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The map label for this gene is dfrA [H]

Identifier: 50084061

GI number: 50084061

Start: 837903

End: 838958

Strand: Reverse

Name: dfrA [H]

Synonym: ACIAD0849

Alternate gene names: 50084061

Gene position: 838958-837903 (Counterclockwise)

Preceding gene: 50084066

Following gene: 50084054

Centisome position: 23.31

GC content: 42.14

Gene sequence:

>1056_bases
ATGAATAAAAACACCCCTATTCTGGTCACTGGTGCTACAGGATATGTCGCTGGCTGGATTATTGAGCGACTGCTTAATCA
AGGCCATACGGTGCACGCGACGGTGCGTGATCCATCTAACACCGAAAAAATCAAACACCTTACCGATATTGCAAAAAAAT
CTGAAGGAAGTATTCATTTTTTTAAAGCGGATCTTCTAAGTGAAGGTGTATTTGATGAAGCGATGCAGGGTTGTGAAATT
GTGATTCATACCGCATCACCTTTTGTAATTACCAATTATAAAGATGCTGTTAAAGACATTATTGAACCCGCTGTCAATGG
TACTGCAAATGTATTGGAGTCCGTCAACCGCACAGAATCTGTTAAACGTGTAGTCGTCACCTCTAGTATTGCCGCCACTT
ACGGCGATGCGATTGAAATTAAAGCAACATCGAATAACGAATTTGATGAAAGTTGCTGGAACAATACCAGTAATGCAACA
CATCAACCTTACTCTTATTCGAAGGTGGCAGCCGAACGCAAGGCATGGGAAATGGCAGATCAACAAAACCGTTGGTCACT
GGTCTGTATCAATCCAGCATTGGTGATGGGGCCCTCTCTGACCGCTTCTAGCCAGTCTGGAAGTATTGAACTCTTACAAC
AATTTGCCAATGGTGTGACGCTATTTGGTGTACCGTCGATGTGGAATGGTATTGTTGATGTACGCGATGTGGCCGATGCT
CACGTAGCTGCAGCTTTATTACCTGATGCCTCTGGACGCTATATTATTTCAGGGGGAAGTTTAAGCCTGCTAGATATGGG
GAAAGCACTGACTCAACGCTTTGGCTATCGCTTTCCATTTCCTCACTTTACTGTACCTAAAACTGCTTTTTCTTTCGTCG
CGCCCTTTTTAGGTTATTCACGCGAATTTGTAAAATTAAATATGGGCTATCCAATTTACTTCAATGCAAAACGTAGTCAG
GAAGAACTCAACATACAATATCGTGATATCCGAGAGAGTGTTTGTGATCATTTTCAGCAATTACTTGATGATGGCGTTAT
TAAGAAATGGTTATAG

Upstream 100 bases:

>100_bases
TTAACAATTGATTGATAGAATGTCTATAAATGGTGCAGCAAATATCCTAAGATAATTTCTTAAAATTCAATCTCTTCAAA
AACATTAATCTGGCGGAAAT

Downstream 100 bases:

>100_bases
CAATGATGTATTAGCTTGAGGTCTGTTTTTCTTCAGATTAAAACTAAGAGGCAATTTATAGACCGCCTCTTAGTTTTTTT
AAATCTTATTTGATAAAGTC

Product: dehydrogenase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MNKNTPILVTGATGYVAGWIIERLLNQGHTVHATVRDPSNTEKIKHLTDIAKKSEGSIHFFKADLLSEGVFDEAMQGCEI
VIHTASPFVITNYKDAVKDIIEPAVNGTANVLESVNRTESVKRVVVTSSIAATYGDAIEIKATSNNEFDESCWNNTSNAT
HQPYSYSKVAAERKAWEMADQQNRWSLVCINPALVMGPSLTASSQSGSIELLQQFANGVTLFGVPSMWNGIVDVRDVADA
HVAAALLPDASGRYIISGGSLSLLDMGKALTQRFGYRFPFPHFTVPKTAFSFVAPFLGYSREFVKLNMGYPIYFNAKRSQ
EELNIQYRDIRESVCDHFQQLLDDGVIKKWL

Sequences:

>Translated_351_residues
MNKNTPILVTGATGYVAGWIIERLLNQGHTVHATVRDPSNTEKIKHLTDIAKKSEGSIHFFKADLLSEGVFDEAMQGCEI
VIHTASPFVITNYKDAVKDIIEPAVNGTANVLESVNRTESVKRVVVTSSIAATYGDAIEIKATSNNEFDESCWNNTSNAT
HQPYSYSKVAAERKAWEMADQQNRWSLVCINPALVMGPSLTASSQSGSIELLQQFANGVTLFGVPSMWNGIVDVRDVADA
HVAAALLPDASGRYIISGGSLSLLDMGKALTQRFGYRFPFPHFTVPKTAFSFVAPFLGYSREFVKLNMGYPIYFNAKRSQ
EELNIQYRDIRESVCDHFQQLLDDGVIKKWL
>Mature_351_residues
MNKNTPILVTGATGYVAGWIIERLLNQGHTVHATVRDPSNTEKIKHLTDIAKKSEGSIHFFKADLLSEGVFDEAMQGCEI
VIHTASPFVITNYKDAVKDIIEPAVNGTANVLESVNRTESVKRVVVTSSIAATYGDAIEIKATSNNEFDESCWNNTSNAT
HQPYSYSKVAAERKAWEMADQQNRWSLVCINPALVMGPSLTASSQSGSIELLQQFANGVTLFGVPSMWNGIVDVRDVADA
HVAAALLPDASGRYIISGGSLSLLDMGKALTQRFGYRFPFPHFTVPKTAFSFVAPFLGYSREFVKLNMGYPIYFNAKRSQ
EELNIQYRDIRESVCDHFQQLLDDGVIKKWL

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI71987463, Length=354, Percent_Identity=31.9209039548023, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6324421, Length=355, Percent_Identity=27.887323943662, Blast_Score=119, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6321281, Length=366, Percent_Identity=28.9617486338798, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320750, Length=291, Percent_Identity=31.6151202749141, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321399, Length=280, Percent_Identity=30.7142857142857, Blast_Score=103, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 38808; Mature: 38808

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNTPILVTGATGYVAGWIIERLLNQGHTVHATVRDPSNTEKIKHLTDIAKKSEGSIHF
CCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEE
FKADLLSEGVFDEAMQGCEIVIHTASPFVITNYKDAVKDIIEPAVNGTANVLESVNRTES
EEHHHHHCCHHHHHHCCCEEEEEECCCEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VKRVVVTSSIAATYGDAIEIKATSNNEFDESCWNNTSNATHQPYSYSKVAAERKAWEMAD
HHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHC
QQNRWSLVCINPALVMGPSLTASSQSGSIELLQQFANGVTLFGVPSMWNGIVDVRDVADA
CCCCEEEEEECCCEEECCCCCCCCCCCCHHHHHHHHCCEEEEECHHHHCCCCHHHHHHHH
HVAAALLPDASGRYIISGGSLSLLDMGKALTQRFGYRFPFPHFTVPKTAFSFVAPFLGYS
HHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCC
REFVKLNMGYPIYFNAKRSQEELNIQYRDIRESVCDHFQQLLDDGVIKKWL
CEEEEEECCCCEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHCC
>Mature Secondary Structure
MNKNTPILVTGATGYVAGWIIERLLNQGHTVHATVRDPSNTEKIKHLTDIAKKSEGSIHF
CCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEE
FKADLLSEGVFDEAMQGCEIVIHTASPFVITNYKDAVKDIIEPAVNGTANVLESVNRTES
EEHHHHHCCHHHHHHCCCEEEEEECCCEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VKRVVVTSSIAATYGDAIEIKATSNNEFDESCWNNTSNATHQPYSYSKVAAERKAWEMAD
HHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHC
QQNRWSLVCINPALVMGPSLTASSQSGSIELLQQFANGVTLFGVPSMWNGIVDVRDVADA
CCCCEEEEEECCCEEECCCCCCCCCCCCHHHHHHHHCCEEEEECHHHHCCCCHHHHHHHH
HVAAALLPDASGRYIISGGSLSLLDMGKALTQRFGYRFPFPHFTVPKTAFSFVAPFLGYS
HHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCC
REFVKLNMGYPIYFNAKRSQEELNIQYRDIRESVCDHFQQLLDDGVIKKWL
CEEEEEECCCCEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]