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The map label for this gene is phaD [H]

Identifier: 50083634

GI number: 50083634

Start: 365250

End: 367058

Strand: Reverse

Name: phaD [H]

Synonym: ACIAD0374

Alternate gene names: 50083634

Gene position: 367058-365250 (Counterclockwise)

Preceding gene: 161349971

Following gene: 50083633

Centisome position: 10.2

GC content: 43.39

Gene sequence:

>1809_bases
ATGTCTGATTTCATCACTTTTTGGCATATGCATGCGCCGATCTTGAGTATTTTAATTCCAGCATTTACCGCATTTAGTCT
AGTTTTATTGGGCAATCCAGGCTCAGGGGCACTTGAACATGATCCACGCCAACCCTGGCGTCGGGGAATTAGTTTCATCT
CGATATTATTGGGCCTTGCAACCGCTATCAGCTATTTCATGATGGCCAGCACAGGTCAGATCACGGTATATCAGTTAGGT
GAATGGTCTGCACCATTTGGGATTGTACTGGTTCTTGATCGATTATCTGCCTTTATGGTGCTACTTACCTATGTACTCGC
TACACCAGTACTCTGGTTTGCAAGTAAAGAATGGGATGCACGTGGTCGCTATTTCCATGTGATGTACCATTTTTTACTGA
TGGGTGTATGTGGCGCATTTCTAACAGGCGATTTATTTAACCTGTTTGTATTTTTTGAGGTGCTACTACTTGCGTCTTAT
GTCTTACTGCTACACACCCAAGGTAAAGCCAGATTTCAGCTCGGAATTCATTACGTCACCATCAATTTGCTTGCATCGGC
ACTATTCCTGATTGGGCTAGGCATGATTTATGGCAATGTAGGCAGCTTAAATATGGCCGATGTTGCGCGTTTGGTGCCTA
TTCTTGAACCAGAACAACATAAGCTTGCGATTGCAGGGGGGTTATTACTATTTGTTGTTTTTGGCATCAAAGCTGCCATG
CTTCCTGTCGGGTTCTGGCTACCCAAGACCTATGCTGTCGCGACTACGCCAGTTGCTGCACTGTTTACCATTATGACTAA
AGTGGGGATTTACGCGATTTTGCGAGTCAATGGAACGGTCTTTGATGACGATTTCTCTCAAGCGATGTTGCAAAAATGGT
TACTTCCAATCGGTCTTGTTACCTCACTTTATGGCGTGATTGGTGCCATTGGTGCAGACCGCTTACGTCGTTTCATTGGC
TTCATGATTCTGTCATCCGTCGGAACCATTTTGATTGCAATTGCCATGTCAAATACCCAAGCATGGGCAGCCGCACTTTA
CTATCTAGTACATAGCACCCTGATTGCAGCCGCATTTTATTTATTTTCAGGCTGGATCACCTCACAACGTGGCGAATTTA
AAGATCATTTAAAAATTGCGCCTCAAATGAAACAAGAAAAAATTACCTCAATCACCTTTTTGGTCATTGCGCTGATGCTG
GCAGGCTTACCCCCTTTTAGTGGTTTCTGGGGTAAAGTATTTATCTTGCAATCGTCTGCACAATCGGCTTATCAAGGCTG
GATTATTGCGATCATTCTAATCGTTAGCCTGCTGAGTATTATTGCCCTGACACGTGTTGGTTTTGTATTATTTTGGCGGG
CTAGCCGTCCTGAAGATAATCCGAAAGAACCCGCTTATGCAGTTTATCAAGCTTTACCTGATCGCGCACCCGCACGTAAT
AATCAAACCATTTATGTACTGCTTGCTGGTCTAATTTTGTATGTCGTTTTTGCCGCACCTATTCAGCAATATACGCTTGC
AACTGCCGAACAAATTCGTGATCGTAGCTTATATATCAATCAAGTCCTAAAGAAAGATCGTGAAGGCAATGTGATTAGTG
TAGCAAGTTATGATCCGACTTATTTGCCCGAAACCAAATATGGCGGTGAAGCATTGGATAGTAATGCTCACTATATTCCC
TACATCATTTCGGAAAACTCACTAAAGGGTGAACATATTTCCCCTTATAAACAGCGTCAAATCCAGCAACAACTGAATAA
TGAAAGCGTTGCTCCAACTGATGCACAGCTCAAACCCATGGAGCCCTAG

Upstream 100 bases:

>100_bases
TGCAGCTTGCAATCCGTAGTCGCTATGAATCAGGAACTGATCATGTCGATTCTAAAGAAGATCCTGCATTAAACTATGAC
CCGCGTGAGGATAAGCCTTA

Downstream 100 bases:

>100_bases
TCATGAGTAAGCATTCCTTTATTGAGCGCTGGTTTCCACATCCTTTTGTTTCGGTGATTATTGCTCTAAGCTGGCTTATG
CTGGCACATAGCCTTGAATT

Product: monovalent cation/H+ antiporter subunit D

Products: H2; oxidized ferredoxin [C]

Alternate protein names: pH adaptation potassium efflux system protein D; Pha system subunit D [H]

Number of amino acids: Translated: 602; Mature: 601

Protein sequence:

>602_residues
MSDFITFWHMHAPILSILIPAFTAFSLVLLGNPGSGALEHDPRQPWRRGISFISILLGLATAISYFMMASTGQITVYQLG
EWSAPFGIVLVLDRLSAFMVLLTYVLATPVLWFASKEWDARGRYFHVMYHFLLMGVCGAFLTGDLFNLFVFFEVLLLASY
VLLLHTQGKARFQLGIHYVTINLLASALFLIGLGMIYGNVGSLNMADVARLVPILEPEQHKLAIAGGLLLFVVFGIKAAM
LPVGFWLPKTYAVATTPVAALFTIMTKVGIYAILRVNGTVFDDDFSQAMLQKWLLPIGLVTSLYGVIGAIGADRLRRFIG
FMILSSVGTILIAIAMSNTQAWAAALYYLVHSTLIAAAFYLFSGWITSQRGEFKDHLKIAPQMKQEKITSITFLVIALML
AGLPPFSGFWGKVFILQSSAQSAYQGWIIAIILIVSLLSIIALTRVGFVLFWRASRPEDNPKEPAYAVYQALPDRAPARN
NQTIYVLLAGLILYVVFAAPIQQYTLATAEQIRDRSLYINQVLKKDREGNVISVASYDPTYLPETKYGGEALDSNAHYIP
YIISENSLKGEHISPYKQRQIQQQLNNESVAPTDAQLKPMEP

Sequences:

>Translated_602_residues
MSDFITFWHMHAPILSILIPAFTAFSLVLLGNPGSGALEHDPRQPWRRGISFISILLGLATAISYFMMASTGQITVYQLG
EWSAPFGIVLVLDRLSAFMVLLTYVLATPVLWFASKEWDARGRYFHVMYHFLLMGVCGAFLTGDLFNLFVFFEVLLLASY
VLLLHTQGKARFQLGIHYVTINLLASALFLIGLGMIYGNVGSLNMADVARLVPILEPEQHKLAIAGGLLLFVVFGIKAAM
LPVGFWLPKTYAVATTPVAALFTIMTKVGIYAILRVNGTVFDDDFSQAMLQKWLLPIGLVTSLYGVIGAIGADRLRRFIG
FMILSSVGTILIAIAMSNTQAWAAALYYLVHSTLIAAAFYLFSGWITSQRGEFKDHLKIAPQMKQEKITSITFLVIALML
AGLPPFSGFWGKVFILQSSAQSAYQGWIIAIILIVSLLSIIALTRVGFVLFWRASRPEDNPKEPAYAVYQALPDRAPARN
NQTIYVLLAGLILYVVFAAPIQQYTLATAEQIRDRSLYINQVLKKDREGNVISVASYDPTYLPETKYGGEALDSNAHYIP
YIISENSLKGEHISPYKQRQIQQQLNNESVAPTDAQLKPMEP
>Mature_601_residues
SDFITFWHMHAPILSILIPAFTAFSLVLLGNPGSGALEHDPRQPWRRGISFISILLGLATAISYFMMASTGQITVYQLGE
WSAPFGIVLVLDRLSAFMVLLTYVLATPVLWFASKEWDARGRYFHVMYHFLLMGVCGAFLTGDLFNLFVFFEVLLLASYV
LLLHTQGKARFQLGIHYVTINLLASALFLIGLGMIYGNVGSLNMADVARLVPILEPEQHKLAIAGGLLLFVVFGIKAAML
PVGFWLPKTYAVATTPVAALFTIMTKVGIYAILRVNGTVFDDDFSQAMLQKWLLPIGLVTSLYGVIGAIGADRLRRFIGF
MILSSVGTILIAIAMSNTQAWAAALYYLVHSTLIAAAFYLFSGWITSQRGEFKDHLKIAPQMKQEKITSITFLVIALMLA
GLPPFSGFWGKVFILQSSAQSAYQGWIIAIILIVSLLSIIALTRVGFVLFWRASRPEDNPKEPAYAVYQALPDRAPARNN
QTIYVLLAGLILYVVFAAPIQQYTLATAEQIRDRSLYINQVLKKDREGNVISVASYDPTYLPETKYGGEALDSNAHYIPY
IISENSLKGEHISPYKQRQIQQQLNNESVAPTDAQLKPMEP

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=212, Percent_Identity=25.9433962264151, Blast_Score=73, Evalue=7e-13,
Organism=Escherichia coli, GI1788827, Length=409, Percent_Identity=26.8948655256724, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788613, Length=375, Percent_Identity=24.8, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788829, Length=421, Percent_Identity=23.7529691211401, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI145693160, Length=349, Percent_Identity=24.0687679083095, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 66749; Mature: 66618

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFITFWHMHAPILSILIPAFTAFSLVLLGNPGSGALEHDPRQPWRRGISFISILLGLA
CCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
TAISYFMMASTGQITVYQLGEWSAPFGIVLVLDRLSAFMVLLTYVLATPVLWFASKEWDA
HHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RGRYFHVMYHFLLMGVCGAFLTGDLFNLFVFFEVLLLASYVLLLHTQGKARFQLGIHYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHH
INLLASALFLIGLGMIYGNVGSLNMADVARLVPILEPEQHKLAIAGGLLLFVVFGIKAAM
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
LPVGFWLPKTYAVATTPVAALFTIMTKVGIYAILRVNGTVFDDDFSQAMLQKWLLPIGLV
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCEEECCHHHHHHHHHHHHHHHHH
TSLYGVIGAIGADRLRRFIGFMILSSVGTILIAIAMSNTQAWAAALYYLVHSTLIAAAFY
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHH
LFSGWITSQRGEFKDHLKIAPQMKQEKITSITFLVIALMLAGLPPFSGFWGKVFILQSSA
HHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCH
QSAYQGWIIAIILIVSLLSIIALTRVGFVLFWRASRPEDNPKEPAYAVYQALPDRAPARN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHCCCCCCCCC
NQTIYVLLAGLILYVVFAAPIQQYTLATAEQIRDRSLYINQVLKKDREGNVISVASYDPT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
YLPETKYGGEALDSNAHYIPYIISENSLKGEHISPYKQRQIQQQLNNESVAPTDAQLKPM
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
EP
CC
>Mature Secondary Structure 
SDFITFWHMHAPILSILIPAFTAFSLVLLGNPGSGALEHDPRQPWRRGISFISILLGLA
CCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
TAISYFMMASTGQITVYQLGEWSAPFGIVLVLDRLSAFMVLLTYVLATPVLWFASKEWDA
HHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RGRYFHVMYHFLLMGVCGAFLTGDLFNLFVFFEVLLLASYVLLLHTQGKARFQLGIHYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHH
INLLASALFLIGLGMIYGNVGSLNMADVARLVPILEPEQHKLAIAGGLLLFVVFGIKAAM
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
LPVGFWLPKTYAVATTPVAALFTIMTKVGIYAILRVNGTVFDDDFSQAMLQKWLLPIGLV
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCEEECCHHHHHHHHHHHHHHHHH
TSLYGVIGAIGADRLRRFIGFMILSSVGTILIAIAMSNTQAWAAALYYLVHSTLIAAAFY
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHH
LFSGWITSQRGEFKDHLKIAPQMKQEKITSITFLVIALMLAGLPPFSGFWGKVFILQSSA
HHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCH
QSAYQGWIIAIILIVSLLSIIALTRVGFVLFWRASRPEDNPKEPAYAVYQALPDRAPARN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHCCCCCCCCC
NQTIYVLLAGLILYVVFAAPIQQYTLATAEQIRDRSLYINQVLKKDREGNVISVASYDPT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
YLPETKYGGEALDSNAHYIPYIISENSLKGEHISPYKQRQIQQQLNNESVAPTDAQLKPM
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
EP
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]