Click here to switch to the map view.

The map label for this gene is ilvE [H]

Identifier: 50083832

GI number: 50083832

Start: 587193

End: 588128

Strand: Direct

Name: ilvE [H]

Synonym: ACIAD0597

Alternate gene names: 50083832

Gene position: 587193-588128 (Clockwise)

Preceding gene: 50083831

Following gene: 50083833

Centisome position: 16.32

GC content: 43.27

Gene sequence:

>936_bases
GTGTGTGCCATGAATTTGGCTGATCGTGATGGTTTTATTTGGCAAGATGGACAATTGGTTGACTGGCGTGATGCAAAAAT
TCACGTTTTAACTCATACGCTACACTACAGTATGGGTGTCTTTGAAGGTGTCCGTGCCTATGAAACTCCTAAAGGCACTG
CAATTTTTCGTCTCCAAGATCACACTAAGCGTTTGTTAAACTCTGCAAAAATTTATCAAATGAAAGTTCCATTTGATCAA
GCGACACTTGAGCAAGCTCAAATTGATGTGGTTCGTGAAAATAAATTAGCATCTTGTTACTTGCGTCCATTGATCTGGAT
CGGTTCTGAAAAATTAGGTATTGCTGCCACTGAAAATAGTATTCATGCTGCCGTTGCGGCATGGGGCTGGGGTGCTTACC
TTGGTGAAGAGGCAATGGCACGTGGTATTCGTGTAAAAACATCTTCGTTTACGCATCATCATCCAAACGTGACGATGTGT
AAGGCAAAAGCATGTGGCAACTATACTGTGTCGATCTTGGCACATCAGGAAGTAGCGCGTTCAGGTTATGATGAAGCGAT
GTTGCTAGATCCACAAGGCTTTGTGTGTCAAGGTGCAGGCGAGAACGTGTTCTTGATTAAAGATGGCGTGTTGCATACCC
CTGATTTGGCTGGTGGTGCATTAGAAGGGATTACCCGTCAAACAGTTATTACCATTGCAAAAGATTTAGGATACGAAGTG
GTTGAGCGTCGTATTACCCGTGATGAATTCTACATTGCAGATGAAGCATTCTTTACTGGTACTGCCGCAGAAGTGACGCC
AATTCGTGAATACGATGACCGTGAAATTGGTGAAGGATGCCGTGGCCCAATTACCACAGAAATCCAGAAAACCTTTTTCG
ATGCGGTTCAGGGCAAAAATGCAAAATATGAACACTGGTTAACTTACGTGAAGTAA

Upstream 100 bases:

>100_bases
GGCGCCATACCCGAGAGGTCGTTTGCAAGTTATGGCAAAGATTAATAGATCCAGCGTCTACAATGGCGTTGGAGAGTGAA
TGATTGTGTAAAAATTTGGA

Downstream 100 bases:

>100_bases
GCGAATCCGTTAAGATCAAGGCGAAACACATGTTTCGCCTTTTTTATTGATGTTTTTAGGAAAAAATATGCATGTTGTGT
ATGTGAGTGATGGAAAAGCA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MCAMNLADRDGFIWQDGQLVDWRDAKIHVLTHTLHYSMGVFEGVRAYETPKGTAIFRLQDHTKRLLNSAKIYQMKVPFDQ
ATLEQAQIDVVRENKLASCYLRPLIWIGSEKLGIAATENSIHAAVAAWGWGAYLGEEAMARGIRVKTSSFTHHHPNVTMC
KAKACGNYTVSILAHQEVARSGYDEAMLLDPQGFVCQGAGENVFLIKDGVLHTPDLAGGALEGITRQTVITIAKDLGYEV
VERRITRDEFYIADEAFFTGTAAEVTPIREYDDREIGEGCRGPITTEIQKTFFDAVQGKNAKYEHWLTYVK

Sequences:

>Translated_311_residues
MCAMNLADRDGFIWQDGQLVDWRDAKIHVLTHTLHYSMGVFEGVRAYETPKGTAIFRLQDHTKRLLNSAKIYQMKVPFDQ
ATLEQAQIDVVRENKLASCYLRPLIWIGSEKLGIAATENSIHAAVAAWGWGAYLGEEAMARGIRVKTSSFTHHHPNVTMC
KAKACGNYTVSILAHQEVARSGYDEAMLLDPQGFVCQGAGENVFLIKDGVLHTPDLAGGALEGITRQTVITIAKDLGYEV
VERRITRDEFYIADEAFFTGTAAEVTPIREYDDREIGEGCRGPITTEIQKTFFDAVQGKNAKYEHWLTYVK
>Mature_311_residues
MCAMNLADRDGFIWQDGQLVDWRDAKIHVLTHTLHYSMGVFEGVRAYETPKGTAIFRLQDHTKRLLNSAKIYQMKVPFDQ
ATLEQAQIDVVRENKLASCYLRPLIWIGSEKLGIAATENSIHAAVAAWGWGAYLGEEAMARGIRVKTSSFTHHHPNVTMC
KAKACGNYTVSILAHQEVARSGYDEAMLLDPQGFVCQGAGENVFLIKDGVLHTPDLAGGALEGITRQTVITIAKDLGYEV
VERRITRDEFYIADEAFFTGTAAEVTPIREYDDREIGEGCRGPITTEIQKTFFDAVQGKNAKYEHWLTYVK

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=301, Percent_Identity=45.514950166113, Blast_Score=280, Evalue=6e-77,
Organism=Caenorhabditis elegans, GI17565728, Length=324, Percent_Identity=26.5432098765432, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322002, Length=306, Percent_Identity=25.8169934640523, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322608, Length=285, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34765; Mature: 34765

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCAMNLADRDGFIWQDGQLVDWRDAKIHVLTHTLHYSMGVFEGVRAYETPKGTAIFRLQD
CCCCCCCCCCCEEEECCCEEECCCCEEEEEEEEHHHHHHHHHCHHHHCCCCCCEEEEECH
HTKRLLNSAKIYQMKVPFDQATLEQAQIDVVRENKLASCYLRPLIWIGSEKLGIAATENS
HHHHHHCCCEEEEEECCCCHHHHHHHHHEEHHCCCHHHHHHCEEEEECCCCEEEEEECCC
IHAAVAAWGWGAYLGEEAMARGIRVKTSSFTHHHPNVTMCKAKACGNYTVSILAHQEVAR
CEEEEEHHCHHHHHCHHHHHCCEEEEECCCEECCCCEEEEEECCCCCEEEEEEEHHHHHH
SGYDEAMLLDPQGFVCQGAGENVFLIKDGVLHTPDLAGGALEGITRQTVITIAKDLGYEV
CCCCCEEEECCCCCEEECCCCEEEEEECCCEECCCCCCHHHHCCHHHHHHHHHHHCCHHH
VERRITRDEFYIADEAFFTGTAAEVTPIREYDDREIGEGCRGPITTEIQKTFFDAVQGKN
HHHHHCCCCEEEECCCEECCCCCEECCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHCCCC
AKYEHWLTYVK
CCHHHHEEECC
>Mature Secondary Structure
MCAMNLADRDGFIWQDGQLVDWRDAKIHVLTHTLHYSMGVFEGVRAYETPKGTAIFRLQD
CCCCCCCCCCCEEEECCCEEECCCCEEEEEEEEHHHHHHHHHCHHHHCCCCCCEEEEECH
HTKRLLNSAKIYQMKVPFDQATLEQAQIDVVRENKLASCYLRPLIWIGSEKLGIAATENS
HHHHHHCCCEEEEEECCCCHHHHHHHHHEEHHCCCHHHHHHCEEEEECCCCEEEEEECCC
IHAAVAAWGWGAYLGEEAMARGIRVKTSSFTHHHPNVTMCKAKACGNYTVSILAHQEVAR
CEEEEEHHCHHHHHCHHHHHCCEEEEECCCEECCCCEEEEEECCCCCEEEEEEEHHHHHH
SGYDEAMLLDPQGFVCQGAGENVFLIKDGVLHTPDLAGGALEGITRQTVITIAKDLGYEV
CCCCCEEEECCCCCEEECCCCEEEEEECCCEECCCCCCHHHHCCHHHHHHHHHHHCCHHH
VERRITRDEFYIADEAFFTGTAAEVTPIREYDDREIGEGCRGPITTEIQKTFFDAVQGKN
HHHHHCCCCEEEECCCEECCCCCEECCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHCCCC
AKYEHWLTYVK
CCHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]