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The map label for this gene is mscL

Identifier: 50083927

GI number: 50083927

Start: 692415

End: 692855

Strand: Direct

Name: mscL

Synonym: ACIAD0704

Alternate gene names: 50083927

Gene position: 692415-692855 (Clockwise)

Preceding gene: 50083926

Following gene: 50083928

Centisome position: 19.24

GC content: 38.1

Gene sequence:

>441_bases
ATGAGTATTATTCAAGAATTTAGAGAATTTGCCGTTAAAGGCAATATGATTGATTTGGCTGTGGGTGTCATCATTGGTGG
TGCTTTTGGTAAAATTGTCGATTCATTGGTGAAAGATATTATCATGCCATTGATTACTGTCATTACGGGTGGTGGTGTCG
ATTTTACCCAGAAATTTGTTGTATTGGGCAACAACCCTGATAATCTACAATCATTGGATGCATTACAAAAAGCGGGTGTG
AATGTACTCACTTATGGTAATTTCTTAACCATTTTAATTAACTTCATTATTTTGGCATGGGTTGTATTCTTAATGGTGAA
ACTCATTAACCGTATGCGTCGTAAGCAGGAAGAAGCACCTGCAGCACCAGCACCAACCCCGGAAGATATCGCACTTCTGC
GTGAAATTCGTGATGAATTAAAAAATCGTCCTCAGGTTTAA

Upstream 100 bases:

>100_bases
AAAAAACATTACATTGAATTTCATATATCAATAAAAGTTACATTTACTAAAAAATGTAATTAGAAACAAAGTGGGCAAGT
TGCCTAGCTGGAGAATTTAG

Downstream 100 bases:

>100_bases
CTGATTCATCATTAAGCGGTTGCTTGTGAAAACGGTACGCGGTGCGTACCGTTTTTTTATATGAATCGTTTGATTCTGGC
ATGGATTTTTCTTAAAAATT

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 146; Mature: 145

Protein sequence:

>146_residues
MSIIQEFREFAVKGNMIDLAVGVIIGGAFGKIVDSLVKDIIMPLITVITGGGVDFTQKFVVLGNNPDNLQSLDALQKAGV
NVLTYGNFLTILINFIILAWVVFLMVKLINRMRRKQEEAPAAPAPTPEDIALLREIRDELKNRPQV

Sequences:

>Translated_146_residues
MSIIQEFREFAVKGNMIDLAVGVIIGGAFGKIVDSLVKDIIMPLITVITGGGVDFTQKFVVLGNNPDNLQSLDALQKAGV
NVLTYGNFLTILINFIILAWVVFLMVKLINRMRRKQEEAPAAPAPTPEDIALLREIRDELKNRPQV
>Mature_145_residues
SIIQEFREFAVKGNMIDLAVGVIIGGAFGKIVDSLVKDIIMPLITVITGGGVDFTQKFVVLGNNPDNLQSLDALQKAGVN
VLTYGNFLTILINFIILAWVVFLMVKLINRMRRKQEEAPAAPAPTPEDIALLREIRDELKNRPQV

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=143, Percent_Identity=51.0489510489511, Blast_Score=133, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_ACIAD (Q6FE93)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045437.1
- ProteinModelPortal:   Q6FE93
- SMR:   Q6FE93
- STRING:   Q6FE93
- GeneID:   2879147
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0704
- NMPDR:   fig|62977.3.peg.451
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   IKATRCP
- PhylomeDB:   Q6FE93
- ProtClustDB:   PRK13952
- BioCyc:   ASP62977:ACIAD0704-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 16118; Mature: 15987

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x167ceb3c)-; HASH(0x1553d718)-; HASH(0x16611588)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIQEFREFAVKGNMIDLAVGVIIGGAFGKIVDSLVKDIIMPLITVITGGGVDFTQKFV
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEE
VLGNNPDNLQSLDALQKAGVNVLTYGNFLTILINFIILAWVVFLMVKLINRMRRKQEEAP
EECCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AAPAPTPEDIALLREIRDELKNRPQV
CCCCCCHHHHHHHHHHHHHHHCCCCH
>Mature Secondary Structure 
SIIQEFREFAVKGNMIDLAVGVIIGGAFGKIVDSLVKDIIMPLITVITGGGVDFTQKFV
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEE
VLGNNPDNLQSLDALQKAGVNVLTYGNFLTILINFIILAWVVFLMVKLINRMRRKQEEAP
EECCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AAPAPTPEDIALLREIRDELKNRPQV
CCCCCCHHHHHHHHHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA