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The map label for this gene is pilG [H]

Identifier: 50084001

GI number: 50084001

Start: 775213

End: 775596

Strand: Direct

Name: pilG [H]

Synonym: ACIAD0786

Alternate gene names: 50084001

Gene position: 775213-775596 (Clockwise)

Preceding gene: 50083996

Following gene: 50084002

Centisome position: 21.54

GC content: 35.68

Gene sequence:

>384_bases
ATGGAAGACAAATCCAAAGTGATCAAAATCATGGTAATTGATGATTCAAAAACCATTCGTCGGACAGCTGAAACACTTTT
ACAAAAAGAAGGCTATACGGTAGTCACCGCAACAGATGGTTTTGAAGCATTGTCTAAAATTGCACAAACTAATCCTGATA
TTATTTTTGTAGATATCATGATGCCACGTTTAGATGGTTATCAAACCTGTGCTCTGATTAAAAACTCACAAAACTATCAA
CATATTCCTGTGATTATGCTTTCAAGTAAGGATGGATTGTTTGATCAGGCAAAAGGACGTATTGTGGGCTCAGATGAGTA
TCTGATTAAACCTTTTAGTAAAGATGAATTGCTCACCGCAATTCGTAATCACGTCAGTGTATAA

Upstream 100 bases:

>100_bases
CGCCGATTCATTGAAAACAAGACTTTATCTATGTCAGAATGCATCTATCTTAATCATAATCTTGTTGCCAGATTTGTGGC
AAAACCATAAGGACGAGTGA

Downstream 100 bases:

>100_bases
CACGATATTGTTTTGAGGTCTTTATGGCACGTATTTTGATTGTTGATGATTCACCAACGGAAACTTATCGTTTTCGTGAC
ATACTGACCAGACATGGCTA

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 127

Protein sequence:

>127_residues
MEDKSKVIKIMVIDDSKTIRRTAETLLQKEGYTVVTATDGFEALSKIAQTNPDIIFVDIMMPRLDGYQTCALIKNSQNYQ
HIPVIMLSSKDGLFDQAKGRIVGSDEYLIKPFSKDELLTAIRNHVSV

Sequences:

>Translated_127_residues
MEDKSKVIKIMVIDDSKTIRRTAETLLQKEGYTVVTATDGFEALSKIAQTNPDIIFVDIMMPRLDGYQTCALIKNSQNYQ
HIPVIMLSSKDGLFDQAKGRIVGSDEYLIKPFSKDELLTAIRNHVSV
>Mature_127_residues
MEDKSKVIKIMVIDDSKTIRRTAETLLQKEGYTVVTATDGFEALSKIAQTNPDIIFVDIMMPRLDGYQTCALIKNSQNYQ
HIPVIMLSSKDGLFDQAKGRIVGSDEYLIKPFSKDELLTAIRNHVSV

Specific function: Is involved in pilus biosynthesis and twitching motility. May act as a single-domain response regulator whose function may be to receive a certain environmental signal(s) and then transduce that signal to the pilus assembly machinery via specific protein-

COG id: COG0784

COG function: function code T; FOG: CheY-like receiver

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=114, Percent_Identity=32.4561403508772, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI87082012, Length=118, Percent_Identity=30.5084745762712, Blast_Score=74, Evalue=4e-15,
Organism=Escherichia coli, GI1790299, Length=117, Percent_Identity=32.4786324786325, Blast_Score=67, Evalue=4e-13,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=31.1926605504587, Blast_Score=66, Evalue=8e-13,
Organism=Escherichia coli, GI1789809, Length=114, Percent_Identity=29.8245614035088, Blast_Score=66, Evalue=8e-13,
Organism=Escherichia coli, GI1788394, Length=115, Percent_Identity=26.9565217391304, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI2367329, Length=114, Percent_Identity=35.9649122807018, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI1790860, Length=114, Percent_Identity=27.1929824561404, Blast_Score=64, Evalue=2e-12,
Organism=Escherichia coli, GI1787487, Length=103, Percent_Identity=30.0970873786408, Blast_Score=64, Evalue=2e-12,
Organism=Escherichia coli, GI1790863, Length=117, Percent_Identity=28.2051282051282, Blast_Score=64, Evalue=2e-12,
Organism=Escherichia coli, GI1786911, Length=113, Percent_Identity=30.9734513274336, Blast_Score=64, Evalue=3e-12,
Organism=Escherichia coli, GI1786784, Length=118, Percent_Identity=27.9661016949153, Blast_Score=64, Evalue=5e-12,
Organism=Escherichia coli, GI1790552, Length=115, Percent_Identity=30.4347826086957, Blast_Score=62, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 14280; Mature: 14280

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDKSKVIKIMVIDDSKTIRRTAETLLQKEGYTVVTATDGFEALSKIAQTNPDIIFVDIM
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCEEEEEEE
MPRLDGYQTCALIKNSQNYQHIPVIMLSSKDGLFDQAKGRIVGSDEYLIKPFSKDELLTA
ECCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCEEECCCCEEECCCCHHHHHHH
IRNHVSV
HHHHCCC
>Mature Secondary Structure
MEDKSKVIKIMVIDDSKTIRRTAETLLQKEGYTVVTATDGFEALSKIAQTNPDIIFVDIM
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCEEEEEEE
MPRLDGYQTCALIKNSQNYQHIPVIMLSSKDGLFDQAKGRIVGSDEYLIKPFSKDELLTA
ECCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCEEECCCCEEECCCCHHHHHHH
IRNHVSV
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8104179; 10984043 [H]