMSRGTTKAHIARAALEAIAFQVSDVLCAMQSDLSRPLKELRVDGGASQNDMLMQFQA
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
DILNVPVLRPKMLESTAWGAAAMAGLKAGVFTNLDEIAASWQLDRTFEPKMKNDERESRL
HHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCHHHHHH
CEWSQALKRAKSNLI
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA