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The map label for this gene is rep [H]

Identifier: 50084109

GI number: 50084109

Start: 882754

End: 884793

Strand: Direct

Name: rep [H]

Synonym: ACIAD0900

Alternate gene names: 50084109

Gene position: 882754-884793 (Clockwise)

Preceding gene: 50084108

Following gene: 50084110

Centisome position: 24.53

GC content: 41.08

Gene sequence:

>2040_bases
ATGTCTGCAGCCAGTCATTTAAATGACAAACAACAAGAAGCCATGAAATATACTCAAGGACCGTTGTTAGTGCTTGCAGG
AGCAGGTTCAGGCAAAACTTCCGTTATCACGCAAAAAATTGCATATTTAGTCAAGCAATGTCGTATTCCTGCGCATCGTA
TTACCGCGATGACCTTTACCAACAAAGCTGCACGTGAGATGAAAGAACGTGTAGCTAAGTTACTTTCGCGTGAAGATGCA
AAAGGTTTATCTGTTTCAACGTTTCATACCTTTGGTCTGAATTTATTAAGATTAGAGCTTAAACATACACCCCTGAAAAA
TAATTTTTCAATTTTGGATTCGGATGACTGCAAACGCATCCTGATGGACTTGATGCAACGTGACAATCTTTCAGGTGCAG
AAAGCAAAGAATTGATTGCCAAAGCCATGAAAAAGATTTCAGACTGGAAAAATGATCTGATTGTGCCCGAACAAGCACAT
TCAACATGTGAAACCGTTGAAGACGTTCAGTTTGCACACCTCTATCAACTCTATGAGCGTAATTTGCGTGCCTACAATGC
AGTCGATTTTGACGACCTGATTGTATTACCTACACGGCTGCTTCAGGAAAATGCTGAAGTACGTGACAAGTGGCAAAACC
GTACTCGTTATTTATTGGTGGATGAATATCAAGACACCAATACAGCACAGTATATTTTAGTTAAGCTCTTGGTTGGGGTG
ATGGGGCAGTTTACTGCGGTAGGCGATGATGACCAGTCGATTTACGCTTGGCGTGGTGCTAAACCAGAAAACATGGCGCT
ATTGAAAGAAGATTTTCCAAATTTAAAAGTCATTAAGCTTGAACAGAATTATCGTTCGACCAGTCGAATTTTGAAAGCAG
CCAACGCCGTTATTGAAAACAATCCACATATTTTTGATAAAAAGCTGTGGAGTGACAAAGGGCATGGTGAAGTGATTCGG
ATTATTACCTGTCGTAATGATGATGATGAGTCTGAACGTGTGGTCAAGGATTTGATGACTCATAAGCTGATGAATGGAAA
AAACTGGAAGGATTATGCAGTACTGTATCGCGGTAACTTTCAGGCACGAGTTTTAGAAACCCAGCTACGTCAGATGCAGA
TTCCTTATAAGCTTTCGGGTGGACAATCATTTTTTGCGCGGGCTGAAATCAAAGACGTTATGAGTTATTTGCGTTTGATT
ATTAACCCAGAAGATGATAGTGCTTTTTTGCGTATTATTAATACGCCTAAACGCGCGATTGGACCTGTGACACTGGAAAA
GCTTGGACTATTTTCTCAAGAAAATCATTTATCTTTATTGGCTGCTGCCGCTGACCAGCGTTTGAGTATGGTGATTCCGA
AAAAAGCTGCATCTCAATTACATGAATTTGCAGATTTTATTACCACATTTACCCGTGAATTATTAGATGATGACGAGCCT
GTGCCTAAGGTGCGTCAGATGATGAATGAAGCGGGCTATATTGATTATATCCGTGAGCAGGCAGCAACCCCTGCGCAAGA
AAAAACCAAGCTCGATAATATCGAGAGTTTATTTAGCAGCATCCAAAACCTGTTAAATCGTACCGAAGATGTCGATGAGA
AAAATATTGAAAGTGTGATTCGTAAGCTGGTTTTGCTCGATATGCTGGAACAACAGCAGGAAGAAGAAGATACCGATAAG
GTCAATCTTTTAACTTTGCATGCAGCTAAAGGTCTAGAGTTTCCATTTGTTTATATTATGGGGCTTGAGGAAGAGTTGTT
GCCACACAAAAACTCAATTGCAGCAGATACGATTGAAGAAGAGCGTCGCTTGATGTACGTGGGAATTACCCGTGCGCGTC
AAGGCTTGACTCTAACGCTTGCCGAGCAACGTAAAAATGGCGGACAAATGAAACAAATGACACCAAGCCGCTTTCTGGAT
GAATTGCCTCAAGATGAGCTGGAATGGCTAGGTCGTAAGAAAAAGCTGGCAGGCAATGTTGATCCGAAAGAGCAGGCTCA
GCAGTATTTAGCCAATTTAAAAGCGTTATTAAAGAAATAA

Upstream 100 bases:

>100_bases
CCTTCGGGTGGTTTTTTTATGAGTAAAATCCCTTGTTTTCGATTAGTCATCTTGCTGTTAGATGTCTATAATCATGCTCG
GATCAATCAGAGGAATACTT

Downstream 100 bases:

>100_bases
TTGATAAACCAATTAGGAACTGTAATGAAAGTCCAAGTTAAAATTTTAGATCAGCGTTTAGGTCAGGAATGGCCTTTGCC
TTCTTATGCCACTACGGGTT

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 679; Mature: 678

Protein sequence:

>679_residues
MSAASHLNDKQQEAMKYTQGPLLVLAGAGSGKTSVITQKIAYLVKQCRIPAHRITAMTFTNKAAREMKERVAKLLSREDA
KGLSVSTFHTFGLNLLRLELKHTPLKNNFSILDSDDCKRILMDLMQRDNLSGAESKELIAKAMKKISDWKNDLIVPEQAH
STCETVEDVQFAHLYQLYERNLRAYNAVDFDDLIVLPTRLLQENAEVRDKWQNRTRYLLVDEYQDTNTAQYILVKLLVGV
MGQFTAVGDDDQSIYAWRGAKPENMALLKEDFPNLKVIKLEQNYRSTSRILKAANAVIENNPHIFDKKLWSDKGHGEVIR
IITCRNDDDESERVVKDLMTHKLMNGKNWKDYAVLYRGNFQARVLETQLRQMQIPYKLSGGQSFFARAEIKDVMSYLRLI
INPEDDSAFLRIINTPKRAIGPVTLEKLGLFSQENHLSLLAAAADQRLSMVIPKKAASQLHEFADFITTFTRELLDDDEP
VPKVRQMMNEAGYIDYIREQAATPAQEKTKLDNIESLFSSIQNLLNRTEDVDEKNIESVIRKLVLLDMLEQQQEEEDTDK
VNLLTLHAAKGLEFPFVYIMGLEEELLPHKNSIAADTIEEERRLMYVGITRARQGLTLTLAEQRKNGGQMKQMTPSRFLD
ELPQDELEWLGRKKKLAGNVDPKEQAQQYLANLKALLKK

Sequences:

>Translated_679_residues
MSAASHLNDKQQEAMKYTQGPLLVLAGAGSGKTSVITQKIAYLVKQCRIPAHRITAMTFTNKAAREMKERVAKLLSREDA
KGLSVSTFHTFGLNLLRLELKHTPLKNNFSILDSDDCKRILMDLMQRDNLSGAESKELIAKAMKKISDWKNDLIVPEQAH
STCETVEDVQFAHLYQLYERNLRAYNAVDFDDLIVLPTRLLQENAEVRDKWQNRTRYLLVDEYQDTNTAQYILVKLLVGV
MGQFTAVGDDDQSIYAWRGAKPENMALLKEDFPNLKVIKLEQNYRSTSRILKAANAVIENNPHIFDKKLWSDKGHGEVIR
IITCRNDDDESERVVKDLMTHKLMNGKNWKDYAVLYRGNFQARVLETQLRQMQIPYKLSGGQSFFARAEIKDVMSYLRLI
INPEDDSAFLRIINTPKRAIGPVTLEKLGLFSQENHLSLLAAAADQRLSMVIPKKAASQLHEFADFITTFTRELLDDDEP
VPKVRQMMNEAGYIDYIREQAATPAQEKTKLDNIESLFSSIQNLLNRTEDVDEKNIESVIRKLVLLDMLEQQQEEEDTDK
VNLLTLHAAKGLEFPFVYIMGLEEELLPHKNSIAADTIEEERRLMYVGITRARQGLTLTLAEQRKNGGQMKQMTPSRFLD
ELPQDELEWLGRKKKLAGNVDPKEQAQQYLANLKALLKK
>Mature_678_residues
SAASHLNDKQQEAMKYTQGPLLVLAGAGSGKTSVITQKIAYLVKQCRIPAHRITAMTFTNKAAREMKERVAKLLSREDAK
GLSVSTFHTFGLNLLRLELKHTPLKNNFSILDSDDCKRILMDLMQRDNLSGAESKELIAKAMKKISDWKNDLIVPEQAHS
TCETVEDVQFAHLYQLYERNLRAYNAVDFDDLIVLPTRLLQENAEVRDKWQNRTRYLLVDEYQDTNTAQYILVKLLVGVM
GQFTAVGDDDQSIYAWRGAKPENMALLKEDFPNLKVIKLEQNYRSTSRILKAANAVIENNPHIFDKKLWSDKGHGEVIRI
ITCRNDDDESERVVKDLMTHKLMNGKNWKDYAVLYRGNFQARVLETQLRQMQIPYKLSGGQSFFARAEIKDVMSYLRLII
NPEDDSAFLRIINTPKRAIGPVTLEKLGLFSQENHLSLLAAAADQRLSMVIPKKAASQLHEFADFITTFTRELLDDDEPV
PKVRQMMNEAGYIDYIREQAATPAQEKTKLDNIESLFSSIQNLLNRTEDVDEKNIESVIRKLVLLDMLEQQQEEEDTDKV
NLLTLHAAKGLEFPFVYIMGLEEELLPHKNSIAADTIEEERRLMYVGITRARQGLTLTLAEQRKNGGQMKQMTPSRFLDE
LPQDELEWLGRKKKLAGNVDPKEQAQQYLANLKALLKK

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=673, Percent_Identity=49.925705794948, Blast_Score=671, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=645, Percent_Identity=40.4651162790698, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1787196, Length=353, Percent_Identity=28.8951841359773, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322369, Length=744, Percent_Identity=27.9569892473118, Blast_Score=203, Evalue=9e-53,
Organism=Saccharomyces cerevisiae, GI6324477, Length=654, Percent_Identity=23.0886850152905, Blast_Score=81, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77705; Mature: 77574

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAASHLNDKQQEAMKYTQGPLLVLAGAGSGKTSVITQKIAYLVKQCRIPAHRITAMTFT
CCCHHHCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHEEEEHHH
NKAAREMKERVAKLLSREDAKGLSVSTFHTFGLNLLRLELKHTPLKNNFSILDSDDCKRI
HHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHCCEEEEEEECCCCCCCCCCEECCHHHHHH
LMDLMQRDNLSGAESKELIAKAMKKISDWKNDLIVPEQAHSTCETVEDVQFAHLYQLYER
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NLRAYNAVDFDDLIVLPTRLLQENAEVRDKWQNRTRYLLVDEYQDTNTAQYILVKLLVGV
HHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
MGQFTAVGDDDQSIYAWRGAKPENMALLKEDFPNLKVIKLEQNYRSTSRILKAANAVIEN
HHCCCCCCCCCCCEEEECCCCCCCCHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCC
NPHIFDKKLWSDKGHGEVIRIITCRNDDDESERVVKDLMTHKLMNGKNWKDYAVLYRGNF
CCCEEHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCC
QARVLETQLRQMQIPYKLSGGQSFFARAEIKDVMSYLRLIINPEDDSAFLRIINTPKRAI
HHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHH
GPVTLEKLGLFSQENHLSLLAAAADQRLSMVIPKKAASQLHEFADFITTFTRELLDDDEP
CCHHHHHHCCCCCCCCHHHHHHHHHCHHHEECCHHHHHHHHHHHHHHHHHHHHHCCCCCC
VPKVRQMMNEAGYIDYIREQAATPAQEKTKLDNIESLFSSIQNLLNRTEDVDEKNIESVI
CHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
RKLVLLDMLEQQQEEEDTDKVNLLTLHAAKGLEFPFVYIMGLEEELLPHKNSIAADTIEE
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEECCCHHHCCCCCCCHHHHHHH
ERRLMYVGITRARQGLTLTLAEQRKNGGQMKQMTPSRFLDELPQDELEWLGRKKKLAGNV
CCEEEEEEEHHCCCCCEEEEEHHHCCCCCCCCCCHHHHHHHCCHHHHHHHCCHHHHCCCC
DPKEQAQQYLANLKALLKK
CHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAASHLNDKQQEAMKYTQGPLLVLAGAGSGKTSVITQKIAYLVKQCRIPAHRITAMTFT
CCHHHCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHEEEEHHH
NKAAREMKERVAKLLSREDAKGLSVSTFHTFGLNLLRLELKHTPLKNNFSILDSDDCKRI
HHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHCCEEEEEEECCCCCCCCCCEECCHHHHHH
LMDLMQRDNLSGAESKELIAKAMKKISDWKNDLIVPEQAHSTCETVEDVQFAHLYQLYER
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NLRAYNAVDFDDLIVLPTRLLQENAEVRDKWQNRTRYLLVDEYQDTNTAQYILVKLLVGV
HHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
MGQFTAVGDDDQSIYAWRGAKPENMALLKEDFPNLKVIKLEQNYRSTSRILKAANAVIEN
HHCCCCCCCCCCCEEEECCCCCCCCHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCC
NPHIFDKKLWSDKGHGEVIRIITCRNDDDESERVVKDLMTHKLMNGKNWKDYAVLYRGNF
CCCEEHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCC
QARVLETQLRQMQIPYKLSGGQSFFARAEIKDVMSYLRLIINPEDDSAFLRIINTPKRAI
HHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHH
GPVTLEKLGLFSQENHLSLLAAAADQRLSMVIPKKAASQLHEFADFITTFTRELLDDDEP
CCHHHHHHCCCCCCCCHHHHHHHHHCHHHEECCHHHHHHHHHHHHHHHHHHHHHCCCCCC
VPKVRQMMNEAGYIDYIREQAATPAQEKTKLDNIESLFSSIQNLLNRTEDVDEKNIESVI
CHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
RKLVLLDMLEQQQEEEDTDKVNLLTLHAAKGLEFPFVYIMGLEEELLPHKNSIAADTIEE
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEECCCHHHCCCCCCCHHHHHHH
ERRLMYVGITRARQGLTLTLAEQRKNGGQMKQMTPSRFLDELPQDELEWLGRKKKLAGNV
CCEEEEEEEHHCCCCCEEEEEHHHCCCCCCCCCCHHHHHHHCCHHHHHHHCCHHHHCCCC
DPKEQAQQYLANLKALLKK
CHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]