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The map label for this gene is leuD

Identifier: 50083712

GI number: 50083712

Start: 463156

End: 463806

Strand: Direct

Name: leuD

Synonym: ACIAD0466

Alternate gene names: 50083712

Gene position: 463156-463806 (Clockwise)

Preceding gene: 50083711

Following gene: 50083713

Centisome position: 12.87

GC content: 38.1

Gene sequence:

>651_bases
ATGAAAGCATATACAGTAGAACAAGGTATTGTGGCGCCATTAGATCGTGCCAATGTCGATACCGATTTAATTATCCCGAA
ACAGTTTTTGAAATCCATTAAACGCACGGGTTTTGGCGATAATTTGTTTGATGAATTACGTTATTTGGATGAGGGTTATC
CTGGTCAAGATAACGCTAAACGTCCTAAAAACCCTGATTTTATTTTAAATCAGCCATGTTATGCATCTGCGACAGTATTA
ATCGCACGTAAAAACTTTGGTTGTGGTTCAAGCCGAGAACATGCGCCTTGGGCATTAAATGAATATGGTTTTCGTACCGT
GATTGCACCAAGTTTTGCAGATATTTTCTTTAACAACTGCTTTAAAAATGGCATGTTGCCAGTCATCTTGGATGAAGAGA
CTGTTGATCAGTTGTTTAAAGAATGTGCCGCAAGTGAAGATTATCAGTTAACGATTGATCTTGCTGCTCAAGAAGTTAAA
ACACCAACGGGTGAAGCATTCAAATTTGAGATTGATCCATTTCGTAAACATTGCTTGTTAAATGGCCTGGATGATATCGG
TTTGACTTTGCAAAATGCGGGTGCTATCCGAGAATTTGAAAGTAAAGCTAAACAATCACGTCCTTGGGTGTTTCAAGATT
TAAAAGCGTAA

Upstream 100 bases:

>100_bases
TGATTCTGCTTTTTTAGAATGGATTAACGATGCTGAACAAGACACTTTAATTGTGCCTCGTTTAGAGTGTGGTCAAGTTT
AGGCATAAAGAGTTTTTATT

Downstream 100 bases:

>100_bases
TCGACACGGGAGATTTTAAAGATAGCCATAGCGTAGATGCCTAACTCTTGTTAACATGCATGAGCATAAATTGCTGAAAA
CTTTGCTCGAAAAAGGGTTG

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MKAYTVEQGIVAPLDRANVDTDLIIPKQFLKSIKRTGFGDNLFDELRYLDEGYPGQDNAKRPKNPDFILNQPCYASATVL
IARKNFGCGSSREHAPWALNEYGFRTVIAPSFADIFFNNCFKNGMLPVILDEETVDQLFKECAASEDYQLTIDLAAQEVK
TPTGEAFKFEIDPFRKHCLLNGLDDIGLTLQNAGAIREFESKAKQSRPWVFQDLKA

Sequences:

>Translated_216_residues
MKAYTVEQGIVAPLDRANVDTDLIIPKQFLKSIKRTGFGDNLFDELRYLDEGYPGQDNAKRPKNPDFILNQPCYASATVL
IARKNFGCGSSREHAPWALNEYGFRTVIAPSFADIFFNNCFKNGMLPVILDEETVDQLFKECAASEDYQLTIDLAAQEVK
TPTGEAFKFEIDPFRKHCLLNGLDDIGLTLQNAGAIREFESKAKQSRPWVFQDLKA
>Mature_216_residues
MKAYTVEQGIVAPLDRANVDTDLIIPKQFLKSIKRTGFGDNLFDELRYLDEGYPGQDNAKRPKNPDFILNQPCYASATVL
IARKNFGCGSSREHAPWALNEYGFRTVIAPSFADIFFNNCFKNGMLPVILDEETVDQLFKECAASEDYQLTIDLAAQEVK
TPTGEAFKFEIDPFRKHCLLNGLDDIGLTLQNAGAIREFESKAKQSRPWVFQDLKA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=200, Percent_Identity=56, Blast_Score=226, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6321429, Length=205, Percent_Identity=48.780487804878, Blast_Score=197, Evalue=8e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_ACIAD (Q6FEV8)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045222.1
- ProteinModelPortal:   Q6FEV8
- STRING:   Q6FEV8
- GeneID:   2880115
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0466
- NMPDR:   fig|62977.3.peg.1120
- eggNOG:   COG0066
- HOGENOM:   HBG304838
- OMA:   DEISITM
- PhylomeDB:   Q6FEV8
- ProtClustDB:   PRK01641
- BioCyc:   ASP62977:ACIAD0466-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 24339; Mature: 24339

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAYTVEQGIVAPLDRANVDTDLIIPKQFLKSIKRTGFGDNLFDELRYLDEGYPGQDNAK
CCCEECCCCCCCCCCCCCCCCCEECHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCC
RPKNPDFILNQPCYASATVLIARKNFGCGSSREHAPWALNEYGFRTVIAPSFADIFFNNC
CCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCEECCCCCCEEEECHHHHHHHHHHH
FKNGMLPVILDEETVDQLFKECAASEDYQLTIDLAAQEVKTPTGEAFKFEIDPFRKHCLL
HHCCCEEEEECHHHHHHHHHHHHCCCCCEEEEEECHHHHCCCCCCEEEEECCHHHHHHHH
NGLDDIGLTLQNAGAIREFESKAKQSRPWVFQDLKA
CCCHHHCEEECCCCHHHHHHHHHHHCCCCHHHCCCC
>Mature Secondary Structure
MKAYTVEQGIVAPLDRANVDTDLIIPKQFLKSIKRTGFGDNLFDELRYLDEGYPGQDNAK
CCCEECCCCCCCCCCCCCCCCCEECHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCC
RPKNPDFILNQPCYASATVLIARKNFGCGSSREHAPWALNEYGFRTVIAPSFADIFFNNC
CCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCEECCCCCCEEEECHHHHHHHHHHH
FKNGMLPVILDEETVDQLFKECAASEDYQLTIDLAAQEVKTPTGEAFKFEIDPFRKHCLL
HHCCCEEEEECHHHHHHHHHHHHCCCCCEEEEEECHHHHCCCCCCEEEEECCHHHHHHHH
NGLDDIGLTLQNAGAIREFESKAKQSRPWVFQDLKA
CCCHHHCEEECCCCHHHHHHHHHHHCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA