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The map label for this gene is ligA

Identifier: 50084060

GI number: 50084060

Start: 835730

End: 837760

Strand: Direct

Name: ligA

Synonym: ACIAD0848

Alternate gene names: 50084060

Gene position: 835730-837760 (Clockwise)

Preceding gene: 50084059

Following gene: 50084062

Centisome position: 23.22

GC content: 45.49

Gene sequence:

>2031_bases
ATGATGCAGCCTGCTGATATTGTTGCGCAAATGCGTCAACTGATTCAACTGATCGCAAAGCATAATCATGCGTATTATGT
GATGGATCAACCATCAATTACGGACAACGAATATGATCAGTTGTTTCATCAGCTTAAAGCACTCGAAGCAGAGTATCCTG
AGTTAACTCAAGCGGATACACCTACCAACCGTGTTGGTGGTCAGGCACTGAGCAAGTTTGAAACGGTGACCCATGCTGTG
CCCATGTTGTCCTTGGGCAATGTATTTAATCAAGAAGACTTATTTGCATTTGCACGCCGGATTGAAGAACGTTTGCCCAA
TCAGCAGATTCAGTATGATGTCGAGTTAAAACTGGATGGTCTGGCGATTTCACTGTGGTATGAAAATGGTGTACTGGTGC
GTGGTGTAACACGAGGAGATGGCGAAACAGGAGAAGATATTACCCAAAATGTCAAAACTATTCGAAATCTACCTAAACAC
TTACAACATCATTCCGTTGAAACTCCACGCTTTTTAGAGGTTCGTGGCGAAGTACTCATGCCAAAACAGGGCTTTGAGCG
ACTTAATGCTGCTAATGAGGCCAAAGGCGAGAAGACCTTTGCCAATCCGCGTAATGCGGCAGCAGGGAGTTTACGTCAGC
TTGATCCTGCGATTGCTGCTTCACGTCCATTGGCATTTTACGCTTATGGGATTGCCCAGTGCGAGCCGCATCATGGCTTG
GGAAGTATGCATGAAAGCTTACAATGGCTGACACAACTTGGCTTTGAAATTGCCGAACGTCAATTTTTATGTTCTTCGAT
TCAGGAAGTACAGCAGTGTTATGAACAGATTCAGCAAGAACGCCCTGATCTTGCGGTTGAAATTGATGGCATGGTCATCA
AGGTAGATGATTTAAAACAGCAGCAACAGCTTGGTTTCTTAAGCCGTGAACCACGTTGGGCTACGGCGTATAAGTTTCCT
GCCGAAGTTGCCATGACCACAGTAGAAAATATTGATTGGCAGGTAGGGCGAACAGGGACACTTACTCCAGTTGCTCGTTT
GCAACCTGTATTTGTAGGTGGGGTAACCGTTTCCAATGTGACATTGCACAATATTGGTGAAATTCATCGACTAGACGTGC
GTGTTGGCGATCGGGTCAGTGTTTATCGAAGCGGTGATGTGATTCCAAAGGTTGAAAAAGTCTGGCCAGAATTTCGCCCA
GAGCTAGCCGAAATTGTGCAATTACCAGAACAATGTCCTGTTTGTTCATCGCCTGTGGTGATGCCAGAAGGCGAAGCATT
GGCACGTTGTTCTGGGGGCTTATACTGTGCTGCACAGCGTATTGAAGCCATTCGTCATTTTGTATCGCGTAAGGCCTTGG
ATATTGAAGGTTTAGGTGATCGTTGGGTAGAGTCATTATTGCATCTTGATTTACTTAAGGATGTCGCCGACATTTACCAC
TTGCACGAACACCGTGAACAATTGCTCACTATTGAGAAAATGGGCGAGAAGTCTGTACAAAATCTAATGGATGCTATTGA
AGCCAGTAAAAAAACCACATTGGCGCGTTTTATTTATGCACTTGGAATACGGGGGGTAGGTGAAACGACGGCACGAATGC
TGGCCAATACCTTTCAAACTCTAGATGCCTTAAAGCAAGCAGATATAGAAGCACTCAAGAAAACCCCAGATGTCGGTGAT
ATCACCGCAGAATGGATTTATGATTTCTTTTTGGCTGAGCATAATATTGAAGTGCTTGATCGATTGCTGGCAGCAGGAAT
TCATTGGGATGCACCGCTTGCACCGACCCGACAGCCCCTTAATGGCGAAAGCTGGGTGGTCACGGGTACATTGGAAACCA
TGGGGCGTGATGAAGCAACTCAGCGCTTGCAAGCGTTAGGGGCACGAGTAAGTGGTAGTGTATCGAGTAAAACCAAATGT
GTCGTTGCGGGTGAAAAAGCGGGTAGTAAACTCGATAAAGCCGAAAAACTGCAAATTCGTGTGATGAATGAACAGGAGTT
TCTGGCATTTTTGGCACAATACTCAGCTTAA

Upstream 100 bases:

>100_bases
GATCATCTCAGGCATCAGTTTAAGTCAAAATCTTTTATACTGCTGGTAACAAGATAAGTTTAGGGCGGACGTTTCCGCCC
TTTTATATGGTGATTTTTTT

Downstream 100 bases:

>100_bases
CATGCTGCAATCAGTCCAATCTAAGCGAATTGCATAAGTGATGACTTTATCAAATAAGATTTAAAAAAACTAAGAGGCGG
TCTATAAATTGCCTCTTAGT

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 676; Mature: 676

Protein sequence:

>676_residues
MMQPADIVAQMRQLIQLIAKHNHAYYVMDQPSITDNEYDQLFHQLKALEAEYPELTQADTPTNRVGGQALSKFETVTHAV
PMLSLGNVFNQEDLFAFARRIEERLPNQQIQYDVELKLDGLAISLWYENGVLVRGVTRGDGETGEDITQNVKTIRNLPKH
LQHHSVETPRFLEVRGEVLMPKQGFERLNAANEAKGEKTFANPRNAAAGSLRQLDPAIAASRPLAFYAYGIAQCEPHHGL
GSMHESLQWLTQLGFEIAERQFLCSSIQEVQQCYEQIQQERPDLAVEIDGMVIKVDDLKQQQQLGFLSREPRWATAYKFP
AEVAMTTVENIDWQVGRTGTLTPVARLQPVFVGGVTVSNVTLHNIGEIHRLDVRVGDRVSVYRSGDVIPKVEKVWPEFRP
ELAEIVQLPEQCPVCSSPVVMPEGEALARCSGGLYCAAQRIEAIRHFVSRKALDIEGLGDRWVESLLHLDLLKDVADIYH
LHEHREQLLTIEKMGEKSVQNLMDAIEASKKTTLARFIYALGIRGVGETTARMLANTFQTLDALKQADIEALKKTPDVGD
ITAEWIYDFFLAEHNIEVLDRLLAAGIHWDAPLAPTRQPLNGESWVVTGTLETMGRDEATQRLQALGARVSGSVSSKTKC
VVAGEKAGSKLDKAEKLQIRVMNEQEFLAFLAQYSA

Sequences:

>Translated_676_residues
MMQPADIVAQMRQLIQLIAKHNHAYYVMDQPSITDNEYDQLFHQLKALEAEYPELTQADTPTNRVGGQALSKFETVTHAV
PMLSLGNVFNQEDLFAFARRIEERLPNQQIQYDVELKLDGLAISLWYENGVLVRGVTRGDGETGEDITQNVKTIRNLPKH
LQHHSVETPRFLEVRGEVLMPKQGFERLNAANEAKGEKTFANPRNAAAGSLRQLDPAIAASRPLAFYAYGIAQCEPHHGL
GSMHESLQWLTQLGFEIAERQFLCSSIQEVQQCYEQIQQERPDLAVEIDGMVIKVDDLKQQQQLGFLSREPRWATAYKFP
AEVAMTTVENIDWQVGRTGTLTPVARLQPVFVGGVTVSNVTLHNIGEIHRLDVRVGDRVSVYRSGDVIPKVEKVWPEFRP
ELAEIVQLPEQCPVCSSPVVMPEGEALARCSGGLYCAAQRIEAIRHFVSRKALDIEGLGDRWVESLLHLDLLKDVADIYH
LHEHREQLLTIEKMGEKSVQNLMDAIEASKKTTLARFIYALGIRGVGETTARMLANTFQTLDALKQADIEALKKTPDVGD
ITAEWIYDFFLAEHNIEVLDRLLAAGIHWDAPLAPTRQPLNGESWVVTGTLETMGRDEATQRLQALGARVSGSVSSKTKC
VVAGEKAGSKLDKAEKLQIRVMNEQEFLAFLAQYSA
>Mature_676_residues
MMQPADIVAQMRQLIQLIAKHNHAYYVMDQPSITDNEYDQLFHQLKALEAEYPELTQADTPTNRVGGQALSKFETVTHAV
PMLSLGNVFNQEDLFAFARRIEERLPNQQIQYDVELKLDGLAISLWYENGVLVRGVTRGDGETGEDITQNVKTIRNLPKH
LQHHSVETPRFLEVRGEVLMPKQGFERLNAANEAKGEKTFANPRNAAAGSLRQLDPAIAASRPLAFYAYGIAQCEPHHGL
GSMHESLQWLTQLGFEIAERQFLCSSIQEVQQCYEQIQQERPDLAVEIDGMVIKVDDLKQQQQLGFLSREPRWATAYKFP
AEVAMTTVENIDWQVGRTGTLTPVARLQPVFVGGVTVSNVTLHNIGEIHRLDVRVGDRVSVYRSGDVIPKVEKVWPEFRP
ELAEIVQLPEQCPVCSSPVVMPEGEALARCSGGLYCAAQRIEAIRHFVSRKALDIEGLGDRWVESLLHLDLLKDVADIYH
LHEHREQLLTIEKMGEKSVQNLMDAIEASKKTTLARFIYALGIRGVGETTARMLANTFQTLDALKQADIEALKKTPDVGD
ITAEWIYDFFLAEHNIEVLDRLLAAGIHWDAPLAPTRQPLNGESWVVTGTLETMGRDEATQRLQALGARVSGSVSSKTKC
VVAGEKAGSKLDKAEKLQIRVMNEQEFLAFLAQYSA

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=673, Percent_Identity=50.520059435364, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=525, Percent_Identity=20.7619047619048, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_ACIAD (Q6FDW0)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045570.1
- HSSP:   O87703
- ProteinModelPortal:   Q6FDW0
- STRING:   Q6FDW0
- GeneID:   2881106
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0848
- NMPDR:   fig|62977.3.peg.760
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   HNQDYID
- PhylomeDB:   Q6FDW0
- ProtClustDB:   CLSK830527
- BioCyc:   ASP62977:ACIAD0848-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75660; Mature: 75660

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 117-117 BINDING 115-115 BINDING 138-138 BINDING 177-177 BINDING 294-294 BINDING 318-318

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQPADIVAQMRQLIQLIAKHNHAYYVMDQPSITDNEYDQLFHQLKALEAEYPELTQADT
CCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
PTNRVGGQALSKFETVTHAVPMLSLGNVFNQEDLFAFARRIEERLPNQQIQYDVELKLDG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECC
LAISLWYENGVLVRGVTRGDGETGEDITQNVKTIRNLPKHLQHHSVETPRFLEVRGEVLM
EEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEC
PKQGFERLNAANEAKGEKTFANPRNAAAGSLRQLDPAIAASRPLAFYAYGIAQCEPHHGL
CHHHHHHHCCHHHCCCCCCCCCCCCHHHCHHHHCCHHHHCCCCHHEEEEHHHCCCCCCCH
GSMHESLQWLTQLGFEIAERQFLCSSIQEVQQCYEQIQQERPDLAVEIDGMVIKVDDLKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEHHHHH
QQQLGFLSREPRWATAYKFPAEVAMTTVENIDWQVGRTGTLTPVARLQPVFVGGVTVSNV
HHHCCCCCCCCCCEEEECCHHHHHHHHHHCCCEECCCCCCCCHHHHCCEEEECCEEEECE
TLHNIGEIHRLDVRVGDRVSVYRSGDVIPKVEKVWPEFRPELAEIVQLPEQCPVCSSPVV
EHHCCCCEEEEEEEECCEEEEEECCCCCCHHHHHCHHHCHHHHHHHHCHHHCCCCCCCEE
MPEGEALARCSGGLYCAAQRIEAIRHFVSRKALDIEGLGDRWVESLLHLDLLKDVADIYH
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LHEHREQLLTIEKMGEKSVQNLMDAIEASKKTTLARFIYALGIRGVGETTARMLANTFQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LDALKQADIEALKKTPDVGDITAEWIYDFFLAEHNIEVLDRLLAAGIHWDAPLAPTRQPL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCC
NGESWVVTGTLETMGRDEATQRLQALGARVSGSVSSKTKCVVAGEKAGSKLDKAEKLQIR
CCCCEEEEEEHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEEE
VMNEQEFLAFLAQYSA
EECHHHHHHHHHHHCC
>Mature Secondary Structure
MMQPADIVAQMRQLIQLIAKHNHAYYVMDQPSITDNEYDQLFHQLKALEAEYPELTQADT
CCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
PTNRVGGQALSKFETVTHAVPMLSLGNVFNQEDLFAFARRIEERLPNQQIQYDVELKLDG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECC
LAISLWYENGVLVRGVTRGDGETGEDITQNVKTIRNLPKHLQHHSVETPRFLEVRGEVLM
EEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEC
PKQGFERLNAANEAKGEKTFANPRNAAAGSLRQLDPAIAASRPLAFYAYGIAQCEPHHGL
CHHHHHHHCCHHHCCCCCCCCCCCCHHHCHHHHCCHHHHCCCCHHEEEEHHHCCCCCCCH
GSMHESLQWLTQLGFEIAERQFLCSSIQEVQQCYEQIQQERPDLAVEIDGMVIKVDDLKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEHHHHH
QQQLGFLSREPRWATAYKFPAEVAMTTVENIDWQVGRTGTLTPVARLQPVFVGGVTVSNV
HHHCCCCCCCCCCEEEECCHHHHHHHHHHCCCEECCCCCCCCHHHHCCEEEECCEEEECE
TLHNIGEIHRLDVRVGDRVSVYRSGDVIPKVEKVWPEFRPELAEIVQLPEQCPVCSSPVV
EHHCCCCEEEEEEEECCEEEEEECCCCCCHHHHHCHHHCHHHHHHHHCHHHCCCCCCCEE
MPEGEALARCSGGLYCAAQRIEAIRHFVSRKALDIEGLGDRWVESLLHLDLLKDVADIYH
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LHEHREQLLTIEKMGEKSVQNLMDAIEASKKTTLARFIYALGIRGVGETTARMLANTFQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LDALKQADIEALKKTPDVGDITAEWIYDFFLAEHNIEVLDRLLAAGIHWDAPLAPTRQPL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCC
NGESWVVTGTLETMGRDEATQRLQALGARVSGSVSSKTKCVVAGEKAGSKLDKAEKLQIR
CCCCEEEEEEHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEEE
VMNEQEFLAFLAQYSA
EECHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA