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The map label for this gene is htrB [H]

Identifier: 50083725

GI number: 50083725

Start: 477033

End: 477968

Strand: Reverse

Name: htrB [H]

Synonym: ACIAD0484

Alternate gene names: 50083725

Gene position: 477968-477033 (Counterclockwise)

Preceding gene: 50083726

Following gene: 50083724

Centisome position: 13.28

GC content: 41.67

Gene sequence:

>936_bases
ATGAGCCAGCAGCAAGCCTATATTCCCAGTAAATTTCAATGGTCTTTTTTATTGCCCAAATATTGGGGTATCTGGATTGG
TATTACATTATTAATGTTACTGGCTATTTTACCATGGGGAATCCAATGGCGTTTGGCTCACGGGTTAGCGAACCTAGCTT
GGAAATATTTAAAATCTAGGCGTAAGACCACTATTCGTAATCTAGAGGTCTGTTTTCCAGAATGGTCACCTGAGGAAGTC
GAGAAACAGGCCAAACGGGTGTTTATCGATATGATGCTCGGTATTTTTGAAACCTTAAATGCCTGGTATTGCCCACAATG
GTTTAAACATCGCTGTACCATTGAAGGCTTAGAACACATCACCAATGCACAAAAACAAGGTCAAGGTGTATTACTACTTG
GTACGCATAGCACTTTACTTGATGCAGGCGGGTATCTTTGTGCACAATACTTCGAGCCAGATGTGGTTTATCGACCTCAA
AACAATCCTCTACTGGATATGCTCATTTATCGTTGCCGTGCAACTATCTATAAACATCAAATTGATCATGATGATATGCG
TGGTTTGATTCGTAAACTCAAGGATGGAGATGCGATCTGGTACAGTCCTGATCAAGATTTTGGTCTTAAACAAGGCGTCA
TGGCACCTTTTTTTGGTGTGCCTGCCGCTACAGTAACTGCACATCGTCGCCTGTTAAAAATTTCTAAAGCAGCAGCAGTG
CCATTGTATTTTTATCGTCACGGCGATGTCCGTGATCCAAAATATCATGTTTTAATTGAAGCAGCAGTTGAAAATATGCC
AAGCGAGGACGAAGTCGATGATGCAACACGTGTTAACCAAATCATCGAAAAGCAACTCCGAATCGCACCCACTCAATATA
TGTGGTTTCATCGCCGTTTTAAGACACGTCCTGAGGGGTATGAAGAGATCTATTAA

Upstream 100 bases:

>100_bases
GACAATTTTGAATACATCCAACTCTCAACTGTCAGTTAAAAATCACTTCATGTTACACTGCTCTATTATTAAATGATTAT
TGGTCACCCCCTAATTCAGC

Downstream 100 bases:

>100_bases
GATAAAGCTATAATGATTTTAAAGTGGTCGTAAGCACAGCCTCAGTTTAAAGTGAAATTCAAATACTTTCGAATAGGCCA
ACCTTACTTTTCAGGGATAA

Product: lipid A biosynthesis lauroyl acyltransferase (heat shock protein B)

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MSQQQAYIPSKFQWSFLLPKYWGIWIGITLLMLLAILPWGIQWRLAHGLANLAWKYLKSRRKTTIRNLEVCFPEWSPEEV
EKQAKRVFIDMMLGIFETLNAWYCPQWFKHRCTIEGLEHITNAQKQGQGVLLLGTHSTLLDAGGYLCAQYFEPDVVYRPQ
NNPLLDMLIYRCRATIYKHQIDHDDMRGLIRKLKDGDAIWYSPDQDFGLKQGVMAPFFGVPAATVTAHRRLLKISKAAAV
PLYFYRHGDVRDPKYHVLIEAAVENMPSEDEVDDATRVNQIIEKQLRIAPTQYMWFHRRFKTRPEGYEEIY

Sequences:

>Translated_311_residues
MSQQQAYIPSKFQWSFLLPKYWGIWIGITLLMLLAILPWGIQWRLAHGLANLAWKYLKSRRKTTIRNLEVCFPEWSPEEV
EKQAKRVFIDMMLGIFETLNAWYCPQWFKHRCTIEGLEHITNAQKQGQGVLLLGTHSTLLDAGGYLCAQYFEPDVVYRPQ
NNPLLDMLIYRCRATIYKHQIDHDDMRGLIRKLKDGDAIWYSPDQDFGLKQGVMAPFFGVPAATVTAHRRLLKISKAAAV
PLYFYRHGDVRDPKYHVLIEAAVENMPSEDEVDDATRVNQIIEKQLRIAPTQYMWFHRRFKTRPEGYEEIY
>Mature_310_residues
SQQQAYIPSKFQWSFLLPKYWGIWIGITLLMLLAILPWGIQWRLAHGLANLAWKYLKSRRKTTIRNLEVCFPEWSPEEVE
KQAKRVFIDMMLGIFETLNAWYCPQWFKHRCTIEGLEHITNAQKQGQGVLLLGTHSTLLDAGGYLCAQYFEPDVVYRPQN
NPLLDMLIYRCRATIYKHQIDHDDMRGLIRKLKDGDAIWYSPDQDFGLKQGVMAPFFGVPAATVTAHRRLLKISKAAAVP
LYFYRHGDVRDPKYHVLIEAAVENMPSEDEVDDATRVNQIIEKQLRIAPTQYMWFHRRFKTRPEGYEEIY

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=304, Percent_Identity=39.4736842105263, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI87082094, Length=307, Percent_Identity=36.8078175895765, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1788161, Length=312, Percent_Identity=29.1666666666667, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 36455; Mature: 36323

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQQAYIPSKFQWSFLLPKYWGIWIGITLLMLLAILPWGIQWRLAHGLANLAWKYLKSR
CCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RKTTIRNLEVCFPEWSPEEVEKQAKRVFIDMMLGIFETLNAWYCPQWFKHRCTIEGLEHI
HHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCHHHHHHHH
TNAQKQGQGVLLLGTHSTLLDAGGYLCAQYFEPDVVYRPQNNPLLDMLIYRCRATIYKHQ
HHHHHCCCCEEEEECCHHHHHCCCHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHC
IDHDDMRGLIRKLKDGDAIWYSPDQDFGLKQGVMAPFFGVPAATVTAHRRLLKISKAAAV
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHCC
PLYFYRHGDVRDPKYHVLIEAAVENMPSEDEVDDATRVNQIIEKQLRIAPTQYMWFHRRF
CEEEEECCCCCCCHHHEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KTRPEGYEEIY
CCCCCCHHCCC
>Mature Secondary Structure 
SQQQAYIPSKFQWSFLLPKYWGIWIGITLLMLLAILPWGIQWRLAHGLANLAWKYLKSR
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RKTTIRNLEVCFPEWSPEEVEKQAKRVFIDMMLGIFETLNAWYCPQWFKHRCTIEGLEHI
HHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCHHHHHHHH
TNAQKQGQGVLLLGTHSTLLDAGGYLCAQYFEPDVVYRPQNNPLLDMLIYRCRATIYKHQ
HHHHHCCCCEEEEECCHHHHHCCCHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHC
IDHDDMRGLIRKLKDGDAIWYSPDQDFGLKQGVMAPFFGVPAATVTAHRRLLKISKAAAV
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHCC
PLYFYRHGDVRDPKYHVLIEAAVENMPSEDEVDDATRVNQIIEKQLRIAPTQYMWFHRRF
CEEEEECCCCCCCHHHEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KTRPEGYEEIY
CCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]