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The map label for this gene is thyA

Identifier: 50083753

GI number: 50083753

Start: 502907

End: 503749

Strand: Reverse

Name: thyA

Synonym: ACIAD0515

Alternate gene names: 50083753

Gene position: 503749-502907 (Counterclockwise)

Preceding gene: 50083754

Following gene: 50083752

Centisome position: 14.0

GC content: 44.13

Gene sequence:

>843_bases
ATGCGCACCTATTTAGACCTTTTACAACATATCCTCGACAACGGCGGCGACAAAGGTGACCGTACAGGCACAGGGACACG
TTCTGTATTTGGTCATCAAATGCGTTTTGACCTATCTCAAGGGTTTCCTTTACTTACCACCAAGAAAGTACATTTCCGCT
CAATTGTGATTGAGCTGCTGTGGTTTCTCAAAGGCGATACCAACGTTCAATATTTAAAAGACCATAAAGTCAGCATTTGG
GATGAGTGGGCAACGGCAGAGCAAACTGCACGTTTTGGCCGTCCAGAAGGCGAGCTTGGCCCAGTTTATGGACACCAATG
GCGTAATTTCGGTGCTACTCAAAATTCAGATGGGAGCTACAAAAACGACGGTTTTGATCAAATCAAATGGCTCATTAATG
AAATCAAGACCAATCCGAATTCACGTCGCTTGATTGTATCAGGCTGGAATCCAAATGAAGCCGGACAAGTGGCTTTACCA
CCTTGTCATACCCTGTTTCAGTTTTTTGTTCAGGATGGCAAACTCTCTTGTCAGCTCTATCAACGTAGTGCCGATGTGTT
TTTAGGTGTGCCATTTAACATTGCCAGTTATGCCCTTCTCACCCATATGATTGCGCAGGTGTGTGGCCTAGGTGTAGGCG
ATTTTGTCTGGACGGGCGGTGACACCCATTTATACGCCAATCATTTTGAGCAAGCACAACTTCAACTGACACGTGAACCG
TTACCACTTTGTCAGCTTAAATTAAACCCTGAAATTACCGATCTGTTTGATTTTAAATTTGAAGATATTGAAATTGTGGA
TTATCAATCCCACCCTGCAATCAAAGCCCCTGTTGCCGTATAA

Upstream 100 bases:

>100_bases
ATTGTTTACTGTTCTTTGAAGGCAAAAACCGCTAAAATTTGCAGTCGATTGAAACTTGTTGTTGCTTAGTCAAGCGGCAA
CTATACGATTAGAGAGAATC

Downstream 100 bases:

>100_bases
GGAAGCTCAGCAATGGCATTTCAAGATTTAGAAGTCGTGCATGTGGTTGCGATGGATCAAAAACAATGTATTGGTAAAGG
CAATGCATTACCTTGGCACA

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MRTYLDLLQHILDNGGDKGDRTGTGTRSVFGHQMRFDLSQGFPLLTTKKVHFRSIVIELLWFLKGDTNVQYLKDHKVSIW
DEWATAEQTARFGRPEGELGPVYGHQWRNFGATQNSDGSYKNDGFDQIKWLINEIKTNPNSRRLIVSGWNPNEAGQVALP
PCHTLFQFFVQDGKLSCQLYQRSADVFLGVPFNIASYALLTHMIAQVCGLGVGDFVWTGGDTHLYANHFEQAQLQLTREP
LPLCQLKLNPEITDLFDFKFEDIEIVDYQSHPAIKAPVAV

Sequences:

>Translated_280_residues
MRTYLDLLQHILDNGGDKGDRTGTGTRSVFGHQMRFDLSQGFPLLTTKKVHFRSIVIELLWFLKGDTNVQYLKDHKVSIW
DEWATAEQTARFGRPEGELGPVYGHQWRNFGATQNSDGSYKNDGFDQIKWLINEIKTNPNSRRLIVSGWNPNEAGQVALP
PCHTLFQFFVQDGKLSCQLYQRSADVFLGVPFNIASYALLTHMIAQVCGLGVGDFVWTGGDTHLYANHFEQAQLQLTREP
LPLCQLKLNPEITDLFDFKFEDIEIVDYQSHPAIKAPVAV
>Mature_280_residues
MRTYLDLLQHILDNGGDKGDRTGTGTRSVFGHQMRFDLSQGFPLLTTKKVHFRSIVIELLWFLKGDTNVQYLKDHKVSIW
DEWATAEQTARFGRPEGELGPVYGHQWRNFGATQNSDGSYKNDGFDQIKWLINEIKTNPNSRRLIVSGWNPNEAGQVALP
PCHTLFQFFVQDGKLSCQLYQRSADVFLGVPFNIASYALLTHMIAQVCGLGVGDFVWTGGDTHLYANHFEQAQLQLTREP
LPLCQLKLNPEITDLFDFKFEDIEIVDYQSHPAIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=281, Percent_Identity=51.9572953736655, Blast_Score=292, Evalue=2e-79,
Organism=Escherichia coli, GI1789191, Length=280, Percent_Identity=59.6428571428571, Blast_Score=347, Evalue=6e-97,
Organism=Caenorhabditis elegans, GI71993377, Length=282, Percent_Identity=47.5177304964539, Blast_Score=273, Evalue=7e-74,
Organism=Saccharomyces cerevisiae, GI6324648, Length=290, Percent_Identity=40.6896551724138, Blast_Score=208, Evalue=1e-54,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=54.416961130742, Blast_Score=309, Evalue=1e-84,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_ACIAD (Q6FER7)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045263.1
- ProteinModelPortal:   Q6FER7
- SMR:   Q6FER7
- STRING:   Q6FER7
- GeneID:   2880752
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0515
- NMPDR:   fig|62977.3.peg.1278
- eggNOG:   COG0207
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- PhylomeDB:   Q6FER7
- ProtClustDB:   PRK01827
- BioCyc:   ASP62977:ACIAD0515-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 31774; Mature: 31774

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTYLDLLQHILDNGGDKGDRTGTGTRSVFGHQMRFDLSQGFPLLTTKKVHFRSIVIELL
CCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEEECCCCCCEEEECHHHHHHHHHHHH
WFLKGDTNVQYLKDHKVSIWDEWATAEQTARFGRPEGELGPVYGHQWRNFGATQNSDGSY
HHHCCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCC
KNDGFDQIKWLINEIKTNPNSRRLIVSGWNPNEAGQVALPPCHTLFQFFVQDGKLSCQLY
CCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCHHHHHHHHHHCCCEEEEEE
QRSADVFLGVPFNIASYALLTHMIAQVCGLGVGDFVWTGGDTHLYANHFEQAQLQLTREP
ECCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEHHHHHHHHHHHHCCC
LPLCQLKLNPEITDLFDFKFEDIEIVDYQSHPAIKAPVAV
CCEEEEECCCCHHHHHCCCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MRTYLDLLQHILDNGGDKGDRTGTGTRSVFGHQMRFDLSQGFPLLTTKKVHFRSIVIELL
CCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEEECCCCCCEEEECHHHHHHHHHHHH
WFLKGDTNVQYLKDHKVSIWDEWATAEQTARFGRPEGELGPVYGHQWRNFGATQNSDGSY
HHHCCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCC
KNDGFDQIKWLINEIKTNPNSRRLIVSGWNPNEAGQVALPPCHTLFQFFVQDGKLSCQLY
CCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCHHHHHHHHHHCCCEEEEEE
QRSADVFLGVPFNIASYALLTHMIAQVCGLGVGDFVWTGGDTHLYANHFEQAQLQLTREP
ECCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEHHHHHHHHHHHHCCC
LPLCQLKLNPEITDLFDFKFEDIEIVDYQSHPAIKAPVAV
CCEEEEECCCCHHHHHCCCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA