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The map label for this gene is thiD [H]

Identifier: 50084086

GI number: 50084086

Start: 858160

End: 858939

Strand: Reverse

Name: thiD [H]

Synonym: ACIAD0875

Alternate gene names: 50084086

Gene position: 858939-858160 (Counterclockwise)

Preceding gene: 50084089

Following gene: 50084085

Centisome position: 23.87

GC content: 44.36

Gene sequence:

>780_bases
GTGCGCCCAACCGTACTTTGTTTCTCTGGTCTCGATCCATCTGGCGGCGCTGGATTACAAGCTGATATAGAATCCATTGG
ACAAAGTGGCGCGCATGCTGCCATCGCCTGTACTGCACTCACCATTCAAAACTCTCAACAAGTTTTTGGTTTTGAAGCAA
CGTCGAAAGAATTACTGCTTGCTCAAGCCAATGCGGTAGTCGTTGATTTACCAATTAAATGTGTTAAATCAGGTATGCTC
GGTACCACTGAGAATATCGCTGCACTTGCTCAGTTCCTACGTGAACATCCAGATTATCAATATGTACTTGATCCAGTTCT
CGTTGCCAATAGCGGCGGCTCTTTAGGCGATCAGGCAACACTAGTCAAAGCCTTTGTCGAGCTCATTCCTCTGGCAACCG
TCATTACACCAAATACAGTTGAACTCCGTGCTTTAACTGGATTAGACAACATTGAAGACGCCACACAAAAACTATTTGAT
ATGGGTGCTCAGGCGATTTTGGTGAAAGGCGGCCATGAAGCCACACCAGATCATATTCATAATCAGCTCTTTATAAAGGG
TGAACTGGTTGCCGAAAGTACCTGCCCACGTCTAGAAGGTGAATATCACGGTTCGGGATGTTCATTGGCCAGTTATATTG
CTGGTCGTCTGGCACTAGGTGATGATTTAAAAATTGCAGTGCAGCATGCAGAAGTATGGTTATTTGACGTATTAAAACAA
GCAGAAATGCCTGCTCCAAATGGGCAAAAAATTCCAAAGCGGTTTAAAGTTGAAGTTTAA

Upstream 100 bases:

>100_bases
CACAAATCACACTCAAACAATGCTAGATAAAAAATAGGGTCGAGAGTTTATTCAAACTTGCCTATACTAACTCAAATTGA
ATATTTTAAGGATAATAACT

Downstream 100 bases:

>100_bases
TGAATAATAACCCACTTTTTTCGTGTGGGTTAAATTGTGATTTAATGAATCAGTCTCGATAAAAATTTTATAGAATGAAT
ACGACTCAAAAAGGCTCAAT

Product: bifunctional hydroxy-methylpyrimidine kinase/hydroxy-phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MRPTVLCFSGLDPSGGAGLQADIESIGQSGAHAAIACTALTIQNSQQVFGFEATSKELLLAQANAVVVDLPIKCVKSGML
GTTENIAALAQFLREHPDYQYVLDPVLVANSGGSLGDQATLVKAFVELIPLATVITPNTVELRALTGLDNIEDATQKLFD
MGAQAILVKGGHEATPDHIHNQLFIKGELVAESTCPRLEGEYHGSGCSLASYIAGRLALGDDLKIAVQHAEVWLFDVLKQ
AEMPAPNGQKIPKRFKVEV

Sequences:

>Translated_259_residues
MRPTVLCFSGLDPSGGAGLQADIESIGQSGAHAAIACTALTIQNSQQVFGFEATSKELLLAQANAVVVDLPIKCVKSGML
GTTENIAALAQFLREHPDYQYVLDPVLVANSGGSLGDQATLVKAFVELIPLATVITPNTVELRALTGLDNIEDATQKLFD
MGAQAILVKGGHEATPDHIHNQLFIKGELVAESTCPRLEGEYHGSGCSLASYIAGRLALGDDLKIAVQHAEVWLFDVLKQ
AEMPAPNGQKIPKRFKVEV
>Mature_259_residues
MRPTVLCFSGLDPSGGAGLQADIESIGQSGAHAAIACTALTIQNSQQVFGFEATSKELLLAQANAVVVDLPIKCVKSGML
GTTENIAALAQFLREHPDYQYVLDPVLVANSGGSLGDQATLVKAFVELIPLATVITPNTVELRALTGLDNIEDATQKLFD
MGAQAILVKGGHEATPDHIHNQLFIKGELVAESTCPRLEGEYHGSGCSLASYIAGRLALGDDLKIAVQHAEVWLFDVLKQ
AEMPAPNGQKIPKRFKVEV

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiaminase-2 family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=253, Percent_Identity=33.596837944664, Blast_Score=117, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6325378, Length=247, Percent_Identity=31.9838056680162, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324517, Length=202, Percent_Identity=33.1683168316832, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324997, Length=184, Percent_Identity=36.4130434782609, Blast_Score=93, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR016084
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR004305
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF03070 TENA_THI-4; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 27471; Mature: 27471

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPTVLCFSGLDPSGGAGLQADIESIGQSGAHAAIACTALTIQNSQQVFGFEATSKELLL
CCCCEEEEECCCCCCCCCCCHHHHHHCCCCCCEEEEEEEEEECCCCEEEEECCCCHHEEE
AQANAVVVDLPIKCVKSGMLGTTENIAALAQFLREHPDYQYVLDPVLVANSGGSLGDQAT
EECCEEEEECCHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECEEEEECCCCCCCHHHH
LVKAFVELIPLATVITPNTVELRALTGLDNIEDATQKLFDMGAQAILVKGGHEATPDHIH
HHHHHHHHHHHHHEECCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHC
NQLFIKGELVAESTCPRLEGEYHGSGCSLASYIAGRLALGDDLKIAVQHAEVWLFDVLKQ
CEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHH
AEMPAPNGQKIPKRFKVEV
HCCCCCCCCCCCCEEECCC
>Mature Secondary Structure
MRPTVLCFSGLDPSGGAGLQADIESIGQSGAHAAIACTALTIQNSQQVFGFEATSKELLL
CCCCEEEEECCCCCCCCCCCHHHHHHCCCCCCEEEEEEEEEECCCCEEEEECCCCHHEEE
AQANAVVVDLPIKCVKSGMLGTTENIAALAQFLREHPDYQYVLDPVLVANSGGSLGDQAT
EECCEEEEECCHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECEEEEECCCCCCCHHHH
LVKAFVELIPLATVITPNTVELRALTGLDNIEDATQKLFDMGAQAILVKGGHEATPDHIH
HHHHHHHHHHHHHEECCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHC
NQLFIKGELVAESTCPRLEGEYHGSGCSLASYIAGRLALGDDLKIAVQHAEVWLFDVLKQ
CEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHH
AEMPAPNGQKIPKRFKVEV
HCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]