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The map label for this gene is phaF [H]

Identifier: 50083632

GI number: 50083632

Start: 364448

End: 364723

Strand: Reverse

Name: phaF [H]

Synonym: ACIAD0372

Alternate gene names: 50083632

Gene position: 364723-364448 (Counterclockwise)

Preceding gene: 50083633

Following gene: 50083631

Centisome position: 10.14

GC content: 44.2

Gene sequence:

>276_bases
ATGACCATTCTTCCATACGCATTAGGCATCTGCTTGATTGCCATTACGATTTCGATGTTTATGTGTCTGATCCGCTTAGT
CCTTGGTCCCTCAGTCATTGATCGGCTACTGGCACTAGATACACTCTTTTTAAATGCAACCTGTTTAGTGATCGTACTTG
GAATTTACTGGGCCACCACCAATCTGTTTGAAGGTGCCTTGCTGATTGCCATGCTTGGTTTTGTATCTACCGCAGCACTG
GCCCGTTATTTTACAACGGGTCATGTAATTGACTAG

Upstream 100 bases:

>100_bases
GTACCGAGAATACCGAACTCGATATTCAAGAGATTAAAAACCGTTATGAAGCACCATTAATTGCCATCTTTGATGTTGAT
GTAAATCAAGGAGATCCTAA

Downstream 100 bases:

>100_bases
GAGGCTATTATGCAATTGATTATGGAAATTTTGGTATCCATCTTTCTGATTTTAGGTGCATTTTTTACTCTAGTCGGTGG
TATTGGCCTGATCCGTCTGC

Product: pH adaptation potassium efflux system protein F

Products: NA

Alternate protein names: pH adaptation potassium efflux system protein F; Pha system subunit F [H]

Number of amino acids: Translated: 91; Mature: 90

Protein sequence:

>91_residues
MTILPYALGICLIAITISMFMCLIRLVLGPSVIDRLLALDTLFLNATCLVIVLGIYWATTNLFEGALLIAMLGFVSTAAL
ARYFTTGHVID

Sequences:

>Translated_91_residues
MTILPYALGICLIAITISMFMCLIRLVLGPSVIDRLLALDTLFLNATCLVIVLGIYWATTNLFEGALLIAMLGFVSTAAL
ARYFTTGHVID
>Mature_90_residues
TILPYALGICLIAITISMFMCLIRLVLGPSVIDRLLALDTLFLNATCLVIVLGIYWATTNLFEGALLIAMLGFVSTAALA
RYFTTGHVID

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG2212

COG function: function code P; Multisubunit Na+/H+ antiporter, MnhF subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit F family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007208 [H]

Pfam domain/function: PF04066 MrpF_PhaF [H]

EC number: NA

Molecular weight: Translated: 9867; Mature: 9736

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILPYALGICLIAITISMFMCLIRLVLGPSVIDRLLALDTLFLNATCLVIVLGIYWATT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLFEGALLIAMLGFVSTAALARYFTTGHVID
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TILPYALGICLIAITISMFMCLIRLVLGPSVIDRLLALDTLFLNATCLVIVLGIYWATT
CHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLFEGALLIAMLGFVSTAALARYFTTGHVID
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]