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The map label for this gene is hisS

Identifier: 50083798

GI number: 50083798

Start: 553181

End: 554473

Strand: Direct

Name: hisS

Synonym: ACIAD0562

Alternate gene names: 50083798

Gene position: 553181-554473 (Clockwise)

Preceding gene: 50083797

Following gene: 50083799

Centisome position: 15.37

GC content: 43.85

Gene sequence:

>1293_bases
ATGAGTTCAATTGTCGCAATAAAAGGTTTTAATGACGTTCTTCCTACTCAAACAGCAGCATGGAGACGTCTTGAGCAACA
TCTCGCATCCTTGATGGATGCTTACGGTTATCAGCAAATTCGTTTGCCAATCGTGGAGCAGACAGGACTTTTCAAGCGTG
CCATTGGTGATGCGACAGATATTGTTGAAAAAGAAATGTATACCTTTTTTGACAAAGGAACACCTCCCGAATCACTTACA
CTACGTCCCGAAGGGACTGCAGGCTGTGTGCGCGCAATGTTGGAACATAACCTTCTGCGTGGCGCAACACCGCGTGTCTG
GTATGTGGGGCCAATGTTTCGCTATGAAAAACCACAAAAAGGTCGCTATCGTCAGTTTCATCAGTTTGGTGTAGAAACTT
TTGGTGTGGCAACGCCAGACATCGATGCAGAATTGATTTTGATGACAGCACGCCTCTGGAAACGTATGGGTGTTTCAGAG
AAGGTTCAACTCGAACTCAACACGCTAGGTGAAATAGATGAACGTGCAGCCTATCGTACGGCGTTGGTTGAATTCTTAAC
TCAGCATAAAGAGGCTTTAGATGAGGATTCACAGCGCCGTCTTGGCACCAACCCTCTGCGTATTCTGGATTCTAAAGTTG
AGTCTACTCAAAAAATTTTAGAAAATGCACCCAAGCTGCATGATTTTCTTCAAGAAGATTCGTTAGCGCATTTTAATCAG
TTGCAAGAATACCTAACCCATGCAGGCGTATCATTTGTAATTAATCAAAAACTGGTTCGAGGTCTTGATTATTACAACAA
AACTGTATTTGAATGGACCACCACTGCACTGGGTTCTCAAGGTACGGTGTGTGCTGGCGGTCGCTATGATGGTTTGGTCG
GACAGCTCAAGGGTAAAGCGGATCAATCGGTTCCTGCTGTCGGTTTTGCGATGGGTATGGAGCGTTTATTATTGCTGCTT
GAGCAAGTTGAACAGGCAGAAGTAGTTCGTGATTGTGATGTGTTTTTAGTCGCAGAGTCTGCTTTTCAAGGACATGCACT
GGTTTTGGCTGAACAGATTCGTGATCAGTTCGAAGGATTAGCAAGTACAATCCGAGTTAAAACAGGTTCGCAAGGTAGTA
TGAAAAGCCAGATGAAAAAAGCGGATCAGTCGGGTGCGCATTATGCTGTAATTTTGGGTGAGAGAGAGTGGACTACCCAA
GAATTGACAGTAAAAGAGCTAGCAACGTCTGAGCAATCTCAAGTCGCTATTTCGGAACTTGTACCGTTTTTAGTTAAAAA
ATTTGAAAAATAA

Upstream 100 bases:

>100_bases
TTGATACAGACCAACTGGTTGATGAAATTGAAGATATGGTTCGTCAGCGTGTTAAAGAAATTGAAGAAGCTAAATCTAAA
GAAATTATTCGTAGTTCATC

Downstream 100 bases:

>100_bases
GCTGAAGAATTGAAGGATAAAGGACACTCACATGAGCGCATTAAGTGAAGAAGAACAACTCGATAACTTGAAGTCGTTCA
CGAAAAAATATGGTTCGGCC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSSIVAIKGFNDVLPTQTAAWRRLEQHLASLMDAYGYQQIRLPIVEQTGLFKRAIGDATDIVEKEMYTFFDKGTPPESLT
LRPEGTAGCVRAMLEHNLLRGATPRVWYVGPMFRYEKPQKGRYRQFHQFGVETFGVATPDIDAELILMTARLWKRMGVSE
KVQLELNTLGEIDERAAYRTALVEFLTQHKEALDEDSQRRLGTNPLRILDSKVESTQKILENAPKLHDFLQEDSLAHFNQ
LQEYLTHAGVSFVINQKLVRGLDYYNKTVFEWTTTALGSQGTVCAGGRYDGLVGQLKGKADQSVPAVGFAMGMERLLLLL
EQVEQAEVVRDCDVFLVAESAFQGHALVLAEQIRDQFEGLASTIRVKTGSQGSMKSQMKKADQSGAHYAVILGEREWTTQ
ELTVKELATSEQSQVAISELVPFLVKKFEK

Sequences:

>Translated_430_residues
MSSIVAIKGFNDVLPTQTAAWRRLEQHLASLMDAYGYQQIRLPIVEQTGLFKRAIGDATDIVEKEMYTFFDKGTPPESLT
LRPEGTAGCVRAMLEHNLLRGATPRVWYVGPMFRYEKPQKGRYRQFHQFGVETFGVATPDIDAELILMTARLWKRMGVSE
KVQLELNTLGEIDERAAYRTALVEFLTQHKEALDEDSQRRLGTNPLRILDSKVESTQKILENAPKLHDFLQEDSLAHFNQ
LQEYLTHAGVSFVINQKLVRGLDYYNKTVFEWTTTALGSQGTVCAGGRYDGLVGQLKGKADQSVPAVGFAMGMERLLLLL
EQVEQAEVVRDCDVFLVAESAFQGHALVLAEQIRDQFEGLASTIRVKTGSQGSMKSQMKKADQSGAHYAVILGEREWTTQ
ELTVKELATSEQSQVAISELVPFLVKKFEK
>Mature_429_residues
SSIVAIKGFNDVLPTQTAAWRRLEQHLASLMDAYGYQQIRLPIVEQTGLFKRAIGDATDIVEKEMYTFFDKGTPPESLTL
RPEGTAGCVRAMLEHNLLRGATPRVWYVGPMFRYEKPQKGRYRQFHQFGVETFGVATPDIDAELILMTARLWKRMGVSEK
VQLELNTLGEIDERAAYRTALVEFLTQHKEALDEDSQRRLGTNPLRILDSKVESTQKILENAPKLHDFLQEDSLAHFNQL
QEYLTHAGVSFVINQKLVRGLDYYNKTVFEWTTTALGSQGTVCAGGRYDGLVGQLKGKADQSVPAVGFAMGMERLLLLLE
QVEQAEVVRDCDVFLVAESAFQGHALVLAEQIRDQFEGLASTIRVKTGSQGSMKSQMKKADQSGAHYAVILGEREWTTQE
LTVKELATSEQSQVAISELVPFLVKKFEK

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=452, Percent_Identity=24.5575221238938, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI15029520, Length=463, Percent_Identity=25.2699784017279, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1788861, Length=419, Percent_Identity=57.0405727923628, Blast_Score=475, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI71993693, Length=464, Percent_Identity=23.4913793103448, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71993686, Length=464, Percent_Identity=23.4913793103448, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325290, Length=462, Percent_Identity=22.7272727272727, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_ACIAD (Q6FEM2)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045308.1
- ProteinModelPortal:   Q6FEM2
- SMR:   Q6FEM2
- STRING:   Q6FEM2
- GeneID:   2878931
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0562
- NMPDR:   fig|62977.3.peg.47
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- PhylomeDB:   Q6FEM2
- ProtClustDB:   PRK00037
- BioCyc:   ASP62977:ACIAD0562-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 48300; Mature: 48169

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIVAIKGFNDVLPTQTAAWRRLEQHLASLMDAYGYQQIRLPIVEQTGLFKRAIGDATD
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCHHHHHHCCHHH
IVEKEMYTFFDKGTPPESLTLRPEGTAGCVRAMLEHNLLRGATPRVWYVGPMFRYEKPQK
HHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCHHHCCCCCC
GRYRQFHQFGVETFGVATPDIDAELILMTARLWKRMGVSEKVQLELNTLGEIDERAAYRT
CHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHCCHHHHHHHHH
ALVEFLTQHKEALDEDSQRRLGTNPLRILDSKVESTQKILENAPKLHDFLQEDSLAHFNQ
HHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHH
LQEYLTHAGVSFVINQKLVRGLDYYNKTVFEWTTTALGSQGTVCAGGRYDGLVGQLKGKA
HHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEECCCCCCCHHHHHCCCC
DQSVPAVGFAMGMERLLLLLEQVEQAEVVRDCDVFLVAESAFQGHALVLAEQIRDQFEGL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHH
ASTIRVKTGSQGSMKSQMKKADQSGAHYAVILGEREWTTQELTVKELATSEQSQVAISEL
HHHEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHH
VPFLVKKFEK
HHHHHHHHCC
>Mature Secondary Structure 
SSIVAIKGFNDVLPTQTAAWRRLEQHLASLMDAYGYQQIRLPIVEQTGLFKRAIGDATD
CCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCHHHHHHCCHHH
IVEKEMYTFFDKGTPPESLTLRPEGTAGCVRAMLEHNLLRGATPRVWYVGPMFRYEKPQK
HHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCHHHCCCCCC
GRYRQFHQFGVETFGVATPDIDAELILMTARLWKRMGVSEKVQLELNTLGEIDERAAYRT
CHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHCCHHHHHHHHH
ALVEFLTQHKEALDEDSQRRLGTNPLRILDSKVESTQKILENAPKLHDFLQEDSLAHFNQ
HHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHH
LQEYLTHAGVSFVINQKLVRGLDYYNKTVFEWTTTALGSQGTVCAGGRYDGLVGQLKGKA
HHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEECCCCCCCHHHHHCCCC
DQSVPAVGFAMGMERLLLLLEQVEQAEVVRDCDVFLVAESAFQGHALVLAEQIRDQFEGL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHH
ASTIRVKTGSQGSMKSQMKKADQSGAHYAVILGEREWTTQELTVKELATSEQSQVAISEL
HHHEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHH
VPFLVKKFEK
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA