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The map label for this gene is ogt [H]

Identifier: 50083918

GI number: 50083918

Start: 681122

End: 681625

Strand: Direct

Name: ogt [H]

Synonym: ACIAD0692

Alternate gene names: 50083918

Gene position: 681122-681625 (Clockwise)

Preceding gene: 50083917

Following gene: 50083922

Centisome position: 18.93

GC content: 40.08

Gene sequence:

>504_bases
TTGTGGAATATCATGGAACAATTATTTTATCGTTATATCGATTCTCCTGTTGGAAAACTCAAGCTAGTTGCCTCTGATAC
AGCTTTAGTGGCGATATTATGGGAAAATGAAAATCCTAAACGTATTCAACTTGCGAATTTGACTCAAGCTTTCCAGCATC
CTGTGTTATTACAGACTAAGTTACAGTTAGAAGAATATTTCTTAGGCAAACGTCAGTATTTTGAAATATCACTCGATCTT
CAAGGTACGCCCTTTCAAAGACAAGTATGGACAGCTTTGCAAACTATTCCATATGGAAAAACCTGTAGCTACAAGCAAAT
TGCCCAAATGGTGGGCCGTGAAAAAGCAATGCGTGCCGTAGGGGCTGCCAATGGAAAGAACCCTATTTCAATCATTGTGC
CATGTCATCGCGTGATTGGTGCAGCTGGCACATTGGTAGGCTTCGCGGGGGGAATAGATAAAAAACAAAAATTGCTCGCA
TTAGAATCCAGCTTTACCATTTAA

Upstream 100 bases:

>100_bases
TTTGTTCTTGCATGAGCCAATGGGGGCGATGCGGATGCTGGCTGCGGTCCTGATTGTATCGGGTCTGGTCTTGATGAAAC
TTTCTGCTCACTCATAATCA

Downstream 100 bases:

>100_bases
GCTAAAACTGGGTAGGGCTTTATTAAAATCAAAGTATTTTGAAAGTACGGTAAATATTTATGGTTCAAGATCTAATTGAC
AATTGACCATCCATTTAATA

Product: methylated-DNA--protein-cysteine methyltransferase (6-O-methylguanine-DNA methyltransferase) (O-6-methylguanine-DNA-alkyltransferase)

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MWNIMEQLFYRYIDSPVGKLKLVASDTALVAILWENENPKRIQLANLTQAFQHPVLLQTKLQLEEYFLGKRQYFEISLDL
QGTPFQRQVWTALQTIPYGKTCSYKQIAQMVGREKAMRAVGAANGKNPISIIVPCHRVIGAAGTLVGFAGGIDKKQKLLA
LESSFTI

Sequences:

>Translated_167_residues
MWNIMEQLFYRYIDSPVGKLKLVASDTALVAILWENENPKRIQLANLTQAFQHPVLLQTKLQLEEYFLGKRQYFEISLDL
QGTPFQRQVWTALQTIPYGKTCSYKQIAQMVGREKAMRAVGAANGKNPISIIVPCHRVIGAAGTLVGFAGGIDKKQKLLA
LESSFTI
>Mature_167_residues
MWNIMEQLFYRYIDSPVGKLKLVASDTALVAILWENENPKRIQLANLTQAFQHPVLLQTKLQLEEYFLGKRQYFEISLDL
QGTPFQRQVWTALQTIPYGKTCSYKQIAQMVGREKAMRAVGAANGKNPISIIVPCHRVIGAAGTLVGFAGGIDKKQKLLA
LESSFTI

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=162, Percent_Identity=32.0987654320988, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1787596, Length=169, Percent_Identity=41.4201183431953, Blast_Score=123, Evalue=6e-30,
Organism=Escherichia coli, GI1788542, Length=167, Percent_Identity=37.125748502994, Blast_Score=105, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI115533068, Length=82, Percent_Identity=48.780487804878, Blast_Score=83, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI115533070, Length=82, Percent_Identity=48.780487804878, Blast_Score=83, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6320001, Length=88, Percent_Identity=37.5, Blast_Score=76, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17137554, Length=89, Percent_Identity=43.8202247191011, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18757; Mature: 18757

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWNIMEQLFYRYIDSPVGKLKLVASDTALVAILWENENPKRIQLANLTQAFQHPVLLQTK
CCHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEEECCCCCEEEEHHHHHHHCCCEEEHHH
LQLEEYFLGKRQYFEISLDLQGTPFQRQVWTALQTIPYGKTCSYKQIAQMVGREKAMRAV
HHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
GAANGKNPISIIVPCHRVIGAAGTLVGFAGGIDKKQKLLALESSFTI
HCCCCCCCEEEEEEHHHHHCCHHHHHHHCCCCCCHHHHEEHHCCCCC
>Mature Secondary Structure
MWNIMEQLFYRYIDSPVGKLKLVASDTALVAILWENENPKRIQLANLTQAFQHPVLLQTK
CCHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEEECCCCCEEEEHHHHHHHCCCEEEHHH
LQLEEYFLGKRQYFEISLDLQGTPFQRQVWTALQTIPYGKTCSYKQIAQMVGREKAMRAV
HHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
GAANGKNPISIIVPCHRVIGAAGTLVGFAGGIDKKQKLLALESSFTI
HCCCCCCCEEEEEEHHHHHCCHHHHHHHCCCCCCHHHHEEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA