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The map label for this gene is aspS [H]

Identifier: 50083844

GI number: 50083844

Start: 597289

End: 599127

Strand: Reverse

Name: aspS [H]

Synonym: ACIAD0609

Alternate gene names: 50083844

Gene position: 599127-597289 (Counterclockwise)

Preceding gene: 50083845

Following gene: 50083843

Centisome position: 16.65

GC content: 45.24

Gene sequence:

>1839_bases
CTGGCGGTACGCTGTTTACGTATACGCCCCAACAGCCAATATAGTTTGGATTTTTACGACATGATGCGAACTCATTACTG
TGGCTCTTTAACAGAAGCCCAAATTGACCAAACCGTAACCCTATGTGGATGGGTACACCGTCGCCGAGATCATGGTGGTG
TGATTTTCCTTGATATGCGTGACCGCGATGGTTTGGTTCAAGTGGTTATTGACCCAGATACGCCAGAGGCATTTGCGACC
GCAGATAAAGTACGCTCTGAGTTTGTACTGAAAATTACGGGGCGTGTACGTCGCCGCTATGAGGGCACTGAAAATTCAAA
TATGGTCAGTGGTCAAATTGAAGTGTTGGGCAAAGAGATCGAAGTTCTCGCACAATCTGAAACTCCGCCATTTCCATTAA
ATGATGACAATATTAATATTTCTGAAGAGCATCGTTTAAAATACCGTTTCTTGGACATTCGTCGTCCAGAAATGCTAGAT
CGTTTGCGTTTTCGCTCTAAAGTGACCAACCTGATTCGTAACTACCTAGACGATCATGGTTTCTTAGATGTTGAAACCCC
AATCTTAACGCGTGCAACACCTGAAGGCGCACGTGACTATTTGGTTCCAAGCCGCGTACAAAATGGAAGTTTCTATGCTC
TTCCACAATCACCACAGTTGTTCAAACAATTGTTGATGGTGGGCGGTATTGATCGTTACTACCAAATTGCAAAGTGTTTC
CGTGACGAAGACTTGCGTGCAGACCGTCAGCCTGAATTTACCCAGATCGACATCGAAACATCATTCTTAAATGATGATGA
CATTATGGATCTGATGGAAGGCATGACCGTTAAGCTGTTTGACGAATTGCTCGGTATCAAATTCGACAAGTTCCAGCGTA
TGCCATACAGCGAAGCGATGCGTGACTATGCATCGGACAAACCGGACTTACGTATTCCGCTTAAACTTGTTGATGTTGCA
GATCTGATGCAAGACGTCGAGTTTAAAGTATTTGCGGGTCCAGCCAAAGATCCTAAAGGCCGTATTGCAGCACTTCGCGT
GCCGGGCGCAGGCGCTTTGCCACGTAGTGCGATTGATGAATACACCAAATTTGTGGGCATTTATGGCGCTAAAGGCCTCG
CTTACATCAAGGTCAATGAAATTGAAAAAGGCGTAGAAGGCTTACAGTCTCCAATCGTGAAATTCATTGAACCGATCGTG
ATGCAATTGCTTGAGCGTGTAGGCGCAGAAAATGGCGATATCGTCTTCTTTGGTGCAGATAAAGCCAAAGTGGTAAATGA
TGCCATGGGTGCACTTCGTGTGAAAATCGGTCACGACCTTAAACTTGTAACCTGTGAATGGGCGCCGCTTTGGGTGGTCG
ACTTCCCAATGTTTGAAGAAACAGATGATGGTAAATGGACTTCAGTTCACCATCCATTTACGCTGCCAAAATCAAGCGTT
GAAGATGTGAAAGCAAATCCGGGTGAAGCACTTTCTGTTGCTTATGACATGGTCTTGAATGGTACCGAAGTCGGTGGTGG
TTCATTACGTATCTATACGCTTGAAATGCAAAAAGCCATCTTTGAAGCATTGGGTATTTCAGACGAAGAAGCCGAAGAGA
AGTTTAGCTTCTTACTGAATGCCTTGCGTTATGGCGCGCCTCCGCATGGTGGTTTGGCATTTGGTCTAGATCGTTTAATC
ATGCTCATGACTGGTGCATCTTCAATTCGTGATGTGATTGCATTCCCGAAAACCAAGACTGCTGAATGTCCACTGACTCA
GGCACCAGCACCAGTTGAAGCGAATCAACTACGTGATTTGGGTATTCGTTTACGCGAACAGCCAAAGAAAGAAGACTAA

Upstream 100 bases:

>100_bases
CACAAAGTTGATTAAATCCGTTCAAAAATAGCGCATTTTTTGCAGTTTTTGGCGCTAGTTCTTGTGACCCGCCACTCAAA
AAGGCATAATCGCTGATTCA

Downstream 100 bases:

>100_bases
TCTCATCATAAAGTACCTAAAAAAGCTAGCAGATATGCTGGCTTTTTTGCATTTTAATGTCTAGTCTCACAACAATAATG
CGACATGAATTGAGATTGAT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 612; Mature: 611

Protein sequence:

>612_residues
MAVRCLRIRPNSQYSLDFYDMMRTHYCGSLTEAQIDQTVTLCGWVHRRRDHGGVIFLDMRDRDGLVQVVIDPDTPEAFAT
ADKVRSEFVLKITGRVRRRYEGTENSNMVSGQIEVLGKEIEVLAQSETPPFPLNDDNINISEEHRLKYRFLDIRRPEMLD
RLRFRSKVTNLIRNYLDDHGFLDVETPILTRATPEGARDYLVPSRVQNGSFYALPQSPQLFKQLLMVGGIDRYYQIAKCF
RDEDLRADRQPEFTQIDIETSFLNDDDIMDLMEGMTVKLFDELLGIKFDKFQRMPYSEAMRDYASDKPDLRIPLKLVDVA
DLMQDVEFKVFAGPAKDPKGRIAALRVPGAGALPRSAIDEYTKFVGIYGAKGLAYIKVNEIEKGVEGLQSPIVKFIEPIV
MQLLERVGAENGDIVFFGADKAKVVNDAMGALRVKIGHDLKLVTCEWAPLWVVDFPMFEETDDGKWTSVHHPFTLPKSSV
EDVKANPGEALSVAYDMVLNGTEVGGGSLRIYTLEMQKAIFEALGISDEEAEEKFSFLLNALRYGAPPHGGLAFGLDRLI
MLMTGASSIRDVIAFPKTKTAECPLTQAPAPVEANQLRDLGIRLREQPKKED

Sequences:

>Translated_612_residues
MAVRCLRIRPNSQYSLDFYDMMRTHYCGSLTEAQIDQTVTLCGWVHRRRDHGGVIFLDMRDRDGLVQVVIDPDTPEAFAT
ADKVRSEFVLKITGRVRRRYEGTENSNMVSGQIEVLGKEIEVLAQSETPPFPLNDDNINISEEHRLKYRFLDIRRPEMLD
RLRFRSKVTNLIRNYLDDHGFLDVETPILTRATPEGARDYLVPSRVQNGSFYALPQSPQLFKQLLMVGGIDRYYQIAKCF
RDEDLRADRQPEFTQIDIETSFLNDDDIMDLMEGMTVKLFDELLGIKFDKFQRMPYSEAMRDYASDKPDLRIPLKLVDVA
DLMQDVEFKVFAGPAKDPKGRIAALRVPGAGALPRSAIDEYTKFVGIYGAKGLAYIKVNEIEKGVEGLQSPIVKFIEPIV
MQLLERVGAENGDIVFFGADKAKVVNDAMGALRVKIGHDLKLVTCEWAPLWVVDFPMFEETDDGKWTSVHHPFTLPKSSV
EDVKANPGEALSVAYDMVLNGTEVGGGSLRIYTLEMQKAIFEALGISDEEAEEKFSFLLNALRYGAPPHGGLAFGLDRLI
MLMTGASSIRDVIAFPKTKTAECPLTQAPAPVEANQLRDLGIRLREQPKKED
>Mature_611_residues
AVRCLRIRPNSQYSLDFYDMMRTHYCGSLTEAQIDQTVTLCGWVHRRRDHGGVIFLDMRDRDGLVQVVIDPDTPEAFATA
DKVRSEFVLKITGRVRRRYEGTENSNMVSGQIEVLGKEIEVLAQSETPPFPLNDDNINISEEHRLKYRFLDIRRPEMLDR
LRFRSKVTNLIRNYLDDHGFLDVETPILTRATPEGARDYLVPSRVQNGSFYALPQSPQLFKQLLMVGGIDRYYQIAKCFR
DEDLRADRQPEFTQIDIETSFLNDDDIMDLMEGMTVKLFDELLGIKFDKFQRMPYSEAMRDYASDKPDLRIPLKLVDVAD
LMQDVEFKVFAGPAKDPKGRIAALRVPGAGALPRSAIDEYTKFVGIYGAKGLAYIKVNEIEKGVEGLQSPIVKFIEPIVM
QLLERVGAENGDIVFFGADKAKVVNDAMGALRVKIGHDLKLVTCEWAPLWVVDFPMFEETDDGKWTSVHHPFTLPKSSVE
DVKANPGEALSVAYDMVLNGTEVGGGSLRIYTLEMQKAIFEALGISDEEAEEKFSFLLNALRYGAPPHGGLAFGLDRLIM
LMTGASSIRDVIAFPKTKTAECPLTQAPAPVEANQLRDLGIRLREQPKKED

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=600, Percent_Identity=39.6666666666667, Blast_Score=404, Evalue=1e-112,
Organism=Homo sapiens, GI5031815, Length=298, Percent_Identity=25.503355704698, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI194272210, Length=298, Percent_Identity=25.503355704698, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI45439306, Length=272, Percent_Identity=26.1029411764706, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI4758762, Length=259, Percent_Identity=27.7992277992278, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI226491502, Length=255, Percent_Identity=27.4509803921569, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1788173, Length=586, Percent_Identity=58.7030716723549, Blast_Score=725, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=291, Percent_Identity=25.085910652921, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1790571, Length=293, Percent_Identity=25.5972696245734, Blast_Score=80, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI32566633, Length=627, Percent_Identity=37.1610845295056, Blast_Score=347, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI17535927, Length=306, Percent_Identity=26.4705882352941, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71994340, Length=306, Percent_Identity=26.4705882352941, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17535925, Length=269, Percent_Identity=27.1375464684015, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17551876, Length=269, Percent_Identity=25.6505576208178, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71984122, Length=292, Percent_Identity=25.6849315068493, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=643, Percent_Identity=31.7262830482115, Blast_Score=281, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6320242, Length=271, Percent_Identity=26.5682656826568, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323011, Length=270, Percent_Identity=26.6666666666667, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321807, Length=253, Percent_Identity=25.296442687747, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324256, Length=213, Percent_Identity=25.8215962441315, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24584738, Length=599, Percent_Identity=38.3973288814691, Blast_Score=346, Evalue=2e-95,
Organism=Drosophila melanogaster, GI17136276, Length=235, Percent_Identity=29.7872340425532, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24640849, Length=268, Percent_Identity=27.6119402985075, Blast_Score=90, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24640851, Length=268, Percent_Identity=27.6119402985075, Blast_Score=90, Evalue=6e-18,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 69077; Mature: 68946

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVRCLRIRPNSQYSLDFYDMMRTHYCGSLTEAQIDQTVTLCGWVHRRRDHGGVIFLDMR
CCEEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
DRDGLVQVVIDPDTPEAFATADKVRSEFVLKITGRVRRRYEGTENSNMVSGQIEVLGKEI
CCCCEEEEEECCCCCHHHHHHHHHHHHEEEEEHHHHHHHCCCCCCCCEEECHHHHHHHHH
EVLAQSETPPFPLNDDNINISEEHRLKYRFLDIRRPEMLDRLRFRSKVTNLIRNYLDDHG
HHEECCCCCCCCCCCCCCCCCHHHCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCC
FLDVETPILTRATPEGARDYLVPSRVQNGSFYALPQSPQLFKQLLMVGGIDRYYQIAKCF
EEEECCCEEECCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHH
RDEDLRADRQPEFTQIDIETSFLNDDDIMDLMEGMTVKLFDELLGIKFDKFQRMPYSEAM
CCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHCCCHHHHHHHHHCCCHHHHHCCCHHHHH
RDYASDKPDLRIPLKLVDVADLMQDVEFKVFAGPAKDPKGRIAALRVPGAGALPRSAIDE
HHHHCCCCCCEECHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHH
YTKFVGIYGAKGLAYIKVNEIEKGVEGLQSPIVKFIEPIVMQLLERVGAENGDIVFFGAD
HHHHHHEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
KAKVVNDAMGALRVKIGHDLKLVTCEWAPLWVVDFPMFEETDDGKWTSVHHPFTLPKSSV
HHHHHHHHHHHEEEEECCCEEEEEECCCCEEEEECCCCCCCCCCCEEEECCCCCCCCCCH
EDVKANPGEALSVAYDMVLNGTEVGGGSLRIYTLEMQKAIFEALGISDEEAEEKFSFLLN
HHCCCCCCCHHHHEEHHHCCCCEECCCEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHH
ALRYGAPPHGGLAFGLDRLIMLMTGASSIRDVIAFPKTKTAECPLTQAPAPVEANQLRDL
HHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
GIRLREQPKKED
HHHHHCCCCCCC
>Mature Secondary Structure 
AVRCLRIRPNSQYSLDFYDMMRTHYCGSLTEAQIDQTVTLCGWVHRRRDHGGVIFLDMR
CEEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
DRDGLVQVVIDPDTPEAFATADKVRSEFVLKITGRVRRRYEGTENSNMVSGQIEVLGKEI
CCCCEEEEEECCCCCHHHHHHHHHHHHEEEEEHHHHHHHCCCCCCCCEEECHHHHHHHHH
EVLAQSETPPFPLNDDNINISEEHRLKYRFLDIRRPEMLDRLRFRSKVTNLIRNYLDDHG
HHEECCCCCCCCCCCCCCCCCHHHCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCC
FLDVETPILTRATPEGARDYLVPSRVQNGSFYALPQSPQLFKQLLMVGGIDRYYQIAKCF
EEEECCCEEECCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHH
RDEDLRADRQPEFTQIDIETSFLNDDDIMDLMEGMTVKLFDELLGIKFDKFQRMPYSEAM
CCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHCCCHHHHHHHHHCCCHHHHHCCCHHHHH
RDYASDKPDLRIPLKLVDVADLMQDVEFKVFAGPAKDPKGRIAALRVPGAGALPRSAIDE
HHHHCCCCCCEECHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHH
YTKFVGIYGAKGLAYIKVNEIEKGVEGLQSPIVKFIEPIVMQLLERVGAENGDIVFFGAD
HHHHHHEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
KAKVVNDAMGALRVKIGHDLKLVTCEWAPLWVVDFPMFEETDDGKWTSVHHPFTLPKSSV
HHHHHHHHHHHEEEEECCCEEEEEECCCCEEEEECCCCCCCCCCCEEEECCCCCCCCCCH
EDVKANPGEALSVAYDMVLNGTEVGGGSLRIYTLEMQKAIFEALGISDEEAEEKFSFLLN
HHCCCCCCCHHHHEEHHHCCCCEECCCEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHH
ALRYGAPPHGGLAFGLDRLIMLMTGASSIRDVIAFPKTKTAECPLTQAPAPVEANQLRDL
HHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
GIRLREQPKKED
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA