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The map label for this gene is ispG

Identifier: 50083797

GI number: 50083797

Start: 552069

End: 553184

Strand: Direct

Name: ispG

Synonym: ACIAD0561

Alternate gene names: 50083797

Gene position: 552069-553184 (Clockwise)

Preceding gene: 50083796

Following gene: 50083798

Centisome position: 15.34

GC content: 43.82

Gene sequence:

>1116_bases
ATGATCGAAAATCCAATTAAACGCCGCCCCACTCGCAAAATTCGTGTTGGCTCTGTTTATGTGGGCGGAGATGCGCCAAT
CAGTATACAAAGCATGACCAATACCGAAACCTGCGATGTCGATGCAACAGTTGCACAGATTCAGCGCTGTGCTGACGCAG
GGGTGGATATCATGCGTGTTTCTGTACCTTCTATGGAAGCTGCTGAGGCATTTGGCAAAATTCGCCAGCGTGTATCTGTG
CCATTGGTGGCGGATATTCACTTTGATCACCGGATTGCACTTGCTGTTGCAGATTATGGCGCAGATTGTCTGCGTATTAA
CCCAGGTAATATTGGTTCTGATCAAAAAGTCCGTGAAGTGGTTGCAGCAGCGCGCCATCATGGAATTTCGATTCGCATTG
GTGTCAATGCGGGATCACTTGAAAAAGATTTGCAGAAAAAATATGGTGAACCGACAGGTGCGGCTCTGTTGGAGTCGGCC
ATGCGTCATATTGATATTTTGGATCGTCTTGATTTTCACGAGTTTAAAGTGAGTGTGAAAGCATCGAATGTGTTTTTGAC
CATGGATGCTTATCGTTTATTATCTCAACAGATTGATAATCCATTGCACTTGGGTGTAACAGAGGCTGGTATTTATCGTA
CGGGTACGGTTAAATCTGCGATTGCACTGGGTGGCTTGCTGATGGAAGGTATAGGCGATACTATGCGTATCTCGCTTGCT
GCTGAACCTGAAGACGAAGTCAAAATTGGTTTTGATATTTTGAAATCACTTGGATTGCGTTCTAACGGGATTAATTTTAT
TGCCTGCCCGAGTTGTTCGCGTCAGGAATTTAATGTCATTAAAGTGATGCAGGCACTCGAAGAGCGTCTTGAAGATATTC
GAACACCGATGGATTTGTCGGTGATTGGTTGTAAAGTAAATGGTCCGGGTGAAGCCAAGGAAGCTGATATCGGTATTGTG
GGGGCTTCTCCTCGTTCTTTGGTGTATCGTAATGGTGAAAAAAGTCACTTGATTGATACAGACCAACTGGTTGATGAAAT
TGAAGATATGGTTCGTCAGCGTGTTAAAGAAATTGAAGAAGCTAAATCTAAAGAAATTATTCGTAGTTCATCATGA

Upstream 100 bases:

>100_bases
TAAGCCTGAATCAAGAGCAGATTTCATTGTCTCCTTATACCGTAAACGGTAAAGCAGAATTCCGTTTATCGCGTTAAGAC
AGAGAAAGTAGAGCAATACG

Downstream 100 bases:

>100_bases
GTTCAATTGTCGCAATAAAAGGTTTTAATGACGTTCTTCCTACTCAAACAGCAGCATGGAGACGTCTTGAGCAACATCTC
GCATCCTTGATGGATGCTTA

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MIENPIKRRPTRKIRVGSVYVGGDAPISIQSMTNTETCDVDATVAQIQRCADAGVDIMRVSVPSMEAAEAFGKIRQRVSV
PLVADIHFDHRIALAVADYGADCLRINPGNIGSDQKVREVVAAARHHGISIRIGVNAGSLEKDLQKKYGEPTGAALLESA
MRHIDILDRLDFHEFKVSVKASNVFLTMDAYRLLSQQIDNPLHLGVTEAGIYRTGTVKSAIALGGLLMEGIGDTMRISLA
AEPEDEVKIGFDILKSLGLRSNGINFIACPSCSRQEFNVIKVMQALEERLEDIRTPMDLSVIGCKVNGPGEAKEADIGIV
GASPRSLVYRNGEKSHLIDTDQLVDEIEDMVRQRVKEIEEAKSKEIIRSSS

Sequences:

>Translated_371_residues
MIENPIKRRPTRKIRVGSVYVGGDAPISIQSMTNTETCDVDATVAQIQRCADAGVDIMRVSVPSMEAAEAFGKIRQRVSV
PLVADIHFDHRIALAVADYGADCLRINPGNIGSDQKVREVVAAARHHGISIRIGVNAGSLEKDLQKKYGEPTGAALLESA
MRHIDILDRLDFHEFKVSVKASNVFLTMDAYRLLSQQIDNPLHLGVTEAGIYRTGTVKSAIALGGLLMEGIGDTMRISLA
AEPEDEVKIGFDILKSLGLRSNGINFIACPSCSRQEFNVIKVMQALEERLEDIRTPMDLSVIGCKVNGPGEAKEADIGIV
GASPRSLVYRNGEKSHLIDTDQLVDEIEDMVRQRVKEIEEAKSKEIIRSSS
>Mature_371_residues
MIENPIKRRPTRKIRVGSVYVGGDAPISIQSMTNTETCDVDATVAQIQRCADAGVDIMRVSVPSMEAAEAFGKIRQRVSV
PLVADIHFDHRIALAVADYGADCLRINPGNIGSDQKVREVVAAARHHGISIRIGVNAGSLEKDLQKKYGEPTGAALLESA
MRHIDILDRLDFHEFKVSVKASNVFLTMDAYRLLSQQIDNPLHLGVTEAGIYRTGTVKSAIALGGLLMEGIGDTMRISLA
AEPEDEVKIGFDILKSLGLRSNGINFIACPSCSRQEFNVIKVMQALEERLEDIRTPMDLSVIGCKVNGPGEAKEADIGIV
GASPRSLVYRNGEKSHLIDTDQLVDEIEDMVRQRVKEIEEAKSKEIIRSSS

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=358, Percent_Identity=63.1284916201117, Blast_Score=473, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_ACIAD (Q6FEM3)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045307.1
- ProteinModelPortal:   Q6FEM3
- SMR:   Q6FEM3
- STRING:   Q6FEM3
- GeneID:   2879846
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0561
- NMPDR:   fig|62977.3.peg.46
- eggNOG:   COG0821
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- PhylomeDB:   Q6FEM3
- ProtClustDB:   PRK00366
- BioCyc:   ASP62977:ACIAD0561-MONOMER
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 40502; Mature: 40502

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIENPIKRRPTRKIRVGSVYVGGDAPISIQSMTNTETCDVDATVAQIQRCADAGVDIMRV
CCCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEE
SVPSMEAAEAFGKIRQRVSVPLVADIHFDHRIALAVADYGADCLRINPGNIGSDQKVREV
ECCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCEEEECCCCCCCHHHHHHH
VAAARHHGISIRIGVNAGSLEKDLQKKYGEPTGAALLESAMRHIDILDRLDFHEFKVSVK
HHHHHHCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHEEEEEE
ASNVFLTMDAYRLLSQQIDNPLHLGVTEAGIYRTGTVKSAIALGGLLMEGIGDTMRISLA
ECEEEEEHHHHHHHHHHCCCCEEECCCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEEEE
AEPEDEVKIGFDILKSLGLRSNGINFIACPSCSRQEFNVIKVMQALEERLEDIRTPMDLS
CCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
VIGCKVNGPGEAKEADIGIVGASPRSLVYRNGEKSHLIDTDQLVDEIEDMVRQRVKEIEE
EEEEEECCCCCCCCCCEEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AKSKEIIRSSS
HHHHHHHCCCC
>Mature Secondary Structure
MIENPIKRRPTRKIRVGSVYVGGDAPISIQSMTNTETCDVDATVAQIQRCADAGVDIMRV
CCCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEE
SVPSMEAAEAFGKIRQRVSVPLVADIHFDHRIALAVADYGADCLRINPGNIGSDQKVREV
ECCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCEEEECCCCCCCHHHHHHH
VAAARHHGISIRIGVNAGSLEKDLQKKYGEPTGAALLESAMRHIDILDRLDFHEFKVSVK
HHHHHHCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHEEEEEE
ASNVFLTMDAYRLLSQQIDNPLHLGVTEAGIYRTGTVKSAIALGGLLMEGIGDTMRISLA
ECEEEEEHHHHHHHHHHCCCCEEECCCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEEEE
AEPEDEVKIGFDILKSLGLRSNGINFIACPSCSRQEFNVIKVMQALEERLEDIRTPMDLS
CCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
VIGCKVNGPGEAKEADIGIVGASPRSLVYRNGEKSHLIDTDQLVDEIEDMVRQRVKEIEE
EEEEEECCCCCCCCCCEEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AKSKEIIRSSS
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA