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The map label for this gene is murA [H]

Identifier: 50083890

GI number: 50083890

Start: 654040

End: 655296

Strand: Direct

Name: murA [H]

Synonym: ACIAD0660

Alternate gene names: 50083890

Gene position: 654040-655296 (Clockwise)

Preceding gene: 50083889

Following gene: 50083891

Centisome position: 18.17

GC content: 42.16

Gene sequence:

>1257_bases
ATGGACAAATTTTTAATTACGGGTGGTGTAAAACTCGAGGGTGAAGTTCGCATTTCTGGCGCCAAAAATGCGGCATTACC
TTTACTTGCGGCTATGATTTTGGCTGATTCTCCAATTACGCTGAATAACGTACCTAATCTTAAAGATGTCAATACACTGG
TCAAGCTGATTGCCGGGTTAGGTATTACAATGGATTATGAGGGTGAAACTGTTAAGGCAGACACCTCTACATTATCAAAT
CAGTTTGCACCTTATGAATTAGTAAAAACGATGCGTGCATCGATTTTAGTGCTCGGGCCATTATTGGCACGTTATGGCAA
TGCACAGGTTTCTTTACCAGGTGGGTGTGCGATTGGTTCTCGTCCTGTAGATCAGCATCTTAAAGCACTTGAGGCATTAG
GTGCTCATATTGAAGTTGAAAATGGTTATGTACATGCCACTGTGGATGGCCGTTTAAAAGGCGCAGATATCACCTTTGAT
ATGGTAACCGTCGGTGGAACAGAAAATATTCTCATGGCTGCGGTATTGGCAGAGGGTACAACCACAATTCGTAATGCTGC
ACGCGAACCTGAAATTACGGATTTGGCACAAATGTTGATTGAAATGGGCGCCAAGATTGAAGGATTAGATACAGATACTT
TAGTAGTTACAGGTGTTGAAAGTTTACATGGCTGTGAATATTCAGTTGTTGCTGATCGTATTGAGACAGGTTCTTATCTT
GCCGCTGCTGCGATTACAGGTGGGCGTGTTAAGACCACACATACGGATCCATCATTACTAGAATCAGTTCTTGATAAGTT
TGAAGAAATGGGTGCAGAAGTCACGCGTGGTGAAGACTGGATCGAATTGGATATGATGGGCAAACGTCCTAAAGCTGTAA
GTTTTAGGACATTACCGCATCCAGAATTTCCAACGGATATGCAGGCACAGTTAATGGCGGTTAATGTGATTGGTAGAGGT
TTTGCGACGATTTCAGAAACTATTTTTGAGAACCGTTTTATGCATGTCCCTGAACTGTCACGTATGGGTGCCAATATTCA
GGTCGAAGGTAATGATGCTATTGTAACGGGTGTTGAAAAATTGCAAGCAGCTCCTGTGATGGCGACTGATTTGCGTGCTT
CTTTTTCCTTGGTACTCGCAGCATTGGTTGCCGAAGGAGATACTTTGATTGACCGTATTTATCACATTGATCGTGGTTAT
GAAAATATTGAAGCTAAATTACAAAGTTTAGGCGCTAAGATTAAGCGAGTAAGTTGA

Upstream 100 bases:

>100_bases
AAATTCTTATATTGCCAGTGGTGAAGTCCATGCGGTTACTATCCGTGCAATGACTAAAGAAGAATGGCGTAAAGCAAGTC
TATTCGGAGCCTAAACTTAC

Downstream 100 bases:

>100_bases
TGAATGATATGAGAAACGATGATCCAAACTTTAACGTAATGGGGAATTTTGATCATGGTTTAACATTGGCGCTTAGTAAG
GGGCGTATTTTAAAAGAGAC

Product: UDP-N-acetylglucosamine 1-carboxyvinyltransferase

Products: NA

Alternate protein names: Enoylpyruvate transferase; UDP-N-acetylglucosamine enolpyruvyl transferase; EPT [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MDKFLITGGVKLEGEVRISGAKNAALPLLAAMILADSPITLNNVPNLKDVNTLVKLIAGLGITMDYEGETVKADTSTLSN
QFAPYELVKTMRASILVLGPLLARYGNAQVSLPGGCAIGSRPVDQHLKALEALGAHIEVENGYVHATVDGRLKGADITFD
MVTVGGTENILMAAVLAEGTTTIRNAAREPEITDLAQMLIEMGAKIEGLDTDTLVVTGVESLHGCEYSVVADRIETGSYL
AAAAITGGRVKTTHTDPSLLESVLDKFEEMGAEVTRGEDWIELDMMGKRPKAVSFRTLPHPEFPTDMQAQLMAVNVIGRG
FATISETIFENRFMHVPELSRMGANIQVEGNDAIVTGVEKLQAAPVMATDLRASFSLVLAALVAEGDTLIDRIYHIDRGY
ENIEAKLQSLGAKIKRVS

Sequences:

>Translated_418_residues
MDKFLITGGVKLEGEVRISGAKNAALPLLAAMILADSPITLNNVPNLKDVNTLVKLIAGLGITMDYEGETVKADTSTLSN
QFAPYELVKTMRASILVLGPLLARYGNAQVSLPGGCAIGSRPVDQHLKALEALGAHIEVENGYVHATVDGRLKGADITFD
MVTVGGTENILMAAVLAEGTTTIRNAAREPEITDLAQMLIEMGAKIEGLDTDTLVVTGVESLHGCEYSVVADRIETGSYL
AAAAITGGRVKTTHTDPSLLESVLDKFEEMGAEVTRGEDWIELDMMGKRPKAVSFRTLPHPEFPTDMQAQLMAVNVIGRG
FATISETIFENRFMHVPELSRMGANIQVEGNDAIVTGVEKLQAAPVMATDLRASFSLVLAALVAEGDTLIDRIYHIDRGY
ENIEAKLQSLGAKIKRVS
>Mature_418_residues
MDKFLITGGVKLEGEVRISGAKNAALPLLAAMILADSPITLNNVPNLKDVNTLVKLIAGLGITMDYEGETVKADTSTLSN
QFAPYELVKTMRASILVLGPLLARYGNAQVSLPGGCAIGSRPVDQHLKALEALGAHIEVENGYVHATVDGRLKGADITFD
MVTVGGTENILMAAVLAEGTTTIRNAAREPEITDLAQMLIEMGAKIEGLDTDTLVVTGVESLHGCEYSVVADRIETGSYL
AAAAITGGRVKTTHTDPSLLESVLDKFEEMGAEVTRGEDWIELDMMGKRPKAVSFRTLPHPEFPTDMQAQLMAVNVIGRG
FATISETIFENRFMHVPELSRMGANIQVEGNDAIVTGVEKLQAAPVMATDLRASFSLVLAALVAEGDTLIDRIYHIDRGY
ENIEAKLQSLGAKIKRVS

Specific function: Cell wall formation. Adds enolpyruvyl to UDP-N- acetylglucosamine. Target for the antibiotic phosphomycin [H]

COG id: COG0766

COG function: function code M; UDP-N-acetylglucosamine enolpyruvyl transferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family. MurA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789580, Length=418, Percent_Identity=60.2870813397129, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI1787137, Length=410, Percent_Identity=24.8780487804878, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001986
- InterPro:   IPR013792
- InterPro:   IPR005750 [H]

Pfam domain/function: PF00275 EPSP_synthase [H]

EC number: =2.5.1.7 [H]

Molecular weight: Translated: 44760; Mature: 44760

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS50088 ANK_REPEAT ; PS50297 ANK_REP_REGION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKFLITGGVKLEGEVRISGAKNAALPLLAAMILADSPITLNNVPNLKDVNTLVKLIAGL
CCCEEEECCEEEEEEEEECCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHC
GITMDYEGETVKADTSTLSNQFAPYELVKTMRASILVLGPLLARYGNAQVSLPGGCAIGS
CCEEECCCCEEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
RPVDQHLKALEALGAHIEVENGYVHATVDGRLKGADITFDMVTVGGTENILMAAVLAEGT
CCHHHHHHHHHHCCCEEEEECCEEEEEECCEEECCEEEEEEEEECCCCHHHHHHHHHCCC
TTIRNAAREPEITDLAQMLIEMGAKIEGLDTDTLVVTGVESLHGCEYSVVADRIETGSYL
HHHHHCCCCCCHHHHHHHHHHHCCCEECCCCCEEEEECCHHHCCCCHHHHHHHHCCCCEE
AAAAITGGRVKTTHTDPSLLESVLDKFEEMGAEVTRGEDWIELDMMGKRPKAVSFRTLPH
EEEEECCCEEEECCCCHHHHHHHHHHHHHCCCEECCCCCEEEEEECCCCCCCEEEECCCC
PEFPTDMQAQLMAVNVIGRGFATISETIFENRFMHVPELSRMGANIQVEGNDAIVTGVEK
CCCCCCHHHHEEEEEHHHCCHHHHHHHHHHHCCCCCCCHHHCCCEEEECCCCEEEECHHH
LQAAPVMATDLRASFSLVLAALVAEGDTLIDRIYHIDRGYENIEAKLQSLGAKIKRVS
HHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHCHHHCCCC
>Mature Secondary Structure
MDKFLITGGVKLEGEVRISGAKNAALPLLAAMILADSPITLNNVPNLKDVNTLVKLIAGL
CCCEEEECCEEEEEEEEECCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHC
GITMDYEGETVKADTSTLSNQFAPYELVKTMRASILVLGPLLARYGNAQVSLPGGCAIGS
CCEEECCCCEEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
RPVDQHLKALEALGAHIEVENGYVHATVDGRLKGADITFDMVTVGGTENILMAAVLAEGT
CCHHHHHHHHHHCCCEEEEECCEEEEEECCEEECCEEEEEEEEECCCCHHHHHHHHHCCC
TTIRNAAREPEITDLAQMLIEMGAKIEGLDTDTLVVTGVESLHGCEYSVVADRIETGSYL
HHHHHCCCCCCHHHHHHHHHHHCCCEECCCCCEEEEECCHHHCCCCHHHHHHHHCCCCEE
AAAAITGGRVKTTHTDPSLLESVLDKFEEMGAEVTRGEDWIELDMMGKRPKAVSFRTLPH
EEEEECCCEEEECCCCHHHHHHHHHHHHHCCCEECCCCCEEEEEECCCCCCCEEEECCCC
PEFPTDMQAQLMAVNVIGRGFATISETIFENRFMHVPELSRMGANIQVEGNDAIVTGVEK
CCCCCCHHHHEEEEEHHHCCHHHHHHHHHHHCCCCCCCHHHCCCEEEECCCCEEEECHHH
LQAAPVMATDLRASFSLVLAALVAEGDTLIDRIYHIDRGYENIEAKLQSLGAKIKRVS
HHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8181716 [H]