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The map label for this gene is pilT [H]

Identifier: 50084120

GI number: 50084120

Start: 893402

End: 894436

Strand: Reverse

Name: pilT [H]

Synonym: ACIAD0912

Alternate gene names: 50084120

Gene position: 894436-893402 (Counterclockwise)

Preceding gene: 50084123

Following gene: 50084119

Centisome position: 24.85

GC content: 44.35

Gene sequence:

>1035_bases
ATGGATATAACAGAATTACTCGCGTTTTCGGTGCAAAATCGTGCCTCTGACCTGCATTTATCCGCAGGAATTCCCCCCAT
GATCCGTGTCGATGGCGAAGTTCGTCGTATTAATCTTCCTGCAATGGATCATTCAGAAGTCCATAATCTCATTTACGATA
TTATGAATGATAAACAGCGCCGAGACTACGAAGAAAAACTGGAAACAGATTTTTCTTTTGAAGTGCCTAATCTAGCTCGT
TTTCGCGTCAATGTGTTTAATCAAAATCGTGGAGCTGGTGCTGTATTTCGGACCATTCCATCACAAGTTCTGACGCTTGA
AGAACTGGGACTGGGTAAAATTTTTCGGGATATTTGCGATTATCCGAGAGGACTCGTCCTTGTCACAGGCCCCACTGGTT
CCGGTAAATCAACCACCCTTGCAGCCATGCTCGATTATATTAACAATCATCGCTACGACCATATTTTGACTGTTGAAGAT
CCAATTGAGTTTGTACATGAGCCTAAAAAATGCCTGATCAATCAGCGTGAGGTACATCGCGATACATTAGGCTTCAATGA
AGCACTGCGCTCAGCTTTGCGTGAAGATCCAGACATTATTCTGGTTGGCGAAATGCGGGATCTGGAAACCATCCGTCTGG
CTCTTACTGCCGCAGAAACAGGGCATTTGGTTTTTGGAACACTGCATACCACATCAGCCGCGAAAACCATTGATCGTATT
ATTGATGTCTTTCCTGCCGAAGAAAAAGATATGGTTCGCACCATGTTGTCGGAATCCTTACAAGCGGTTATTTCCCAAAC
TTTATTAAAAAAAGAAGGCGGTGGACGTGTTGCAGCCCATGAAATCATGATTGGAATTCCTGCAATTCGCAATCTCATTC
GTGAAGGTAAAGTCGCTCAAATGTACTCTTCTATCCAAACAGGTGCGACACATGGCATGACGACCCTGGATCAGAGTCTC
AAACAGCTTTTGGCAAAAGGCATCATCAACTTACCAACGGCACGCGCTGTCGCAAAATCTCCAGAGTCGTTCTAA

Upstream 100 bases:

>100_bases
ATTTTTTACTTAACCTAAAGCACAAATCTTGGTAAGCTTTAATCAAACAACTCAGTATTCTACAAAATAATTAAACCTTT
TGACGATCTTCGGGGAAAAT

Downstream 100 bases:

>100_bases
GAATAACGCTACTCGATCTGAGAGGAAAACAATACGATGGACTTTAATGATTTACTCAATTTGATGGCAAAACACAAAGC
ATCTGATTTGTTTATTACAG

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MDITELLAFSVQNRASDLHLSAGIPPMIRVDGEVRRINLPAMDHSEVHNLIYDIMNDKQRRDYEEKLETDFSFEVPNLAR
FRVNVFNQNRGAGAVFRTIPSQVLTLEELGLGKIFRDICDYPRGLVLVTGPTGSGKSTTLAAMLDYINNHRYDHILTVED
PIEFVHEPKKCLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAEEKDMVRTMLSESLQAVISQTLLKKEGGGRVAAHEIMIGIPAIRNLIREGKVAQMYSSIQTGATHGMTTLDQSL
KQLLAKGIINLPTARAVAKSPESF

Sequences:

>Translated_344_residues
MDITELLAFSVQNRASDLHLSAGIPPMIRVDGEVRRINLPAMDHSEVHNLIYDIMNDKQRRDYEEKLETDFSFEVPNLAR
FRVNVFNQNRGAGAVFRTIPSQVLTLEELGLGKIFRDICDYPRGLVLVTGPTGSGKSTTLAAMLDYINNHRYDHILTVED
PIEFVHEPKKCLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAEEKDMVRTMLSESLQAVISQTLLKKEGGGRVAAHEIMIGIPAIRNLIREGKVAQMYSSIQTGATHGMTTLDQSL
KQLLAKGIINLPTARAVAKSPESF
>Mature_344_residues
MDITELLAFSVQNRASDLHLSAGIPPMIRVDGEVRRINLPAMDHSEVHNLIYDIMNDKQRRDYEEKLETDFSFEVPNLAR
FRVNVFNQNRGAGAVFRTIPSQVLTLEELGLGKIFRDICDYPRGLVLVTGPTGSGKSTTLAAMLDYINNHRYDHILTVED
PIEFVHEPKKCLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAEEKDMVRTMLSESLQAVISQTLLKKEGGGRVAAHEIMIGIPAIRNLIREGKVAQMYSSIQTGATHGMTTLDQSL
KQLLAKGIINLPTARAVAKSPESF

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=327, Percent_Identity=46.4831804281346, Blast_Score=294, Evalue=6e-81,
Organism=Escherichia coli, GI1786296, Length=280, Percent_Identity=33.9285714285714, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789723, Length=290, Percent_Identity=34.4827586206897, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38337; Mature: 38337

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDITELLAFSVQNRASDLHLSAGIPPMIRVDGEVRRINLPAMDHSEVHNLIYDIMNDKQR
CCHHHHHHHHHHHCCCCEEEECCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHCCHHH
RDYEEKLETDFSFEVPNLARFRVNVFNQNRGAGAVFRTIPSQVLTLEELGLGKIFRDICD
CCHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHC
YPRGLVLVTGPTGSGKSTTLAAMLDYINNHRYDHILTVEDPIEFVHEPKKCLINQREVHR
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCHHHHCCHHHHHH
DTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
HHCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHEEEECCCCCEEEEEEHHHHHHHHHHHH
IDVFPAEEKDMVRTMLSESLQAVISQTLLKKEGGGRVAAHEIMIGIPAIRNLIREGKVAQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCHHHHHHHHCCHHHH
MYSSIQTGATHGMTTLDQSLKQLLAKGIINLPTARAVAKSPESF
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCC
>Mature Secondary Structure
MDITELLAFSVQNRASDLHLSAGIPPMIRVDGEVRRINLPAMDHSEVHNLIYDIMNDKQR
CCHHHHHHHHHHHCCCCEEEECCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHCCHHH
RDYEEKLETDFSFEVPNLARFRVNVFNQNRGAGAVFRTIPSQVLTLEELGLGKIFRDICD
CCHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHC
YPRGLVLVTGPTGSGKSTTLAAMLDYINNHRYDHILTVEDPIEFVHEPKKCLINQREVHR
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCHHHHCCHHHHHH
DTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
HHCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHEEEECCCCCEEEEEEHHHHHHHHHHHH
IDVFPAEEKDMVRTMLSESLQAVISQTLLKKEGGGRVAAHEIMIGIPAIRNLIREGKVAQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCHHHHHHHHCCHHHH
MYSSIQTGATHGMTTLDQSLKQLLAKGIINLPTARAVAKSPESF
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]