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The map label for this gene is folA [H]

Identifier: 50083752

GI number: 50083752

Start: 502385

End: 502894

Strand: Reverse

Name: folA [H]

Synonym: ACIAD0514

Alternate gene names: 50083752

Gene position: 502894-502385 (Counterclockwise)

Preceding gene: 50083753

Following gene: 50083751

Centisome position: 13.97

GC content: 40.78

Gene sequence:

>510_bases
ATGGCATTTCAAGATTTAGAAGTCGTGCATGTGGTTGCGATGGATCAAAAACAATGTATTGGTAAAGGCAATGCATTACC
TTGGCACATTCCTGCCGATCTCAAACATTTTAAGGAAATCACTCAAGATGGCGTCGTGATTATGGGTCGTAAGACACTTG
AATCCATGGGGCGCACGCTCCCTAAACGTGTCAACTGGGTGATTACCCGTGATCCTAATTGGCAGTTTGAAGGCGCCAAA
GTTGCATCCAGCATAGAAGCTGCACTTAAAGGCGCAGCTCAGGATCTACAACATACTGATAAGAAAACACTGTTTATTAT
TGGTGGTGGGGAAATTTTTAAACAAACCATCAATATTGCAGACCGTTTGGAATTGACGCATGTCGAATTAGATGTACAAG
GTGATGCCTATTATCCTGCGATTCCTTCACAATTTAATAAAGTGTCGAGTGAAAAACACTTTGATGACAAAACAGGTATT
GCGTTTGAGTTCGTAAGTTATCAAAAATAA

Upstream 100 bases:

>100_bases
TACCGATCTGTTTGATTTTAAATTTGAAGATATTGAAATTGTGGATTATCAATCCCACCCTGCAATCAAAGCCCCTGTTG
CCGTATAAGGAAGCTCAGCA

Downstream 100 bases:

>100_bases
AGACAATCTCTAGCTGATTGTCTGCGATGCATAATATTGGAAAAAAAGAATTTTTACATGCCCTAGGGACGGGGCTTCTC
TCTATGATCTTTAGTCTTTT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MAFQDLEVVHVVAMDQKQCIGKGNALPWHIPADLKHFKEITQDGVVIMGRKTLESMGRTLPKRVNWVITRDPNWQFEGAK
VASSIEAALKGAAQDLQHTDKKTLFIIGGGEIFKQTINIADRLELTHVELDVQGDAYYPAIPSQFNKVSSEKHFDDKTGI
AFEFVSYQK

Sequences:

>Translated_169_residues
MAFQDLEVVHVVAMDQKQCIGKGNALPWHIPADLKHFKEITQDGVVIMGRKTLESMGRTLPKRVNWVITRDPNWQFEGAK
VASSIEAALKGAAQDLQHTDKKTLFIIGGGEIFKQTINIADRLELTHVELDVQGDAYYPAIPSQFNKVSSEKHFDDKTGI
AFEFVSYQK
>Mature_168_residues
AFQDLEVVHVVAMDQKQCIGKGNALPWHIPADLKHFKEITQDGVVIMGRKTLESMGRTLPKRVNWVITRDPNWQFEGAKV
ASSIEAALKGAAQDLQHTDKKTLFIIGGGEIFKQTINIADRLELTHVELDVQGDAYYPAIPSQFNKVSSEKHFDDKTGIA
FEFVSYQK

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=178, Percent_Identity=28.6516853932584, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1786233, Length=144, Percent_Identity=35.4166666666667, Blast_Score=92, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17505899, Length=178, Percent_Identity=30.3370786516854, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324810, Length=143, Percent_Identity=32.8671328671329, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18984; Mature: 18853

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFQDLEVVHVVAMDQKQCIGKGNALPWHIPADLKHFKEITQDGVVIMGRKTLESMGRTL
CCCCCCEEEEEEECCHHHHCCCCCCCCEECCHHHHHHHHHHCCCEEEEEHHHHHHHHHHH
PKRVNWVITRDPNWQFEGAKVASSIEAALKGAAQDLQHTDKKTLFIIGGGEIFKQTINIA
HHHCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHH
DRLELTHVELDVQGDAYYPAIPSQFNKVSSEKHFDDKTGIAFEFVSYQK
HHEEEEEEEEEECCCEECCCCCHHHHHHCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure 
AFQDLEVVHVVAMDQKQCIGKGNALPWHIPADLKHFKEITQDGVVIMGRKTLESMGRTL
CCCCCEEEEEEECCHHHHCCCCCCCCEECCHHHHHHHHHHCCCEEEEEHHHHHHHHHHH
PKRVNWVITRDPNWQFEGAKVASSIEAALKGAAQDLQHTDKKTLFIIGGGEIFKQTINIA
HHHCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHH
DRLELTHVELDVQGDAYYPAIPSQFNKVSSEKHFDDKTGIAFEFVSYQK
HHEEEEEEEEEECCCEECCCCCHHHHHHCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]