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The map label for this gene is butA [H]

Identifier: 50083686

GI number: 50083686

Start: 430989

End: 431744

Strand: Reverse

Name: butA [H]

Synonym: ACIAD0433

Alternate gene names: 50083686

Gene position: 431744-430989 (Counterclockwise)

Preceding gene: 50083688

Following gene: 50083684

Centisome position: 12.0

GC content: 42.06

Gene sequence:

>756_bases
ATGACTGCTCAAACACTTAAAAATAAAGTGGTTTTAATTACTGGTGGTGCAAAAAATCTCGGTGGATTAATTTCAAGAAA
ATTTGCTGAGCAAGGTGCAAAACTGGCAATTCATTACAACAGTGAATCCACTAAAGCCGACGCTGAAAAAACACTGGCTG
ATGTGCAAGCACTAGGTGCAGATGCCATATTATTACAAGGTGATCTGAGTTCAATTGATCGTATTGAAGCGCTATTTGTC
AAAACCAAAGCACATTTTGGTGGTATCGATATTGCCATCAATACTGTGGGTCGAGTTCTTAAAAAACCGTTTCTGGAAAC
CACCGAAACTGAATTTGATTCAATGAGTGATATCAACTCTAAAATTGCTTACTTCTTTATCCAGTCGGCAGGTCGTCATC
TGAATGATGGCGGTAAAATCTGTACTATTGTCACTTCATTATTGGCAGCTTATACGGGTCTTTACTCGACTTATGAAGGT
TTAAAAGCACCTGTGGAACACTACACCCGCGCCGCCTCTAAAGAGTTTGGTGAGCGTGGTATTTCTGTGACTGCTGTTGC
ACCAGGCCCAATGGATACGCCATTTTTCTATGGTCAGGAAGCACCAGAAGCTGTGGCTTATCACAAATCGGCTTCTGCCC
TTGGCGGCCTCACCAAAATTGAAGATATCGAACCTTTGGTACGTTTCTTGGTTACTGAAGGCTGGTGGATTACAGGTCAA
ACCATTTTTGCGAATGGGGGTTATACCACACGTTAA

Upstream 100 bases:

>100_bases
AAACCAAATTAGCACAATTGATCATTACATGATTGAAATTTAGACTGAATTTCAATCAAATTAATTGTGATTATGGTTTT
ACAGCCAAAAGGGAAACAAC

Downstream 100 bases:

>100_bases
TGATTCGACTTGGCTAGGGAAGCCCTATTCCCTAGCCATTTTTATTGAAGACTACATCGTGATTTTACTGAGATATGTTC
AATCACATGGACCGATTTTA

Product: short chain dehydrogenase

Products: NA

Alternate protein names: Acetoin(diacetyl) reductase; AR; Meso-2,3-butanediol dehydrogenase [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MTAQTLKNKVVLITGGAKNLGGLISRKFAEQGAKLAIHYNSESTKADAEKTLADVQALGADAILLQGDLSSIDRIEALFV
KTKAHFGGIDIAINTVGRVLKKPFLETTETEFDSMSDINSKIAYFFIQSAGRHLNDGGKICTIVTSLLAAYTGLYSTYEG
LKAPVEHYTRAASKEFGERGISVTAVAPGPMDTPFFYGQEAPEAVAYHKSASALGGLTKIEDIEPLVRFLVTEGWWITGQ
TIFANGGYTTR

Sequences:

>Translated_251_residues
MTAQTLKNKVVLITGGAKNLGGLISRKFAEQGAKLAIHYNSESTKADAEKTLADVQALGADAILLQGDLSSIDRIEALFV
KTKAHFGGIDIAINTVGRVLKKPFLETTETEFDSMSDINSKIAYFFIQSAGRHLNDGGKICTIVTSLLAAYTGLYSTYEG
LKAPVEHYTRAASKEFGERGISVTAVAPGPMDTPFFYGQEAPEAVAYHKSASALGGLTKIEDIEPLVRFLVTEGWWITGQ
TIFANGGYTTR
>Mature_250_residues
TAQTLKNKVVLITGGAKNLGGLISRKFAEQGAKLAIHYNSESTKADAEKTLADVQALGADAILLQGDLSSIDRIEALFVK
TKAHFGGIDIAINTVGRVLKKPFLETTETEFDSMSDINSKIAYFFIQSAGRHLNDGGKICTIVTSLLAAYTGLYSTYEGL
KAPVEHYTRAASKEFGERGISVTAVAPGPMDTPFFYGQEAPEAVAYHKSASALGGLTKIEDIEPLVRFLVTEGWWITGQT
IFANGGYTTR

Specific function: Catalyzes the irreversible reduction of 2,3-butanediol to (S)-acetoin in the presence of NADH [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=261, Percent_Identity=26.4367816091954, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI5031737, Length=255, Percent_Identity=26.2745098039216, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1789208, Length=258, Percent_Identity=29.8449612403101, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787335, Length=249, Percent_Identity=25.3012048192771, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1789378, Length=191, Percent_Identity=31.413612565445, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI2367365, Length=187, Percent_Identity=31.5508021390374, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1788459, Length=246, Percent_Identity=26.8292682926829, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790717, Length=191, Percent_Identity=25.130890052356, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787905, Length=247, Percent_Identity=26.7206477732794, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082160, Length=255, Percent_Identity=24.7058823529412, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17560220, Length=265, Percent_Identity=27.9245283018868, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17544670, Length=267, Percent_Identity=27.7153558052434, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17560150, Length=266, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17562904, Length=264, Percent_Identity=24.2424242424242, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17562910, Length=261, Percent_Identity=26.4367816091954, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562990, Length=265, Percent_Identity=26.0377358490566, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17538480, Length=256, Percent_Identity=25.78125, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI193204405, Length=257, Percent_Identity=24.124513618677, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17555706, Length=248, Percent_Identity=25, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17563726, Length=261, Percent_Identity=26.0536398467433, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17551412, Length=256, Percent_Identity=25.390625, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI21355319, Length=262, Percent_Identity=29.0076335877863, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24639444, Length=257, Percent_Identity=27.6264591439689, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24643142, Length=184, Percent_Identity=27.7173913043478, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28571526, Length=253, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24644339, Length=249, Percent_Identity=26.5060240963855, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI21357041, Length=253, Percent_Identity=26.8774703557312, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014007
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.1.1.304 [H]

Molecular weight: Translated: 26991; Mature: 26860

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQTLKNKVVLITGGAKNLGGLISRKFAEQGAKLAIHYNSESTKADAEKTLADVQALGA
CCCHHHCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCC
DAILLQGDLSSIDRIEALFVKTKAHFGGIDIAINTVGRVLKKPFLETTETEFDSMSDINS
CEEEEECCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
KIAYFFIQSAGRHLNDGGKICTIVTSLLAAYTGLYSTYEGLKAPVEHYTRAASKEFGERG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ISVTAVAPGPMDTPFFYGQEAPEAVAYHKSASALGGLTKIEDIEPLVRFLVTEGWWITGQ
CEEEEECCCCCCCCCCCCCCCCHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHCCEEEECE
TIFANGGYTTR
EEEECCCCCCC
>Mature Secondary Structure 
TAQTLKNKVVLITGGAKNLGGLISRKFAEQGAKLAIHYNSESTKADAEKTLADVQALGA
CCHHHCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCC
DAILLQGDLSSIDRIEALFVKTKAHFGGIDIAINTVGRVLKKPFLETTETEFDSMSDINS
CEEEEECCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
KIAYFFIQSAGRHLNDGGKICTIVTSLLAAYTGLYSTYEGLKAPVEHYTRAASKEFGERG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ISVTAVAPGPMDTPFFYGQEAPEAVAYHKSASALGGLTKIEDIEPLVRFLVTEGWWITGQ
CEEEEECCCCCCCCCCCCCCCCHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHCCEEEECE
TIFANGGYTTR
EEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA