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The map label for this gene is dapE

Identifier: 50084006

GI number: 50084006

Start: 782417

End: 783550

Strand: Reverse

Name: dapE

Synonym: ACIAD0791

Alternate gene names: 50084006

Gene position: 783550-782417 (Counterclockwise)

Preceding gene: 50084007

Following gene: 50084000

Centisome position: 21.77

GC content: 44.0

Gene sequence:

>1134_bases
ATGAACCGTTCTGACACGCTCGATCTTAGTTTAAATTTAATGCGCCGCCCTTCTGTCACTCCAGAAGATCATGATTGTCA
GGCTGTTATGGCTGAACGTTTGGCAAAAGCTGGCTTTCAGATTGAAAACATGCGTTTTGATGATGTCGATAATTTATGGG
CACGTCGTGGTACTCAACAACCTGTTTTCTGCTTTGCAGGTCATACCGATGTTGTTCCAACAGGAAATTTAGACAACTGG
AACAGTGATCCGTTTGCGCCAGAAGTACGTGATGGCATTCTTTATGGTCGTGGTGCTGCCGATATGAAAACTGCACTTGC
CGCGATGGTGGTTGCTTCTGAACGTTTTGTGGAAAAACATCCGAATCATAAAGGTTCAATTGCTTTTCTCATTACTTCGG
ACGAAGAAGGCCCATCAATCAACGGTACAGTCAAAGTGATTGAAACACTGGAAGCACGTCATGAAAAAATGACGTGGTGT
CTGGTAGGCGAGCCATCAAGTACTCATCAACTCGGTGATATTATTAAAAATGGGCGTCGTGGCTCTTTAAATGCGGTACT
TACAATAAAAGGTAAGCAAGGTCATGTGGCTTATCCGCATTTGGCGCGCAATCCAATTCATCTTGCATCGGCAGCCATAC
ATGAACTATGTGAAACAGTCTGGGATCAAGGCAATGAATACTTTCCTGCAACATCTTTCCAGATTTCCAATATCCATGCA
GGTACAGGCGCGACCAATGTCGTACCAGGCACCATGGCCGTCACTTTTAATTTCCGCTATTCGACCGAAGTGACTGCTGA
GCAGCTCAAAGAACGTGTTGTTGAAGTTTTGCAACGTCACGGTCTAGATTACGATATTGTATGGACTCATTCGGGTTTGC
CTTTCTTGACGCCAGTAGGTGAATTGGTCAATGCAGCAACCCATGCGATTAAAACTGTCACTGGTGTTGATACCCAATTA
TCGACCAGTGGTGGCACATCAGATGGACGTTTTATTGCACCAACAGGTGCTCAAGTACTTGAACTGGGTGTTCTTAATGC
ATCTATTCATCAGATCAATGAGCATGTCAATGTGGCAGACCTTGAGCCATTGGCCGAAATTTATGAAAAAATTCTGGAAC
AATTATTAGCTTAA

Upstream 100 bases:

>100_bases
AAACCTTATCTTTTGATAAGGTTTTTTTGTTTGAGCATACCCATCGTGTGAAGATTGACCTATCATATCGGGTTAGATTT
TACAGACTCAATTTTTTGGT

Downstream 100 bases:

>100_bases
TTATGAAGGGACTCAAATGAGTCCCTTTTTTATTGGTTTTGGCTATGTTTAATCGCTTTATTTTGCGCTAGAAGGTTTTG
AATTTTTGTAAGTAAATCGT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MNRSDTLDLSLNLMRRPSVTPEDHDCQAVMAERLAKAGFQIENMRFDDVDNLWARRGTQQPVFCFAGHTDVVPTGNLDNW
NSDPFAPEVRDGILYGRGAADMKTALAAMVVASERFVEKHPNHKGSIAFLITSDEEGPSINGTVKVIETLEARHEKMTWC
LVGEPSSTHQLGDIIKNGRRGSLNAVLTIKGKQGHVAYPHLARNPIHLASAAIHELCETVWDQGNEYFPATSFQISNIHA
GTGATNVVPGTMAVTFNFRYSTEVTAEQLKERVVEVLQRHGLDYDIVWTHSGLPFLTPVGELVNAATHAIKTVTGVDTQL
STSGGTSDGRFIAPTGAQVLELGVLNASIHQINEHVNVADLEPLAEIYEKILEQLLA

Sequences:

>Translated_377_residues
MNRSDTLDLSLNLMRRPSVTPEDHDCQAVMAERLAKAGFQIENMRFDDVDNLWARRGTQQPVFCFAGHTDVVPTGNLDNW
NSDPFAPEVRDGILYGRGAADMKTALAAMVVASERFVEKHPNHKGSIAFLITSDEEGPSINGTVKVIETLEARHEKMTWC
LVGEPSSTHQLGDIIKNGRRGSLNAVLTIKGKQGHVAYPHLARNPIHLASAAIHELCETVWDQGNEYFPATSFQISNIHA
GTGATNVVPGTMAVTFNFRYSTEVTAEQLKERVVEVLQRHGLDYDIVWTHSGLPFLTPVGELVNAATHAIKTVTGVDTQL
STSGGTSDGRFIAPTGAQVLELGVLNASIHQINEHVNVADLEPLAEIYEKILEQLLA
>Mature_377_residues
MNRSDTLDLSLNLMRRPSVTPEDHDCQAVMAERLAKAGFQIENMRFDDVDNLWARRGTQQPVFCFAGHTDVVPTGNLDNW
NSDPFAPEVRDGILYGRGAADMKTALAAMVVASERFVEKHPNHKGSIAFLITSDEEGPSINGTVKVIETLEARHEKMTWC
LVGEPSSTHQLGDIIKNGRRGSLNAVLTIKGKQGHVAYPHLARNPIHLASAAIHELCETVWDQGNEYFPATSFQISNIHA
GTGATNVVPGTMAVTFNFRYSTEVTAEQLKERVVEVLQRHGLDYDIVWTHSGLPFLTPVGELVNAATHAIKTVTGVDTQL
STSGGTSDGRFIAPTGAQVLELGVLNASIHQINEHVNVADLEPLAEIYEKILEQLLA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Homo sapiens, GI4501901, Length=329, Percent_Identity=24.6200607902736, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1788816, Length=371, Percent_Identity=58.7601078167116, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI1790395, Length=254, Percent_Identity=26.7716535433071, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_ACIAD (Q6FE14)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045516.1
- ProteinModelPortal:   Q6FE14
- SMR:   Q6FE14
- STRING:   Q6FE14
- MEROPS:   M20.010
- GeneID:   2878256
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0791
- NMPDR:   fig|62977.3.peg.649
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- PhylomeDB:   Q6FE14
- ProtClustDB:   PRK13009
- BioCyc:   ASP62977:ACIAD0791-MONOMER
- HAMAP:   MF_01690
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 41219; Mature: 41219

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 70-70 ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSDTLDLSLNLMRRPSVTPEDHDCQAVMAERLAKAGFQIENMRFDDVDNLWARRGTQQ
CCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHCCCCC
PVFCFAGHTDVVPTGNLDNWNSDPFAPEVRDGILYGRGAADMKTALAAMVVASERFVEKH
CEEEECCCCCCCCCCCCCCCCCCCCCCHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHC
PNHKGSIAFLITSDEEGPSINGTVKVIETLEARHEKMTWCLVGEPSSTHQLGDIIKNGRR
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHCCCC
GSLNAVLTIKGKQGHVAYPHLARNPIHLASAAIHELCETVWDQGNEYFPATSFQISNIHA
CCCEEEEEEECCCCCEECCHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEC
GTGATNVVPGTMAVTFNFRYSTEVTAEQLKERVVEVLQRHGLDYDIVWTHSGLPFLTPVG
CCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
ELVNAATHAIKTVTGVDTQLSTSGGTSDGRFIAPTGAQVLELGVLNASIHQINEHVNVAD
HHHHHHHHHHHHHHCCCCEEECCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCHH
LEPLAEIYEKILEQLLA
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNRSDTLDLSLNLMRRPSVTPEDHDCQAVMAERLAKAGFQIENMRFDDVDNLWARRGTQQ
CCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHCCCCC
PVFCFAGHTDVVPTGNLDNWNSDPFAPEVRDGILYGRGAADMKTALAAMVVASERFVEKH
CEEEECCCCCCCCCCCCCCCCCCCCCCHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHC
PNHKGSIAFLITSDEEGPSINGTVKVIETLEARHEKMTWCLVGEPSSTHQLGDIIKNGRR
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHCCCC
GSLNAVLTIKGKQGHVAYPHLARNPIHLASAAIHELCETVWDQGNEYFPATSFQISNIHA
CCCEEEEEEECCCCCEECCHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEC
GTGATNVVPGTMAVTFNFRYSTEVTAEQLKERVVEVLQRHGLDYDIVWTHSGLPFLTPVG
CCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
ELVNAATHAIKTVTGVDTQLSTSGGTSDGRFIAPTGAQVLELGVLNASIHQINEHVNVAD
HHHHHHHHHHHHHHCCCCEEECCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCHH
LEPLAEIYEKILEQLLA
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA