Click here to switch to the map view.

The map label for this gene is pilU [H]

Identifier: 50084119

GI number: 50084119

Start: 892214

End: 893365

Strand: Reverse

Name: pilU [H]

Synonym: ACIAD0911

Alternate gene names: 50084119

Gene position: 893365-892214 (Counterclockwise)

Preceding gene: 50084120

Following gene: 50084117

Centisome position: 24.83

GC content: 39.67

Gene sequence:

>1152_bases
ATGGACTTTAATGATTTACTCAATTTGATGGCAAAACACAAAGCATCTGATTTGTTTATTACAGCAGGTGTAGAACCATC
TTTAAAAATTAACGGTGAAATTACCCCCGTCTCTAAAACCAAACTCGATGGAGATATGATCGCTCAGTTGATTGATTCGA
TTACAACGCAAAAGCAACGACAAGAATTCATTGAAACGCATGAATGTAATTTTGCCATCTTAAATCGTGAAAAAAACGAA
CGTTTCCGGGTGAGCGCATTTCAACAGCGTGACATGCCTGGTATGGTGATTCGTCGTATTGAGACCAAGATCCCCTCTCC
TGACGATTTACATCTACCTCCAATTTTAAAAGAACTGGCCATGAGCAAACGTGGCATTATTATTTTTTCTGGGGCAACTG
GTACAGGAAAATCCACGTCTTTGGCATCCATGATTCAACATCGTAATCAGCACTCAAAAGGTCATATTATTACTATTGAG
GATCCGATTGAATTTATACATGAGCATGCTGGATGTATTATCACTCAGCGTGAAGTCGGCATTGATACAGATTCTTTTGA
AATCGCGCTTAAAAATACGTTGCGCCAGGCGCCTGATGTGATTCTGATTGGTGAAATTCGCTCACGTGAAGTGATGGACT
ATGCTTTAGCTTTTGCAGAAACTGGCCATTTAGTATTTGCAACTTTACATGCCAATAATGCCAATCAAACCTTAGAACGG
ATTATTCACTTCTTTGATGCAGATCGACATCAGCAAATTTTTATGGATTTATCGCTCAATCTTAAAGCGGTGATTTCACA
ACAGCTTATTCCAACCCATGATGGTCAAAGTCGACGTGCTGCCGTCGAAGTGCTGATTAATACGCCTTTACTGTCAGATT
ACATTCGTCAAGGTGAAATCTATAAAATTAAGGAATTAATGGCTCGTTCTGGAGAACTTGGCATGCAAACCTTTGATCAG
GCACTTTTTGATTTATATGATCATCAGCACATCAGTTATGCAGATGCGCTCAAGCATGCAGACTCGCCTAATAATTTACG
TCTACAGATCAAGCTTTCGGAAGAAGCCAACAAACCCCTAGATAACAGAGAAAGAATGTCCGAAATGGAACGGAAAATGA
CTTTTGATGGTCAACGCCGTAATCTCAAATAA

Upstream 100 bases:

>100_bases
GGCAAAAGGCATCATCAACTTACCAACGGCACGCGCTGTCGCAAAATCTCCAGAGTCGTTCTAAGAATAACGCTACTCGA
TCTGAGAGGAAAACAATACG

Downstream 100 bases:

>100_bases
AAGAGGGCAATTTTGCCCTCTTTTAATTGTTGTATTTATGTGATTTTACTGCTTTTTACGATATGCTTCCTGACACTCAG
TCGCATCGCAATAACCATAT

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MDFNDLLNLMAKHKASDLFITAGVEPSLKINGEITPVSKTKLDGDMIAQLIDSITTQKQRQEFIETHECNFAILNREKNE
RFRVSAFQQRDMPGMVIRRIETKIPSPDDLHLPPILKELAMSKRGIIIFSGATGTGKSTSLASMIQHRNQHSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYALAFAETGHLVFATLHANNANQTLER
IIHFFDADRHQQIFMDLSLNLKAVISQQLIPTHDGQSRRAAVEVLINTPLLSDYIRQGEIYKIKELMARSGELGMQTFDQ
ALFDLYDHQHISYADALKHADSPNNLRLQIKLSEEANKPLDNRERMSEMERKMTFDGQRRNLK

Sequences:

>Translated_383_residues
MDFNDLLNLMAKHKASDLFITAGVEPSLKINGEITPVSKTKLDGDMIAQLIDSITTQKQRQEFIETHECNFAILNREKNE
RFRVSAFQQRDMPGMVIRRIETKIPSPDDLHLPPILKELAMSKRGIIIFSGATGTGKSTSLASMIQHRNQHSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYALAFAETGHLVFATLHANNANQTLER
IIHFFDADRHQQIFMDLSLNLKAVISQQLIPTHDGQSRRAAVEVLINTPLLSDYIRQGEIYKIKELMARSGELGMQTFDQ
ALFDLYDHQHISYADALKHADSPNNLRLQIKLSEEANKPLDNRERMSEMERKMTFDGQRRNLK
>Mature_383_residues
MDFNDLLNLMAKHKASDLFITAGVEPSLKINGEITPVSKTKLDGDMIAQLIDSITTQKQRQEFIETHECNFAILNREKNE
RFRVSAFQQRDMPGMVIRRIETKIPSPDDLHLPPILKELAMSKRGIIIFSGATGTGKSTSLASMIQHRNQHSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYALAFAETGHLVFATLHANNANQTLER
IIHFFDADRHQQIFMDLSLNLKAVISQQLIPTHDGQSRRAAVEVLINTPLLSDYIRQGEIYKIKELMARSGELGMQTFDQ
ALFDLYDHQHISYADALKHADSPNNLRLQIKLSEEANKPLDNRERMSEMERKMTFDGQRRNLK

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=330, Percent_Identity=36.0606060606061, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1789723, Length=156, Percent_Identity=37.8205128205128, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786296, Length=156, Percent_Identity=36.5384615384615, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 43618; Mature: 43618

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFNDLLNLMAKHKASDLFITAGVEPSLKINGEITPVSKTKLDGDMIAQLIDSITTQKQR
CCHHHHHHHHHHHCCCCEEEEECCCCCEEECCEECCCHHHHCCHHHHHHHHHHHHHHHHH
QEFIETHECNFAILNREKNERFRVSAFQQRDMPGMVIRRIETKIPSPDDLHLPPILKELA
HHHHHHCCCCEEEEECCCCCCEEHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHH
MSKRGIIIFSGATGTGKSTSLASMIQHRNQHSKGHIITIEDPIEFIHEHAGCIITQREVG
HCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCEEEECCHHHHHHHCCCEEEEECCCC
IDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYALAFAETGHLVFATLHANNANQTLER
CCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHH
IIHFFDADRHQQIFMDLSLNLKAVISQQLIPTHDGQSRRAAVEVLINTPLLSDYIRQGEI
HHHHHCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHEECCCHHHHHHHCCCH
YKIKELMARSGELGMQTFDQALFDLYDHQHISYADALKHADSPNNLRLQIKLSEEANKPL
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCC
DNRERMSEMERKMTFDGQRRNLK
CHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDFNDLLNLMAKHKASDLFITAGVEPSLKINGEITPVSKTKLDGDMIAQLIDSITTQKQR
CCHHHHHHHHHHHCCCCEEEEECCCCCEEECCEECCCHHHHCCHHHHHHHHHHHHHHHHH
QEFIETHECNFAILNREKNERFRVSAFQQRDMPGMVIRRIETKIPSPDDLHLPPILKELA
HHHHHHCCCCEEEEECCCCCCEEHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHH
MSKRGIIIFSGATGTGKSTSLASMIQHRNQHSKGHIITIEDPIEFIHEHAGCIITQREVG
HCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCEEEECCHHHHHHHCCCEEEEECCCC
IDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYALAFAETGHLVFATLHANNANQTLER
CCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHH
IIHFFDADRHQQIFMDLSLNLKAVISQQLIPTHDGQSRRAAVEVLINTPLLSDYIRQGEI
HHHHHCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHEECCCHHHHHHHCCCH
YKIKELMARSGELGMQTFDQALFDLYDHQHISYADALKHADSPNNLRLQIKLSEEANKPL
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCC
DNRERMSEMERKMTFDGQRRNLK
CHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]