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The map label for this gene is sbcD [H]

Identifier: 50084125

GI number: 50084125

Start: 900942

End: 902192

Strand: Reverse

Name: sbcD [H]

Synonym: ACIAD0917

Alternate gene names: 50084125

Gene position: 902192-900942 (Counterclockwise)

Preceding gene: 50084127

Following gene: 50084124

Centisome position: 25.07

GC content: 42.13

Gene sequence:

>1251_bases
ATGGCCGTGCGTTTTTTTCATACTTCAGACTGGCACTTGGGGCAGTTTTTTTATAATCACTCCCGCCACTATGAACATGA
ACAATTTCTGGCATGGTTACTTAAGCAGATCAAGGCCAAACAGCCACATGCCCTGCTGATCGCTGGTGACATTTTTGATG
TCATTAATCCAAGTTCGCAGGCACAGAAGCAACTTTATCAGTTTCTGGCCGATGCCCATGCACTTGCTCCACACATGCAA
ACGCTGATGATTGCTGGTAATCACGATTCGGGTTATCGCATTGAACAAGTTGAGCCTTTGCTTGAAAAATACAATGCCAG
AACTGTAGGTGTCATTCGCTGGAATGAAGATAAAACACTTAATCTGGAACGCTTGATCCTGCCTGTTTATGATGAAAATA
AAAACGTGGTTGCATGGTGTATTGCGCTGCCCTTTCTACGTTCGGCCGAAATTACAGGCTTTAACGAGCATACCAACAAT
AGCCAAAGCGCGATTGCTTACTTGCATGAACAATTGATTTCAGAAGCCAAACGACGTAAAACGGATGATCAAGCGATTAT
TTTGATGTCACATGCGCATATGCAAGGCGGTGAAACCTCAGACTCCGAACGCCCGATTATTATTGGCAATGAAGAAGCTC
TTTCGACTGCGCTATTTGACGATGCAATTGATTATGTCGCACTGGGGCATTTGCATAAACCCCAGAAAGTACAGCATCCG
CACATTCGTTATAGTGGCTCGCCTATTCCACTGTCATTTAGTGAAATTAACTATAAACATCAAGTAGTCGATGTGAATAT
TGATCCTACGCAGAGCACAGATAAATTCCAGTTTGAAGCAATCGAAATTCCAAGAAGTATAAAACTGCATAAAATTCGTG
GTGAACTCAATGAGGTTATACAGCAGTTAAAATCATTGGCGCAGGGTGAAATTGAATCAATTGATTTACGCGAATATATC
GATATTGAATATCATACGCTTACACCACCATTACCCAATCTCAGACAACAATTTGAAGATGCATTGCCCCCCAAGCGCTA
TCGATTGGTTCGAATCTCACGGCAATATTTGTCACAGGACAACAACGCAGCCCAAGCAAGCAAAATTAATCTAGAACCAC
CGACTCCCGAAAAACTGTTTGAACAAATCTGGGATAAAAAAGGCTATCACGCAGATGATGACGTCAAAAAAGACTTTATG
AGCCTGGTCGCTCAGGCACAACAACAACTTGAAGATCAACACAAATACTAA

Upstream 100 bases:

>100_bases
TGAGTAAAAACCGAAGTAGCTTTTTCACTCGGTAGAATCTGAATTAGACTGTGATTTATGCTACCTTATCTTCTCATCAA
AAACAGGAAATTCATCAGCA

Downstream 100 bases:

>100_bases
AGGATTGCCGTGAAAATTTTATCAATCCGACTTAAAAATTTAGCCTCTTTGGCAGGTGAGCATTTTATTGATTTTGAAAG
TGAGCCTTTAGCCAGTGCAG

Product: ATP-dependent dsDNA exonuclease (suppression of recBC)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MAVRFFHTSDWHLGQFFYNHSRHYEHEQFLAWLLKQIKAKQPHALLIAGDIFDVINPSSQAQKQLYQFLADAHALAPHMQ
TLMIAGNHDSGYRIEQVEPLLEKYNARTVGVIRWNEDKTLNLERLILPVYDENKNVVAWCIALPFLRSAEITGFNEHTNN
SQSAIAYLHEQLISEAKRRKTDDQAIILMSHAHMQGGETSDSERPIIIGNEEALSTALFDDAIDYVALGHLHKPQKVQHP
HIRYSGSPIPLSFSEINYKHQVVDVNIDPTQSTDKFQFEAIEIPRSIKLHKIRGELNEVIQQLKSLAQGEIESIDLREYI
DIEYHTLTPPLPNLRQQFEDALPPKRYRLVRISRQYLSQDNNAAQASKINLEPPTPEKLFEQIWDKKGYHADDDVKKDFM
SLVAQAQQQLEDQHKY

Sequences:

>Translated_416_residues
MAVRFFHTSDWHLGQFFYNHSRHYEHEQFLAWLLKQIKAKQPHALLIAGDIFDVINPSSQAQKQLYQFLADAHALAPHMQ
TLMIAGNHDSGYRIEQVEPLLEKYNARTVGVIRWNEDKTLNLERLILPVYDENKNVVAWCIALPFLRSAEITGFNEHTNN
SQSAIAYLHEQLISEAKRRKTDDQAIILMSHAHMQGGETSDSERPIIIGNEEALSTALFDDAIDYVALGHLHKPQKVQHP
HIRYSGSPIPLSFSEINYKHQVVDVNIDPTQSTDKFQFEAIEIPRSIKLHKIRGELNEVIQQLKSLAQGEIESIDLREYI
DIEYHTLTPPLPNLRQQFEDALPPKRYRLVRISRQYLSQDNNAAQASKINLEPPTPEKLFEQIWDKKGYHADDDVKKDFM
SLVAQAQQQLEDQHKY
>Mature_415_residues
AVRFFHTSDWHLGQFFYNHSRHYEHEQFLAWLLKQIKAKQPHALLIAGDIFDVINPSSQAQKQLYQFLADAHALAPHMQT
LMIAGNHDSGYRIEQVEPLLEKYNARTVGVIRWNEDKTLNLERLILPVYDENKNVVAWCIALPFLRSAEITGFNEHTNNS
QSAIAYLHEQLISEAKRRKTDDQAIILMSHAHMQGGETSDSERPIIIGNEEALSTALFDDAIDYVALGHLHKPQKVQHPH
IRYSGSPIPLSFSEINYKHQVVDVNIDPTQSTDKFQFEAIEIPRSIKLHKIRGELNEVIQQLKSLAQGEIESIDLREYID
IEYHTLTPPLPNLRQQFEDALPPKRYRLVRISRQYLSQDNNAAQASKINLEPPTPEKLFEQIWDKKGYHADDDVKKDFMS
LVAQAQQQLEDQHKY

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=314, Percent_Identity=33.1210191082803, Blast_Score=145, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 48077; Mature: 47946

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVRFFHTSDWHLGQFFYNHSRHYEHEQFLAWLLKQIKAKQPHALLIAGDIFDVINPSSQ
CEEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHCCCCHH
AQKQLYQFLADAHALAPHMQTLMIAGNHDSGYRIEQVEPLLEKYNARTVGVIRWNEDKTL
HHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCC
NLERLILPVYDENKNVVAWCIALPFLRSAEITGFNEHTNNSQSAIAYLHEQLISEAKRRK
CCEEEEEEEEECCCCEEEHEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
TDDQAIILMSHAHMQGGETSDSERPIIIGNEEALSTALFDDAIDYVALGHLHKPQKVQHP
CCCCEEEEEEECCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
HIRYSGSPIPLSFSEINYKHQVVDVNIDPTQSTDKFQFEAIEIPRSIKLHKIRGELNEVI
CEEECCCCCCEEHHHCCCEEEEEEEECCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHH
QQLKSLAQGEIESIDLREYIDIEYHTLTPPLPNLRQQFEDALPPKRYRLVRISRQYLSQD
HHHHHHHCCCCCCCCHHHHCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
NNAAQASKINLEPPTPEKLFEQIWDKKGYHADDDVKKDFMSLVAQAQQQLEDQHKY
CCCCCCCEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVRFFHTSDWHLGQFFYNHSRHYEHEQFLAWLLKQIKAKQPHALLIAGDIFDVINPSSQ
EEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHCCCCHH
AQKQLYQFLADAHALAPHMQTLMIAGNHDSGYRIEQVEPLLEKYNARTVGVIRWNEDKTL
HHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCC
NLERLILPVYDENKNVVAWCIALPFLRSAEITGFNEHTNNSQSAIAYLHEQLISEAKRRK
CCEEEEEEEEECCCCEEEHEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
TDDQAIILMSHAHMQGGETSDSERPIIIGNEEALSTALFDDAIDYVALGHLHKPQKVQHP
CCCCEEEEEEECCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
HIRYSGSPIPLSFSEINYKHQVVDVNIDPTQSTDKFQFEAIEIPRSIKLHKIRGELNEVI
CEEECCCCCCEEHHHCCCEEEEEEEECCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHH
QQLKSLAQGEIESIDLREYIDIEYHTLTPPLPNLRQQFEDALPPKRYRLVRISRQYLSQD
HHHHHHHCCCCCCCCHHHHCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
NNAAQASKINLEPPTPEKLFEQIWDKKGYHADDDVKKDFMSLVAQAQQQLEDQHKY
CCCCCCCEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9256491 [H]