Click here to switch to the map view.

The map label for this gene is dadA [C]

Identifier: 50084130

GI number: 50084130

Start: 905229

End: 906344

Strand: Reverse

Name: dadA [C]

Synonym: ACIAD0922

Alternate gene names: 50084130

Gene position: 906344-905229 (Counterclockwise)

Preceding gene: 50084131

Following gene: 50084127

Centisome position: 25.19

GC content: 45.52

Gene sequence:

>1116_bases
ATGCATATTGCCATTATTGGCGCGGGCGTAAGTGGTTTACTCACCGCACTGGAGTTGATTGAACACGGTTGTCAGGTCTC
TATTTTTGATCAGCAACAGGCAGGACAAGCCGCCTCGTGGGCAGGTGGTGGTATTTTATCGCCTATGTATCCATGGCGCT
ATCCGCAAGCGGTCAATACACTCGCCCAAAATGGCAAAACCCTGTATCAACAATGGAACCAAAAATTGCAGCCGCTGACA
GGTATCGATTTTGAAATCCACGATACAGGCATGCTGATTTTTGATGAAAGCGATTTTGAAATTGGCTTAAATTACGCAAA
AAAGCATCGAGAGCCGATGCAACACGCCAGCTATCTGGATGGCAACCCACTGCAACAGGTCAATTCACGGATCAATTCCA
AATTGGAACAGGCGATCTATTTTCCTGAACTCTCCAATATCCGTAATCCACGCCTTTTACAATCCATCATGGCTTATTTA
AAACAGCATCCTTTAGTTCGCTTTTTTGAACATCGTCCCGTGGTCAAACTTCATATTCAGCAGCATCAAATTGGGTATGT
ACTTGATGAATCAGGCATAAAAATTGAAGCGGATCAGTTTGTGATTGCAACTGGCGCATGGAGCCAGCACTGGTCACAAC
AGCTTGATCTGGATATTCCTGTACATCCAGTACAAGGACAAATGGTACTGTTTAAAGCCCCTGCCCAATGGCTACCGACC
ATGTGCATGAATAACGTGATGTATCTGATACCACGTCAGGATGGCCATATTGTCTGCGGTTCCAGTATGGCACATTGTGG
TTTTAACACCCTACCAGACTCAACCACCAAGCAAAATATTGTTGATGCCTGTCTGGAAATGGTGCCTGAATTGGCTCAAT
TTCCAATTGTAAAACAATGGGCTGGACTTCGACCAAGCTCACCTACAGGTATTCCCTATATTGGCCAAGTGCCAGAAATA
GATAACTTATGGATCAATGCGGGGCATTTCCGTAATGGGTTATGTATGGGGCCTGCCTCTGCACAACTGTTACGTCAGCT
CATGCTCAAACAACCCACAGACATAAACCCAGCCCTTTATTCTCCCGAACGCCTCAGTAAGCCTGTCATGGTATAG

Upstream 100 bases:

>100_bases
TCGTCGTGCGGGTGCAGACGGTATTTGGACATATTTTGCAGAAGAAGCCGCACAAAAACTCAAAGAGATGAAATAAGCAG
AATACACATCGGATATGAGC

Downstream 100 bases:

>100_bases
CACTAAGATTTAGAATAAATCTGGCGTAATGCTTGATCGAGATGAGCAAACTGAAAAAGATAACCTGCGTCTAGCAACGC
TTTAGGTTGCACATACTGTC

Product: D-amino acid oxidase

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MHIAIIGAGVSGLLTALELIEHGCQVSIFDQQQAGQAASWAGGGILSPMYPWRYPQAVNTLAQNGKTLYQQWNQKLQPLT
GIDFEIHDTGMLIFDESDFEIGLNYAKKHREPMQHASYLDGNPLQQVNSRINSKLEQAIYFPELSNIRNPRLLQSIMAYL
KQHPLVRFFEHRPVVKLHIQQHQIGYVLDESGIKIEADQFVIATGAWSQHWSQQLDLDIPVHPVQGQMVLFKAPAQWLPT
MCMNNVMYLIPRQDGHIVCGSSMAHCGFNTLPDSTTKQNIVDACLEMVPELAQFPIVKQWAGLRPSSPTGIPYIGQVPEI
DNLWINAGHFRNGLCMGPASAQLLRQLMLKQPTDINPALYSPERLSKPVMV

Sequences:

>Translated_371_residues
MHIAIIGAGVSGLLTALELIEHGCQVSIFDQQQAGQAASWAGGGILSPMYPWRYPQAVNTLAQNGKTLYQQWNQKLQPLT
GIDFEIHDTGMLIFDESDFEIGLNYAKKHREPMQHASYLDGNPLQQVNSRINSKLEQAIYFPELSNIRNPRLLQSIMAYL
KQHPLVRFFEHRPVVKLHIQQHQIGYVLDESGIKIEADQFVIATGAWSQHWSQQLDLDIPVHPVQGQMVLFKAPAQWLPT
MCMNNVMYLIPRQDGHIVCGSSMAHCGFNTLPDSTTKQNIVDACLEMVPELAQFPIVKQWAGLRPSSPTGIPYIGQVPEI
DNLWINAGHFRNGLCMGPASAQLLRQLMLKQPTDINPALYSPERLSKPVMV
>Mature_371_residues
MHIAIIGAGVSGLLTALELIEHGCQVSIFDQQQAGQAASWAGGGILSPMYPWRYPQAVNTLAQNGKTLYQQWNQKLQPLT
GIDFEIHDTGMLIFDESDFEIGLNYAKKHREPMQHASYLDGNPLQQVNSRINSKLEQAIYFPELSNIRNPRLLQSIMAYL
KQHPLVRFFEHRPVVKLHIQQHQIGYVLDESGIKIEADQFVIATGAWSQHWSQQLDLDIPVHPVQGQMVLFKAPAQWLPT
MCMNNVMYLIPRQDGHIVCGSSMAHCGFNTLPDSTTKQNIVDACLEMVPELAQFPIVKQWAGLRPSSPTGIPYIGQVPEI
DNLWINAGHFRNGLCMGPASAQLLRQLMLKQPTDINPALYSPERLSKPVMV

Specific function: Oxidative Deamination Of D-Amino Acids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=420, Percent_Identity=24.047619047619, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR012727 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 41633; Mature: 41633

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIAIIGAGVSGLLTALELIEHGCQVSIFDQQQAGQAASWAGGGILSPMYPWRYPQAVNT
CEEEEEECCHHHHHHHHHHHHCCCEEEEECCHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
LAQNGKTLYQQWNQKLQPLTGIDFEIHDTGMLIFDESDFEIGLNYAKKHREPMQHASYLD
HHHCCHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCEECCHHHHHHCCHHHHHHCCC
GNPLQQVNSRINSKLEQAIYFPELSNIRNPRLLQSIMAYLKQHPLVRFFEHRPVVKLHIQ
CCHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHCCHHHHHHCCCEEEEEEE
QHQIGYVLDESGIKIEADQFVIATGAWSQHWSQQLDLDIPVHPVQGQMVLFKAPAQWLPT
CCCCCEEECCCCCEEECCCEEEEECCHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHH
MCMNNVMYLIPRQDGHIVCGSSMAHCGFNTLPDSTTKQNIVDACLEMVPELAQFPIVKQW
HHHCCEEEEEECCCCCEEECCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
AGLRPSSPTGIPYIGQVPEIDNLWINAGHFRNGLCMGPASAQLLRQLMLKQPTDINPALY
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEECCHHHHHHHHHHHCCCCCCCCCCC
SPERLSKPVMV
CHHHHCCCCCC
>Mature Secondary Structure
MHIAIIGAGVSGLLTALELIEHGCQVSIFDQQQAGQAASWAGGGILSPMYPWRYPQAVNT
CEEEEEECCHHHHHHHHHHHHCCCEEEEECCHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
LAQNGKTLYQQWNQKLQPLTGIDFEIHDTGMLIFDESDFEIGLNYAKKHREPMQHASYLD
HHHCCHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCEECCHHHHHHCCHHHHHHCCC
GNPLQQVNSRINSKLEQAIYFPELSNIRNPRLLQSIMAYLKQHPLVRFFEHRPVVKLHIQ
CCHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHCCHHHHHHCCCEEEEEEE
QHQIGYVLDESGIKIEADQFVIATGAWSQHWSQQLDLDIPVHPVQGQMVLFKAPAQWLPT
CCCCCEEECCCCCEEECCCEEEEECCHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHH
MCMNNVMYLIPRQDGHIVCGSSMAHCGFNTLPDSTTKQNIVDACLEMVPELAQFPIVKQW
HHHCCEEEEEECCCCCEEECCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
AGLRPSSPTGIPYIGQVPEIDNLWINAGHFRNGLCMGPASAQLLRQLMLKQPTDINPALY
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEECCHHHHHHHHHHHCCCCCCCCCCC
SPERLSKPVMV
CHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 14 {D-glutamine}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8682785; 10984043; 8097014 [H]