| Definition | Acinetobacter sp. ADP1 chromosome, complete genome. |
|---|---|
| Accession | NC_005966 |
| Length | 3,598,621 |
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The map label for this gene is leuA [H]
Identifier: 50083768
GI number: 50083768
Start: 514557
End: 516254
Strand: Reverse
Name: leuA [H]
Synonym: ACIAD0530
Alternate gene names: 50083768
Gene position: 516254-514557 (Counterclockwise)
Preceding gene: 50083769
Following gene: 50083767
Centisome position: 14.35
GC content: 41.99
Gene sequence:
>1698_bases ATGATGTTGGCAGATCCAAGCAAAAAATACCGTCGCATGTACCAGCGAGTCGATTTACCAGACCGCCAGTGGCCAAATAA TGAAATTACCAAAGCGCCCATCTGGATGAGTACCGATTTACGTGATGGTAACCAAGCTATTTTTGAACCGATGGATATCG ATCAAAAATTTAAAATGTTTCAAATGTTGGTCAAAATTGGCTTTAAACATATTGAAATTGGTTTTCCATCTGCATCACAG ATTGATTTTGACTTTACCCGCAAATTAATTGAAGAAAACCATATTCCAGATGATGTGTATATCGAAGTATTGGTACAGGC ACGCGATCATTTAATTGAGCGTACTTTTGAATCTTTAGCTGGTGCTAAACGCGCGATCGTGCATATTTATAATGCCAACT CTCCAACTTTCCGCCAGAAAGTTTTAAATGTCGGCGTTGAGGGTGCCAAAAAATTGGCCATCAATGCTGCTCATAAAGTC AAGGAATATGCAGCACAGTATCCGCAAACTGACTGGATTTTTCAGTACAGTCCAGAATGCTTCTCTGCCACTGAATTAGA AGTGGCAAAAGATGTCTGTGACGCTGTGACTGAAATCTGGGAAGCATCACCAGAAAATAAAGTAATCTTAAACTTGCCAG CGACTGTCGAAGTGTCAACACCGAATGTCTATGCCGATCAAATTGAGTGGATGCACCGTAATCTTGCTCGTCGTGATGGC GTGATTATTTCTGTTCACTGCCATAATGACCGTGGCTGTGGGATTGCAGCATCTGAGCTTGCCATTATGGCGGGTGCAGA CCGTGTTGAAGGCTGTGTGTTTGGAAACGGCGAACGTACAGGTAATGTCGACGTTGCTGCAATTGCTTTGAATATGTATA CCCAAGGTGTGGCACCAGAGTTAGATTTTTCTAATATCAATGAAATTATTGCGACTGTTGAAGAATGTACAGGCTTACCT GTACATCCGCGTCATCCATATGCGGGTGATTTGGTCTTCACCGCATTCTCTGGTTCACATCAGGATGCCATTAAGAAAGG CTTTGAATTCCAGAAAAATGAAGACATCTGGGATATGCCGTACTTACCAATCGATCCAAAAGATTTGGGGCGTGACTACG ATGCGGTGATTCGTGTCAACAGCCAATCGGGTAAAGGTGGCATTGCGTACTTGTTAGAATCGAACTACAACGTGGTGTTA CCGCGTCGTTTACAGATTGAGTTTTCACAAATCGTTCAACAAACCACAGATGCCAATGGTACAGAAATCAGTGCCTCTGA AATCTGGAAATTATTCCAAGATACCTATGTGCAAGCAAAAGAACAGCACTATACTGCGAAAAACTATCGTTTATCTGATA TCAATGGAACCCAAATTATTGAATTAGATATTGATATTGATGGTGAAATTCAACAGCTTCGCGGTGAAGGTAATGGACCA ATTTCCGCATTTATGAATGCACTTCAATTACCAATTGATGTATTAAACTATGAAGAACGCAGCATCAGCTCAGGTGCTAA TGCCAAAGCCTTAACACTGATTGAAATTCAAGTAAAAGGTACTGGCAAGAGTGCATTTGGTGCAGGCGTACATGACAATA CCGTCACTTCATCTATTGAAGCGATCCTTGCATGTACCAACCGCTTGATTGAGCAAGGTGTACTCAGCACAGAACAAGTT GCTGCTGCCGCAGTTTAA
Upstream 100 bases:
>100_bases CTTGTTTTTTATTTAAATTTTAGTCATTCAATACCATATATAAATGTAGTGCTTTGCCTGAGAACAATCCCTCAAGCGCG CTACGACAAGGAGCACTCTC
Downstream 100 bases:
>100_bases TCTCGGTAAAAGACTCTTTTTGAAAGGATACCTATGGTATCCTTTCTTTTATTATTGATTTAAAGATAAAAGGCGAATTG CTCCCGTGTCTTTTCCATCT
Product: 2-isopropylmalate synthase
Products: NA
Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]
Number of amino acids: Translated: 565; Mature: 565
Protein sequence:
>565_residues MMLADPSKKYRRMYQRVDLPDRQWPNNEITKAPIWMSTDLRDGNQAIFEPMDIDQKFKMFQMLVKIGFKHIEIGFPSASQ IDFDFTRKLIEENHIPDDVYIEVLVQARDHLIERTFESLAGAKRAIVHIYNANSPTFRQKVLNVGVEGAKKLAINAAHKV KEYAAQYPQTDWIFQYSPECFSATELEVAKDVCDAVTEIWEASPENKVILNLPATVEVSTPNVYADQIEWMHRNLARRDG VIISVHCHNDRGCGIAASELAIMAGADRVEGCVFGNGERTGNVDVAAIALNMYTQGVAPELDFSNINEIIATVEECTGLP VHPRHPYAGDLVFTAFSGSHQDAIKKGFEFQKNEDIWDMPYLPIDPKDLGRDYDAVIRVNSQSGKGGIAYLLESNYNVVL PRRLQIEFSQIVQQTTDANGTEISASEIWKLFQDTYVQAKEQHYTAKNYRLSDINGTQIIELDIDIDGEIQQLRGEGNGP ISAFMNALQLPIDVLNYEERSISSGANAKALTLIEIQVKGTGKSAFGAGVHDNTVTSSIEAILACTNRLIEQGVLSTEQV AAAAV
Sequences:
>Translated_565_residues MMLADPSKKYRRMYQRVDLPDRQWPNNEITKAPIWMSTDLRDGNQAIFEPMDIDQKFKMFQMLVKIGFKHIEIGFPSASQ IDFDFTRKLIEENHIPDDVYIEVLVQARDHLIERTFESLAGAKRAIVHIYNANSPTFRQKVLNVGVEGAKKLAINAAHKV KEYAAQYPQTDWIFQYSPECFSATELEVAKDVCDAVTEIWEASPENKVILNLPATVEVSTPNVYADQIEWMHRNLARRDG VIISVHCHNDRGCGIAASELAIMAGADRVEGCVFGNGERTGNVDVAAIALNMYTQGVAPELDFSNINEIIATVEECTGLP VHPRHPYAGDLVFTAFSGSHQDAIKKGFEFQKNEDIWDMPYLPIDPKDLGRDYDAVIRVNSQSGKGGIAYLLESNYNVVL PRRLQIEFSQIVQQTTDANGTEISASEIWKLFQDTYVQAKEQHYTAKNYRLSDINGTQIIELDIDIDGEIQQLRGEGNGP ISAFMNALQLPIDVLNYEERSISSGANAKALTLIEIQVKGTGKSAFGAGVHDNTVTSSIEAILACTNRLIEQGVLSTEQV AAAAV >Mature_565_residues MMLADPSKKYRRMYQRVDLPDRQWPNNEITKAPIWMSTDLRDGNQAIFEPMDIDQKFKMFQMLVKIGFKHIEIGFPSASQ IDFDFTRKLIEENHIPDDVYIEVLVQARDHLIERTFESLAGAKRAIVHIYNANSPTFRQKVLNVGVEGAKKLAINAAHKV KEYAAQYPQTDWIFQYSPECFSATELEVAKDVCDAVTEIWEASPENKVILNLPATVEVSTPNVYADQIEWMHRNLARRDG VIISVHCHNDRGCGIAASELAIMAGADRVEGCVFGNGERTGNVDVAAIALNMYTQGVAPELDFSNINEIIATVEECTGLP VHPRHPYAGDLVFTAFSGSHQDAIKKGFEFQKNEDIWDMPYLPIDPKDLGRDYDAVIRVNSQSGKGGIAYLLESNYNVVL PRRLQIEFSQIVQQTTDANGTEISASEIWKLFQDTYVQAKEQHYTAKNYRLSDINGTQIIELDIDIDGEIQQLRGEGNGP ISAFMNALQLPIDVLNYEERSISSGANAKALTLIEIQVKGTGKSAFGAGVHDNTVTSSIEAILACTNRLIEQGVLSTEQV AAAAV
Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]
COG id: COG0119
COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]
Homologues:
Organism=Escherichia coli, GI1786261, Length=537, Percent_Identity=23.0912476722533, Blast_Score=105, Evalue=5e-24, Organism=Saccharomyces cerevisiae, GI6324225, Length=580, Percent_Identity=48.9655172413793, Blast_Score=566, Evalue=1e-162, Organism=Saccharomyces cerevisiae, GI6324682, Length=572, Percent_Identity=48.951048951049, Blast_Score=558, Evalue=1e-159,
Paralogues:
None
Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013709 - InterPro: IPR002034 - InterPro: IPR013785 - InterPro: IPR005668 - InterPro: IPR000891 [H]
Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]
EC number: =2.3.3.13 [H]
Molecular weight: Translated: 62949; Mature: 62949
Theoretical pI: Translated: 4.71; Mature: 4.71
Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.2 %Cys (Translated Protein) 2.1 %Met (Translated Protein) 3.4 %Cys+Met (Translated Protein) 1.2 %Cys (Mature Protein) 2.1 %Met (Mature Protein) 3.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MMLADPSKKYRRMYQRVDLPDRQWPNNEITKAPIWMSTDLRDGNQAIFEPMDIDQKFKMF CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHCCCCCCHHHHHHH QMLVKIGFKHIEIGFPSASQIDFDFTRKLIEENHIPDDVYIEVLVQARDHLIERTFESLA HHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC GAKRAIVHIYNANSPTFRQKVLNVGVEGAKKLAINAAHKVKEYAAQYPQTDWIFQYSPEC CCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC FSATELEVAKDVCDAVTEIWEASPENKVILNLPATVEVSTPNVYADQIEWMHRNLARRDG CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHCC VIISVHCHNDRGCGIAASELAIMAGADRVEGCVFGNGERTGNVDVAAIALNMYTQGVAPE EEEEEEECCCCCCCEEHHHHHEEECCCHHCEEEECCCCCCCCEEEEEEEEEHHHCCCCCC LDFSNINEIIATVEECTGLPVHPRHPYAGDLVFTAFSGSHQDAIKKGFEFQKNEDIWDMP CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCC YLPIDPKDLGRDYDAVIRVNSQSGKGGIAYLLESNYNVVLPRRLQIEFSQIVQQTTDANG CCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCEEECEEEEHHHHHHHHHHCCCCC TEISASEIWKLFQDTYVQAKEQHYTAKNYRLSDINGTQIIELDIDIDGEIQQLRGEGNGP CEECHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEECCCCHHHHHCCCCCCC ISAFMNALQLPIDVLNYEERSISSGANAKALTLIEIQVKGTGKSAFGAGVHDNTVTSSIE HHHHHHHHHCCHHHHCCCHHHHCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHH AILACTNRLIEQGVLSTEQVAAAAV HHHHHHHHHHHHCCCCHHHHHHHCC >Mature Secondary Structure MMLADPSKKYRRMYQRVDLPDRQWPNNEITKAPIWMSTDLRDGNQAIFEPMDIDQKFKMF CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHCCCCCCHHHHHHH QMLVKIGFKHIEIGFPSASQIDFDFTRKLIEENHIPDDVYIEVLVQARDHLIERTFESLA HHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC GAKRAIVHIYNANSPTFRQKVLNVGVEGAKKLAINAAHKVKEYAAQYPQTDWIFQYSPEC CCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC FSATELEVAKDVCDAVTEIWEASPENKVILNLPATVEVSTPNVYADQIEWMHRNLARRDG CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHCC VIISVHCHNDRGCGIAASELAIMAGADRVEGCVFGNGERTGNVDVAAIALNMYTQGVAPE EEEEEEECCCCCCCEEHHHHHEEECCCHHCEEEECCCCCCCCEEEEEEEEEHHHCCCCCC LDFSNINEIIATVEECTGLPVHPRHPYAGDLVFTAFSGSHQDAIKKGFEFQKNEDIWDMP CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCC YLPIDPKDLGRDYDAVIRVNSQSGKGGIAYLLESNYNVVLPRRLQIEFSQIVQQTTDANG CCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCEEECEEEEHHHHHHHHHHCCCCC TEISASEIWKLFQDTYVQAKEQHYTAKNYRLSDINGTQIIELDIDIDGEIQQLRGEGNGP CEECHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEECCCCHHHHHCCCCCCC ISAFMNALQLPIDVLNYEERSISSGANAKALTLIEIQVKGTGKSAFGAGVHDNTVTSSIE HHHHHHHHHCCHHHHCCCHHHHCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHH AILACTNRLIEQGVLSTEQVAAAAV HHHHHHHHHHHHCCCCHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA