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The map label for this gene is birA [H]

Identifier: 50084054

GI number: 50084054

Start: 828626

End: 829387

Strand: Reverse

Name: birA [H]

Synonym: ACIAD0841

Alternate gene names: 50084054

Gene position: 829387-828626 (Counterclockwise)

Preceding gene: 50084061

Following gene: 50084053

Centisome position: 23.05

GC content: 43.18

Gene sequence:

>762_bases
ATGGATTTAGAGACTCGTTTACTTCGCCAGCAGCTATCAGATGCCAACCAATTACCAGAAATTCTTTTACTGAAAGAAAT
TACGACATCGACTAATGACGATGTACGAGAGCTTGCACAAAAAGGTATTTTGACGGCATTGGTTTGCAGTAACGTTCAGA
CACAAGGTCGCGGACAACATCAACGGCAATGGATTTCACCGAAAGGGAATATTTATTTAAGCACACTGATCAATACCCAA
ACAACCTTAGATGGTCGTATGGCACTCGAAGTGGCGTTAAATATTTTACAAATGCCTGCACTTGCCAACCTTAATTTACA
AGTGAAATGGCCCAACGATTTGTATAGTTTACAAGGAAAATGGGGCGGAATTCTGGTTGAACCGCTTTCTGCTACCCAAG
CCATTGTTGGTGTCGGCATAAATGTCGTGACGCCAGAACATCACGACACCGATCAGGCAATCACTTCATTAACGGATTTA
GGCTGTCATCAACGAGAACGAACCCATTTAATCGCACAGTTGTATCTCGCAATCCAGCAAGCCAAAGACTGGTTTGATCA
TGGCTGTTATCGCCTTGCTGAGCGCTTTAATCATTATGCAGCCTTCATGCATGATCATGTCCGCTTTGAGCATCATCAGG
GCAATCTTAATGGGCATTTTGAAGGAATTAATCATGAGGGTGCAGTGCTAATCCGAACGCCAGAACAATTGCATCATTTT
TATCAAGGCCGTATGCGCCGTATCGAAAACACTTCAGGATAA

Upstream 100 bases:

>100_bases
CACCCACACCAAACAGGCCTGCCGCGAAACCAGCGATTGCGCCAATGGCTAAATATATGATTAACTCCATAAATAATTTT
CTACTCTTCTCAGATTCAAC

Downstream 100 bases:

>100_bases
ATCAATATGAAGCAATTATGGCTCGATATTGGCAATACACGGCTAAAATACTGGATTACCCAAAATGAAACGGTGATTGA
ACACGCTGCCGAACTGCATT

Product: biotin--[acetyl-CoA-carboxylase] synthetase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MDLETRLLRQQLSDANQLPEILLLKEITTSTNDDVRELAQKGILTALVCSNVQTQGRGQHQRQWISPKGNIYLSTLINTQ
TTLDGRMALEVALNILQMPALANLNLQVKWPNDLYSLQGKWGGILVEPLSATQAIVGVGINVVTPEHHDTDQAITSLTDL
GCHQRERTHLIAQLYLAIQQAKDWFDHGCYRLAERFNHYAAFMHDHVRFEHHQGNLNGHFEGINHEGAVLIRTPEQLHHF
YQGRMRRIENTSG

Sequences:

>Translated_253_residues
MDLETRLLRQQLSDANQLPEILLLKEITTSTNDDVRELAQKGILTALVCSNVQTQGRGQHQRQWISPKGNIYLSTLINTQ
TTLDGRMALEVALNILQMPALANLNLQVKWPNDLYSLQGKWGGILVEPLSATQAIVGVGINVVTPEHHDTDQAITSLTDL
GCHQRERTHLIAQLYLAIQQAKDWFDHGCYRLAERFNHYAAFMHDHVRFEHHQGNLNGHFEGINHEGAVLIRTPEQLHHF
YQGRMRRIENTSG
>Mature_253_residues
MDLETRLLRQQLSDANQLPEILLLKEITTSTNDDVRELAQKGILTALVCSNVQTQGRGQHQRQWISPKGNIYLSTLINTQ
TTLDGRMALEVALNILQMPALANLNLQVKWPNDLYSLQGKWGGILVEPLSATQAIVGVGINVVTPEHHDTDQAITSLTDL
GCHQRERTHLIAQLYLAIQQAKDWFDHGCYRLAERFNHYAAFMHDHVRFEHHQGNLNGHFEGINHEGAVLIRTPEQLHHF
YQGRMRRIENTSG

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=194, Percent_Identity=29.3814432989691, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 28732; Mature: 28732

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLETRLLRQQLSDANQLPEILLLKEITTSTNDDVRELAQKGILTALVCSNVQTQGRGQH
CCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHCCCCCCCCCH
QRQWISPKGNIYLSTLINTQTTLDGRMALEVALNILQMPALANLNLQVKWPNDLYSLQGK
HHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHCCCCC
WGGILVEPLSATQAIVGVGINVVTPEHHDTDQAITSLTDLGCHQRERTHLIAQLYLAIQQ
CCCEEEECHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AKDWFDHGCYRLAERFNHYAAFMHDHVRFEHHQGNLNGHFEGINHEGAVLIRTPEQLHHF
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEECCCCCCEEEEECHHHHHHH
YQGRMRRIENTSG
HHHHHHHHCCCCC
>Mature Secondary Structure
MDLETRLLRQQLSDANQLPEILLLKEITTSTNDDVRELAQKGILTALVCSNVQTQGRGQH
CCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHCCCCCCCCCH
QRQWISPKGNIYLSTLINTQTTLDGRMALEVALNILQMPALANLNLQVKWPNDLYSLQGK
HHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHCCCCC
WGGILVEPLSATQAIVGVGINVVTPEHHDTDQAITSLTDLGCHQRERTHLIAQLYLAIQQ
CCCEEEECHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AKDWFDHGCYRLAERFNHYAAFMHDHVRFEHHQGNLNGHFEGINHEGAVLIRTPEQLHHF
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEECCCCCCEEEEECHHHHHHH
YQGRMRRIENTSG
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]