Click here to switch to the map view.

The map label for this gene is entF [C]

Identifier: 50083807

GI number: 50083807

Start: 560611

End: 562278

Strand: Direct

Name: entF [C]

Synonym: ACIAD0571

Alternate gene names: 50083807

Gene position: 560611-562278 (Clockwise)

Preceding gene: 50083806

Following gene: 50083808

Centisome position: 15.58

GC content: 45.74

Gene sequence:

>1668_bases
TTGAATGATTTTATTCGGTCACCCCGAACCTTGGCGATCAATGCTCAACTAAAAGCAATTTCTTTTGCAGAGTTTTGGCA
GGATGTTGCAGCACAGGCGGCCTGGATTCAACCATCCCCCCAATCTGTCTGGGCGTTATGGGAACAGGACAGTTATGATT
TTTTAGTCCTGTTATTTGCAGGGCTGGTCGCCAAGAAACGTATCCTTCTTCCACCAAATCGTGTCGCACAGCTAGAACAA
GACTTTCGTAGGCAGGACATTCACTTTCTCTCACGTCAGTTGAGTATTTCACCAGTACAGATGATACCAATTCCTGAATG
GAACACTGAAACACTCAATCAGGCACATATCGATTTTTATACATCAGGTTCGACAGGACAGCCCAAACGTATTGAGCGAA
ATTTGGCGCAATTACTCTCTGAAGTACAGGGACTAGACCAGACTTTTGGTTTAGCCGAGAACAGCATTGCGCTGGCAACT
GTGAGTCACCAGCATATCTATGGTTTACTGTTTAAAGTGCTCTGGCCACTTGCCACGGGACGATGTTTTTACAGTCCACA
ATTGGGATTCCCGGAAGATGTGGTGATCATGCAACAGCGCTTCACTACGTCTAATAGGCAAAATTATGTGATCTCCAGTC
CAGCATTATTAAAACGCTGGAATAAAGATATGGTATTGCAAGCGTGTGAAGCTGTGTTTTCTTCAGGTGGCAAGCTGGAG
TCTGGAATCCGACCTCAACTGAATGATGCCATTACTGAAATTCTGGGAAGCTCCGAGACGGGTGGTATTGCTTATCGACA
TCAGGATCATGCCGCATGGCAGGCTTTTGCTGATGTTGATATCCAGATTGAGCAAGCACATTTGCAGGTTAAAACGGCGC
ATGCTTATACCGAGCAATGGATTGAAACAGGCGATTTGGCACAATGGCAGCAATTAGACAATACCACACTCGGCTTTGAA
CTTTTAGGTCGTGCAGATCGTTTAATCAAGCTTGAAGAAAAGCGGCTGAGTCTGGATGCAATTGAGCAGGGGATTCAGTC
ACAACCTCAGGTTGAGCAGTGTCATGCCTTGGTGGTTGAGCATGAGCAACGGCAGCTTCTCGCCTGCATCGTGGTACTCA
ACGCTGATGCACGTGCATCTTTAAAGCAGTTGGGCAAGCGCCAGTTTGTATCGCAGTTAAAACACCAGTTATCCCATGGT
CTTGAGCGCATTGCGATTCCGCGTCTTTGGCGTTTTCTCACGGATATTCCGCACAATAGTCAGTCCAAGCGAGATAAACA
TTATTTAAAAAGTTTGTTTCAGCCGATGCTATATCCCGTTGTTTTACAACGTACAGCACTTGAAAACGAGCATTGCTGGA
CACTTGAATTTTCACCAGAACTGGAATGTTTTAAAGGGCATTTCCCAACACAACCGATTTATCCTGGTGTCGGGCAGATT
GGTTTTGTTGAGGCTTTTGCCAAACAGATTTGGGCCGATCTGGACTGGTGTAGTGGCTATGAGCAACTTAAGTTTCAGCA
TGTGATTGTGCCTTATAGTGTGATCGCGATGAAATTGACACGTAAGCAACATAAAATCAGTTTCGAGCTGCACCAGCAAG
ATCAAACCATGGCATCTGGACGCTTGCTATTTGCGCTAAAGATTGCAGGTGAGGAGCGTTCGACATGA

Upstream 100 bases:

>100_bases
AATGGTTTTATCTCCTATGTGTTGATGGGGATTCTTTTACTTGGCGAATTTATATTAAGAAAACGTTATCGACGTTTACA
CCATAATGATGAACAATCAC

Downstream 100 bases:

>100_bases
CAGCTTATGGTTTTATTATTCCAGTGTTTAATCATCCGCACTATCTGGATCAGTTGGTGGAAACTTTAAGCCGTTATCAA
TTGCCTATGATTTTGGTTAA

Product: hypothetical protein

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: CoA Ligase; Acyl-Co A Synthetase; AMP-(Fatty) Acid Ligase; Surfactin Synthetase; Acyl-CoA Synthetase; Acyl-Co A Synthetase-Related Protein; Aconitate Hydratase; Fatty Acid Degradation; Acyl-Co A Synthetase/AMP-(Fatty) Acid Ligase; Trigger Factor; Acyl-Co A Synthetase/AMP-(Fatty) Acid Ligase Related Enzyme; 3-Phosphoshikimate 1-Carboxyvinyltransferase; Fatty Acid CoA Ligase; AMP-(Fatty)Acid Ligase; Acetyl-Co A Synthetase; Acyl-Co A Synthetases/AMP-(Fatty) Acid Ligase; Acyl-Co A Synthetases/AMP-(Fatty) Acid Ligases-Like Protein

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MNDFIRSPRTLAINAQLKAISFAEFWQDVAAQAAWIQPSPQSVWALWEQDSYDFLVLLFAGLVAKKRILLPPNRVAQLEQ
DFRRQDIHFLSRQLSISPVQMIPIPEWNTETLNQAHIDFYTSGSTGQPKRIERNLAQLLSEVQGLDQTFGLAENSIALAT
VSHQHIYGLLFKVLWPLATGRCFYSPQLGFPEDVVIMQQRFTTSNRQNYVISSPALLKRWNKDMVLQACEAVFSSGGKLE
SGIRPQLNDAITEILGSSETGGIAYRHQDHAAWQAFADVDIQIEQAHLQVKTAHAYTEQWIETGDLAQWQQLDNTTLGFE
LLGRADRLIKLEEKRLSLDAIEQGIQSQPQVEQCHALVVEHEQRQLLACIVVLNADARASLKQLGKRQFVSQLKHQLSHG
LERIAIPRLWRFLTDIPHNSQSKRDKHYLKSLFQPMLYPVVLQRTALENEHCWTLEFSPELECFKGHFPTQPIYPGVGQI
GFVEAFAKQIWADLDWCSGYEQLKFQHVIVPYSVIAMKLTRKQHKISFELHQQDQTMASGRLLFALKIAGEERST

Sequences:

>Translated_555_residues
MNDFIRSPRTLAINAQLKAISFAEFWQDVAAQAAWIQPSPQSVWALWEQDSYDFLVLLFAGLVAKKRILLPPNRVAQLEQ
DFRRQDIHFLSRQLSISPVQMIPIPEWNTETLNQAHIDFYTSGSTGQPKRIERNLAQLLSEVQGLDQTFGLAENSIALAT
VSHQHIYGLLFKVLWPLATGRCFYSPQLGFPEDVVIMQQRFTTSNRQNYVISSPALLKRWNKDMVLQACEAVFSSGGKLE
SGIRPQLNDAITEILGSSETGGIAYRHQDHAAWQAFADVDIQIEQAHLQVKTAHAYTEQWIETGDLAQWQQLDNTTLGFE
LLGRADRLIKLEEKRLSLDAIEQGIQSQPQVEQCHALVVEHEQRQLLACIVVLNADARASLKQLGKRQFVSQLKHQLSHG
LERIAIPRLWRFLTDIPHNSQSKRDKHYLKSLFQPMLYPVVLQRTALENEHCWTLEFSPELECFKGHFPTQPIYPGVGQI
GFVEAFAKQIWADLDWCSGYEQLKFQHVIVPYSVIAMKLTRKQHKISFELHQQDQTMASGRLLFALKIAGEERST
>Mature_555_residues
MNDFIRSPRTLAINAQLKAISFAEFWQDVAAQAAWIQPSPQSVWALWEQDSYDFLVLLFAGLVAKKRILLPPNRVAQLEQ
DFRRQDIHFLSRQLSISPVQMIPIPEWNTETLNQAHIDFYTSGSTGQPKRIERNLAQLLSEVQGLDQTFGLAENSIALAT
VSHQHIYGLLFKVLWPLATGRCFYSPQLGFPEDVVIMQQRFTTSNRQNYVISSPALLKRWNKDMVLQACEAVFSSGGKLE
SGIRPQLNDAITEILGSSETGGIAYRHQDHAAWQAFADVDIQIEQAHLQVKTAHAYTEQWIETGDLAQWQQLDNTTLGFE
LLGRADRLIKLEEKRLSLDAIEQGIQSQPQVEQCHALVVEHEQRQLLACIVVLNADARASLKQLGKRQFVSQLKHQLSHG
LERIAIPRLWRFLTDIPHNSQSKRDKHYLKSLFQPMLYPVVLQRTALENEHCWTLEFSPELECFKGHFPTQPIYPGVGQI
GFVEAFAKQIWADLDWCSGYEQLKFQHVIVPYSVIAMKLTRKQHKISFELHQQDQTMASGRLLFALKIAGEERST

Specific function: Activates The Carboxylate Group Of L-Serine Via ATP- Dependent Ppi Exchange Reactions To The Aminoacyladenylate, Preparing That Molecule For The Final Stages Of Enterobactin Synthesis. Holo-Entf Acts As The Catalyst For The Formation Of The Three Amide An

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.- [C]

Molecular weight: Translated: 63397; Mature: 63397

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDFIRSPRTLAINAQLKAISFAEFWQDVAAQAAWIQPSPQSVWALWEQDSYDFLVLLFA
CCCCCCCCCEEEEECEEHHHHHHHHHHHHHHHEEECCCCCHHHHEEECCCCCHHHHHHHH
GLVAKKRILLPPNRVAQLEQDFRRQDIHFLSRQLSISPVQMIPIPEWNTETLNQAHIDFY
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEE
TSGSTGQPKRIERNLAQLLSEVQGLDQTFGLAENSIALATVSHQHIYGLLFKVLWPLATG
ECCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHCC
RCFYSPQLGFPEDVVIMQQRFTTSNRQNYVISSPALLKRWNKDMVLQACEAVFSSGGKLE
CEEECCCCCCCHHHHHHHHHHCCCCCCCEEECCCHHHHHCCHHHHHHHHHHHHHCCCCCC
SGIRPQLNDAITEILGSSETGGIAYRHQDHAAWQAFADVDIQIEQAHLQVKTAHAYTEQW
CCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHCCEEEEEEEHEEEEHHHHHHHHH
IETGDLAQWQQLDNTTLGFELLGRADRLIKLEEKRLSLDAIEQGIQSQPQVEQCHALVVE
HCCCCHHHHHHHCCCHHHHHHHCCHHHHEEHHHHHCCHHHHHHCCCCCCCHHHHHHHHHH
HEQRQLLACIVVLNADARASLKQLGKRQFVSQLKHQLSHGLERIAIPRLWRFLTDIPHNS
HHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QSKRDKHYLKSLFQPMLYPVVLQRTALENEHCWTLEFSPELECFKGHFPTQPIYPGVGQI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCCCCCCCCCCCCCH
GFVEAFAKQIWADLDWCSGYEQLKFQHVIVPYSVIAMKLTRKQHKISFELHQQDQTMASG
HHHHHHHHHHHCCCHHHCCHHHHCEEEEECHHHHHHHHHHHHHHHEEEEEHHHHHHHHCC
RLLFALKIAGEERST
EEEEEEEECCCCCCC
>Mature Secondary Structure
MNDFIRSPRTLAINAQLKAISFAEFWQDVAAQAAWIQPSPQSVWALWEQDSYDFLVLLFA
CCCCCCCCCEEEEECEEHHHHHHHHHHHHHHHEEECCCCCHHHHEEECCCCCHHHHHHHH
GLVAKKRILLPPNRVAQLEQDFRRQDIHFLSRQLSISPVQMIPIPEWNTETLNQAHIDFY
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEE
TSGSTGQPKRIERNLAQLLSEVQGLDQTFGLAENSIALATVSHQHIYGLLFKVLWPLATG
ECCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHCC
RCFYSPQLGFPEDVVIMQQRFTTSNRQNYVISSPALLKRWNKDMVLQACEAVFSSGGKLE
CEEECCCCCCCHHHHHHHHHHCCCCCCCEEECCCHHHHHCCHHHHHHHHHHHHHCCCCCC
SGIRPQLNDAITEILGSSETGGIAYRHQDHAAWQAFADVDIQIEQAHLQVKTAHAYTEQW
CCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHCCEEEEEEEHEEEEHHHHHHHHH
IETGDLAQWQQLDNTTLGFELLGRADRLIKLEEKRLSLDAIEQGIQSQPQVEQCHALVVE
HCCCCHHHHHHHCCCHHHHHHHCCHHHHEEHHHHHCCHHHHHHCCCCCCCHHHHHHHHHH
HEQRQLLACIVVLNADARASLKQLGKRQFVSQLKHQLSHGLERIAIPRLWRFLTDIPHNS
HHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QSKRDKHYLKSLFQPMLYPVVLQRTALENEHCWTLEFSPELECFKGHFPTQPIYPGVGQI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCCCCCCCCCCCCCH
GFVEAFAKQIWADLDWCSGYEQLKFQHVIVPYSVIAMKLTRKQHKISFELHQQDQTMASG
HHHHHHHHHHHCCCHHHCCHHHHCEEEEECHHHHHHHHHHHHHHHEEEEEHHHHHHHHCC
RLLFALKIAGEERST
EEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA