Click here to switch to the map view.

The map label for this gene is hemB [H]

Identifier: 50084131

GI number: 50084131

Start: 906369

End: 907430

Strand: Reverse

Name: hemB [H]

Synonym: ACIAD0923

Alternate gene names: 50084131

Gene position: 907430-906369 (Counterclockwise)

Preceding gene: 50084133

Following gene: 50084130

Centisome position: 25.22

GC content: 44.82

Gene sequence:

>1062_bases
ATGGGTCTACAATACCCCACAAGTTTAATACTGAACATTGAGATTGTTATGACTTATACGTTCAATCGTCCTGCTTTTCC
CGCAACGCGTATGCGCCGTATCCGTAAAAATGATCAATTGCGAGCAATGGTCAGTGAAACACAACTGACGCCTCATCATC
TCATTTATCCAGTATTTGTATTGCCAGGGCAAAACCAGCGCCAGGATATTCCAAGCATGCCGAATGTTCAGCGCCTATCT
GCCGATCTGTTGCTCAAAAAGGCTGAACAGCTTTTAGAATTGGGTGTATCAAAACTGGCTCTATTTCCCGTGACTCCACA
AGAAGATAAAAGTCTAAGCGCCGAAGCGGCATGGCGTGAAGATGGATTGGTCCAGGAAACCTGTCGTCTGCTTAAAAAAG
AATTGCCAGAAATGGTACTGATCACCGATGGTGCTTTAGATCCTTATACCACGCATGGTCAGGATGGCATTATCGATGAC
ACAGGTTATGTGCTCAATGATGAAACGGTTGAATGTCTGGTGAAGCAAGCACTAAGCCATGCTGCAGCTGGCGCAGATGT
GATTGCACCAAGTGATATGATGGATGGGCGTATTGGTGCGATTCGTCAGGCATTAGAGCAAAATAATTTCATTTATACCA
ATATCATGGCCTATTCTGCAAAATATGCTTCTAGCTTTTACGGTCCATTCCGCGACGCAGTTGGCTCGGCAACCAACCTT
AAAGGTGGTAATAAGTATAACTACCAGATGGATATCGGAAATCGTGCCGAAGCACTCCACGAAATTGCCCTTGATCTTCA
GGAAGGTGCAGATATGGTCATTGTCAAACCTGGTATGCCGTATCTGGATGTCGTTCGTGAAGTGAAAGATACTTTTGGTG
TTCCGACCTTCATCTATCAAGTCAGTGGTGAATATGCCATGTTGGCAGGTGCCATCCAGAATGGATGGCTATCTGACAGT
GTGATTTTAGAATCACTGATGTGCTGTCGTCGTGCGGGTGCAGACGGTATTTGGACATATTTTGCAGAAGAAGCCGCACA
AAAACTCAAAGAGATGAAATAA

Upstream 100 bases:

>100_bases
ATGTATATGCCCAGTCCACAGTTTTTTTGATTCAGTCATAAAATACTCAACGTTGATAAATCTGCATTAAAGCCAATTCT
GCTTGTACAAATGCCCAGTG

Downstream 100 bases:

>100_bases
GCAGAATACACATCGGATATGAGCATGCATATTGCCATTATTGGCGCGGGCGTAAGTGGTTTACTCACCGCACTGGAGTT
GATTGAACACGGTTGTCAGG

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MGLQYPTSLILNIEIVMTYTFNRPAFPATRMRRIRKNDQLRAMVSETQLTPHHLIYPVFVLPGQNQRQDIPSMPNVQRLS
ADLLLKKAEQLLELGVSKLALFPVTPQEDKSLSAEAAWREDGLVQETCRLLKKELPEMVLITDGALDPYTTHGQDGIIDD
TGYVLNDETVECLVKQALSHAAAGADVIAPSDMMDGRIGAIRQALEQNNFIYTNIMAYSAKYASSFYGPFRDAVGSATNL
KGGNKYNYQMDIGNRAEALHEIALDLQEGADMVIVKPGMPYLDVVREVKDTFGVPTFIYQVSGEYAMLAGAIQNGWLSDS
VILESLMCCRRAGADGIWTYFAEEAAQKLKEMK

Sequences:

>Translated_353_residues
MGLQYPTSLILNIEIVMTYTFNRPAFPATRMRRIRKNDQLRAMVSETQLTPHHLIYPVFVLPGQNQRQDIPSMPNVQRLS
ADLLLKKAEQLLELGVSKLALFPVTPQEDKSLSAEAAWREDGLVQETCRLLKKELPEMVLITDGALDPYTTHGQDGIIDD
TGYVLNDETVECLVKQALSHAAAGADVIAPSDMMDGRIGAIRQALEQNNFIYTNIMAYSAKYASSFYGPFRDAVGSATNL
KGGNKYNYQMDIGNRAEALHEIALDLQEGADMVIVKPGMPYLDVVREVKDTFGVPTFIYQVSGEYAMLAGAIQNGWLSDS
VILESLMCCRRAGADGIWTYFAEEAAQKLKEMK
>Mature_352_residues
GLQYPTSLILNIEIVMTYTFNRPAFPATRMRRIRKNDQLRAMVSETQLTPHHLIYPVFVLPGQNQRQDIPSMPNVQRLSA
DLLLKKAEQLLELGVSKLALFPVTPQEDKSLSAEAAWREDGLVQETCRLLKKELPEMVLITDGALDPYTTHGQDGIIDDT
GYVLNDETVECLVKQALSHAAAGADVIAPSDMMDGRIGAIRQALEQNNFIYTNIMAYSAKYASSFYGPFRDAVGSATNLK
GGNKYNYQMDIGNRAEALHEIALDLQEGADMVIVKPGMPYLDVVREVKDTFGVPTFIYQVSGEYAMLAGAIQNGWLSDSV
ILESLMCCRRAGADGIWTYFAEEAAQKLKEMK

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=316, Percent_Identity=41.7721518987342, Blast_Score=224, Evalue=1e-58,
Organism=Escherichia coli, GI87081728, Length=324, Percent_Identity=46.6049382716049, Blast_Score=251, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6321398, Length=317, Percent_Identity=36.2776025236593, Blast_Score=182, Evalue=8e-47,
Organism=Drosophila melanogaster, GI21358291, Length=300, Percent_Identity=40.6666666666667, Blast_Score=218, Evalue=6e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 39226; Mature: 39094

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLQYPTSLILNIEIVMTYTFNRPAFPATRMRRIRKNDQLRAMVSETQLTPHHLIYPVFV
CCCCCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEEEE
LPGQNQRQDIPSMPNVQRLSADLLLKKAEQLLELGVSKLALFPVTPQEDKSLSAEAAWRE
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHEEEECCCCCCCCCCCHHHHHHC
DGLVQETCRLLKKELPEMVLITDGALDPYTTHGQDGIIDDTGYVLNDETVECLVKQALSH
CCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHH
AAAGADVIAPSDMMDGRIGAIRQALEQNNFIYTNIMAYSAKYASSFYGPFRDAVGSATNL
HHCCCCCCCCCHHCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCHHHHHCCCCCCC
KGGNKYNYQMDIGNRAEALHEIALDLQEGADMVIVKPGMPYLDVVREVKDTFGVPTFIYQ
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEE
VSGEYAMLAGAIQNGWLSDSVILESLMCCRRAGADGIWTYFAEEAAQKLKEMK
ECCCCEEEHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GLQYPTSLILNIEIVMTYTFNRPAFPATRMRRIRKNDQLRAMVSETQLTPHHLIYPVFV
CCCCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEEEE
LPGQNQRQDIPSMPNVQRLSADLLLKKAEQLLELGVSKLALFPVTPQEDKSLSAEAAWRE
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHEEEECCCCCCCCCCCHHHHHHC
DGLVQETCRLLKKELPEMVLITDGALDPYTTHGQDGIIDDTGYVLNDETVECLVKQALSH
CCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHH
AAAGADVIAPSDMMDGRIGAIRQALEQNNFIYTNIMAYSAKYASSFYGPFRDAVGSATNL
HHCCCCCCCCCHHCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCHHHHHCCCCCCC
KGGNKYNYQMDIGNRAEALHEIALDLQEGADMVIVKPGMPYLDVVREVKDTFGVPTFIYQ
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEE
VSGEYAMLAGAIQNGWLSDSVILESLMCCRRAGADGIWTYFAEEAAQKLKEMK
ECCCCEEEHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9529530; 10984043; 10356331; 12079382 [H]