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The map label for this gene is metG

Identifier: 50083984

GI number: 50083984

Start: 752585

End: 754642

Strand: Reverse

Name: metG

Synonym: ACIAD0768

Alternate gene names: 50083984

Gene position: 754642-752585 (Counterclockwise)

Preceding gene: 50083985

Following gene: 50083983

Centisome position: 20.97

GC content: 44.61

Gene sequence:

>2058_bases
GTGCGTAAAATTTTAGTCACCAACGCCCTCCCTTATGCCAATGGACCCATCCATATGGGCCACTTACTCGGTTATATCCA
AGCAGATATTTGGGTTCGTGCCATGCGTGCAATGGGCCATGATGTGACCTACGTATGTGCGGATGATGCTCATGGTACGG
CAATCATGTTACGTGCCGAAGCCAATGGAATCTCGCCAGAAAAGCAAATTGCCAATGTACAACAAGAACATATCCGCGAT
TTTGACGGATTTGGTGTACATTTCGATCATTACGACTCGACTCACAGCGCTGAAAACAAAGTACGTTCTGAAGATATTTA
CCTAAAAAACCGTGAAGCAGGCAATATTGCCGTTCGCCCGATTACCCAGCTTTTCGATCCTGAAAAACAAATGTTTTTGT
CAGATCGTTTTATTAAAGGCACTTGCCCAAAATGTAAAGCAGAAGACCAGTATGGCGATGCATGTGAAGTATGCGGCACT
ACTTACAATGCCACCGAATTACTGAATCCGCGTTCGACTTTGAGCGGTGCAACACCCGTTGAAAAATCGTCAGATCATTA
CTTCTTTAAGTTACCCAACTTTGGTGAATACTTACAAAAATGGACACGTGACGAAGGCCGTTTGCCGGTATCGATTGCCA
ACAAGCTAGATGAATGGTTTGAAGCAGGTTTAGCCGACTGGGATATTTCACGCGATGCGCCGTATTTTGGTTTTGAAATT
CCAGATGCACCCAACAAGTATTTTTACGTGTGGGTCGATGCGCCAATTGGCTATATGTCGAGCTTTGAAAACTACATCAA
AGCCAAGCGCCCAGAGCTCAACTTTGACGATTACTGGAAAAAAGATAGCCAAAACGAAGTCTATCACTTCATTGGGAAAG
ACATTGTCTACTTCCATGCGCTGTTCTGGCCAGCGATGCTTGAAGGTGCCAACTACCGTACACCAAGTGGTTTATTCGTT
AATGGCTTCCTGACGGTCAATGGTCAGAAAATGTCGAAATCACGCGGTACATTTATTAAAGCAGAAACCTATTTACAGCA
CCTGAATCCAGAATACCTGCGCTACTACTTTGCATCGAAACTTTCGGACAAAGTGGAAGATTCAGATTTGAATCTGGATG
ATTTTGTACAAAAAGTAAATTCTGATTTGGTCGGTAAAGTGGTCAACATTGCCAGCCGCTGTGCCAAATTTATCAATAGC
AGCTTTAACAATACCCTCTCTGCAAACTGTGCAGAACCAGAACTGGTACAAAGCTTTATCGATGCAGGCGACTCTATTGC
ACAAGCCTACGAGGCGCGTGAGTTTAGCGCTGCAATTCGTGAAATTATGGCACTGGCCGACAAAGCCAACCAATATATCG
ATGAGAAAAAACCGTGGGCGCTTGCGAAGCAAGAGGGTCAAGAACAACAAGTTCATAACGTTTGCTCAGTCGGGATTAAC
TTATTCCGTCAGCTTGCGATTTACCTGTCTCCTGTGCTGCCAACACTTGCTGCTCAAGTTCAGGCTTTCTTACAACTTGA
AAGCTTTGATTTTGCATCACGTCAGCAAATCTTGCTTGCACACGAAATTGCACAGTTCCAACCACTCATGCAACGTGTAG
ATCCGAAAGCTGTGGCTGCGATGGTCGATGCATCTAAAGATTCGTTGGCTGCTACAGCGGAAGCACCAAAAGCGGAAAAG
AAAAAAGAGAAGAAAGTCGAGAAGAAACCTGAACCAAAAGTAGGTGAAGCTGAAATTATTGGTATTGAAGACTTTATAAA
AGTTGACCTTCGTGTAGCGCTTGTGCAAGAGGCTGCGACAGTTGAAGGCTCAGACAAACTGCTTCAATTGACTTTAGATG
TCGGCGAAGCTGAACCGCGCAATGTCTTTAGCGGGATTCGTGAGTTTTATCAGCCTGAAGATTTAAAAGGCAAACTGGTG
GTGATGGTAGCGAACCTTGCACCACGTAAAATGCGTTTTGGTATTTCTAACGGTATGGTTTTAGCTGCGGGTAATGGCGA
CGGTGTTTGGGTAATTTCACCAGACAGCGGTGCAAAACCGGGCGATAAGGTGTCTTAA

Upstream 100 bases:

>100_bases
TTAATTTTCCCTTCTCTGTCAAGCCATGCCTTGCTTTGTTTTCTCGGGTTCATCAGTTAGAATCATCGGTCTTATGAATA
CGAATGAGAGAATCCAATCC

Downstream 100 bases:

>100_bases
TTTTTTAGACAGCTTCAAAAAGCCCTGCTATATTTTAGTGGGGCTTTCATTAAGTTATAAATTTAAATATGAACAATTTT
ATAAAATATTCATGTCTTTA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 685; Mature: 685

Protein sequence:

>685_residues
MRKILVTNALPYANGPIHMGHLLGYIQADIWVRAMRAMGHDVTYVCADDAHGTAIMLRAEANGISPEKQIANVQQEHIRD
FDGFGVHFDHYDSTHSAENKVRSEDIYLKNREAGNIAVRPITQLFDPEKQMFLSDRFIKGTCPKCKAEDQYGDACEVCGT
TYNATELLNPRSTLSGATPVEKSSDHYFFKLPNFGEYLQKWTRDEGRLPVSIANKLDEWFEAGLADWDISRDAPYFGFEI
PDAPNKYFYVWVDAPIGYMSSFENYIKAKRPELNFDDYWKKDSQNEVYHFIGKDIVYFHALFWPAMLEGANYRTPSGLFV
NGFLTVNGQKMSKSRGTFIKAETYLQHLNPEYLRYYFASKLSDKVEDSDLNLDDFVQKVNSDLVGKVVNIASRCAKFINS
SFNNTLSANCAEPELVQSFIDAGDSIAQAYEAREFSAAIREIMALADKANQYIDEKKPWALAKQEGQEQQVHNVCSVGIN
LFRQLAIYLSPVLPTLAAQVQAFLQLESFDFASRQQILLAHEIAQFQPLMQRVDPKAVAAMVDASKDSLAATAEAPKAEK
KKEKKVEKKPEPKVGEAEIIGIEDFIKVDLRVALVQEAATVEGSDKLLQLTLDVGEAEPRNVFSGIREFYQPEDLKGKLV
VMVANLAPRKMRFGISNGMVLAAGNGDGVWVISPDSGAKPGDKVS

Sequences:

>Translated_685_residues
MRKILVTNALPYANGPIHMGHLLGYIQADIWVRAMRAMGHDVTYVCADDAHGTAIMLRAEANGISPEKQIANVQQEHIRD
FDGFGVHFDHYDSTHSAENKVRSEDIYLKNREAGNIAVRPITQLFDPEKQMFLSDRFIKGTCPKCKAEDQYGDACEVCGT
TYNATELLNPRSTLSGATPVEKSSDHYFFKLPNFGEYLQKWTRDEGRLPVSIANKLDEWFEAGLADWDISRDAPYFGFEI
PDAPNKYFYVWVDAPIGYMSSFENYIKAKRPELNFDDYWKKDSQNEVYHFIGKDIVYFHALFWPAMLEGANYRTPSGLFV
NGFLTVNGQKMSKSRGTFIKAETYLQHLNPEYLRYYFASKLSDKVEDSDLNLDDFVQKVNSDLVGKVVNIASRCAKFINS
SFNNTLSANCAEPELVQSFIDAGDSIAQAYEAREFSAAIREIMALADKANQYIDEKKPWALAKQEGQEQQVHNVCSVGIN
LFRQLAIYLSPVLPTLAAQVQAFLQLESFDFASRQQILLAHEIAQFQPLMQRVDPKAVAAMVDASKDSLAATAEAPKAEK
KKEKKVEKKPEPKVGEAEIIGIEDFIKVDLRVALVQEAATVEGSDKLLQLTLDVGEAEPRNVFSGIREFYQPEDLKGKLV
VMVANLAPRKMRFGISNGMVLAAGNGDGVWVISPDSGAKPGDKVS
>Mature_685_residues
MRKILVTNALPYANGPIHMGHLLGYIQADIWVRAMRAMGHDVTYVCADDAHGTAIMLRAEANGISPEKQIANVQQEHIRD
FDGFGVHFDHYDSTHSAENKVRSEDIYLKNREAGNIAVRPITQLFDPEKQMFLSDRFIKGTCPKCKAEDQYGDACEVCGT
TYNATELLNPRSTLSGATPVEKSSDHYFFKLPNFGEYLQKWTRDEGRLPVSIANKLDEWFEAGLADWDISRDAPYFGFEI
PDAPNKYFYVWVDAPIGYMSSFENYIKAKRPELNFDDYWKKDSQNEVYHFIGKDIVYFHALFWPAMLEGANYRTPSGLFV
NGFLTVNGQKMSKSRGTFIKAETYLQHLNPEYLRYYFASKLSDKVEDSDLNLDDFVQKVNSDLVGKVVNIASRCAKFINS
SFNNTLSANCAEPELVQSFIDAGDSIAQAYEAREFSAAIREIMALADKANQYIDEKKPWALAKQEGQEQQVHNVCSVGIN
LFRQLAIYLSPVLPTLAAQVQAFLQLESFDFASRQQILLAHEIAQFQPLMQRVDPKAVAAMVDASKDSLAATAEAPKAEK
KKEKKVEKKPEPKVGEAEIIGIEDFIKVDLRVALVQEAATVEGSDKLLQLTLDVGEAEPRNVFSGIREFYQPEDLKGKLV
VMVANLAPRKMRFGISNGMVLAAGNGDGVWVISPDSGAKPGDKVS

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=579, Percent_Identity=29.1882556131261, Blast_Score=247, Evalue=3e-65,
Organism=Homo sapiens, GI24308436, Length=532, Percent_Identity=25.187969924812, Blast_Score=148, Evalue=2e-35,
Organism=Homo sapiens, GI215490011, Length=102, Percent_Identity=32.3529411764706, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI215490009, Length=102, Percent_Identity=32.3529411764706, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI45006986, Length=102, Percent_Identity=32.3529411764706, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI4507947, Length=140, Percent_Identity=35, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788432, Length=685, Percent_Identity=58.5401459854015, Blast_Score=837, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=618, Percent_Identity=28.3171521035599, Blast_Score=272, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI212645096, Length=515, Percent_Identity=22.7184466019417, Blast_Score=118, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI71992781, Length=341, Percent_Identity=25.5131964809384, Blast_Score=108, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6321704, Length=556, Percent_Identity=28.4172661870504, Blast_Score=244, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6321610, Length=544, Percent_Identity=23.8970588235294, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321333, Length=98, Percent_Identity=38.7755102040816, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24655311, Length=557, Percent_Identity=28.5457809694794, Blast_Score=241, Evalue=1e-63,
Organism=Drosophila melanogaster, GI21358483, Length=516, Percent_Identity=25.5813953488372, Blast_Score=154, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24651924, Length=101, Percent_Identity=41.5841584158416, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21357223, Length=88, Percent_Identity=40.9090909090909, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_ACIAD (Q6FE36)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045494.1
- ProteinModelPortal:   Q6FE36
- STRING:   Q6FE36
- GeneID:   2879488
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0768
- NMPDR:   fig|62977.3.peg.573
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   CEAVPES
- PhylomeDB:   Q6FE36
- ProtClustDB:   PRK00133
- BioCyc:   ASP62977:ACIAD0768-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 76746; Mature: 76746

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 333-333

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKILVTNALPYANGPIHMGHLLGYIQADIWVRAMRAMGHDVTYVCADDAHGTAIMLRAE
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEC
ANGISPEKQIANVQQEHIRDFDGFGVHFDHYDSTHSAENKVRSEDIYLKNREAGNIAVRP
CCCCCHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCHHHHCCCCCEEEECCCCCCEEEEE
ITQLFDPEKQMFLSDRFIKGTCPKCKAEDQYGDACEVCGTTYNATELLNPRSTLSGATPV
HHHHHCCHHHHHHHCCHHCCCCCCCCCCCCCCCHHHHCCCCCCHHHHCCCHHHCCCCCCC
EKSSDHYFFKLPNFGEYLQKWTRDEGRLPVSIANKLDEWFEAGLADWDISRDAPYFGFEI
CCCCCCEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEC
PDAPNKYFYVWVDAPIGYMSSFENYIKAKRPELNFDDYWKKDSQNEVYHFIGKDIVYFHA
CCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHH
LFWPAMLEGANYRTPSGLFVNGFLTVNGQKMSKSRGTFIKAETYLQHLNPEYLRYYFASK
HHHHHHHCCCCCCCCCCEEEEEEEEECCCHHHCCCCCEEEHHHHHHHCCHHHHHHHHHHH
LSDKVEDSDLNLDDFVQKVNSDLVGKVVNIASRCAKFINSSFNNTLSANCAEPELVQSFI
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
DAGDSIAQAYEAREFSAAIREIMALADKANQYIDEKKPWALAKQEGQEQQVHNVCSVGIN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHH
LFRQLAIYLSPVLPTLAAQVQAFLQLESFDFASRQQILLAHEIAQFQPLMQRVDPKAVAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
MVDASKDSLAATAEAPKAEKKKEKKVEKKPEPKVGEAEIIGIEDFIKVDLRVALVQEAAT
HHHCCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHC
VEGSDKLLQLTLDVGEAEPRNVFSGIREFYQPEDLKGKLVVMVANLAPRKMRFGISNGMV
CCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHEEECCCCEE
LAAGNGDGVWVISPDSGAKPGDKVS
EEECCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MRKILVTNALPYANGPIHMGHLLGYIQADIWVRAMRAMGHDVTYVCADDAHGTAIMLRAE
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEC
ANGISPEKQIANVQQEHIRDFDGFGVHFDHYDSTHSAENKVRSEDIYLKNREAGNIAVRP
CCCCCHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCHHHHCCCCCEEEECCCCCCEEEEE
ITQLFDPEKQMFLSDRFIKGTCPKCKAEDQYGDACEVCGTTYNATELLNPRSTLSGATPV
HHHHHCCHHHHHHHCCHHCCCCCCCCCCCCCCCHHHHCCCCCCHHHHCCCHHHCCCCCCC
EKSSDHYFFKLPNFGEYLQKWTRDEGRLPVSIANKLDEWFEAGLADWDISRDAPYFGFEI
CCCCCCEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEC
PDAPNKYFYVWVDAPIGYMSSFENYIKAKRPELNFDDYWKKDSQNEVYHFIGKDIVYFHA
CCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHH
LFWPAMLEGANYRTPSGLFVNGFLTVNGQKMSKSRGTFIKAETYLQHLNPEYLRYYFASK
HHHHHHHCCCCCCCCCCEEEEEEEEECCCHHHCCCCCEEEHHHHHHHCCHHHHHHHHHHH
LSDKVEDSDLNLDDFVQKVNSDLVGKVVNIASRCAKFINSSFNNTLSANCAEPELVQSFI
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
DAGDSIAQAYEAREFSAAIREIMALADKANQYIDEKKPWALAKQEGQEQQVHNVCSVGIN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHH
LFRQLAIYLSPVLPTLAAQVQAFLQLESFDFASRQQILLAHEIAQFQPLMQRVDPKAVAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
MVDASKDSLAATAEAPKAEKKKEKKVEKKPEPKVGEAEIIGIEDFIKVDLRVALVQEAAT
HHHCCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHC
VEGSDKLLQLTLDVGEAEPRNVFSGIREFYQPEDLKGKLVVMVANLAPRKMRFGISNGMV
CCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHEEECCCCEE
LAAGNGDGVWVISPDSGAKPGDKVS
EEECCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA